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The map label for this gene is cobQ [H]

Identifier: 157963192

GI number: 157963192

Start: 4124766

End: 4126376

Strand: Reverse

Name: cobQ [H]

Synonym: Spea_3377

Alternate gene names: 157963192

Gene position: 4126376-4124766 (Counterclockwise)

Preceding gene: 157963193

Following gene: 157963191

Centisome position: 79.74

GC content: 49.22

Gene sequence:

>1611_bases
ATGTCGACATTGAATAACAATAAAGTCCATCCACTAACTAAAGTCTTAATGGTGCAGGGCACCACATCCGATGCGGGCAA
GAGCACCTTGGTCGCGGGCCTATGCCGATTGTTTGTGAGAAAAGGCGTTAAAGTTGCCCCTTTCAAGCCGCAAAATATGG
CGCTTAATAGTGCGGTAACCATAGATGGCGGCGAGATAGGCCGCGCCCAAGCTTTGCAGGCAGTGGCTTGTCATCTCGAG
CCGCAAATCGACTTTAACCCTATTTTATTAAAGCCGAGCTCAGATACCGGATCACAAGTGATAGTACATGGTAAGGCCTT
GATGAAGATGGAAGCCGAAGACTATTTTGGCAAGAATGCCGAAGGTTATCGGGTCAAGGCGATGGCGGCAGTAAAAGAGT
CTTATCATAGATTAACCTCCAATTACGACATGCTGCTGGTGGAAGGGGCGGGCAGTCCTGCCGAGATCAACCTGCGCGAA
GGTGATATCGCCAACATGGGTTTCGCCGAAGAGGTCGACTGTCCGGTGATTATCATTGCCGATATCGATAAAGGAGGTGT
GTTTGCCCATCTAGTTGGCACCTTAGCGCTGCTTAGTGAATCTGAACAGGCGCGAGTCAAAGGCTTTGTGATTAACCGTT
TTCGCGGTGACGTAAGCCTGCTGCAAGATGGCTTAGATTGGCTAGAAAACTACACTAACAAACCTGTACTTGGCGTATTA
CCTTATCTGCATGACCTGCATCTAGACGCCGAAGATGCCTTGATTGCAGCACCTGAAAAATCTTCTGATACTAAGCTGAA
AGTTTTGGTGTTGGTGCTGCCACGTATAAGCAATCACACCGACTTTGATCCTTTAAGGCTTAATCCGCAGATCGACTTCA
ATTATGTGTCGTTGCAGACCCTTGCTAAAAGTCAGGCCCAAGACCAGCAAGCGCTACCGCCAGCGGATTTAATCATCATT
CCAGGCAGTAAAAATGTCCGTGCCGACCTTGAGTTTATCCGTGAACAGGGCTGGGATACGGCAATAGACAAACACCTGCG
TTATGGCGGTAAGGTGTTAGGGATCTGTGGTGGCTACCAGATGCTAGGGCAGGCGATCGACGATCCTAGTGGGATCGAAG
ATATTGCAGGCTGTAGCGAAGGCTTAGGTTTACTGCCGCTGGTAACAGAGTTAACCGCCAACAAAATACTACAGCAAGTC
GAAGGTCATTTAACTCTGCTTGGTGAAACTGCACCTGTTCAGGGTTATGAGATCCACTGCGGTCGCTCGCGCTCATTAAT
GCCACTAGCGAAGCCATTACACCTTAGGGAAAAAAGTGGCGAGCAAGTTAGCAATGAGCAACAACCGGGACAAGCCGAGG
GGATGCTTTCTAGCGATAGTCAGATCATGGGGACCTATCTGCACGGCTTGTTTGATTCGCCCGAGGCGTGCCAACTTATT
TTGAAGTGGGCAGGAATGGAGCAAGCTGAGGCTGTGGATGTCAATCGAGTACGTGAGCAGCAGCTAGACCGACTCGCCGA
TGTGTTAGAACAGCATCTAGATCTCGAGCGACTCAATCAGATCTGGCAAGCTAGCAGGGTAAAAGAACTGAGTGAAAAAC
AACACGTTTGA

Upstream 100 bases:

