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The map label for this gene is def [H]

Identifier: 157962208

GI number: 157962208

Start: 2910137

End: 2910628

Strand: Reverse

Name: def [H]

Synonym: Spea_2387

Alternate gene names: 157962208

Gene position: 2910628-2910137 (Counterclockwise)

Preceding gene: 157962210

Following gene: 157962207

Centisome position: 56.25

GC content: 43.7

Gene sequence:

>492_bases
ATGGCAGTGTTAGACATTTTAACGATCCCCGATGAGCGCCTTAAGCGTAAAGCGGTTCCGGTAACAGATATTGAGTCTGT
ACAAGGCTTTATCGATGATTTGATTGAGACTATGTATGACACCGACGATGGCATTGGGTTGGCTGCAACTCAAGTTGGTA
GCTTGCATGCTATCTTGGTCATCGATCTCTCTGAGGAGCGTGATCAGCCAATGGTATTGATTAACCCTGAGATAGTAGAA
TCTCGTGGTGAATACCAAGGTGAAGAGGGCTGTTTATCGATTCCTGGGTATCGCGCTAAGGTAAATCGTCATGAAGGCGT
TAAGGTAAGCGCTTTAGATCGTCAAGGTAAAGCATTTGAAATCGATACCGATGAATTCTTAGCCATTGTGTTGCAGCATG
AAATGGATCACCTTAATGGTGTGGTGTTTACTGACCATCTTTCTATGCTAAAGCAGCAGGTTGCCCTAAAGAAAGTAAAG
AAGTACAGATAG

Upstream 100 bases:

>100_bases
GAGGTAGAAAATTAACTTAAATCCTTACTGGAGTTGCCTTTGCGCTTGATTCGGTCATAATAGCGCGAATTTTTGAGTTT
ACTTCTAGTGTGATAATAAT

Downstream 100 bases:

>100_bases
ATTCTCAATTATTAACATCTAATCTTTAGCATCTAGTTCTCGACATAAAGCTTGTCGGTGATAGATGTCGTTAATGGTTC
AAAAAAAAGCGTCCCTTACC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 3; Polypeptide deformylase 3 [H]

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MAVLDILTIPDERLKRKAVPVTDIESVQGFIDDLIETMYDTDDGIGLAATQVGSLHAILVIDLSEERDQPMVLINPEIVE
SRGEYQGEEGCLSIPGYRAKVNRHEGVKVSALDRQGKAFEIDTDEFLAIVLQHEMDHLNGVVFTDHLSMLKQQVALKKVK
KYR

Sequences:

>Translated_163_residues
MAVLDILTIPDERLKRKAVPVTDIESVQGFIDDLIETMYDTDDGIGLAATQVGSLHAILVIDLSEERDQPMVLINPEIVE
SRGEYQGEEGCLSIPGYRAKVNRHEGVKVSALDRQGKAFEIDTDEFLAIVLQHEMDHLNGVVFTDHLSMLKQQVALKKVK
KYR
>Mature_162_residues
AVLDILTIPDERLKRKAVPVTDIESVQGFIDDLIETMYDTDDGIGLAATQVGSLHAILVIDLSEERDQPMVLINPEIVES
RGEYQGEEGCLSIPGYRAKVNRHEGVKVSALDRQGKAFEIDTDEFLAIVLQHEMDHLNGVVFTDHLSMLKQQVALKKVKK
YR

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=162, Percent_Identity=56.7901234567901, Blast_Score=173, Evalue=6e-45,
Organism=Drosophila melanogaster, GI24645728, Length=159, Percent_Identity=30.188679245283, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24645726, Length=122, Percent_Identity=33.6065573770492, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 18259; Mature: 18128

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVLDILTIPDERLKRKAVPVTDIESVQGFIDDLIETMYDTDDGIGLAATQVGSLHAILV
CCEEEEEECCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEE
IDLSEERDQPMVLINPEIVESRGEYQGEEGCLSIPGYRAKVNRHEGVKVSALDRQGKAFE
EEECCCCCCCEEEECHHHHHCCCCCCCCCCCEECCCCEEECCCCCCCEEEEECCCCCEEE
IDTDEFLAIVLQHEMDHLNGVVFTDHLSMLKQQVALKKVKKYR
ECHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AVLDILTIPDERLKRKAVPVTDIESVQGFIDDLIETMYDTDDGIGLAATQVGSLHAILV
CEEEEEECCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEE
IDLSEERDQPMVLINPEIVESRGEYQGEEGCLSIPGYRAKVNRHEGVKVSALDRQGKAFE
EEECCCCCCCEEEECHHHHHCCCCCCCCCCCEECCCCEEECCCCCCCEEEEECCCCCEEE
IDTDEFLAIVLQHEMDHLNGVVFTDHLSMLKQQVALKKVKKYR
ECHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]