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The map label for this gene is ptrB [C]

Identifier: 157963402

GI number: 157963402

Start: 4379122

End: 4381209

Strand: Reverse

Name: ptrB [C]

Synonym: Spea_3588

Alternate gene names: 157963402

Gene position: 4381209-4379122 (Counterclockwise)

Preceding gene: 157963403

Following gene: 157963400

Centisome position: 84.67

GC content: 45.79

Gene sequence:

>2088_bases
ATGCGAAATCAAATTTTATCTTTGTGCGTACTGGGTGCAATTGCTGCCGTACCCGCTCACGCAGAGGATGATAACTACCT
CTGGCTAGAAGATGTTGAAGGCGCAAAGCCGATGGAATGGGTGAAACAGCAAAATGCTATTTCGGCCAAAGAGATTAAGG
CATTCGAAGGCTTCGATAACTTAGTGAGTAATAGCCTGGCCATCCTAAACGACAAAGAGCGCATCCCATACGCCAGTCGT
ATTGGCGATTACCTATATAACTTCTGGAAAGACGAGAACCACGTTAGAGGGATCTACCGCAGAACTACCATGGAGGAGTA
TAAGAAGACTTCGCCTAAATGGGAAACGGTACTCGATATCGACAAGCTAGGCAAAGATGAGGGGGTTAACTGGGTCTATA
AGGGCTTTAACTGTCGTTACCCTGAAAATGATCGTTGCTTTGTCTCTCTTTCACGTGGGGGCGCCGATGCAACTGAGGTG
CGAGAGTTTGATCTCAATACGCTCTCCTTTGTGGGGGAAAAACAACAAGCATTCTTTCTCGAAGAAGCTAAGTCGAGCCT
GAGCTGGATTAATAAGAATAACGTCTTTGTTGGCACCGACTTTGGTGACGATAGCATGACGGATTCTGGTTACCCTAAGG
TGGTTAAGCTTTGGGAGCGTGGTACTAAGCTTTCTGAAGCAACAACGGTTTTCAGTGGTGATAAAGCGTCGGTAGCGGTA
TCAGGCTATGTACTGTTTGACGGCGATAATTCCTTGGAGCTAGTGTCAGAGGCGTTGACCTTCTATACCGCCGAGCATTA
TGTTTACCGCGATGGAAAACTCACCTTATTGCCTCTACCAAAAGATGCTGAACTTAAAGGCTATTTTAACGGCCAGCTGT
ATATCGAGCTAAAAAGCGATCTTAAAACAGGGAGCGAGAGCTTCAAGCAAGGTAGCATTATCTATGCTCCGGTAGAGACG
CTTATTGAGCAGAAGCCACAATTTAACGTGTTAGTGGAGCCATCGGAAACCGCTTCTATCTCACAAGTTAAATTTACCAA
GAGTGCGATTTTCGTTACTTGGTTAGATAACGTCAAAGGCAAATTGGTACGTTACTCTCAAGCAAAAAACGGTCAGTGGA
AAAGCATGCAAGCGCCGTTCGATGCTAATGGCACCATTAGCGTGTTTGATGTCGAGCGTGATAGTGATGACTTCTTCGTT
AACTATACTAGCTTCTTAGAGCCTTCAAGTTTATATAGCTTTAATGCTGTGACCATGAAAGCCGAAAAGATTAAGGGCAT
GAAACAGCAGTTCGCTGCCTCAAAGTTTGAAACTAAACAATATTTCGCTACCTCAAAAGATGGTACTCAGGTGCCCTATT
TTGTGGTGATGGCGAAAGATCTTAAACGTGATGGCAAAAATCCGACTCTGCTTTATGGATATGGTGGTTTTGAAGTTTCT
CTGCGTCCCTCTTATTCGGCAACAATCGGTAAAAATTGGTTGGAGCAAGGCGGTGTTTATGTGCTAGCCAACATTCGTGG
TGGAGGCGAATATGGCCCAGCTTGGCATCAAGCGGCTCTGAAACAGAATCGTCATAAAGCCTATGAAGATTTTGAGGCAA
TTGCAGAGGACTTGATAGCTAAGAAGATTACCTCGAGTGAGCATCTAGGGATCCAAGGTGGCAGTAATGGTGGCTTGCTG
ATGGGGGCTGCATTTACTCGTAAACCTGAGCTTTATAGTGCAGTTGTATGCCAGGTACCGTTACTGGATATGAAGCGCTA
CAACAAGCTGTTGGCTGGAGCGAGCTGGATGGGGGAGTACGGCGATCCGGATATTCCTGCCCAGTGGGATTATATTAAGA
CATTTTCGCCTTACCATAACCTAAAGGCGAATACAGCTTACCCGAAGGTGTTTTTCACCACCTCAACCCGTGATGACAGG
GTTCATCCCGCTCATGCTCGCAAGATGGTGGCTAAGATGGAAGGCATGGGCATTGATGTCTTGTATTATGAGAATATCGA
AGGGGGTCACGCTGGCGCTGCTGATAATAATCAGGCGGCGGAGCTAAAGTCGATGGTATACGCTTATCTTATGCAACAAC
TAAGGTAA

Upstream 100 bases:

>100_bases
CTTTGTTACACCATAGCCAGTACTGTATCGATAGGCTCTATTTAATTGTTCCGCATTATGCAGTAGCTTAAGCTGACAAA
TTCTAAAACAGGATGTTTAC

Downstream 100 bases:

>100_bases
GTTGTAATTCGGTCCATTAATTGTGAAGGGCAAACTCGACAGAGCTTGCCCTTTATTGTTTGTGACGTTAGCGGTTATTG
AAAGGTATAGAGCAAGTTGA

Product: prolyl oligopeptidase

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 695; Mature: 695

Protein sequence:

>695_residues
MRNQILSLCVLGAIAAVPAHAEDDNYLWLEDVEGAKPMEWVKQQNAISAKEIKAFEGFDNLVSNSLAILNDKERIPYASR
IGDYLYNFWKDENHVRGIYRRTTMEEYKKTSPKWETVLDIDKLGKDEGVNWVYKGFNCRYPENDRCFVSLSRGGADATEV
REFDLNTLSFVGEKQQAFFLEEAKSSLSWINKNNVFVGTDFGDDSMTDSGYPKVVKLWERGTKLSEATTVFSGDKASVAV
SGYVLFDGDNSLELVSEALTFYTAEHYVYRDGKLTLLPLPKDAELKGYFNGQLYIELKSDLKTGSESFKQGSIIYAPVET
LIEQKPQFNVLVEPSETASISQVKFTKSAIFVTWLDNVKGKLVRYSQAKNGQWKSMQAPFDANGTISVFDVERDSDDFFV
NYTSFLEPSSLYSFNAVTMKAEKIKGMKQQFAASKFETKQYFATSKDGTQVPYFVVMAKDLKRDGKNPTLLYGYGGFEVS
LRPSYSATIGKNWLEQGGVYVLANIRGGGEYGPAWHQAALKQNRHKAYEDFEAIAEDLIAKKITSSEHLGIQGGSNGGLL
MGAAFTRKPELYSAVVCQVPLLDMKRYNKLLAGASWMGEYGDPDIPAQWDYIKTFSPYHNLKANTAYPKVFFTTSTRDDR
VHPAHARKMVAKMEGMGIDVLYYENIEGGHAGAADNNQAAELKSMVYAYLMQQLR

Sequences:

>Translated_695_residues
MRNQILSLCVLGAIAAVPAHAEDDNYLWLEDVEGAKPMEWVKQQNAISAKEIKAFEGFDNLVSNSLAILNDKERIPYASR
IGDYLYNFWKDENHVRGIYRRTTMEEYKKTSPKWETVLDIDKLGKDEGVNWVYKGFNCRYPENDRCFVSLSRGGADATEV
REFDLNTLSFVGEKQQAFFLEEAKSSLSWINKNNVFVGTDFGDDSMTDSGYPKVVKLWERGTKLSEATTVFSGDKASVAV
SGYVLFDGDNSLELVSEALTFYTAEHYVYRDGKLTLLPLPKDAELKGYFNGQLYIELKSDLKTGSESFKQGSIIYAPVET
LIEQKPQFNVLVEPSETASISQVKFTKSAIFVTWLDNVKGKLVRYSQAKNGQWKSMQAPFDANGTISVFDVERDSDDFFV
NYTSFLEPSSLYSFNAVTMKAEKIKGMKQQFAASKFETKQYFATSKDGTQVPYFVVMAKDLKRDGKNPTLLYGYGGFEVS
LRPSYSATIGKNWLEQGGVYVLANIRGGGEYGPAWHQAALKQNRHKAYEDFEAIAEDLIAKKITSSEHLGIQGGSNGGLL
MGAAFTRKPELYSAVVCQVPLLDMKRYNKLLAGASWMGEYGDPDIPAQWDYIKTFSPYHNLKANTAYPKVFFTTSTRDDR
VHPAHARKMVAKMEGMGIDVLYYENIEGGHAGAADNNQAAELKSMVYAYLMQQLR
>Mature_695_residues
MRNQILSLCVLGAIAAVPAHAEDDNYLWLEDVEGAKPMEWVKQQNAISAKEIKAFEGFDNLVSNSLAILNDKERIPYASR
IGDYLYNFWKDENHVRGIYRRTTMEEYKKTSPKWETVLDIDKLGKDEGVNWVYKGFNCRYPENDRCFVSLSRGGADATEV
REFDLNTLSFVGEKQQAFFLEEAKSSLSWINKNNVFVGTDFGDDSMTDSGYPKVVKLWERGTKLSEATTVFSGDKASVAV
SGYVLFDGDNSLELVSEALTFYTAEHYVYRDGKLTLLPLPKDAELKGYFNGQLYIELKSDLKTGSESFKQGSIIYAPVET
LIEQKPQFNVLVEPSETASISQVKFTKSAIFVTWLDNVKGKLVRYSQAKNGQWKSMQAPFDANGTISVFDVERDSDDFFV
NYTSFLEPSSLYSFNAVTMKAEKIKGMKQQFAASKFETKQYFATSKDGTQVPYFVVMAKDLKRDGKNPTLLYGYGGFEVS
LRPSYSATIGKNWLEQGGVYVLANIRGGGEYGPAWHQAALKQNRHKAYEDFEAIAEDLIAKKITSSEHLGIQGGSNGGLL
MGAAFTRKPELYSAVVCQVPLLDMKRYNKLLAGASWMGEYGDPDIPAQWDYIKTFSPYHNLKANTAYPKVFFTTSTRDDR
VHPAHARKMVAKMEGMGIDVLYYENIEGGHAGAADNNQAAELKSMVYAYLMQQLR

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=390, Percent_Identity=32.0512820512821, Blast_Score=219, Evalue=6e-57,
Organism=Homo sapiens, GI284172438, Length=274, Percent_Identity=25.9124087591241, Blast_Score=86, Evalue=2e-16,
Organism=Homo sapiens, GI284172431, Length=274, Percent_Identity=25.9124087591241, Blast_Score=86, Evalue=2e-16,
Organism=Homo sapiens, GI284172420, Length=278, Percent_Identity=25.8992805755396, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI284172413, Length=278, Percent_Identity=25.8992805755396, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI70778815, Length=278, Percent_Identity=25.8992805755396, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI108860686, Length=264, Percent_Identity=25.3787878787879, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI108860692, Length=258, Percent_Identity=25.5813953488372, Blast_Score=83, Evalue=9e-16,
Organism=Escherichia coli, GI1788150, Length=696, Percent_Identity=22.8448275862069, Blast_Score=151, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24583414, Length=720, Percent_Identity=28.6111111111111, Blast_Score=221, Evalue=2e-57,
Organism=Drosophila melanogaster, GI221510989, Length=679, Percent_Identity=26.9513991163476, Blast_Score=184, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 78081; Mature: 78081

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNQILSLCVLGAIAAVPAHAEDDNYLWLEDVEGAKPMEWVKQQNAISAKEIKAFEGFDN
CCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHH
LVSNSLAILNDKERIPYASRIGDYLYNFWKDENHVRGIYRRTTMEEYKKTSPKWETVLDI
HHHCCEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHEEEEH
DKLGKDEGVNWVYKGFNCRYPENDRCFVSLSRGGADATEVREFDLNTLSFVGEKQQAFFL
HHCCCCCCCCEEEECEECCCCCCCCEEEEEECCCCCHHHHHHCCCCHHHHCCCHHHHHHH
EEAKSSLSWINKNNVFVGTDFGDDSMTDSGYPKVVKLWERGTKLSEATTVFSGDKASVAV
HHHHHHHHEECCCCEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCHHEEEECCCCCEEEE
SGYVLFDGDNSLELVSEALTFYTAEHYVYRDGKLTLLPLPKDAELKGYFNGQLYIELKSD
EEEEEECCCCHHHHHHHHHHHHEECEEEEECCCEEEEECCCCCCCEEEECCEEEEEECCC
LKTGSESFKQGSIIYAPVETLIEQKPQFNVLVEPSETASISQVKFTKSAIFVTWLDNVKG
CCCCHHHHHCCCEEEECHHHHHCCCCCCEEEECCCCCCCCEEEEEECCEEEEEEECCCCC
KLVRYSQAKNGQWKSMQAPFDANGTISVFDVERDSDDFFVNYTSFLEPSSLYSFNAVTMK
EEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEHHHCCCCCCEEEEEEEEE
AEKIKGMKQQFAASKFETKQYFATSKDGTQVPYFVVMAKDLKRDGKNPTLLYGYGGFEVS
HHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCEEEEEEHHHHHCCCCCEEEEEECCEEEE
LRPSYSATIGKNWLEQGGVYVLANIRGGGEYGPAWHQAALKQNRHKAYEDFEAIAEDLIA
ECCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKITSSEHLGIQGGSNGGLLMGAAFTRKPELYSAVVCQVPLLDMKRYNKLLAGASWMGEY
HHCCCCCCCEEECCCCCCEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCHHCCCC
GDPDIPAQWDYIKTFSPYHNLKANTAYPKVFFTTSTRDDRVHPAHARKMVAKMEGMGIDV
CCCCCCCCCCHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEE
LYYENIEGGHAGAADNNQAAELKSMVYAYLMQQLR
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRNQILSLCVLGAIAAVPAHAEDDNYLWLEDVEGAKPMEWVKQQNAISAKEIKAFEGFDN
CCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHH
LVSNSLAILNDKERIPYASRIGDYLYNFWKDENHVRGIYRRTTMEEYKKTSPKWETVLDI
HHHCCEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHEEEEH
DKLGKDEGVNWVYKGFNCRYPENDRCFVSLSRGGADATEVREFDLNTLSFVGEKQQAFFL
HHCCCCCCCCEEEECEECCCCCCCCEEEEEECCCCCHHHHHHCCCCHHHHCCCHHHHHHH
EEAKSSLSWINKNNVFVGTDFGDDSMTDSGYPKVVKLWERGTKLSEATTVFSGDKASVAV
HHHHHHHHEECCCCEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCHHEEEECCCCCEEEE
SGYVLFDGDNSLELVSEALTFYTAEHYVYRDGKLTLLPLPKDAELKGYFNGQLYIELKSD
EEEEEECCCCHHHHHHHHHHHHEECEEEEECCCEEEEECCCCCCCEEEECCEEEEEECCC
LKTGSESFKQGSIIYAPVETLIEQKPQFNVLVEPSETASISQVKFTKSAIFVTWLDNVKG
CCCCHHHHHCCCEEEECHHHHHCCCCCCEEEECCCCCCCCEEEEEECCEEEEEEECCCCC
KLVRYSQAKNGQWKSMQAPFDANGTISVFDVERDSDDFFVNYTSFLEPSSLYSFNAVTMK
EEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEHHHCCCCCCEEEEEEEEE
AEKIKGMKQQFAASKFETKQYFATSKDGTQVPYFVVMAKDLKRDGKNPTLLYGYGGFEVS
HHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCEEEEEEHHHHHCCCCCEEEEEECCEEEE
LRPSYSATIGKNWLEQGGVYVLANIRGGGEYGPAWHQAALKQNRHKAYEDFEAIAEDLIA
ECCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKITSSEHLGIQGGSNGGLLMGAAFTRKPELYSAVVCQVPLLDMKRYNKLLAGASWMGEY
HHCCCCCCCEEECCCCCCEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCHHCCCC
GDPDIPAQWDYIKTFSPYHNLKANTAYPKVFFTTSTRDDRVHPAHARKMVAKMEGMGIDV
CCCCCCCCCCHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEE
LYYENIEGGHAGAADNNQAAELKSMVYAYLMQQLR
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} 0.47 {tosyl-Lys-methyl} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]