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The map label for this gene is mltB [H]

Identifier: 157962263

GI number: 157962263

Start: 2986307

End: 2987302

Strand: Reverse

Name: mltB [H]

Synonym: Spea_2442

Alternate gene names: 157962263

Gene position: 2987302-2986307 (Counterclockwise)

Preceding gene: 157962264

Following gene: 157962262

Centisome position: 57.73

GC content: 43.88

Gene sequence:

>996_bases
ATGTTTTTAGGGAAAAAGATTCTGACTTTAGCGTGTTCTTTGAGTTTTTTATCGTCCACAGCAATGGCCGAGAGTGAGAC
CCAAGGAACGTTTAGTAACTATGTTGCTGAGCTTAAGCAAGATGCGATAGATAAAGGTTTCAGTCAGCAAACGATTGATG
AGGTTTTCCCTAAAATAAAGATGTTTAAAAAGGCGCTGGTTTCCAATAAAGACCCTATTGAAGATCCCATTACCCTTGAG
ACATACCTCCCACAAATAGTACCTCAAACAAAGGTTGATCTGGCTAGAGCGTTTTACAAGCAACACTACACCGAGCTTGA
AAAAATTGGCGAACGTTATGGCGTTCAGCCGCGCTTTATTGTGGCGCTTTTGGGCATAGAGTCTAACCTTGGCCAAGATG
CGGGTGGCTACCCTGTATTATCAGTTACAGCCTCTCTAGCCTATCAAGGTCAGCGCGAAGCCCATTTCCGTCAAGAGTTT
TTTGCAGCTCTAAAGATTGTCGACCAAGGCCATATCGATTTTTCCGAGTTAAAGGGCACTTCGACAGGAACCATGGGGCA
GCCTCTGTTTTTACCTAGTATCTATCTAGCTTACGCTCAAGATGGCGATGGCGATGGTAGAAAGGATATCTGGAGTAATA
ACAGTGATGTTTTTGCATCAGCAGCTTATTATCTGCAGCAGGCTGGCTGGAAAGCAACGGAAACCTGGGGCAGGCAAGTT
AAGGTCCCCAATCAATTTCAGTCTTCTCTTGCTGACTTGAGTATCAAGAAAAGCTTTAATGAGTGGCAAGCTTTGGGCGT
TAGGCGTTTTGACGGCAGTGACTTACCCAAACGAGAAGATATGCAGATATCTATGGTGATGCCTGACGGCATTGCGGGGC
GTAGCTATCTTATTTATGATAATTACCGCGGTTTGCTGCAATGGAATAATTCAGACTACTTTGCTATTTCCGTTACCTAC
CTTTCAGAAAGAATTAAGTATCCTCCGATCATCTAA

Upstream 100 bases:

>100_bases
CTCCAGTTGTTAATTTACTAGAACAACATAAGAAAGAGATCGGTTTTAATCCAGATTAAATCAGCGATTAAAGCCACAAA
AGATCTAATAAAGGGGATTT

Downstream 100 bases:

>100_bases
TTGCGGGTGTTATAGTCAGTTTAATGAATAGCGGCCTAGAGTCGCTATTTTTGTTTAAATAAGTCGCAGTCGATGTAATT
TTGAGCATCAGAGTCACAAA

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MFLGKKILTLACSLSFLSSTAMAESETQGTFSNYVAELKQDAIDKGFSQQTIDEVFPKIKMFKKALVSNKDPIEDPITLE
TYLPQIVPQTKVDLARAFYKQHYTELEKIGERYGVQPRFIVALLGIESNLGQDAGGYPVLSVTASLAYQGQREAHFRQEF
FAALKIVDQGHIDFSELKGTSTGTMGQPLFLPSIYLAYAQDGDGDGRKDIWSNNSDVFASAAYYLQQAGWKATETWGRQV
KVPNQFQSSLADLSIKKSFNEWQALGVRRFDGSDLPKREDMQISMVMPDGIAGRSYLIYDNYRGLLQWNNSDYFAISVTY
LSERIKYPPII

Sequences:

>Translated_331_residues
MFLGKKILTLACSLSFLSSTAMAESETQGTFSNYVAELKQDAIDKGFSQQTIDEVFPKIKMFKKALVSNKDPIEDPITLE
TYLPQIVPQTKVDLARAFYKQHYTELEKIGERYGVQPRFIVALLGIESNLGQDAGGYPVLSVTASLAYQGQREAHFRQEF
FAALKIVDQGHIDFSELKGTSTGTMGQPLFLPSIYLAYAQDGDGDGRKDIWSNNSDVFASAAYYLQQAGWKATETWGRQV
KVPNQFQSSLADLSIKKSFNEWQALGVRRFDGSDLPKREDMQISMVMPDGIAGRSYLIYDNYRGLLQWNNSDYFAISVTY
LSERIKYPPII
>Mature_331_residues
MFLGKKILTLACSLSFLSSTAMAESETQGTFSNYVAELKQDAIDKGFSQQTIDEVFPKIKMFKKALVSNKDPIEDPITLE
TYLPQIVPQTKVDLARAFYKQHYTELEKIGERYGVQPRFIVALLGIESNLGQDAGGYPVLSVTASLAYQGQREAHFRQEF
FAALKIVDQGHIDFSELKGTSTGTMGQPLFLPSIYLAYAQDGDGDGRKDIWSNNSDVFASAAYYLQQAGWKATETWGRQV
KVPNQFQSSLADLSIKKSFNEWQALGVRRFDGSDLPKREDMQISMVMPDGIAGRSYLIYDNYRGLLQWNNSDYFAISVTY
LSERIKYPPII

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=307, Percent_Identity=26.3843648208469, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 37244; Mature: 37244

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLGKKILTLACSLSFLSSTAMAESETQGTFSNYVAELKQDAIDKGFSQQTIDEVFPKIK
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
MFKKALVSNKDPIEDPITLETYLPQIVPQTKVDLARAFYKQHYTELEKIGERYGVQPRFI
HHHHHHHCCCCCCCCCEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHE
VALLGIESNLGQDAGGYPVLSVTASLAYQGQREAHFRQEFFAALKIVDQGHIDFSELKGT
EEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCC
STGTMGQPLFLPSIYLAYAQDGDGDGRKDIWSNNSDVFASAAYYLQQAGWKATETWGRQV
CCCCCCCCHHHHHEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCEE
KVPNQFQSSLADLSIKKSFNEWQALGVRRFDGSDLPKREDMQISMVMPDGIAGRSYLIYD
CCCHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCEEEEECCCCCCCCEEEEEE
NYRGLLQWNNSDYFAISVTYLSERIKYPPII
CCCEEEEECCCCEEEEEEHHHHHHCCCCCCC
>Mature Secondary Structure
MFLGKKILTLACSLSFLSSTAMAESETQGTFSNYVAELKQDAIDKGFSQQTIDEVFPKIK
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
MFKKALVSNKDPIEDPITLETYLPQIVPQTKVDLARAFYKQHYTELEKIGERYGVQPRFI
HHHHHHHCCCCCCCCCEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHE
VALLGIESNLGQDAGGYPVLSVTASLAYQGQREAHFRQEFFAALKIVDQGHIDFSELKGT
EEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCC
STGTMGQPLFLPSIYLAYAQDGDGDGRKDIWSNNSDVFASAAYYLQQAGWKATETWGRQV
CCCCCCCCHHHHHEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCEE
KVPNQFQSSLADLSIKKSFNEWQALGVRRFDGSDLPKREDMQISMVMPDGIAGRSYLIYD
CCCHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCEEEEECCCCCCCCEEEEEE
NYRGLLQWNNSDYFAISVTYLSERIKYPPII
CCCEEEEECCCCEEEEEEHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]