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The map label for this gene is carA [H]

Identifier: 157962893

GI number: 157962893

Start: 3772911

End: 3774056

Strand: Reverse

Name: carA [H]

Synonym: Spea_3075

Alternate gene names: 157962893

Gene position: 3774056-3772911 (Counterclockwise)

Preceding gene: 157962894

Following gene: 157962892

Centisome position: 72.93

GC content: 44.15

Gene sequence:

>1146_bases
TTGACAAAGTCTGCCTTACTCGTACTTGAAGATGGAACCATTTTTTCTGGCACTGCAATTGGTGCTGAAGGGATGTCTGT
TGGTGAAGTCGTTTTTAACACTTCAATGACCGGTTACCAAGAAATTTTGACTGATCCATCATATTCACGTCAAATTGTAA
CCCTCACTTATCCACATATTGGTAATACTGGTACCAATGATGAAGACACAGAATCTGATTCTGTTCATGCATGTGGCCTT
ATCATTCGAGACTTGCCGTTAATCGCAAGTAATTTTCGTAATCAGCAATCTTTAAGCGATTATTTGGTCGCTAACAACGT
TGTTGGTATTGCTGATATCGATACTCGTAAATTAACTCGTATCCTACGCGAGAAAGGCGCCCAAGCCGGTTGTATCATGG
TTGGTGATTTAGATGAAGCTAAAGCTTTAGCTGCAGCTAAAGCCTTCCCTGGTCTTAAAGGCATGGATTTAGCCAAAGAA
GTCACTACAAACGAAACGTACTCATGGCGCAGCGGTAGCTGGCGTTTAGTTGGCGGTTTGCCGTCTGACACGCCAGAAGC
TGAGCTTAAGTATAAAGTGGTTGCCTACGACTATGGTGTTAAGCGCAACATCTTACGTATGTTGGTTGACCGCGGTTGTG
ATGTGACGGTTGTGCCTGCACAAACTCCTGCAGCCGAAGTGCTTGCAATGAATCCTGATGGGGTATTCCTGTCGAACGGT
CCTGGCGACCCAGAGCCATGTGATTACGCTATTGCTGCAATCCAAGAAATCTTAAAAACAGACATTCCTGTTTTCGGTAT
CTGCTTGGGTCACCAGTTATTAGCGTTAGCTTCAGGTGCTAAAACACTGAAAATGAAGTTTGGTCATCACGGTGCTAACC
ACCCTGTAAGCAATATTGAGCAAGGTAATGTGATGATCACCAGCCAAAACCATGGTTTTGCTGCTGATGAATCAACGCTG
CCAGCCAACATTAAAGTGACTCACAAGTCTCTGTTTGATGGCTCTTTGCAAGGTATTCATCTTACAGATAAGCCAGCATT
TAGTTTCCAAGGACACCCTGAAGCAAGTCCAGGACCAAATGATGCGGCGCCACTGTTTGATCATTTCATTGATTTGATTG
ACTTGTACCGCAAAAACGCCAAGTAG

Upstream 100 bases:

>100_bases
CGGAATTTGTCAAAATTTTGCATTTTAGCGAAAGTTGGTATTTTAAAAACATATAAAAAACAATTAATTTCAATGATTTG
GCTCTTTTTCGGAGGTCGCG

Downstream 100 bases:

>100_bases
TAACCTCATCCTGGAGAAGATTTAAGCAATGCCAAAACGTACAGATATAAAGAGTATTCTTATCCTAGGTGCTGGACCGA
TTGTAATTGGTCAAGCCTGT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MTKSALLVLEDGTIFSGTAIGAEGMSVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNTGTNDEDTESDSVHACGL
IIRDLPLIASNFRNQQSLSDYLVANNVVGIADIDTRKLTRILREKGAQAGCIMVGDLDEAKALAAAKAFPGLKGMDLAKE
VTTNETYSWRSGSWRLVGGLPSDTPEAELKYKVVAYDYGVKRNILRMLVDRGCDVTVVPAQTPAAEVLAMNPDGVFLSNG
PGDPEPCDYAIAAIQEILKTDIPVFGICLGHQLLALASGAKTLKMKFGHHGANHPVSNIEQGNVMITSQNHGFAADESTL
PANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPNDAAPLFDHFIDLIDLYRKNAK

Sequences:

>Translated_381_residues
MTKSALLVLEDGTIFSGTAIGAEGMSVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNTGTNDEDTESDSVHACGL
IIRDLPLIASNFRNQQSLSDYLVANNVVGIADIDTRKLTRILREKGAQAGCIMVGDLDEAKALAAAKAFPGLKGMDLAKE
VTTNETYSWRSGSWRLVGGLPSDTPEAELKYKVVAYDYGVKRNILRMLVDRGCDVTVVPAQTPAAEVLAMNPDGVFLSNG
PGDPEPCDYAIAAIQEILKTDIPVFGICLGHQLLALASGAKTLKMKFGHHGANHPVSNIEQGNVMITSQNHGFAADESTL
PANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPNDAAPLFDHFIDLIDLYRKNAK
>Mature_380_residues
TKSALLVLEDGTIFSGTAIGAEGMSVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNTGTNDEDTESDSVHACGLI
IRDLPLIASNFRNQQSLSDYLVANNVVGIADIDTRKLTRILREKGAQAGCIMVGDLDEAKALAAAKAFPGLKGMDLAKEV
TTNETYSWRSGSWRLVGGLPSDTPEAELKYKVVAYDYGVKRNILRMLVDRGCDVTVVPAQTPAAEVLAMNPDGVFLSNGP
GDPEPCDYAIAAIQEILKTDIPVFGICLGHQLLALASGAKTLKMKFGHHGANHPVSNIEQGNVMITSQNHGFAADESTLP
ANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPNDAAPLFDHFIDLIDLYRKNAK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=380, Percent_Identity=37.6315789473684, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI169790915, Length=383, Percent_Identity=36.5535248041775, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI21361331, Length=383, Percent_Identity=36.5535248041775, Blast_Score=216, Evalue=3e-56,
Organism=Escherichia coli, GI1786215, Length=382, Percent_Identity=78.0104712041885, Blast_Score=623, Evalue=1e-180,
Organism=Caenorhabditis elegans, GI193204318, Length=382, Percent_Identity=37.696335078534, Blast_Score=234, Evalue=6e-62,
Organism=Saccharomyces cerevisiae, GI6324878, Length=388, Percent_Identity=39.6907216494845, Blast_Score=247, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6322331, Length=400, Percent_Identity=36, Blast_Score=231, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6322638, Length=162, Percent_Identity=29.6296296296296, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45555749, Length=387, Percent_Identity=39.2764857881137, Blast_Score=230, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24642586, Length=386, Percent_Identity=39.3782383419689, Blast_Score=230, Evalue=2e-60,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40871; Mature: 40740

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSALLVLEDGTIFSGTAIGAEGMSVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHI
CCCCEEEEEECCCEEECCEECCCCCCHHHEEECCCCCHHHHHHCCCCCCCEEEEEECCCC
GNTGTNDEDTESDSVHACGLIIRDLPLIASNFRNQQSLSDYLVANNVVGIADIDTRKLTR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEECCHHHHHH
ILREKGAQAGCIMVGDLDEAKALAAAKAFPGLKGMDLAKEVTTNETYSWRSGSWRLVGGL
HHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEECCCCEEEECCC
PSDTPEAELKYKVVAYDYGVKRNILRMLVDRGCDVTVVPAQTPAAEVLAMNPDGVFLSNG
CCCCCCCCEEEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCEEECCC
PGDPEPCDYAIAAIQEILKTDIPVFGICLGHQLLALASGAKTLKMKFGHHGANHPVSNIE
CCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
QGNVMITSQNHGFAADESTLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPN
CCCEEEEECCCCCCCCCCCCCCEEEEEEHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCC
DAAPLFDHFIDLIDLYRKNAK
CCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKSALLVLEDGTIFSGTAIGAEGMSVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHI
CCCEEEEEECCCEEECCEECCCCCCHHHEEECCCCCHHHHHHCCCCCCCEEEEEECCCC
GNTGTNDEDTESDSVHACGLIIRDLPLIASNFRNQQSLSDYLVANNVVGIADIDTRKLTR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEECCHHHHHH
ILREKGAQAGCIMVGDLDEAKALAAAKAFPGLKGMDLAKEVTTNETYSWRSGSWRLVGGL
HHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEECCCCEEEECCC
PSDTPEAELKYKVVAYDYGVKRNILRMLVDRGCDVTVVPAQTPAAEVLAMNPDGVFLSNG
CCCCCCCCEEEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCEEECCC
PGDPEPCDYAIAAIQEILKTDIPVFGICLGHQLLALASGAKTLKMKFGHHGANHPVSNIE
CCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
QGNVMITSQNHGFAADESTLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPN
CCCEEEEECCCCCCCCCCCCCCEEEEEEHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCC
DAAPLFDHFIDLIDLYRKNAK
CCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]