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The map label for this gene is arnT [H]

Identifier: 157963440

GI number: 157963440

Start: 4416924

End: 4418363

Strand: Reverse

Name: arnT [H]

Synonym: Spea_3628

Alternate gene names: 157963440

Gene position: 4418363-4416924 (Counterclockwise)

Preceding gene: 157963441

Following gene: 157963439

Centisome position: 85.39

GC content: 50.9

Gene sequence:

>1440_bases
ATGTTGAGTATGCGCGATAGTCAAAAGGGGTTAGCGGTACTCTTGATTGTGGTTCTCACTCGGTTATTACTATTGCCTTG
GTATCCACTTATGGACACCACCGAGGCGCGATACGGCGAGATGGCGCGCCTGATGGTAGAGACGGGAAATTGGGTCACGC
CACTGTTTGATTACAATGTCCCGTTTTGGGGCAAGCCTCCCCTGCATACTTGGATGAGTGCGAGCTCTTTAGCTGTATTG
GGGAGCAATGAGTTTGCCGTGCGCTTGCCTCACTGGCTGGCAGCGATGTTTACCCTCGCGGTTGTGGGTTACTTTGCTAA
GGGTCTACGTTTACCTGTGATGTCGGTGTTAATCATCTTAGCCTCTACAGTGGTGTTTACTATCAGTGCTGGCGCGGTAA
TGACAGATATGGCGCTGACACTTGGGATGACGCTGGCAATGGTGGGTTTTTATCTTTGCTGGCAAGGTGACAAGCTCTGG
GGTTATATCGGCTTTGCCGGACTGGCGATAGGCCTGCTGGCTAAAGGCCCTGTGGCATTAGTCTTGTTCGCCATAGGCAC
TGGGCTGTGGTTGATGTGGAACTATGGCTTGCAAAAGATGTGGCGGCTTTTATGGCTGCGTACGCCCCTGCTGACGGGGA
CGTTACTGATGCTAGCGATTACACTGCCTTGGTATGTGATGGCGGAGCGGGCGACCCCGGGTTTTTTAAATTACTTTATT
GTGGGTGAGCATTGGTCTCGCTTCGTTGATAGTGGCTGGCAAGGGGATCTCTATGGCTCGGCACATGACGAGATAAGAGG
CAGTATCTGGCTGTATTTTATTGGCGCAGCGATGCCTTGGTCACTGTTTCTGCCTAGAGCGATTTACAAGTTAGTTAAGC
AGTCTAAAAGCGCGGCGACAGCGGTTGAGGCTGGGGTAGGTAAAGAGACAGGGACAGAAACGGGGTCAGAGACTGGGGAA
GGTCTAGCTAGCTATCTTTTTTGCTGGATGATTGCCCCTATGCTGCTGTTTACGCTAGCGGGGAATATTCTTCCTGCCTA
TGTTCTACCGGGGATCCCAGGTTTAGCCTTACTGCTGGGGTTAGCCTGGAGGAGTAGTCGCTTACCTGGGCTAAATGCTA
TCGCCTTATGCATACCTGTGCTGCTATTGGTTACAGTTGTTGTACTGAACTTAGGTACGGCGAAAGATAAGAGTGAGCGC
TGGTTATTGTCACAGCGCAGTGAAGCCTTACCGACCTATTACTGGCAGAAGCAACCGTTTTCGGCGCGTTTCTATAGCGC
AGGCCAAGCTGACAAGGTAGATAGTTTGCAGGAGGTGGACTCAATAAGTGATTTTTACTTAGTGGCCGATAATCGCAAAC
TAGATCTGCCGCAGTTTTCTCGCTGTGTTGAGGTGGCGCGCAATAAGTATCGAGGGCTACTGCTTTGTCGTCGAAATTAA

Upstream 100 bases:

>100_bases
ACTTAATTGAAGAGGTCGCCTATCGACACTCATTAGCTCAAGTACAAGCCAGAAATAGCGATATAAAACAGGACAACGTA
AGTTACTTGGGGGCTAAATA

Downstream 100 bases:

>100_bases
CTACAGTCCCAGTGCCTGAACTAGAGACGTTATGGTTAAAGCATGGCCAAGGCATTCGCTTTCTGCTGGTGGGGAGCGCG
GTTTTCATGTTCGATGCTGC

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: 4-amino-4-deoxy-L-arabinose lipid A transferase; Lipid IV(A) 4-amino-4-deoxy-L-arabinosyltransferase; Undecaprenyl phosphate-alpha-L-Ara4N transferase [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MLSMRDSQKGLAVLLIVVLTRLLLLPWYPLMDTTEARYGEMARLMVETGNWVTPLFDYNVPFWGKPPLHTWMSASSLAVL
GSNEFAVRLPHWLAAMFTLAVVGYFAKGLRLPVMSVLIILASTVVFTISAGAVMTDMALTLGMTLAMVGFYLCWQGDKLW
GYIGFAGLAIGLLAKGPVALVLFAIGTGLWLMWNYGLQKMWRLLWLRTPLLTGTLLMLAITLPWYVMAERATPGFLNYFI
VGEHWSRFVDSGWQGDLYGSAHDEIRGSIWLYFIGAAMPWSLFLPRAIYKLVKQSKSAATAVEAGVGKETGTETGSETGE
GLASYLFCWMIAPMLLFTLAGNILPAYVLPGIPGLALLLGLAWRSSRLPGLNAIALCIPVLLLVTVVVLNLGTAKDKSER
WLLSQRSEALPTYYWQKQPFSARFYSAGQADKVDSLQEVDSISDFYLVADNRKLDLPQFSRCVEVARNKYRGLLLCRRN

Sequences:

>Translated_479_residues
MLSMRDSQKGLAVLLIVVLTRLLLLPWYPLMDTTEARYGEMARLMVETGNWVTPLFDYNVPFWGKPPLHTWMSASSLAVL
GSNEFAVRLPHWLAAMFTLAVVGYFAKGLRLPVMSVLIILASTVVFTISAGAVMTDMALTLGMTLAMVGFYLCWQGDKLW
GYIGFAGLAIGLLAKGPVALVLFAIGTGLWLMWNYGLQKMWRLLWLRTPLLTGTLLMLAITLPWYVMAERATPGFLNYFI
VGEHWSRFVDSGWQGDLYGSAHDEIRGSIWLYFIGAAMPWSLFLPRAIYKLVKQSKSAATAVEAGVGKETGTETGSETGE
GLASYLFCWMIAPMLLFTLAGNILPAYVLPGIPGLALLLGLAWRSSRLPGLNAIALCIPVLLLVTVVVLNLGTAKDKSER
WLLSQRSEALPTYYWQKQPFSARFYSAGQADKVDSLQEVDSISDFYLVADNRKLDLPQFSRCVEVARNKYRGLLLCRRN
>Mature_479_residues
MLSMRDSQKGLAVLLIVVLTRLLLLPWYPLMDTTEARYGEMARLMVETGNWVTPLFDYNVPFWGKPPLHTWMSASSLAVL
GSNEFAVRLPHWLAAMFTLAVVGYFAKGLRLPVMSVLIILASTVVFTISAGAVMTDMALTLGMTLAMVGFYLCWQGDKLW
GYIGFAGLAIGLLAKGPVALVLFAIGTGLWLMWNYGLQKMWRLLWLRTPLLTGTLLMLAITLPWYVMAERATPGFLNYFI
VGEHWSRFVDSGWQGDLYGSAHDEIRGSIWLYFIGAAMPWSLFLPRAIYKLVKQSKSAATAVEAGVGKETGTETGSETGE
GLASYLFCWMIAPMLLFTLAGNILPAYVLPGIPGLALLLGLAWRSSRLPGLNAIALCIPVLLLVTVVVLNLGTAKDKSER
WLLSQRSEALPTYYWQKQPFSARFYSAGQADKVDSLQEVDSISDFYLVADNRKLDLPQFSRCVEVARNKYRGLLLCRRN

Specific function: Catalyzes the transfer of the L-Ara4N moiety of the glycolipid undecaprenyl phosphate-alpha-L-Ara4N to lipid A. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides [H]

COG id: COG1807

COG function: function code M; 4-amino-4-deoxy-L-arabinose transferase and related glycosyltransferases of PMT family

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 83 family [H]

Homologues:

Organism=Escherichia coli, GI1788591, Length=358, Percent_Identity=27.9329608938547, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022839
- InterPro:   IPR003342 [H]

Pfam domain/function: PF02366 PMT [H]

EC number: =2.4.2.43 [H]

Molecular weight: Translated: 53214; Mature: 53214

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS00152 ATPASE_ALPHA_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSMRDSQKGLAVLLIVVLTRLLLLPWYPLMDTTEARYGEMARLMVETGNWVTPLFDYNV
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCC
PFWGKPPLHTWMSASSLAVLGSNEFAVRLPHWLAAMFTLAVVGYFAKGLRLPVMSVLIIL
CCCCCCCHHHHHCCCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ASTVVFTISAGAVMTDMALTLGMTLAMVGFYLCWQGDKLWGYIGFAGLAIGLLAKGPVAL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHH
VLFAIGTGLWLMWNYGLQKMWRLLWLRTPLLTGTLLMLAITLPWYVMAERATPGFLNYFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
VGEHWSRFVDSGWQGDLYGSAHDEIRGSIWLYFIGAAMPWSLFLPRAIYKLVKQSKSAAT
ECHHHHHHHCCCCCCCCCCCCHHHHCCCEEEEEHHHHCCHHHHHHHHHHHHHHHCCHHHH
AVEAGVGKETGTETGSETGEGLASYLFCWMIAPMLLFTLAGNILPAYVLPGIPGLALLLG
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LAWRSSRLPGLNAIALCIPVLLLVTVVVLNLGTAKDKSERWLLSQRSEALPTYYWQKQPF
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHCCCCCC
SARFYSAGQADKVDSLQEVDSISDFYLVADNRKLDLPQFSRCVEVARNKYRGLLLCRRN
CCHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEECC
>Mature Secondary Structure
MLSMRDSQKGLAVLLIVVLTRLLLLPWYPLMDTTEARYGEMARLMVETGNWVTPLFDYNV
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCC
PFWGKPPLHTWMSASSLAVLGSNEFAVRLPHWLAAMFTLAVVGYFAKGLRLPVMSVLIIL
CCCCCCCHHHHHCCCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ASTVVFTISAGAVMTDMALTLGMTLAMVGFYLCWQGDKLWGYIGFAGLAIGLLAKGPVAL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHH
VLFAIGTGLWLMWNYGLQKMWRLLWLRTPLLTGTLLMLAITLPWYVMAERATPGFLNYFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
VGEHWSRFVDSGWQGDLYGSAHDEIRGSIWLYFIGAAMPWSLFLPRAIYKLVKQSKSAAT
ECHHHHHHHCCCCCCCCCCCCHHHHCCCEEEEEHHHHCCHHHHHHHHHHHHHHHCCHHHH
AVEAGVGKETGTETGSETGEGLASYLFCWMIAPMLLFTLAGNILPAYVLPGIPGLALLLG
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LAWRSSRLPGLNAIALCIPVLLLVTVVVLNLGTAKDKSERWLLSQRSEALPTYYWQKQPF
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHCCCCCC
SARFYSAGQADKVDSLQEVDSISDFYLVADNRKLDLPQFSRCVEVARNKYRGLLLCRRN
CCHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA