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The map label for this gene is mii [H]

Identifier: 157962140

GI number: 157962140

Start: 2823102

End: 2824382

Strand: Reverse

Name: mii [H]

Synonym: Spea_2319

Alternate gene names: 157962140

Gene position: 2824382-2823102 (Counterclockwise)

Preceding gene: 157962141

Following gene: 157962139

Centisome position: 54.58

GC content: 51.13

Gene sequence:

>1281_bases
ATGACTAATGGCTCAAAAAGAATTCAAACGGTAAAACTTATGACCACATCAACAGACCAGAAAACAAAATTCCCGCCGCA
AATCAAAGTGCCTGCAACCTATATGCGTGGCGGCACCAGTAAAGGGGTGTTCTTTAATTTAACCGACTTGCCAACGGAAG
CGCAGGTAGCAGGCGCTGCCCGTGATGCGCTGCTGCTAAGAGTCATAGGTAGTCCAGATCCTTATGGCAAACAAACTGAC
GGCATGGGCGGCGCAACATCGAGCACCAGTAAAACAGTGATCCTTGCCAAGAGTGACAAGGCCGATCACGATGTCGATTA
CCTGTTTGGCCAAGTCGCTATCGACAGACCTTTTGTCGATTGGAGTGGCAACTGCGGCAACTTAACTGCCGCTGTGGGAT
CATTTGCAATCAGTAACGGTTTAGTCGATAGCAGCCGCATTCCTGAAAACGGTATTGCAGTGGTGCGTATTTGGCAGAAA
AACATCGGTAAAACCATTATCGCTCATGTACCCATCACCAACGGAGAAGTGCAAGAGACGGGGGAATTCGAGCTTGATGG
TGTCACGTTCCCCGCTGCCGAGGTGCAAGTTGAGTTTTTAGACCCAGCCGATGGTGATGGCGCCATGTTCCCAACAGGTA
ACTTGATTGATGACCTAATCGTGCCAACCGATGTAGTAGCTGGTGGGGTACTGCAAGCCACCATGATCAACGCAGGTATT
CCAACCGTGTTTATCAATGCGGCAGAGTTGGGTTACACAGGCTCAGAGCTGCAAGAAGCTATAAACGGCGATGTTAAAGC
GCTTGCCATGTTTGAGACGATTCGTGCCCACGGCGCAGTGCAGATGGGGCTGATTAAGCATATCGACGAAGCGGCAAACC
GTCAGCATACGCCCAAAGTGGCCATAGTAGCAGCACCAGTGGACTACACATCATCCAGTGGCAAAGCTATAGATAAAAAC
ACTATCGATTTAAATGTACGCGCACTGTCTATGGGCAAGCTTCACCATGCCATGATGGGCACCGCAGCCGTCGCTATCGG
CACCGCAGCAGCTATCCCTGGCACGCTAGTCAGCTTGGCAGCAGGTGAGGGGCAGAGTAATGCAGTTAACCCTGGCTTTG
TCACCTTTGGTCATCCATCTGGCACCCTAAAGGTCGGTGCAGAGGCAAGCCAAGTCGATGGTGACTGGCAGGTCAATAAA
GCCGTGATGAGCCGCAGTGCTCGAGTACTGATGGAAGGTTGGGTGAGAGTACCTGCGGATCCGTTAATGTCGGAAAGTTA
A

Upstream 100 bases:

>100_bases
CGCAAGTACATCATTATAGGCTCTGCGGTGGCATCCATGCCACCAAAGCTCATCTTTATAACGATATAAAACCTCACGCT
CATGTTGTTTGATTTTACAT

Downstream 100 bases:

>100_bases
GCCCAAACCTAATAAAAATAAAGAATCAAACCAAAGCAAAGAACCCAGTACAAACTGGGTTCTTTGCGTTAAAAACGATA
GAGAGCAAATTAGTCGTGAA

Product: putative AcnD-accessory protein PrpF

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTNGSKRIQTVKLMTTSTDQKTKFPPQIKVPATYMRGGTSKGVFFNLTDLPTEAQVAGAARDALLLRVIGSPDPYGKQTD
GMGGATSSTSKTVILAKSDKADHDVDYLFGQVAIDRPFVDWSGNCGNLTAAVGSFAISNGLVDSSRIPENGIAVVRIWQK
NIGKTIIAHVPITNGEVQETGEFELDGVTFPAAEVQVEFLDPADGDGAMFPTGNLIDDLIVPTDVVAGGVLQATMINAGI
PTVFINAAELGYTGSELQEAINGDVKALAMFETIRAHGAVQMGLIKHIDEAANRQHTPKVAIVAAPVDYTSSSGKAIDKN
TIDLNVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVSLAAGEGQSNAVNPGFVTFGHPSGTLKVGAEASQVDGDWQVNK
AVMSRSARVLMEGWVRVPADPLMSES

Sequences:

>Translated_426_residues
MTNGSKRIQTVKLMTTSTDQKTKFPPQIKVPATYMRGGTSKGVFFNLTDLPTEAQVAGAARDALLLRVIGSPDPYGKQTD
GMGGATSSTSKTVILAKSDKADHDVDYLFGQVAIDRPFVDWSGNCGNLTAAVGSFAISNGLVDSSRIPENGIAVVRIWQK
NIGKTIIAHVPITNGEVQETGEFELDGVTFPAAEVQVEFLDPADGDGAMFPTGNLIDDLIVPTDVVAGGVLQATMINAGI
PTVFINAAELGYTGSELQEAINGDVKALAMFETIRAHGAVQMGLIKHIDEAANRQHTPKVAIVAAPVDYTSSSGKAIDKN
TIDLNVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVSLAAGEGQSNAVNPGFVTFGHPSGTLKVGAEASQVDGDWQVNK
AVMSRSARVLMEGWVRVPADPLMSES
>Mature_425_residues
TNGSKRIQTVKLMTTSTDQKTKFPPQIKVPATYMRGGTSKGVFFNLTDLPTEAQVAGAARDALLLRVIGSPDPYGKQTDG
MGGATSSTSKTVILAKSDKADHDVDYLFGQVAIDRPFVDWSGNCGNLTAAVGSFAISNGLVDSSRIPENGIAVVRIWQKN
IGKTIIAHVPITNGEVQETGEFELDGVTFPAAEVQVEFLDPADGDGAMFPTGNLIDDLIVPTDVVAGGVLQATMINAGIP
TVFINAAELGYTGSELQEAINGDVKALAMFETIRAHGAVQMGLIKHIDEAANRQHTPKVAIVAAPVDYTSSSGKAIDKNT
IDLNVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVSLAAGEGQSNAVNPGFVTFGHPSGTLKVGAEASQVDGDWQVNKA
VMSRSARVLMEGWVRVPADPLMSES

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=391, Percent_Identity=34.0153452685422, Blast_Score=164, Evalue=9e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 44528; Mature: 44397

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNGSKRIQTVKLMTTSTDQKTKFPPQIKVPATYMRGGTSKGVFFNLTDLPTEAQVAGAA
CCCCCCEEEEEEEEEECCCCCCCCCCCEECCHHHHCCCCCCCEEEEECCCCCHHHHHHHH
RDALLLRVIGSPDPYGKQTDGMGGATSSTSKTVILAKSDKADHDVDYLFGQVAIDRPFVD
HCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHEEEEECCCCCC
WSGNCGNLTAAVGSFAISNGLVDSSRIPENGIAVVRIWQKNIGKTIIAHVPITNGEVQET
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCC
GEFELDGVTFPAAEVQVEFLDPADGDGAMFPTGNLIDDLIVPTDVVAGGVLQATMINAGI
CCEEECCEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHCCCHHHHHCCHHEEEEECCCC
PTVFINAAELGYTGSELQEAINGDVKALAMFETIRAHGAVQMGLIKHIDEAANRQHTPKV
CEEEEEHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEE
AIVAAPVDYTSSSGKAIDKNTIDLNVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVSLA
EEEEECCCCCCCCCCEECCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
AGEGQSNAVNPGFVTFGHPSGTLKVGAEASQVDGDWQVNKAVMSRSARVLMEGWVRVPAD
CCCCCCCCCCCCEEEECCCCCCEEECCCHHCCCCCCCHHHHHHHHHHHHHHHCCEECCCC
PLMSES
CCCCCC
>Mature Secondary Structure 
TNGSKRIQTVKLMTTSTDQKTKFPPQIKVPATYMRGGTSKGVFFNLTDLPTEAQVAGAA
CCCCCEEEEEEEEEECCCCCCCCCCCEECCHHHHCCCCCCCEEEEECCCCCHHHHHHHH
RDALLLRVIGSPDPYGKQTDGMGGATSSTSKTVILAKSDKADHDVDYLFGQVAIDRPFVD
HCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHEEEEECCCCCC
WSGNCGNLTAAVGSFAISNGLVDSSRIPENGIAVVRIWQKNIGKTIIAHVPITNGEVQET
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCC
GEFELDGVTFPAAEVQVEFLDPADGDGAMFPTGNLIDDLIVPTDVVAGGVLQATMINAGI
CCEEECCEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHCCCHHHHHCCHHEEEEECCCC
PTVFINAAELGYTGSELQEAINGDVKALAMFETIRAHGAVQMGLIKHIDEAANRQHTPKV
CEEEEEHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEE
AIVAAPVDYTSSSGKAIDKNTIDLNVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVSLA
EEEEECCCCCCCCCCEECCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
AGEGQSNAVNPGFVTFGHPSGTLKVGAEASQVDGDWQVNKAVMSRSARVLMEGWVRVPAD
CCCCCCCCCCCCEEEECCCCCCEEECCCHHCCCCCCCHHHHHHHHHHHHHHHCCEECCCC
PLMSES
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA