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The map label for this gene is purA [H]

Identifier: 157963406

GI number: 157963406

Start: 4385473

End: 4386768

Strand: Reverse

Name: purA [H]

Synonym: Spea_3592

Alternate gene names: 157963406

Gene position: 4386768-4385473 (Counterclockwise)

Preceding gene: 157963407

Following gene: 157963405

Centisome position: 84.78

GC content: 45.52

Gene sequence:

>1296_bases
ATGGGCAAAAACGTTGTAGTTCTCGGCACCCAATGGGGTGACGAAGGAAAGGGTAAGATAGTCGATCTTCTTACCGAACA
GGCTAAATATGTCGTTCGTTACCAAGGTGGCCACAATGCGGGTCATACTCTAGTAATTGATGGCGACAAAACCGTTCTTC
ATCTTATTCCGTCAGGGATCTTACGCGATAATGTTAAGTGCATTATTGGTAACGGCGTGGTGCTAGCACCTGACGCGCTG
ATGACAGAAATTAACATGCTTAAAGAGCGTGGCGTACCTGTAGAGGAACGTTTATTAATTTCTGAAGCATGTCCTCTAAT
TCTACCATTCCACTGTGCCCTTGATATTGCTCGCGAAAAAGCGCGCGGTAACAATGCAATTGGTACAACGGGTCGTGGTA
TTGGTCCAGCATACGAAGATAAAGTTTCTCGTCGCGGTTTACGTGTAGGCGATCTGTTTGATGCAGAATTGTTTGCCGTT
AAGCTGAAAGAAGTGATGGCTTACCATAACTTCATGCTGACTGAATATTACAAGTGTGAAGCTGTTGATTACGAAGAGAC
ATTGAAAGATGCTCTAGCAATTGCTGATTACTTGAAGAGCATGTGCACTGACGTTTCTGAACTTCTTGATCAGGCGCGTA
AAGCAGGTGAACCAATTCTATTTGAAGGTGCTCAGGGCACGTTACTAGATATTGACCATGGTACTTATCCGTTCGTAACC
TCTTCTAATACTACCGCAGGTGGTGTTGCAACAGGTTCAGGATTTGGCCCACGTCATCTAGACTACGTTTTGGGTATTAT
GAAGGCATACACCACACGTGTTGGTGCTGGTCCTTTCCCTACAGAATTGAAAAACGAAATCGGCGATTACTTAGGTACTA
AGGGTCACGAATTCGGCGCAACTACTGGTCGTAAGCGTCGTCCAGGTTGGTTAGATATCGTCGCTATGAAGCGCGCGGTT
CAAATCAACAGTGTTAGCGGTTTCTGCTTAACTAAGCTAGACGTTCTAGACGGCCTAGAAGAAGTTAAGATCTGTGTTGG
CTACCAGTATCCTGATGGAACTGTAGCGACAACGACTCCTCTAGCGGCTGAAGGTTACGAGAAGGTTACCCCTGTTCTTG
AAACTATGCCTGGTTGGAGCGAGTCAACTTTCGGCGCAACTTCTGTTGAGCAATTGCCACAAGCGGCATTGAACTACATT
AAGCGTCTTGAAGAGTTGCTAGAAACGCCAATCGATATTATCTCAACGGGTCCGGATAGAAACGAGACCATGATTCTAGT
GAATCCGTTCAGTTAA

Upstream 100 bases:

>100_bases
CGTAGGCATAGTGCTGTTGATTATTTTTTCATAAATTACTTGCCTATAGCCCCCATAGATCTGCTAAAATCCCGTTTTAA
CCACTACCGTGCAGCAAATA

Downstream 100 bases:

>100_bases
TAGAAAAGGCCGCAATTAGCGGCCTTTTTATTGCCTTGCTTGCAAGTGACGACATCGCGACTGTAGCACTCAAGAAATAC
GTTATACTGCCGATGACATT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MGKNVVVLGTQWGDEGKGKIVDLLTEQAKYVVRYQGGHNAGHTLVIDGDKTVLHLIPSGILRDNVKCIIGNGVVLAPDAL
MTEINMLKERGVPVEERLLISEACPLILPFHCALDIAREKARGNNAIGTTGRGIGPAYEDKVSRRGLRVGDLFDAELFAV
KLKEVMAYHNFMLTEYYKCEAVDYEETLKDALAIADYLKSMCTDVSELLDQARKAGEPILFEGAQGTLLDIDHGTYPFVT
SSNTTAGGVATGSGFGPRHLDYVLGIMKAYTTRVGAGPFPTELKNEIGDYLGTKGHEFGATTGRKRRPGWLDIVAMKRAV
QINSVSGFCLTKLDVLDGLEEVKICVGYQYPDGTVATTTPLAAEGYEKVTPVLETMPGWSESTFGATSVEQLPQAALNYI
KRLEELLETPIDIISTGPDRNETMILVNPFS

Sequences:

>Translated_431_residues
MGKNVVVLGTQWGDEGKGKIVDLLTEQAKYVVRYQGGHNAGHTLVIDGDKTVLHLIPSGILRDNVKCIIGNGVVLAPDAL
MTEINMLKERGVPVEERLLISEACPLILPFHCALDIAREKARGNNAIGTTGRGIGPAYEDKVSRRGLRVGDLFDAELFAV
KLKEVMAYHNFMLTEYYKCEAVDYEETLKDALAIADYLKSMCTDVSELLDQARKAGEPILFEGAQGTLLDIDHGTYPFVT
SSNTTAGGVATGSGFGPRHLDYVLGIMKAYTTRVGAGPFPTELKNEIGDYLGTKGHEFGATTGRKRRPGWLDIVAMKRAV
QINSVSGFCLTKLDVLDGLEEVKICVGYQYPDGTVATTTPLAAEGYEKVTPVLETMPGWSESTFGATSVEQLPQAALNYI
KRLEELLETPIDIISTGPDRNETMILVNPFS
>Mature_430_residues
GKNVVVLGTQWGDEGKGKIVDLLTEQAKYVVRYQGGHNAGHTLVIDGDKTVLHLIPSGILRDNVKCIIGNGVVLAPDALM
TEINMLKERGVPVEERLLISEACPLILPFHCALDIAREKARGNNAIGTTGRGIGPAYEDKVSRRGLRVGDLFDAELFAVK
LKEVMAYHNFMLTEYYKCEAVDYEETLKDALAIADYLKSMCTDVSELLDQARKAGEPILFEGAQGTLLDIDHGTYPFVTS
SNTTAGGVATGSGFGPRHLDYVLGIMKAYTTRVGAGPFPTELKNEIGDYLGTKGHEFGATTGRKRRPGWLDIVAMKRAVQ
INSVSGFCLTKLDVLDGLEEVKICVGYQYPDGTVATTTPLAAEGYEKVTPVLETMPGWSESTFGATSVEQLPQAALNYIK
RLEELLETPIDIISTGPDRNETMILVNPFS

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=428, Percent_Identity=45.3271028037383, Blast_Score=331, Evalue=7e-91,
Organism=Homo sapiens, GI34577063, Length=429, Percent_Identity=45.4545454545455, Blast_Score=331, Evalue=7e-91,
Organism=Homo sapiens, GI40316944, Length=399, Percent_Identity=43.609022556391, Blast_Score=291, Evalue=7e-79,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=76.2790697674419, Blast_Score=683, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25146555, Length=434, Percent_Identity=41.9354838709677, Blast_Score=318, Evalue=4e-87,
Organism=Caenorhabditis elegans, GI25146553, Length=434, Percent_Identity=41.9354838709677, Blast_Score=318, Evalue=5e-87,
Organism=Saccharomyces cerevisiae, GI6324109, Length=429, Percent_Identity=42.1911421911422, Blast_Score=320, Evalue=4e-88,
Organism=Drosophila melanogaster, GI21356033, Length=425, Percent_Identity=43.0588235294118, Blast_Score=335, Evalue=5e-92,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46836; Mature: 46705

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKNVVVLGTQWGDEGKGKIVDLLTEQAKYVVRYQGGHNAGHTLVIDGDKTVLHLIPSGI
CCCCEEEEEECCCCCCCCHHHHHHHHCCEEEEEECCCCCCCCEEEECCCCEEEEECCCHH
LRDNVKCIIGNGVVLAPDALMTEINMLKERGVPVEERLLISEACPLILPFHCALDIAREK
HHCCCEEEEECCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHH
ARGNNAIGTTGRGIGPAYEDKVSRRGLRVGDLFDAELFAVKLKEVMAYHNFMLTEYYKCE
HCCCCCCCCCCCCCCCHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHEEEEC
AVDYEETLKDALAIADYLKSMCTDVSELLDQARKAGEPILFEGAQGTLLDIDHGTYPFVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVATGSGFGPRHLDYVLGIMKAYTTRVGAGPFPTELKNEIGDYLGTKGHEFGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCC
TTGRKRRPGWLDIVAMKRAVQINSVSGFCLTKLDVLDGLEEVKICVGYQYPDGTVATTTP
CCCCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHCCHHHEEEEEEEECCCCCEEECCC
LAAEGYEKVTPVLETMPGWSESTFGATSVEQLPQAALNYIKRLEELLETPIDIISTGPDR
CCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCC
NETMILVNPFS
CCEEEEEECCC
>Mature Secondary Structure 
GKNVVVLGTQWGDEGKGKIVDLLTEQAKYVVRYQGGHNAGHTLVIDGDKTVLHLIPSGI
CCCEEEEEECCCCCCCCHHHHHHHHCCEEEEEECCCCCCCCEEEECCCCEEEEECCCHH
LRDNVKCIIGNGVVLAPDALMTEINMLKERGVPVEERLLISEACPLILPFHCALDIAREK
HHCCCEEEEECCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHH
ARGNNAIGTTGRGIGPAYEDKVSRRGLRVGDLFDAELFAVKLKEVMAYHNFMLTEYYKCE
HCCCCCCCCCCCCCCCHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHEEEEC
AVDYEETLKDALAIADYLKSMCTDVSELLDQARKAGEPILFEGAQGTLLDIDHGTYPFVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVATGSGFGPRHLDYVLGIMKAYTTRVGAGPFPTELKNEIGDYLGTKGHEFGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCC
TTGRKRRPGWLDIVAMKRAVQINSVSGFCLTKLDVLDGLEEVKICVGYQYPDGTVATTTP
CCCCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHCCHHHEEEEEEEECCCCCEEECCC
LAAEGYEKVTPVLETMPGWSESTFGATSVEQLPQAALNYIKRLEELLETPIDIISTGPDR
CCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCC
NETMILVNPFS
CCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA