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The map label for this gene is leuO [H]

Identifier: 157962722

GI number: 157962722

Start: 3538979

End: 3539896

Strand: Reverse

Name: leuO [H]

Synonym: Spea_2903

Alternate gene names: 157962722

Gene position: 3539896-3538979 (Counterclockwise)

Preceding gene: 157962724

Following gene: 157962720

Centisome position: 68.41

GC content: 45.97

Gene sequence:

>918_bases
ATGTCACAGCAGAAGTCGTTCGATCTTAATCTATTTCGTATCTTTACTGCGGTGTACCGTACCTCGTCATTTAGTCGCGC
AGCCGAGGAGCTGGATTTGACTCAATCGTCGGTGAGCAACGCTATTACGCGGCTTAAACTCGCGGTGGGGGAGGAGCTGT
TTATTCGCGTAGGCAGGGGAGTTAAGCCAACGGCGAGTGCGATGAACTTGTATCAGCAGCTTGCAAGTCCCGTATCTGAA
ATTGAGCAGGTAGCTTCAAGCTTTGAGCTCTTTGATGCAAAGCGTAGTGAACGCACCTTCTATGTTTATGCCAGTGAGTC
TGCGATTTTGTTGCTGCAGGGGATTGTTGAGCAGTTCCTTGAAGAGTTGTCGATTAATCTGGTGTTTCGTGAACCACCAT
TAATCGAAGAGGAGCTGCAGATGGATCTGCAACTTGAGAAAGTGGATTTAGCACTGGATATCTGGCTACCGGAATCGAGC
TCTTTCAAGAGTCAGAAGGTGATGCAGGATAAATTGGTGTGTGTGGCCAGAAAAGGCCACCCGCGTATAAGTGGTGCGAT
TACGCAGGAGCAGTATTTTAATGAAAAGCATATCATCTTTAAGATGCGCCGAGATAACTTAAGTGTTGCAGATCTTTTAA
CCAAGCAAGTTTTGCCTGCTCGCAAGATGCACAGTGAACAATCATCAATTTTAAGTATGATGGCCTTGGCCTCTAAAAGT
GATGCCTTAGCTATTGTGTCTAAGTCTTATACCGATGAGTATGCACAGCTGTTTGGCTTACAGGTATTTGAGCTGCCCTT
TGCGGCAGAGCCTATCGAGTATTACATGGTATGGAGTCATAAACTGCAGCAAAACCGTGCCAATATTTGGTTAAGGGATT
TGCTTAATCAAGCGATAACACGGCGAACACAGGCTTAG

Upstream 100 bases:

>100_bases
TAACCATGGTCATTTGATTAATAATTAGCGTAGACTTTACTTTTAATATTTATAATATGAATATTAAGTCTGATGTTATT
AACGGTAAGCGAAACAATTT

Downstream 100 bases:

>100_bases
TTTTAGCTTTGAACTTAATAAAAAAGACCTGTAATGCAGGGCGTTACAGGTCTTTGCTTGGAGTTCGATTATTGTCTGGC
GAGACGTTGATTTTCAGCCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MSQQKSFDLNLFRIFTAVYRTSSFSRAAEELDLTQSSVSNAITRLKLAVGEELFIRVGRGVKPTASAMNLYQQLASPVSE
IEQVASSFELFDAKRSERTFYVYASESAILLLQGIVEQFLEELSINLVFREPPLIEEELQMDLQLEKVDLALDIWLPESS
SFKSQKVMQDKLVCVARKGHPRISGAITQEQYFNEKHIIFKMRRDNLSVADLLTKQVLPARKMHSEQSSILSMMALASKS
DALAIVSKSYTDEYAQLFGLQVFELPFAAEPIEYYMVWSHKLQQNRANIWLRDLLNQAITRRTQA

Sequences:

>Translated_305_residues
MSQQKSFDLNLFRIFTAVYRTSSFSRAAEELDLTQSSVSNAITRLKLAVGEELFIRVGRGVKPTASAMNLYQQLASPVSE
IEQVASSFELFDAKRSERTFYVYASESAILLLQGIVEQFLEELSINLVFREPPLIEEELQMDLQLEKVDLALDIWLPESS
SFKSQKVMQDKLVCVARKGHPRISGAITQEQYFNEKHIIFKMRRDNLSVADLLTKQVLPARKMHSEQSSILSMMALASKS
DALAIVSKSYTDEYAQLFGLQVFELPFAAEPIEYYMVWSHKLQQNRANIWLRDLLNQAITRRTQA
>Mature_304_residues
SQQKSFDLNLFRIFTAVYRTSSFSRAAEELDLTQSSVSNAITRLKLAVGEELFIRVGRGVKPTASAMNLYQQLASPVSEI
EQVASSFELFDAKRSERTFYVYASESAILLLQGIVEQFLEELSINLVFREPPLIEEELQMDLQLEKVDLALDIWLPESSS
FKSQKVMQDKLVCVARKGHPRISGAITQEQYFNEKHIIFKMRRDNLSVADLLTKQVLPARKMHSEQSSILSMMALASKSD
ALAIVSKSYTDEYAQLFGLQVFELPFAAEPIEYYMVWSHKLQQNRANIWLRDLLNQAITRRTQA

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=301, Percent_Identity=26.9102990033223, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI87081766, Length=265, Percent_Identity=27.1698113207547, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1786820, Length=283, Percent_Identity=25.0883392226148, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789204, Length=255, Percent_Identity=24.7058823529412, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34861; Mature: 34730

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQKSFDLNLFRIFTAVYRTSSFSRAAEELDLTQSSVSNAITRLKLAVGEELFIRVGRG
CCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCC
VKPTASAMNLYQQLASPVSEIEQVASSFELFDAKRSERTFYVYASESAILLLQGIVEQFL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHH
EELSINLVFREPPLIEEELQMDLQLEKVDLALDIWLPESSSFKSQKVMQDKLVCVARKGH
HHCCEEEEECCCCCCHHHHHHCCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHEEECCCC
PRISGAITQEQYFNEKHIIFKMRRDNLSVADLLTKQVLPARKMHSEQSSILSMMALASKS
CCCCCCCCHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
DALAIVSKSYTDEYAQLFGLQVFELPFAAEPIEYYMVWSHKLQQNRANIWLRDLLNQAIT
CCEEEEEHHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRTQA
HHCCC
>Mature Secondary Structure 
SQQKSFDLNLFRIFTAVYRTSSFSRAAEELDLTQSSVSNAITRLKLAVGEELFIRVGRG
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCC
VKPTASAMNLYQQLASPVSEIEQVASSFELFDAKRSERTFYVYASESAILLLQGIVEQFL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHH
EELSINLVFREPPLIEEELQMDLQLEKVDLALDIWLPESSSFKSQKVMQDKLVCVARKGH
HHCCEEEEECCCCCCHHHHHHCCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHEEECCCC
PRISGAITQEQYFNEKHIIFKMRRDNLSVADLLTKQVLPARKMHSEQSSILSMMALASKS
CCCCCCCCHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
DALAIVSKSYTDEYAQLFGLQVFELPFAAEPIEYYMVWSHKLQQNRANIWLRDLLNQAIT
CCEEEEEHHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRTQA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 3519576 [H]