Click here to switch to the map view.

The map label for this gene is outD [H]

Identifier: 157963580

GI number: 157963580

Start: 4581935

End: 4583605

Strand: Reverse

Name: outD [H]

Synonym: Spea_3768

Alternate gene names: 157963580

Gene position: 4583605-4581935 (Counterclockwise)

Preceding gene: 157963581

Following gene: 157963579

Centisome position: 88.58

GC content: 47.46

Gene sequence:

>1671_bases
ATGCGCTCAATGACCTATATCACCCCTTTGCTGTCTTTATGTTTAGTGGCCTGCCAAACCGTCGACAGACCCAATCCTGT
AGAGTCTAAGCAAGCTCTGGCAGAATCTCTAACGACTAAAGAAGTGGCTGCTGTGACGCCACCGCCAGCAGTGATGCCCG
ATGATGTTCAACGTGAACTGTCATCAAACAATTTGTTAGAAGGCTTTGCCCCAGCAAGACCCAGCGAACGTCGCTTCGAT
GTGTCAGCTAATAATGTCGATGCTAAAGTATTCTTCCCAAGCCTTGCTCAAGGTACCCCCTTAAGTGTCGCTGTGCATCC
AGACGTGACAGGCAAGATCTCGCTGTCATTAAAGAGCGTGACCTTAAAAGAAGCACTACAAGTTGTAGAAGATATTTACG
GCTATGAAGTGAGCCGCGAAGGACGCATATTGCGTATTTTCCCTGCAGGTATGCGCACAGAAACGTTCTCGTTAAATTAC
CTGTATATGGAGCGTCAAGGGCTGTCTTTGACTTCGGTGAACTCTGGTCGTATTTCAGATAATAACTCCAACTCTGGTAG
CAATAGTAATAGCAACAGTAATAATAATTTCAACGATTCATCGAACTCGAGTTCTAGCTCTGATAGCAGCAATAGCAACG
AGCAAACCAACGGCACCTTTATTCGCTCTAAGACCAAGTCTGATTTCTGGGGTGAATTAAAAGAGACCTTGGTGTCTATC
GTGGGGAACACCGGCGGTGGACGCCAAGTGGTAGTGACTCCTCAAGCCGGTCTAGTGACCATTAGGGCTTACCCTAACGA
GATCAGACAGGTTCGAAGCTTTATCAAGACCGCGGAAAGTCATCTGCAGCGCCAAGTTATCTTAGAAGCCAAAGTGCTCG
AGGTGACCCTATCTGACGGCTACCAGCAAGGCATTCACTGGGAAAGTGTATTAGGTCACGCAGGTAGTACCGATATTAGC
TTTGGCACCAGCCCTGCAGCAGGGCTTGGGGACACCATTACTAATGCTTTGGGCGGAGTGACCTCAATTAAGCTAGAGGG
CTCAGATTTTAGCACCATGATAAGCCTGCTCGATACTCAGGGTGATGTTGATGTGTTATCTAGTCCGCGTGTCACAGCGT
CGAATAACCAAAAAGCGGTCATAAAAGTCGGTACAGACGAGTACTTTGTTACAGACGTTTCTTCAACTACAGTGGCAGGT
AATACGCCTGTGACGACCCCTGAGGTTGAACTGACTCCTTTCTTCTCTGGCATAGCCTTAGATGTGACGCCACAGATTGA
TGAGCAGGGCAACGTGCTACTGCATATTCACCCATCGGTGATCGATATTAAAGAGCAGGTAAAGAGCATTAAGATCGCAG
ATAGCACGCTTGAGCTACCACTAGCACAGAGTGAGATCCGTGAGTCAGACACTGTGATAAAGGCCGCGAGCGGTGATGTT
GTGGTGATTGGTGGTTTGATGAAGAGTGAAAATATTGAACTGGTTTCTAAGGTTCCGCTACTGGGTGATATTCCATTCCT
AGGTGAGGCCTTTACTAACCGTGCCAATTCGACGGTTAAAACCGAGTTAGTGATCTTGCTTAAACCAATCGTTGTCGGTG
CCAATACTTGGGAGAACGAACTTAAGCGTTCTCAAGAGCTACTAGAACGCTGGTATCCAGAGGAGCAGTAA

Upstream 100 bases:

>100_bases
GTCAAAATTGTGCATATTGGTATAGACAGTGTGTCGTTAGCCGACGGCCGAAAACTGACAATGTTTCAGGCGATTACAGA
CTTTAAGGGACATAATTAAC

Downstream 100 bases:

>100_bases
GCTTGTATCTGCAGCATTTTGGATTAACTGAAACCCCGTTTTCGCTTACGCCGAATACGGGATTTTTCTTTGGTCTCGCG
CCGCACGTCGAAGCCTTACA

Product: pilus (MSHA type) biogenesis protein MshL

Products: NA

Alternate protein names: Pectic enzymes secretion protein outD [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MRSMTYITPLLSLCLVACQTVDRPNPVESKQALAESLTTKEVAAVTPPPAVMPDDVQRELSSNNLLEGFAPARPSERRFD
VSANNVDAKVFFPSLAQGTPLSVAVHPDVTGKISLSLKSVTLKEALQVVEDIYGYEVSREGRILRIFPAGMRTETFSLNY
LYMERQGLSLTSVNSGRISDNNSNSGSNSNSNSNNNFNDSSNSSSSSDSSNSNEQTNGTFIRSKTKSDFWGELKETLVSI
VGNTGGGRQVVVTPQAGLVTIRAYPNEIRQVRSFIKTAESHLQRQVILEAKVLEVTLSDGYQQGIHWESVLGHAGSTDIS
FGTSPAAGLGDTITNALGGVTSIKLEGSDFSTMISLLDTQGDVDVLSSPRVTASNNQKAVIKVGTDEYFVTDVSSTTVAG
NTPVTTPEVELTPFFSGIALDVTPQIDEQGNVLLHIHPSVIDIKEQVKSIKIADSTLELPLAQSEIRESDTVIKAASGDV
VVIGGLMKSENIELVSKVPLLGDIPFLGEAFTNRANSTVKTELVILLKPIVVGANTWENELKRSQELLERWYPEEQ

Sequences:

>Translated_556_residues
MRSMTYITPLLSLCLVACQTVDRPNPVESKQALAESLTTKEVAAVTPPPAVMPDDVQRELSSNNLLEGFAPARPSERRFD
VSANNVDAKVFFPSLAQGTPLSVAVHPDVTGKISLSLKSVTLKEALQVVEDIYGYEVSREGRILRIFPAGMRTETFSLNY
LYMERQGLSLTSVNSGRISDNNSNSGSNSNSNSNNNFNDSSNSSSSSDSSNSNEQTNGTFIRSKTKSDFWGELKETLVSI
VGNTGGGRQVVVTPQAGLVTIRAYPNEIRQVRSFIKTAESHLQRQVILEAKVLEVTLSDGYQQGIHWESVLGHAGSTDIS
FGTSPAAGLGDTITNALGGVTSIKLEGSDFSTMISLLDTQGDVDVLSSPRVTASNNQKAVIKVGTDEYFVTDVSSTTVAG
NTPVTTPEVELTPFFSGIALDVTPQIDEQGNVLLHIHPSVIDIKEQVKSIKIADSTLELPLAQSEIRESDTVIKAASGDV
VVIGGLMKSENIELVSKVPLLGDIPFLGEAFTNRANSTVKTELVILLKPIVVGANTWENELKRSQELLERWYPEEQ
>Mature_556_residues
MRSMTYITPLLSLCLVACQTVDRPNPVESKQALAESLTTKEVAAVTPPPAVMPDDVQRELSSNNLLEGFAPARPSERRFD
VSANNVDAKVFFPSLAQGTPLSVAVHPDVTGKISLSLKSVTLKEALQVVEDIYGYEVSREGRILRIFPAGMRTETFSLNY
LYMERQGLSLTSVNSGRISDNNSNSGSNSNSNSNNNFNDSSNSSSSSDSSNSNEQTNGTFIRSKTKSDFWGELKETLVSI
VGNTGGGRQVVVTPQAGLVTIRAYPNEIRQVRSFIKTAESHLQRQVILEAKVLEVTLSDGYQQGIHWESVLGHAGSTDIS
FGTSPAAGLGDTITNALGGVTSIKLEGSDFSTMISLLDTQGDVDVLSSPRVTASNNQKAVIKVGTDEYFVTDVSSTTVAG
NTPVTTPEVELTPFFSGIALDVTPQIDEQGNVLLHIHPSVIDIKEQVKSIKIADSTLELPLAQSEIRESDTVIKAASGDV
VVIGGLMKSENIELVSKVPLLGDIPFLGEAFTNRANSTVKTELVILLKPIVVGANTWENELKRSQELLERWYPEEQ

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=291, Percent_Identity=27.4914089347079, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1789793, Length=478, Percent_Identity=24.0585774058577, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 59995; Mature: 59995

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSMTYITPLLSLCLVACQTVDRPNPVESKQALAESLTTKEVAAVTPPPAVMPDDVQREL
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHH
SSNNLLEGFAPARPSERRFDVSANNVDAKVFFPSLAQGTPLSVAVHPDVTGKISLSLKSV
CCCCCCCCCCCCCCCCCEEECCCCCCEEEEEECCCCCCCCEEEEECCCCCEEEEEEEEHH
TLKEALQVVEDIYGYEVSREGRILRIFPAGMRTETFSLNYLYMERQGLSLTSVNSGRISD
HHHHHHHHHHHHHCCEECCCCCEEEEECCCCCEEEEEEEEEEEECCCCEEEECCCCCCCC
NNSNSGSNSNSNSNNNFNDSSNSSSSSDSSNSNEQTNGTFIRSKTKSDFWGELKETLVSI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
VGNTGGGRQVVVTPQAGLVTIRAYPNEIRQVRSFIKTAESHLQRQVILEAKVLEVTLSDG
HCCCCCCCEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCC
YQQGIHWESVLGHAGSTDISFGTSPAAGLGDTITNALGGVTSIKLEGSDFSTMISLLDTQ
HHHCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHCC
GDVDVLSSPRVTASNNQKAVIKVGTDEYFVTDVSSTTVAGNTPVTTPEVELTPFFSGIAL
CCEEECCCCCEEECCCCEEEEEECCCCEEEEECCCCEECCCCCCCCCCEEEEECCCCEEE
DVTPQIDEQGNVLLHIHPSVIDIKEQVKSIKIADSTLELPLAQSEIRESDTVIKAASGDV
EECCCCCCCCCEEEEECCCHHHHHHHHHHEEEECCCCCCCCCHHHHHCCCCEEEECCCCE
VVIGGLMKSENIELVSKVPLLGDIPFLGEAFTNRANSTVKTELVILLKPIVVGANTWENE
EEEECEECCCCCEEEECCCCCCCCCCHHHHHHCCCCCCHHHEEEEEEECCEECCCCHHHH
LKRSQELLERWYPEEQ
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRSMTYITPLLSLCLVACQTVDRPNPVESKQALAESLTTKEVAAVTPPPAVMPDDVQREL
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHH
SSNNLLEGFAPARPSERRFDVSANNVDAKVFFPSLAQGTPLSVAVHPDVTGKISLSLKSV
CCCCCCCCCCCCCCCCCEEECCCCCCEEEEEECCCCCCCCEEEEECCCCCEEEEEEEEHH
TLKEALQVVEDIYGYEVSREGRILRIFPAGMRTETFSLNYLYMERQGLSLTSVNSGRISD
HHHHHHHHHHHHHCCEECCCCCEEEEECCCCCEEEEEEEEEEEECCCCEEEECCCCCCCC
NNSNSGSNSNSNSNNNFNDSSNSSSSSDSSNSNEQTNGTFIRSKTKSDFWGELKETLVSI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
VGNTGGGRQVVVTPQAGLVTIRAYPNEIRQVRSFIKTAESHLQRQVILEAKVLEVTLSDG
HCCCCCCCEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCC
YQQGIHWESVLGHAGSTDISFGTSPAAGLGDTITNALGGVTSIKLEGSDFSTMISLLDTQ
HHHCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHCC
GDVDVLSSPRVTASNNQKAVIKVGTDEYFVTDVSSTTVAGNTPVTTPEVELTPFFSGIAL
CCEEECCCCCEEECCCCEEEEEECCCCEEEEECCCCEECCCCCCCCCCEEEEECCCCEEE
DVTPQIDEQGNVLLHIHPSVIDIKEQVKSIKIADSTLELPLAQSEIRESDTVIKAASGDV
EECCCCCCCCCEEEEECCCHHHHHHHHHHEEEECCCCCCCCCHHHHHCCCCEEEECCCCE
VVIGGLMKSENIELVSKVPLLGDIPFLGEAFTNRANSTVKTELVILLKPIVVGANTWENE
EEEECEECCCCCEEEECCCCCCCCCCHHHHHHCCCCCCHHHEEEEEEECCEECCCCHHHH
LKRSQELLERWYPEEQ
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]