>100_bases
AGGCAGGCTGGTTGCATCAAGATATAGCGGCCATCGCAGACAGAGTCACTTTAGTGACAGCAGGCTTACCCCTGAGTTTA
AAAGGCTAAAGAAACAGAAC

Downstream 100 bases:

>100_bases
ATGAAAAATAACAGATTCGAACGAAAGCTGAACGTTAGTTGGGCGCTAACTAAACAATTAAATTATTATAGGCGGTCATC
ATAATTGATTTGCTGAAATC

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MSTLNNNKVHPLTKVLMVQGTTSDAGKSTLVAGLCRLFVRKGVKVAPFKPQNMALNSAVTIDGGEIGRAQALQAVACHLE
PQIDFNPILLKPSSDTGSQVIVHGKALMKMEAEDYFGKNAEGYRVKAMAAVKESYHRLTSNYDMLLVEGAGSPAEINLRE
GDIANMGFAEEVDCPVIIIADIDKGGVFAHLVGTLALLSESEQARVKGFVINRFRGDVSLLQDGLDWLENYTNKPVLGVL
PYLHDLHLDAEDALIAAPEKSSDTKLKVLVLVLPRISNHTDFDPLRLNPQIDFNYVSLQTLAKSQAQDQQALPPADLIII
PGSKNVRADLEFIREQGWDTAIDKHLRYGGKVLGICGGYQMLGQAIDDPSGIEDIAGCSEGLGLLPLVTELTANKILQQV
EGHLTLLGETAPVQGYEIHCGRSRSLMPLAKPLHLREKSGEQVSNEQQPGQAEGMLSSDSQIMGTYLHGLFDSPEACQLI
LKWAGMEQAEAVDVNRVREQQLDRLADVLEQHLDLERLNQIWQASRVKELSEKQHV

Sequences:

>Translated_536_residues
MSTLNNNKVHPLTKVLMVQGTTSDAGKSTLVAGLCRLFVRKGVKVAPFKPQNMALNSAVTIDGGEIGRAQALQAVACHLE
PQIDFNPILLKPSSDTGSQVIVHGKALMKMEAEDYFGKNAEGYRVKAMAAVKESYHRLTSNYDMLLVEGAGSPAEINLRE
GDIANMGFAEEVDCPVIIIADIDKGGVFAHLVGTLALLSESEQARVKGFVINRFRGDVSLLQDGLDWLENYTNKPVLGVL
PYLHDLHLDAEDALIAAPEKSSDTKLKVLVLVLPRISNHTDFDPLRLNPQIDFNYVSLQTLAKSQAQDQQALPPADLIII
PGSKNVRADLEFIREQGWDTAIDKHLRYGGKVLGICGGYQMLGQAIDDPSGIEDIAGCSEGLGLLPLVTELTANKILQQV
EGHLTLLGETAPVQGYEIHCGRSRSLMPLAKPLHLREKSGEQVSNEQQPGQAEGMLSSDSQIMGTYLHGLFDSPEACQLI
LKWAGMEQAEAVDVNRVREQQLDRLADVLEQHLDLERLNQIWQASRVKELSEKQHV
>Mature_535_residues
STLNNNKVHPLTKVLMVQGTTSDAGKSTLVAGLCRLFVRKGVKVAPFKPQNMALNSAVTIDGGEIGRAQALQAVACHLEP
QIDFNPILLKPSSDTGSQVIVHGKALMKMEAEDYFGKNAEGYRVKAMAAVKESYHRLTSNYDMLLVEGAGSPAEINLREG
DIANMGFAEEVDCPVIIIADIDKGGVFAHLVGTLALLSESEQARVKGFVINRFRGDVSLLQDGLDWLENYTNKPVLGVLP
YLHDLHLDAEDALIAAPEKSSDTKLKVLVLVLPRISNHTDFDPLRLNPQIDFNYVSLQTLAKSQAQDQQALPPADLIIIP
GSKNVRADLEFIREQGWDTAIDKHLRYGGKVLGICGGYQMLGQAIDDPSGIEDIAGCSEGLGLLPLVTELTANKILQQVE
GHLTLLGETAPVQGYEIHCGRSRSLMPLAKPLHLREKSGEQVSNEQQPGQAEGMLSSDSQIMGTYLHGLFDSPEACQLIL
KWAGMEQAEAVDVNRVREQQLDRLADVLEQHLDLERLNQIWQASRVKELSEKQHV

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.10

Molecular weight: Translated: 58636; Mature: 58505

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLNNNKVHPLTKVLMVQGTTSDAGKSTLVAGLCRLFVRKGVKVAPFKPQNMALNSAVT
CCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCEECCEEE
IDGGEIGRAQALQAVACHLEPQIDFNPILLKPSSDTGSQVIVHGKALMKMEAEDYFGKNA
ECCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEEECHHHHEECHHHHCCCCC
EGYRVKAMAAVKESYHRLTSNYDMLLVEGAGSPAEINLREGDIANMGFAEEVDCPVIIIA
CCEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCEEEEE
DIDKGGVFAHLVGTLALLSESEQARVKGFVINRFRGDVSLLQDGLDWLENYTNKPVLGVL
ECCCCCHHHHHHHHHHHHCCCCHHHHHHEEEEHHCCHHHHHHHHHHHHHHCCCCCHHHHH
PYLHDLHLDAEDALIAAPEKSSDTKLKVLVLVLPRISNHTDFDPLRLNPQIDFNYVSLQT
HHHHHHCCCCCCCEEECCCCCCCCEEEEEEEEECCCCCCCCCCCEEECCCCCCEEEHHHH
LAKSQAQDQQALPPADLIIIPGSKNVRADLEFIREQGWDTAIDKHLRYGGKVLGICGGYQ
HHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCHH
MLGQAIDDPSGIEDIAGCSEGLGLLPLVTELTANKILQQVEGHLTLLGETAPVQGYEIHC
HHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEC
GRSRSLMPLAKPLHLREKSGEQVSNEQQPGQAEGMLSSDSQIMGTYLHGLFDSPEACQLI
CCCCCCCCCCCCHHHHHCCCCHHCCCCCCCHHCCCCCCCHHHHHHHHHHHCCCHHHHHHH
LKWAGMEQAEAVDVNRVREQQLDRLADVLEQHLDLERLNQIWQASRVKELSEKQHV
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STLNNNKVHPLTKVLMVQGTTSDAGKSTLVAGLCRLFVRKGVKVAPFKPQNMALNSAVT
CCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCEECCEEE
IDGGEIGRAQALQAVACHLEPQIDFNPILLKPSSDTGSQVIVHGKALMKMEAEDYFGKNA
ECCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEEECHHHHEECHHHHCCCCC
EGYRVKAMAAVKESYHRLTSNYDMLLVEGAGSPAEINLREGDIANMGFAEEVDCPVIIIA
CCEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCEEEEE
DIDKGGVFAHLVGTLALLSESEQARVKGFVINRFRGDVSLLQDGLDWLENYTNKPVLGVL
ECCCCCHHHHHHHHHHHHCCCCHHHHHHEEEEHHCCHHHHHHHHHHHHHHCCCCCHHHHH
PYLHDLHLDAEDALIAAPEKSSDTKLKVLVLVLPRISNHTDFDPLRLNPQIDFNYVSLQT
HHHHHHCCCCCCCEEECCCCCCCCEEEEEEEEECCCCCCCCCCCEEECCCCCCEEEHHHH
LAKSQAQDQQALPPADLIIIPGSKNVRADLEFIREQGWDTAIDKHLRYGGKVLGICGGYQ
HHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCHH
MLGQAIDDPSGIEDIAGCSEGLGLLPLVTELTANKILQQVEGHLTLLGETAPVQGYEIHC
HHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEC
GRSRSLMPLAKPLHLREKSGEQVSNEQQPGQAEGMLSSDSQIMGTYLHGLFDSPEACQLI
CCCCCCCCCCCCHHHHHCCCCHHCCCCCCCHHCCCCCCCHHHHHHHHHHHCCCHHHHHHH
LKWAGMEQAEAVDVNRVREQQLDRLADVLEQHLDLERLNQIWQASRVKELSEKQHV
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]