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The map label for this gene is ycaN [H]

Identifier: 157963506

GI number: 157963506

Start: 4493594

End: 4494544

Strand: Reverse

Name: ycaN [H]

Synonym: Spea_3694

Alternate gene names: 157963506

Gene position: 4494544-4493594 (Counterclockwise)

Preceding gene: 157963511

Following gene: 157963502

Centisome position: 86.86

GC content: 49.53

Gene sequence:

>951_bases
ATGAAAACAGAAGATATTGCCCTATTCCATCGAATTGTCGAAACCGGTAGTTTAGTCGAGGCGGCAGACATACTAAACCT
GCCAAAATCGACCCTGAGCCGACGCTTGCAGGCACTCGAAGACGAACTCAATGTGAAACTGTTTCATCGCCAGAGTCGCG
CCATGACCTTAACCGCATCGGGTAGCCATTTCTACGATAAAAGTACCGCTATGTTGGCAACGCTGGAGCAGACGCTATCT
GAGCTCAGCGGTGAAGAATCGGAGGTGACGGGACACCTGCGCATCTTAATCTTTCCGGTGCCAGAGCTTATCCATATTAC
CCATGCTATTTTTGAGTTTATGGATCTGCACCCCGAGCTAACGGTAGAGATCATCGTCAGTACTGAGCCGCAAGATATGA
TCCGCAACAACATCGACTTGGCCTTTATGCTCGAAGATGCCTTCAATGAAAACGAGATGGTGGCGCGGCACGTCATTAGC
GAAATGGTGCACTTCTTTGCCAGTCCAGAATACTTAGCAAAAGCGGGGCGTCCCACAAGTCCCGATGAGTTACCGCAGCA
CAACTCCATTCTGTTCCGTTACCCAAATGGGCGGATTTTTAATGAGGTGCCCTTTGGTAAAGACCGCACCCTGTCGGTAA
AGGGCAACTTGTGTGTAAATAGCCTTGCGACTTGTTTAGAGGCGACCCTGATGGGACGGGGGATCTCCATGATGCCATTG
CCTTTCGCTAAAGAGCATATCGCTAGTGGAGAGCTAGAGATGCTGTTTGAAGATGTCGAGCCCTACGAGGGCAAGTGCTT
CTTAGTGTATCCATCGAGACGCTTTATTAGCTTGGCGAGTCAACGATTTATTGACTATATGATGACTGCGATGGAAGAGT
GCATGGGTAGTGGCAGTGGTCGATGTGACAGAGAAGTGAAGATCCGCGGGGCGATCAAGTCGATGATTTAA

Upstream 100 bases:

>100_bases
TTCCGAAAAATGGAACTTCTTTTGCTTGCTTTGCCGTCACAACTGTGAGCAAAGTGATAGTTGTCCGTAGTCAGTACGTT
AAATGCTAGAGATTTTTTAG

Downstream 100 bases:

>100_bases
AACCGAGGATTAACATGAATAATCTAACTTTGAATTGTTAGCCATCAAGTCCAAAAATATCAATGACAAAAATAGCGCCA
ATGCCACTTCTCAGTGACAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MKTEDIALFHRIVETGSLVEAADILNLPKSTLSRRLQALEDELNVKLFHRQSRAMTLTASGSHFYDKSTAMLATLEQTLS
ELSGEESEVTGHLRILIFPVPELIHITHAIFEFMDLHPELTVEIIVSTEPQDMIRNNIDLAFMLEDAFNENEMVARHVIS
EMVHFFASPEYLAKAGRPTSPDELPQHNSILFRYPNGRIFNEVPFGKDRTLSVKGNLCVNSLATCLEATLMGRGISMMPL
PFAKEHIASGELEMLFEDVEPYEGKCFLVYPSRRFISLASQRFIDYMMTAMEECMGSGSGRCDREVKIRGAIKSMI

Sequences:

>Translated_316_residues
MKTEDIALFHRIVETGSLVEAADILNLPKSTLSRRLQALEDELNVKLFHRQSRAMTLTASGSHFYDKSTAMLATLEQTLS
ELSGEESEVTGHLRILIFPVPELIHITHAIFEFMDLHPELTVEIIVSTEPQDMIRNNIDLAFMLEDAFNENEMVARHVIS
EMVHFFASPEYLAKAGRPTSPDELPQHNSILFRYPNGRIFNEVPFGKDRTLSVKGNLCVNSLATCLEATLMGRGISMMPL
PFAKEHIASGELEMLFEDVEPYEGKCFLVYPSRRFISLASQRFIDYMMTAMEECMGSGSGRCDREVKIRGAIKSMI
>Mature_316_residues
MKTEDIALFHRIVETGSLVEAADILNLPKSTLSRRLQALEDELNVKLFHRQSRAMTLTASGSHFYDKSTAMLATLEQTLS
ELSGEESEVTGHLRILIFPVPELIHITHAIFEFMDLHPELTVEIIVSTEPQDMIRNNIDLAFMLEDAFNENEMVARHVIS
EMVHFFASPEYLAKAGRPTSPDELPQHNSILFRYPNGRIFNEVPFGKDRTLSVKGNLCVNSLATCLEATLMGRGISMMPL
PFAKEHIASGELEMLFEDVEPYEGKCFLVYPSRRFISLASQRFIDYMMTAMEECMGSGSGRCDREVKIRGAIKSMI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=28.8659793814433, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI145693193, Length=299, Percent_Identity=28.7625418060201, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=29.4117647058824, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1787589, Length=293, Percent_Identity=23.5494880546075, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789639, Length=285, Percent_Identity=24.2105263157895, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789440, Length=302, Percent_Identity=24.8344370860927, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87081978, Length=279, Percent_Identity=25.8064516129032, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788706, Length=174, Percent_Identity=27.0114942528736, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI145693105, Length=117, Percent_Identity=27.3504273504274, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35683; Mature: 35683

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTEDIALFHRIVETGSLVEAADILNLPKSTLSRRLQALEDELNVKLFHRQSRAMTLTAS
CCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEECCCEEEEEEC
GSHFYDKSTAMLATLEQTLSELSGEESEVTGHLRILIFPVPELIHITHAIFEFMDLHPEL
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCE
TVEIIVSTEPQDMIRNNIDLAFMLEDAFNENEMVARHVISEMVHFFASPEYLAKAGRPTS
EEEEEEECCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCC
PDELPQHNSILFRYPNGRIFNEVPFGKDRTLSVKGNLCVNSLATCLEATLMGRGISMMPL
CHHCCCCCCEEEECCCCCEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCC
PFAKEHIASGELEMLFEDVEPYEGKCFLVYPSRRFISLASQRFIDYMMTAMEECMGSGSG
CCHHHHHCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RCDREVKIRGAIKSMI
CCCCCEEHHHHHHHCC
>Mature Secondary Structure
MKTEDIALFHRIVETGSLVEAADILNLPKSTLSRRLQALEDELNVKLFHRQSRAMTLTAS
CCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEECCCEEEEEEC
GSHFYDKSTAMLATLEQTLSELSGEESEVTGHLRILIFPVPELIHITHAIFEFMDLHPEL
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCE
TVEIIVSTEPQDMIRNNIDLAFMLEDAFNENEMVARHVISEMVHFFASPEYLAKAGRPTS
EEEEEEECCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCC
PDELPQHNSILFRYPNGRIFNEVPFGKDRTLSVKGNLCVNSLATCLEATLMGRGISMMPL
CHHCCCCCCEEEECCCCCEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCC
PFAKEHIASGELEMLFEDVEPYEGKCFLVYPSRRFISLASQRFIDYMMTAMEECMGSGSG
CCHHHHHCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RCDREVKIRGAIKSMI
CCCCCEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]