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The map label for this gene is opuD [H]

Identifier: 157963486

GI number: 157963486

Start: 4471892

End: 4473541

Strand: Reverse

Name: opuD [H]

Synonym: Spea_3674

Alternate gene names: 157963486

Gene position: 4473541-4471892 (Counterclockwise)

Preceding gene: 157963487

Following gene: 157963483

Centisome position: 86.45

GC content: 48.24

Gene sequence:

>1650_bases
ATGGCTGACGACGATAAAAGCGAATACTTAATGTCGAATTTGACACAATCTGTTGAGCGTAAATCAACTGATGAAAATTA
CCTTGCGGCGCATCCGCCTGAGCGAAGTGATGCATCAACCACTAACGCCTTTGCCAGTCCTGTACTCTGGTTAAGTGGCG
GCTTTCTCGTTCTCTTCGTACTGCTTGCTGTTTTCGATACCTCCGCTCTGTCCAGCGTCATTCAAGTTGGATTCTCAAAA
GTGACCCTATGGTTTGGCCCTTTTTGGCAAGTTCTGCTGTTGGCTAACTTTGTGATTGGTCTCTACTTAGCCTTTGCTAG
AACTGGCGATGTTACCTTGGGTAACCTTAAAGCGCCGGAAATGGATACCTTCAAATGGTTGGCTATTATCCTGTGTACCC
TATTAGCGGGTGGTGGTGTATTTTGGGCGGCGGCAGAGCCTATCGCTCACTTTGTTTCTTCGCCGCCATTTTATGGTGAA
GCTGAAGGTCGCAGGCTGGCAATCAATGCGTTATCGCAATCATTCCTGCATTGGGGCTTCTTAGCGTGGGCGATACTCGG
TAGCTTGGCGGCAATCGTGTTAATGCATCTGCACTACGACAAAGGCTTACCGTTGCAACCTCGCACCTTGTTGTATCCCA
TTTTTGGCGACAGAGCGCTTAATAGCTGGGTTGGTACCTTAACCGACACTTTCTGTATTATTGCAGTGGCTGCGGGCACC
ATTGGCCCTATCGGCTTCCTTGGCCTGCAAATCAGCTACGCACTCAATGAACTTTTTGGTGTGCAAGATACCTTAATCAC
CCAAAGTTTGGTGGTAGTGTTTGCTATCGCCATGTATACCTTGTCGGCATTGAGCGGCCTTAGCCGCGGCATTCAGCTAG
TTAGCCGCTACAATATTTTACTTTCAGTCGCACTCATCATCTTTATTCTATTATTTGGCCCAACCAGCTTTATTGTTGAT
GGTTACATGCAAGGCGTGGCGAGAATGGTGAATGATTTTGTGCCTATGTCGCTCTATCGTGGTGATGCCAACTGGCTAAG
TTGGTGGACGGTATTTTTCTGGGGCTGGTTTATTGGTTATGGCCCTATGATGGCTATTTTCATTGCGCGTATTTCTCGTG
GCCGCACGATTCGCCAATTGATCCTGTCGATTTCTATTATTGCACCGCTAATCACCTGTTTCTGGTTTAGTGTTGTTGGT
GGTTCAGGTCTAGCATTTGAGCTCGCTAACCCTGGTGTTATTGCTAATGCATTTGAAGGCTTTAACTTACCAGGCGTGTT
GCTGGCGATTACTTCACAGCTGCCAATGCCGACGCTGATAGCGATACTGTTTTTGATCCTTACGACTACCTTCATTGTTA
CTACGGGTGACTCGATGACTTACACCATTAGTGTGGTTGTCACCGGTTCTACCGAGCCTAGTGGGGCAATGCGTGCGTTT
TGGGGCATATTGATGGGGCTGGTTGCTATCGCGCTAATTGCGATGGGCTCGGGCGGCATTTCCGCCTTACAATCCTTTAT
TGTGATCACCGCCGTTCCGGTCTCTTTTGTGCTACTACCTACTTTGTGGCAAGCGCCTAAAATGGCGAAAGCGATGGCGG
AGCAGCAATTTGGCAAAGCCTCGCTTAATCAACAAGCAGAGCGCTCGTAG

Upstream 100 bases:

>100_bases
ACAGCCTCAACCTTTACAAAGTCGCTATAAGTCTTTGCGATAGCCGAGGAAAATTCACCGAATCAGCTTAAAAATAGTAG
CGCTAAACGGCGCTCATTTA

Downstream 100 bases:

>100_bases
GCAAGAAAGCTAATGTCCACAGCATAAAATTGCTTTTTAAAGCCTCGTTACTGAATAAGTATCGGGGCTTTTTTATTGCG
GCTAGCCCCGAGGTTTTTAA

Product: BCCT transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MADDDKSEYLMSNLTQSVERKSTDENYLAAHPPERSDASTTNAFASPVLWLSGGFLVLFVLLAVFDTSALSSVIQVGFSK
VTLWFGPFWQVLLLANFVIGLYLAFARTGDVTLGNLKAPEMDTFKWLAIILCTLLAGGGVFWAAAEPIAHFVSSPPFYGE
AEGRRLAINALSQSFLHWGFLAWAILGSLAAIVLMHLHYDKGLPLQPRTLLYPIFGDRALNSWVGTLTDTFCIIAVAAGT
IGPIGFLGLQISYALNELFGVQDTLITQSLVVVFAIAMYTLSALSGLSRGIQLVSRYNILLSVALIIFILLFGPTSFIVD
GYMQGVARMVNDFVPMSLYRGDANWLSWWTVFFWGWFIGYGPMMAIFIARISRGRTIRQLILSISIIAPLITCFWFSVVG
GSGLAFELANPGVIANAFEGFNLPGVLLAITSQLPMPTLIAILFLILTTTFIVTTGDSMTYTISVVVTGSTEPSGAMRAF
WGILMGLVAIALIAMGSGGISALQSFIVITAVPVSFVLLPTLWQAPKMAKAMAEQQFGKASLNQQAERS

Sequences:

>Translated_549_residues
MADDDKSEYLMSNLTQSVERKSTDENYLAAHPPERSDASTTNAFASPVLWLSGGFLVLFVLLAVFDTSALSSVIQVGFSK
VTLWFGPFWQVLLLANFVIGLYLAFARTGDVTLGNLKAPEMDTFKWLAIILCTLLAGGGVFWAAAEPIAHFVSSPPFYGE
AEGRRLAINALSQSFLHWGFLAWAILGSLAAIVLMHLHYDKGLPLQPRTLLYPIFGDRALNSWVGTLTDTFCIIAVAAGT
IGPIGFLGLQISYALNELFGVQDTLITQSLVVVFAIAMYTLSALSGLSRGIQLVSRYNILLSVALIIFILLFGPTSFIVD
GYMQGVARMVNDFVPMSLYRGDANWLSWWTVFFWGWFIGYGPMMAIFIARISRGRTIRQLILSISIIAPLITCFWFSVVG
GSGLAFELANPGVIANAFEGFNLPGVLLAITSQLPMPTLIAILFLILTTTFIVTTGDSMTYTISVVVTGSTEPSGAMRAF
WGILMGLVAIALIAMGSGGISALQSFIVITAVPVSFVLLPTLWQAPKMAKAMAEQQFGKASLNQQAERS
>Mature_548_residues
ADDDKSEYLMSNLTQSVERKSTDENYLAAHPPERSDASTTNAFASPVLWLSGGFLVLFVLLAVFDTSALSSVIQVGFSKV
TLWFGPFWQVLLLANFVIGLYLAFARTGDVTLGNLKAPEMDTFKWLAIILCTLLAGGGVFWAAAEPIAHFVSSPPFYGEA
EGRRLAINALSQSFLHWGFLAWAILGSLAAIVLMHLHYDKGLPLQPRTLLYPIFGDRALNSWVGTLTDTFCIIAVAAGTI
GPIGFLGLQISYALNELFGVQDTLITQSLVVVFAIAMYTLSALSGLSRGIQLVSRYNILLSVALIIFILLFGPTSFIVDG
YMQGVARMVNDFVPMSLYRGDANWLSWWTVFFWGWFIGYGPMMAIFIARISRGRTIRQLILSISIIAPLITCFWFSVVGG
SGLAFELANPGVIANAFEGFNLPGVLLAITSQLPMPTLIAILFLILTTTFIVTTGDSMTYTISVVVTGSTEPSGAMRAFW
GILMGLVAIALIAMGSGGISALQSFIVITAVPVSFVLLPTLWQAPKMAKAMAEQQFGKASLNQQAERS

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=525, Percent_Identity=30.4761904761905, Blast_Score=208, Evalue=7e-55,
Organism=Escherichia coli, GI1788102, Length=445, Percent_Identity=27.6404494382022, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1786224, Length=464, Percent_Identity=26.9396551724138, Blast_Score=159, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 59497; Mature: 59365

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADDDKSEYLMSNLTQSVERKSTDENYLAAHPPERSDASTTNAFASPVLWLSGGFLVLFV
CCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCHHHHHCCHHHHCCCHHHHHH
LLAVFDTSALSSVIQVGFSKVTLWFGPFWQVLLLANFVIGLYLAFARTGDVTLGNLKAPE
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCC
MDTFKWLAIILCTLLAGGGVFWAAAEPIAHFVSSPPFYGEAEGRRLAINALSQSFLHWGF
CHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHH
LAWAILGSLAAIVLMHLHYDKGLPLQPRTLLYPIFGDRALNSWVGTLTDTFCIIAVAAGT
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCC
IGPIGFLGLQISYALNELFGVQDTLITQSLVVVFAIAMYTLSALSGLSRGIQLVSRYNIL
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSVALIIFILLFGPTSFIVDGYMQGVARMVNDFVPMSLYRGDANWLSWWTVFFWGWFIGY
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHH
GPMMAIFIARISRGRTIRQLILSISIIAPLITCFWFSVVGGSGLAFELANPGVIANAFEG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEHHHCC
FNLPGVLLAITSQLPMPTLIAILFLILTTTFIVTTGDSMTYTISVVVTGSTEPSGAMRAF
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHEECCCCEEEEEEEEEECCCCCCHHHHHH
WGILMGLVAIALIAMGSGGISALQSFIVITAVPVSFVLLPTLWQAPKMAKAMAEQQFGKA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHH
SLNQQAERS
HHCHHHCCC
>Mature Secondary Structure 
ADDDKSEYLMSNLTQSVERKSTDENYLAAHPPERSDASTTNAFASPVLWLSGGFLVLFV
CCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCHHHHHCCHHHHCCCHHHHHH
LLAVFDTSALSSVIQVGFSKVTLWFGPFWQVLLLANFVIGLYLAFARTGDVTLGNLKAPE
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCC
MDTFKWLAIILCTLLAGGGVFWAAAEPIAHFVSSPPFYGEAEGRRLAINALSQSFLHWGF
CHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHH
LAWAILGSLAAIVLMHLHYDKGLPLQPRTLLYPIFGDRALNSWVGTLTDTFCIIAVAAGT
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCC
IGPIGFLGLQISYALNELFGVQDTLITQSLVVVFAIAMYTLSALSGLSRGIQLVSRYNIL
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSVALIIFILLFGPTSFIVDGYMQGVARMVNDFVPMSLYRGDANWLSWWTVFFWGWFIGY
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHH
GPMMAIFIARISRGRTIRQLILSISIIAPLITCFWFSVVGGSGLAFELANPGVIANAFEG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEHHHCC
FNLPGVLLAITSQLPMPTLIAILFLILTTTFIVTTGDSMTYTISVVVTGSTEPSGAMRAF
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHEECCCCEEEEEEEEEECCCCCCHHHHHH
WGILMGLVAIALIAMGSGGISALQSFIVITAVPVSFVLLPTLWQAPKMAKAMAEQQFGKA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHH
SLNQQAERS
HHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]