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The map label for this gene is grpE [H]

Identifier: 157962810

GI number: 157962810

Start: 3664697

End: 3665299

Strand: Reverse

Name: grpE [H]

Synonym: Spea_2992

Alternate gene names: 157962810

Gene position: 3665299-3664697 (Counterclockwise)

Preceding gene: 157962817

Following gene: 157962806

Centisome position: 70.83

GC content: 44.28

Gene sequence:

>603_bases
ATGAGCAACCAAACAAATAAAGCACAAGATAACCAAGTCGAAGAGATCGTAGAAGGCGAATTGCTTAACGAGAACGCTAC
AGAAGCGACTGATGAAGCCTCTTTGATGGATGAGCTTACTCAAGCTAACTTCCGCGTAGAAGAGTTAGAGAAGGCTTTGC
AAGAAGCTGAAGCTAAAGTCGATGCGCAAAAAGATTCAGTGATCCGTGCAGCGGCTGAAGTTGACAACATTCGTCGTCGT
TCGGCAATGGATGTAGAAAAAGCGCACAAGTTCGCGTTAGAAAAGTTCATCAATGAATTGTTACCTGTATTAGATAACAT
GGAGCGAGCTCTGCAAGGTACCGATGCAGAAGCTGAAGCGACCAAAGCTATTTATGAAGGTGTTGAACTGACGGCTAAGA
GCTTTATGAGCACAGTTGAGAAGTTTGGTCTAGTTCAAGTTGATCCACAAGGTGATACCTTCAACCCTGAGTTACATCAA
GCTATTGGCATGCAACCAAGCGCCGATTTTCCTGCAAACACAGTTATGATGGTTATGCAGAAGGGCTACACATTGAATGA
GCGTCTATTGCGCCCAGCAATGGTTATGGTTTCTCAAGGCTAA

Upstream 100 bases:

>100_bases
AAATTATGTTAGATCCAGCTTTAGGCCTTGAAACCTTAAATTTGATCCCCATAATATGCTCAGAGCATGCAGAAACAAAG
CATTTTTAGCTGGAGCAAAA

Downstream 100 bases:

>100_bases
TGATTCGCTAGAGACAAGCTTGTGCTAACCATTGAGTGAGTACTGGCAATAAAAAAAGGACTGCCATGGCAGTCCTTTTT
ATTTAGCTATTCTCTTACAG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MSNQTNKAQDNQVEEIVEGELLNENATEATDEASLMDELTQANFRVEELEKALQEAEAKVDAQKDSVIRAAAEVDNIRRR
SAMDVEKAHKFALEKFINELLPVLDNMERALQGTDAEAEATKAIYEGVELTAKSFMSTVEKFGLVQVDPQGDTFNPELHQ
AIGMQPSADFPANTVMMVMQKGYTLNERLLRPAMVMVSQG

Sequences:

>Translated_200_residues
MSNQTNKAQDNQVEEIVEGELLNENATEATDEASLMDELTQANFRVEELEKALQEAEAKVDAQKDSVIRAAAEVDNIRRR
SAMDVEKAHKFALEKFINELLPVLDNMERALQGTDAEAEATKAIYEGVELTAKSFMSTVEKFGLVQVDPQGDTFNPELHQ
AIGMQPSADFPANTVMMVMQKGYTLNERLLRPAMVMVSQG
>Mature_199_residues
SNQTNKAQDNQVEEIVEGELLNENATEATDEASLMDELTQANFRVEELEKALQEAEAKVDAQKDSVIRAAAEVDNIRRRS
AMDVEKAHKFALEKFINELLPVLDNMERALQGTDAEAEATKAIYEGVELTAKSFMSTVEKFGLVQVDPQGDTFNPELHQA
IGMQPSADFPANTVMMVMQKGYTLNERLLRPAMVMVSQG

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=157, Percent_Identity=33.7579617834395, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI40255109, Length=137, Percent_Identity=32.1167883211679, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1788967, Length=201, Percent_Identity=40.7960199004975, Blast_Score=145, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17552458, Length=141, Percent_Identity=36.8794326241135, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324806, Length=168, Percent_Identity=33.3333333333333, Blast_Score=101, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24653432, Length=178, Percent_Identity=30.3370786516854, Blast_Score=84, Evalue=5e-17,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 22209; Mature: 22078

Theoretical pI: Translated: 4.16; Mature: 4.16

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQTNKAQDNQVEEIVEGELLNENATEATDEASLMDELTQANFRVEELEKALQEAEAKV
CCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DAQKDSVIRAAAEVDNIRRRSAMDVEKAHKFALEKFINELLPVLDNMERALQGTDAEAEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
TKAIYEGVELTAKSFMSTVEKFGLVQVDPQGDTFNPELHQAIGMQPSADFPANTVMMVMQ
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHH
KGYTLNERLLRPAMVMVSQG
CCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNQTNKAQDNQVEEIVEGELLNENATEATDEASLMDELTQANFRVEELEKALQEAEAKV
CCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DAQKDSVIRAAAEVDNIRRRSAMDVEKAHKFALEKFINELLPVLDNMERALQGTDAEAEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
TKAIYEGVELTAKSFMSTVEKFGLVQVDPQGDTFNPELHQAIGMQPSADFPANTVMMVMQ
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHH
KGYTLNERLLRPAMVMVSQG
CCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA