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The map label for this gene is prpC [H]

Identifier: 157962142

GI number: 157962142

Start: 2827508

End: 2828662

Strand: Reverse

Name: prpC [H]

Synonym: Spea_2321

Alternate gene names: 157962142

Gene position: 2828662-2827508 (Counterclockwise)

Preceding gene: 157962143

Following gene: 157962141

Centisome position: 54.66

GC content: 48.14

Gene sequence:

>1155_bases
ATGGTAGATAAGACTGAGGTTTCAAACAAGGCAGTTGATAAAAAGATTGGTGGCGCAGGCCTTCGTGGTCAAAGTGCTGG
TGAAACAGCCCTATGTACAGTGGGCAAGTCAGGTTCGGGGCTAACCTATTGTGGTTACGACGTGTCAGACTTGGCTGATA
ATGCCACATTTGAAGAGGTAGCCTACCTGTTGTTTAACGGCGAGTTACCGAATCAAACTGAGCTCGATCATTATAAAACT
GAATTGTTTGCTCAGCGCGATCTTCCACAAGCGCTTAAAGAAGTACTGCAACGTATTCCAGCCGATGCACACCCAATGGA
CGTGATGCGTACCGGTTGTTCATTTTTGGGTAACCTAGAGCCTGAGAATGACTTTACTCAGCAGAATAAAGCCTCTAATC
GTCTATTAGCCGCGTTTCCTGCCATCATGTGTTATTGGTATAAGTTCTCGCACGAGGGCGTTGAAATCGACTGTGTAACA
GACGAAGACTCTATCGGCGGTCACTTCCTGCACCTACTCAATGGCAAGGCGCCATCGGAGCTACACCGCCGCGTCATGGA
TGTGTCGTTAATTTTATATGCGGAGCATGAGTTTAACGCATCAACCTTCACCGCCCGTGTTTGTGCCTCAACCCTGTCAG
ATATGTTCTCTTGTATTACAGGCGCTATCGGTACACTACGTGGCCCGCTGCATGGCGGCGCAAACGAAGCGGCTATGGAT
ATGATCCAAAAGTTCTCATCACCACAAGATGCTAAAGAGCAGATGGCGGGCATGCTTGAGCGTAAAGAGAAGATCATGGG
CTTTGGTCACGCGATTTACCGCGAGTCAGATCCACGTAACGTTATCATCAAGGCTTGGTCTAAAAAGCTTGGGGCTGAGT
TTGGTGACAGCTCGCTTTATGATATCTCTGTAGCCTGTGAAGAGTTTATGTGGGACAGCAAGAAGTTGTTCTGTAACGCC
GACTTCTTCCACGCATCGGCTTACCATTTTATGGGTATTCCAACTAAGTTATTCACGCCAATCTTTGTCTGTTCACGTTT
AACTGGCTGGGCGGCACACGTGATGGAGCAGCGCTCTAATAACCGTATTATCCGCCCAAGTGCAGATTACACGGGTAGCG
AGCCACGCAAGGTTAACCCTATTGGTGAAAGATAG

Upstream 100 bases:

>100_bases
ATAACAAAGCGTCAGTGTGACGGAATCGCGCTGGTGTATAACGATAAGAAGCCCTGCAACCTAGTATTGATGGGGTAAAA
AAACTAGAAGGAAGAGAGCA

Downstream 100 bases:

>100_bases
ACCTTAGATTCTAGAACGCGACTTCGTCACGGCTAGAGCGTCGCTACGCTCCTGCTAGAGCCTATACAGTCAAAAGATAA
CAGCAGTACTTTTTAGGCTT

Product: 2-methylcitrate synthase/citrate synthase II

Products: NA

Alternate protein names: Citrate synthase 2; Methylcitrate synthase [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MVDKTEVSNKAVDKKIGGAGLRGQSAGETALCTVGKSGSGLTYCGYDVSDLADNATFEEVAYLLFNGELPNQTELDHYKT
ELFAQRDLPQALKEVLQRIPADAHPMDVMRTGCSFLGNLEPENDFTQQNKASNRLLAAFPAIMCYWYKFSHEGVEIDCVT
DEDSIGGHFLHLLNGKAPSELHRRVMDVSLILYAEHEFNASTFTARVCASTLSDMFSCITGAIGTLRGPLHGGANEAAMD
MIQKFSSPQDAKEQMAGMLERKEKIMGFGHAIYRESDPRNVIIKAWSKKLGAEFGDSSLYDISVACEEFMWDSKKLFCNA
DFFHASAYHFMGIPTKLFTPIFVCSRLTGWAAHVMEQRSNNRIIRPSADYTGSEPRKVNPIGER

Sequences:

>Translated_384_residues
MVDKTEVSNKAVDKKIGGAGLRGQSAGETALCTVGKSGSGLTYCGYDVSDLADNATFEEVAYLLFNGELPNQTELDHYKT
ELFAQRDLPQALKEVLQRIPADAHPMDVMRTGCSFLGNLEPENDFTQQNKASNRLLAAFPAIMCYWYKFSHEGVEIDCVT
DEDSIGGHFLHLLNGKAPSELHRRVMDVSLILYAEHEFNASTFTARVCASTLSDMFSCITGAIGTLRGPLHGGANEAAMD
MIQKFSSPQDAKEQMAGMLERKEKIMGFGHAIYRESDPRNVIIKAWSKKLGAEFGDSSLYDISVACEEFMWDSKKLFCNA
DFFHASAYHFMGIPTKLFTPIFVCSRLTGWAAHVMEQRSNNRIIRPSADYTGSEPRKVNPIGER
>Mature_384_residues
MVDKTEVSNKAVDKKIGGAGLRGQSAGETALCTVGKSGSGLTYCGYDVSDLADNATFEEVAYLLFNGELPNQTELDHYKT
ELFAQRDLPQALKEVLQRIPADAHPMDVMRTGCSFLGNLEPENDFTQQNKASNRLLAAFPAIMCYWYKFSHEGVEIDCVT
DEDSIGGHFLHLLNGKAPSELHRRVMDVSLILYAEHEFNASTFTARVCASTLSDMFSCITGAIGTLRGPLHGGANEAAMD
MIQKFSSPQDAKEQMAGMLERKEKIMGFGHAIYRESDPRNVIIKAWSKKLGAEFGDSSLYDISVACEEFMWDSKKLFCNA
DFFHASAYHFMGIPTKLFTPIFVCSRLTGWAAHVMEQRSNNRIIRPSADYTGSEPRKVNPIGER

Specific function: Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=310, Percent_Identity=22.9032258064516, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1786527, Length=388, Percent_Identity=50.7731958762887, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1786939, Length=380, Percent_Identity=32.1052631578947, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17555174, Length=304, Percent_Identity=23.6842105263158, Blast_Score=85, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6319850, Length=405, Percent_Identity=22.4691358024691, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6325257, Length=357, Percent_Identity=25.4901960784314, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324328, Length=332, Percent_Identity=21.6867469879518, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24640126, Length=308, Percent_Identity=25.3246753246753, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24640124, Length=308, Percent_Identity=25.3246753246753, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356863, Length=265, Percent_Identity=24.5283018867925, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5 [H]

Molecular weight: Translated: 42563; Mature: 42563

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDKTEVSNKAVDKKIGGAGLRGQSAGETALCTVGKSGSGLTYCGYDVSDLADNATFEEV
CCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCEEECCCHHHHHCCCCHHHH
AYLLFNGELPNQTELDHYKTELFAQRDLPQALKEVLQRIPADAHPMDVMRTGCSFLGNLE
HHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
PENDFTQQNKASNRLLAAFPAIMCYWYKFSHEGVEIDCVTDEDSIGGHFLHLLNGKAPSE
CCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCHHH
LHRRVMDVSLILYAEHEFNASTFTARVCASTLSDMFSCITGAIGTLRGPLHGGANEAAMD
HHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
MIQKFSSPQDAKEQMAGMLERKEKIMGFGHAIYRESDPRNVIIKAWSKKLGAEFGDSSLY
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEE
DISVACEEFMWDSKKLFCNADFFHASAYHFMGIPTKLFTPIFVCSRLTGWAAHVMEQRSN
EHHHHHHHHHCCCCCEEECCCHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
NRIIRPSADYTGSEPRKVNPIGER
CCEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVDKTEVSNKAVDKKIGGAGLRGQSAGETALCTVGKSGSGLTYCGYDVSDLADNATFEEV
CCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCEEECCCHHHHHCCCCHHHH
AYLLFNGELPNQTELDHYKTELFAQRDLPQALKEVLQRIPADAHPMDVMRTGCSFLGNLE
HHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
PENDFTQQNKASNRLLAAFPAIMCYWYKFSHEGVEIDCVTDEDSIGGHFLHLLNGKAPSE
CCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCHHH
LHRRVMDVSLILYAEHEFNASTFTARVCASTLSDMFSCITGAIGTLRGPLHGGANEAAMD
HHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
MIQKFSSPQDAKEQMAGMLERKEKIMGFGHAIYRESDPRNVIIKAWSKKLGAEFGDSSLY
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEE
DISVACEEFMWDSKKLFCNADFFHASAYHFMGIPTKLFTPIFVCSRLTGWAAHVMEQRSN
EHHHHHHHHHCCCCCEEECCCHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
NRIIRPSADYTGSEPRKVNPIGER
CCEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8508809; 9325432 [H]