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The map label for this gene is nemA [H]

Identifier: 157961953

GI number: 157961953

Start: 2583806

End: 2584909

Strand: Direct

Name: nemA [H]

Synonym: Spea_2132

Alternate gene names: 157961953

Gene position: 2583806-2584909 (Clockwise)

Preceding gene: 157961952

Following gene: 157961954

Centisome position: 49.93

GC content: 45.56

Gene sequence:

>1104_bases
ATGATTAGTAACCAAAGCATACAAGCTAAAGAAGCGCTGCTAAGTCCATTAATAATTGGCGATATCACGCTTAATAATCG
TGTTGTGATGGCGCCGATGACCCGTAATCGCGCTAACAGTGATGGTACGCCCACGACCAGCATGGTTCAGCATTATTCTG
ATAGAGCATCCGCCGGACTCATAGTCGCAGAAGGAACTTGGCCTAGCCGCGAAGGGCAGGCATATTGCCGCCAGCCGGGT
ATTGAAACCAAAGAACATTGTCTGGCGTGGCGCCATGTCACAGATGCAGTTCATGCTAAAGGTGGAAAAATTGTGCTGCA
GATAATGCATAGTGGCCGCATTGGAAGTAAGCACATTAAAGGCGAAACCGTTAAAAGCTTCGCACCATCAGCGATTCAAG
CCGCTGGAAAGGTCTACACCGATAGCGCTGGCATGCAAGATTTTGATGTGCCGCTAGCCTTAAATACAGTGCAGGTTGCC
CAAGTGATCGCCAAGCATCGCCAAGCTGCGATTAATGCAATAGAAGCTGGGTTTGACGGTGTTGAACTTCATTGTACCAG
CGGATATTTACCCATGCAGTTCTTATGTTCTGGCTCGAATCAACGTACTGATAAATATGGTGGCAGTAGCGATAACCGTG
CAAGCTTTGCTTACGAATGTTTATTAAGCATGAGTGATGCGATTGGCGCAGGGCGGGTCGGTTTAAGGATTAACCCCGGC
AACAGTTTTAACGATTGCTCAGATGATAACCCGGCGCAAAGCCATATCAGTTTGCTAAAGCAAGTTAACGGTTTAAACCT
TGCTTATTTACATGTGATGAAATCGCCATTAGCTGAAATAGATGCGTTTGCGTTAGCACGCGAACATTTTGACGGTCCGT
TGATTTTAAATGATGGTTTCGAACCTGCAAGTGCTGCAGAAGCGGTACAAGAGCAATTGGGGCAAGCGGTCTCTTTTGCA
AGACATTTCATCGCCAATCCTGATTTAGTTTATCGAATTAATGATGACCTTCCATTATCTACATTTGATCGCAAAAGCTT
ATATACCGCCGGTGATGCTGGCTATAACAATTATCAAACTCATCAATCATTAACCACGAGTTGA

Upstream 100 bases:

>100_bases
TTTCTTGCCAGTGATGAAGCATCCTATGTCACCGGCGTTAGCTTACCTGTCGATGGTGGCCGAGCAGCTGTACTCGTTAC
GGCAATGGATTGAGGTAGTG

Downstream 100 bases:

>100_bases
AAACCGGAAAAAAAGGAAATTAATAATGAAAATACTGGTACCAATAAAACGAGTGGTCGATCATAACGTTAAAGTCAGAA
TCAAGTCTGATGGCAGTGGT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MISNQSIQAKEALLSPLIIGDITLNNRVVMAPMTRNRANSDGTPTTSMVQHYSDRASAGLIVAEGTWPSREGQAYCRQPG
IETKEHCLAWRHVTDAVHAKGGKIVLQIMHSGRIGSKHIKGETVKSFAPSAIQAAGKVYTDSAGMQDFDVPLALNTVQVA
QVIAKHRQAAINAIEAGFDGVELHCTSGYLPMQFLCSGSNQRTDKYGGSSDNRASFAYECLLSMSDAIGAGRVGLRINPG
NSFNDCSDDNPAQSHISLLKQVNGLNLAYLHVMKSPLAEIDAFALAREHFDGPLILNDGFEPASAAEAVQEQLGQAVSFA
RHFIANPDLVYRINDDLPLSTFDRKSLYTAGDAGYNNYQTHQSLTTS

Sequences:

>Translated_367_residues
MISNQSIQAKEALLSPLIIGDITLNNRVVMAPMTRNRANSDGTPTTSMVQHYSDRASAGLIVAEGTWPSREGQAYCRQPG
IETKEHCLAWRHVTDAVHAKGGKIVLQIMHSGRIGSKHIKGETVKSFAPSAIQAAGKVYTDSAGMQDFDVPLALNTVQVA
QVIAKHRQAAINAIEAGFDGVELHCTSGYLPMQFLCSGSNQRTDKYGGSSDNRASFAYECLLSMSDAIGAGRVGLRINPG
NSFNDCSDDNPAQSHISLLKQVNGLNLAYLHVMKSPLAEIDAFALAREHFDGPLILNDGFEPASAAEAVQEQLGQAVSFA
RHFIANPDLVYRINDDLPLSTFDRKSLYTAGDAGYNNYQTHQSLTTS
>Mature_367_residues
MISNQSIQAKEALLSPLIIGDITLNNRVVMAPMTRNRANSDGTPTTSMVQHYSDRASAGLIVAEGTWPSREGQAYCRQPG
IETKEHCLAWRHVTDAVHAKGGKIVLQIMHSGRIGSKHIKGETVKSFAPSAIQAAGKVYTDSAGMQDFDVPLALNTVQVA
QVIAKHRQAAINAIEAGFDGVELHCTSGYLPMQFLCSGSNQRTDKYGGSSDNRASFAYECLLSMSDAIGAGRVGLRINPG
NSFNDCSDDNPAQSHISLLKQVNGLNLAYLHVMKSPLAEIDAFALAREHFDGPLILNDGFEPASAAEAVQEQLGQAVSFA
RHFIANPDLVYRINDDLPLSTFDRKSLYTAGDAGYNNYQTHQSLTTS

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=364, Percent_Identity=37.6373626373626, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1789463, Length=339, Percent_Identity=28.6135693215339, Blast_Score=105, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17565138, Length=306, Percent_Identity=29.0849673202614, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17559802, Length=282, Percent_Identity=29.0780141843972, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI72001454, Length=344, Percent_Identity=25.2906976744186, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17540738, Length=362, Percent_Identity=25.414364640884, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17559804, Length=251, Percent_Identity=25.0996015936255, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17564188, Length=251, Percent_Identity=26.6932270916335, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=382, Percent_Identity=30.6282722513089, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6321973, Length=377, Percent_Identity=30.7692307692308, Blast_Score=164, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39610; Mature: 39610

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISNQSIQAKEALLSPLIIGDITLNNRVVMAPMTRNRANSDGTPTTSMVQHYSDRASAGL
CCCCCCCHHHHHHHCCEEEEEEEECCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCE
IVAEGTWPSREGQAYCRQPGIETKEHCLAWRHVTDAVHAKGGKIVLQIMHSGRIGSKHIK
EEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCC
GETVKSFAPSAIQAAGKVYTDSAGMQDFDVPLALNTVQVAQVIAKHRQAAINAIEAGFDG
CCHHHHHCCHHHHHCCCEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCC
VELHCTSGYLPMQFLCSGSNQRTDKYGGSSDNRASFAYECLLSMSDAIGAGRVGLRINPG
EEEEEECCCCCHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCC
NSFNDCSDDNPAQSHISLLKQVNGLNLAYLHVMKSPLAEIDAFALAREHFDGPLILNDGF
CCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
EPASAAEAVQEQLGQAVSFARHFIANPDLVYRINDDLPLSTFDRKSLYTAGDAGYNNYQT
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEECCCCCCCCCCC
HQSLTTS
CCCCCCC
>Mature Secondary Structure
MISNQSIQAKEALLSPLIIGDITLNNRVVMAPMTRNRANSDGTPTTSMVQHYSDRASAGL
CCCCCCCHHHHHHHCCEEEEEEEECCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCE
IVAEGTWPSREGQAYCRQPGIETKEHCLAWRHVTDAVHAKGGKIVLQIMHSGRIGSKHIK
EEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCC
GETVKSFAPSAIQAAGKVYTDSAGMQDFDVPLALNTVQVAQVIAKHRQAAINAIEAGFDG
CCHHHHHCCHHHHHCCCEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCC
VELHCTSGYLPMQFLCSGSNQRTDKYGGSSDNRASFAYECLLSMSDAIGAGRVGLRINPG
EEEEEECCCCCHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCC
NSFNDCSDDNPAQSHISLLKQVNGLNLAYLHVMKSPLAEIDAFALAREHFDGPLILNDGF
CCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
EPASAAEAVQEQLGQAVSFARHFIANPDLVYRINDDLPLSTFDRKSLYTAGDAGYNNYQT
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEECCCCCCCCCCC
HQSLTTS
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]