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The map label for this gene is prfC [H]

Identifier: 157962871

GI number: 157962871

Start: 3743934

End: 3745514

Strand: Reverse

Name: prfC [H]

Synonym: Spea_3053

Alternate gene names: 157962871

Gene position: 3745514-3743934 (Counterclockwise)

Preceding gene: 157962872

Following gene: 157962870

Centisome position: 72.38

GC content: 45.48

Gene sequence:

>1581_bases
ATGTCAGGCTATAAAGTTGAGGTCGACAAACGTCGCACTTTTGCCATCATCTCTCACCCAGATGCGGGTAAAACCACCAT
TACTGAAAAGGTTCTTTTATTCGGAAACGCGTTACAAAAGGCGGGTACGGTAAAAGGCAAAAAGTCAGGTCAGCACGCTA
AGTCTGACTGGATGGAGATGGAAAAAGATCGTGGGATCTCAATTACGACCTCTGTAATGCAGTTTCCATACAGCGACGCG
CTGGTAAACTTGCTAGATACGCCTGGTCACGAAGACTTCTCTGAAGATACTTACCGTACATTAACGGCGGTAGACTCATG
CTTAATGGTTATCGACTCAGCGAAAGGTGTTGAGCAACGTACTATTAAACTAATGGAAGTCACTCGTTTACGTGATACTC
CAATTGTTACCTTCATGAACAAGCTTGACCGTGATATTCGCGATCCAATCGAGTTGATGGATGAAGTTGAAGAAGTGCTC
AACATTAAGTGCGCACCTATCACTTGGCCAATCGGTGCGGGTAAAGAATTCAAAGGTGTTTATCACCTATTACGTGATGA
AGTGATCCTGTATCAAGGTGGTATGGGGCATACAATCCAAGACTCTCGGGTCATCAAGGGGCTAGATAACCCAGAGCTTG
ATGAGGCGATTGGCAGCTATGCTGCTGAAATCCGTGATGAAATGGAATTGGTTGTTGGCGCGTCACATGAATTCGATCAC
CAGCAATTCTTAAAAGGCGAATTAACTCCTGTTTATTTTGGAACTGCGCTAGGTAACTTTGGCGTCGACCATATTCTTGA
CGGTATCGTTGAGTGGGCACCAGTCCCACAGCCTCGCGAAACTGAAATTCGTGAAGTTCAACCTGAAGAAGAAAAGTTCA
GTGGTTTCGTATTTAAGATCCAAGCGAATATGGATCCTAAGCATCGAGATCGTGTTGCCTTTATGCGAATTTGTTCAGGC
CGTTACGAGCAAGGCATGAAGATGCATCATGTACGTCTTGGTAAAGATGTTAACGTCAGTGACGCGTTGACCTTTATGGC
CGGCGATCGTAACCGCGCCGAAGCTGCGTACCCAGGCGATATTATCGGCTTACATAACCATGGCACAATCCGTATTGGTG
ATACCTTTACCCAAGGCGAGAAGCTTCGCTTTACTGGTGTACCAAACTTTGCGCCAGAGATGTTCCGTCGTATTCGTTTA
AAAGATCCGTTGAAGCAGAAGCAGTTGCTTAAAGGTCTGGTACAGCTTTCAGAAGAAGGCGCAGTACAGGTGTTTAGACC
ACTCGATAGTAATGACTTAATTGTGGGTGCCGTTGGTGTGCTGCAGTTTGAGGTTGTTGTAGGTCGTCTAAAGACTGAAT
ATAAAGTTGAAGCGATTTACGAAGCGATCAGTGTTGCGACTGCGCGTTGGGTGTACTGTGACGATCATAAGAAGCTCGAT
GAGTTCAAGCGTAAGTGCAGCATGAACTTAGCGTTAGATGGTGGTGACAACCTGACTTATATCGCACCGACTATGGTTAA
TCTTAACCTGTCGATGGAACGTTACCCAGATATTGAATTTGCTAAGACGCGCGAAAACTAA

Upstream 100 bases:

>100_bases
ATGGTGGTGATGCTCATTTTAGCCGCTTAGTGTGGTAGCTAAAAGATAGGTGAGCGGCTATAATTGCGGCCTTTGTGAAC
TCCCAGAATTACAGGTATAC

Downstream 100 bases:

>100_bases
TACGCGGTTAATTATTCGATACTAAAACCCCAGGCTATGCCTGGGGTTTTGTTTTTTAAAGGACGGAATTAGCTACCCTT
GCTATTTTATTGGTATGGAC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSGYKVEVDKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEMEKDRGISITTSVMQFPYSDA
LVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEQRTIKLMEVTRLRDTPIVTFMNKLDRDIRDPIELMDEVEEVL
NIKCAPITWPIGAGKEFKGVYHLLRDEVILYQGGMGHTIQDSRVIKGLDNPELDEAIGSYAAEIRDEMELVVGASHEFDH
QQFLKGELTPVYFGTALGNFGVDHILDGIVEWAPVPQPRETEIREVQPEEEKFSGFVFKIQANMDPKHRDRVAFMRICSG
RYEQGMKMHHVRLGKDVNVSDALTFMAGDRNRAEAAYPGDIIGLHNHGTIRIGDTFTQGEKLRFTGVPNFAPEMFRRIRL
KDPLKQKQLLKGLVQLSEEGAVQVFRPLDSNDLIVGAVGVLQFEVVVGRLKTEYKVEAIYEAISVATARWVYCDDHKKLD
EFKRKCSMNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREN

Sequences:

>Translated_526_residues
MSGYKVEVDKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEMEKDRGISITTSVMQFPYSDA
LVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEQRTIKLMEVTRLRDTPIVTFMNKLDRDIRDPIELMDEVEEVL
NIKCAPITWPIGAGKEFKGVYHLLRDEVILYQGGMGHTIQDSRVIKGLDNPELDEAIGSYAAEIRDEMELVVGASHEFDH
QQFLKGELTPVYFGTALGNFGVDHILDGIVEWAPVPQPRETEIREVQPEEEKFSGFVFKIQANMDPKHRDRVAFMRICSG
RYEQGMKMHHVRLGKDVNVSDALTFMAGDRNRAEAAYPGDIIGLHNHGTIRIGDTFTQGEKLRFTGVPNFAPEMFRRIRL
KDPLKQKQLLKGLVQLSEEGAVQVFRPLDSNDLIVGAVGVLQFEVVVGRLKTEYKVEAIYEAISVATARWVYCDDHKKLD
EFKRKCSMNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREN
>Mature_525_residues
SGYKVEVDKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEMEKDRGISITTSVMQFPYSDAL
VNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEQRTIKLMEVTRLRDTPIVTFMNKLDRDIRDPIELMDEVEEVLN
IKCAPITWPIGAGKEFKGVYHLLRDEVILYQGGMGHTIQDSRVIKGLDNPELDEAIGSYAAEIRDEMELVVGASHEFDHQ
QFLKGELTPVYFGTALGNFGVDHILDGIVEWAPVPQPRETEIREVQPEEEKFSGFVFKIQANMDPKHRDRVAFMRICSGR
YEQGMKMHHVRLGKDVNVSDALTFMAGDRNRAEAAYPGDIIGLHNHGTIRIGDTFTQGEKLRFTGVPNFAPEMFRRIRLK
DPLKQKQLLKGLVQLSEEGAVQVFRPLDSNDLIVGAVGVLQFEVVVGRLKTEYKVEAIYEAISVATARWVYCDDHKKLDE
FKRKCSMNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREN

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=498, Percent_Identity=26.3052208835341, Blast_Score=165, Evalue=9e-41,
Organism=Homo sapiens, GI19923640, Length=510, Percent_Identity=26.8627450980392, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI25306283, Length=394, Percent_Identity=27.9187817258883, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI25306287, Length=482, Percent_Identity=25.7261410788382, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI157426893, Length=183, Percent_Identity=28.9617486338798, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI94966754, Length=139, Percent_Identity=30.9352517985612, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=28.1879194630872, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=32.7272727272727, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=32.7272727272727, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=32.7272727272727, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1790835, Length=527, Percent_Identity=76.8500948766603, Blast_Score=859, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=498, Percent_Identity=25.7028112449799, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=31.4102564102564, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI48994988, Length=198, Percent_Identity=29.7979797979798, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=484, Percent_Identity=27.2727272727273, Blast_Score=165, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17556745, Length=485, Percent_Identity=22.8865979381443, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17557151, Length=135, Percent_Identity=31.1111111111111, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17506493, Length=178, Percent_Identity=28.6516853932584, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=480, Percent_Identity=26.6666666666667, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6322359, Length=428, Percent_Identity=26.1682242990654, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324707, Length=156, Percent_Identity=30.7692307692308, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320593, Length=156, Percent_Identity=30.7692307692308, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=158, Percent_Identity=23.4177215189873, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24582462, Length=479, Percent_Identity=26.3048016701461, Blast_Score=169, Evalue=6e-42,
Organism=Drosophila melanogaster, GI221458488, Length=392, Percent_Identity=26.2755102040816, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=29.559748427673, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24585709, Length=169, Percent_Identity=30.1775147928994, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24585711, Length=169, Percent_Identity=30.1775147928994, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24585713, Length=169, Percent_Identity=30.1775147928994, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59353; Mature: 59222

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGYKVEVDKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEM
CCCCEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
EKDRGISITTSVMQFPYSDALVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEQR
HHCCCCEEEHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
TIKLMEVTRLRDTPIVTFMNKLDRDIRDPIELMDEVEEVLNIKCAPITWPIGAGKEFKGV
HHHHHHHHHHCCCHHHHHHHHHCHHHCCHHHHHHHHHHHHCCEECEEEECCCCCCHHHHH
YHLLRDEVILYQGGMGHTIQDSRVIKGLDNPELDEAIGSYAAEIRDEMELVVGASHEFDH
HHHHHCCEEEEECCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCH
QQFLKGELTPVYFGTALGNFGVDHILDGIVEWAPVPQPRETEIREVQPEEEKFSGFVFKI
HHHHCCCCCEEEEEHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHCCCEEEEE
QANMDPKHRDRVAFMRICSGRYEQGMKMHHVRLGKDVNVSDALTFMAGDRNRAEAAYPGD
ECCCCCCHHHHHHHHHHHCCHHHCCCEEEEEECCCCCCHHHHEEEEECCCCCCCCCCCCC
IIGLHNHGTIRIGDTFTQGEKLRFTGVPNFAPEMFRRIRLKDPLKQKQLLKGLVQLSEEG
EEEECCCCEEEECCCCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
AVQVFRPLDSNDLIVGAVGVLQFEVVVGRLKTEYKVEAIYEAISVATARWVYCDDHKKLD
CEEEEECCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEEECCHHHHH
EFKRKCSMNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREN
HHHHHHCCEEEEECCCCEEEEEEEEEEEEECHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SGYKVEVDKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEM
CCCEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
EKDRGISITTSVMQFPYSDALVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEQR
HHCCCCEEEHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
TIKLMEVTRLRDTPIVTFMNKLDRDIRDPIELMDEVEEVLNIKCAPITWPIGAGKEFKGV
HHHHHHHHHHCCCHHHHHHHHHCHHHCCHHHHHHHHHHHHCCEECEEEECCCCCCHHHHH
YHLLRDEVILYQGGMGHTIQDSRVIKGLDNPELDEAIGSYAAEIRDEMELVVGASHEFDH
HHHHHCCEEEEECCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCH
QQFLKGELTPVYFGTALGNFGVDHILDGIVEWAPVPQPRETEIREVQPEEEKFSGFVFKI
HHHHCCCCCEEEEEHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHCCCEEEEE
QANMDPKHRDRVAFMRICSGRYEQGMKMHHVRLGKDVNVSDALTFMAGDRNRAEAAYPGD
ECCCCCCHHHHHHHHHHHCCHHHCCCEEEEEECCCCCCHHHHEEEEECCCCCCCCCCCCC
IIGLHNHGTIRIGDTFTQGEKLRFTGVPNFAPEMFRRIRLKDPLKQKQLLKGLVQLSEEG
EEEECCCCEEEECCCCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
AVQVFRPLDSNDLIVGAVGVLQFEVVVGRLKTEYKVEAIYEAISVATARWVYCDDHKKLD
CEEEEECCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEEECCHHHHH
EFKRKCSMNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREN
HHHHHHCCEEEEECCCCEEEEEEEEEEEEECHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA