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The map label for this gene is xylJ [H]

Identifier: 157961939

GI number: 157961939

Start: 2562584

End: 2563399

Strand: Reverse

Name: xylJ [H]

Synonym: Spea_2118

Alternate gene names: 157961939

Gene position: 2563399-2562584 (Counterclockwise)

Preceding gene: 157961940

Following gene: 157961938

Centisome position: 49.54

GC content: 45.1

Gene sequence:

>816_bases
ATGATCGATAAAGTAGCTGAAACACCCAATAGCTCTGTTATTGCAATGCATGCCAAAGAGTTACTGTTAGCACTTAGCAA
AGGGACAACGATTGCTCCCTTGAGTGAGCGGACAGCGAGTCTAACCATCGATGACGCATACAAAATATCTCGATTATGCT
TGCAACAGCGTGAAGATGACGGCGAACGACTTATTGGCAAAAAAATTGGCGTAACCAGTTTGGCCGTGCAGCAAATGCTC
AATGTGCATCAGCCTGATTTTGGCTTCTTAACCAACAGCATGCATTTTAACAACGGCGATTGCATCAGCTTATCAAAACA
CAAACTCATTCAGCCTAAAGCCGAGGGTGAAATTGCCTTTTGCTTAAAGAATGATTTACAGGGACCAGGCGTTACAGCCG
AGGACGTCCTTGACGCTACGCTTTGGGTGGCTCCTTGCTTTGAAATCGTCGATTCAAGAATCGAAGATTGGCGTATAAAT
ATCGTCGATACCGTGGCAGATAATGCATCTTGTGGCGTATTTGTCATTGGTGAAACGCATACCCCACCAAGAGAGATTGA
TCTCGCCCTTGCACAAATGCAAATCCAAAAAAATGGCCAATTTGCCGGTTCAGGTCTAGGCAGTGCCGTTCAAGGCCACC
CCGCCCAAGCAGTTGCATGGTTAGCAAATACACTAGGCGAATATGGTATTCCGTTTAAAAAAGGTGAGCTTATTCTATCT
GGCGCACTGGCTCCTTTAATTGTGGCCAAGTCTGGAGATTTATTCACCATGGAAATCGAAGGTTTGGGAAGCTGCAGCAT
CAGTTTTTGTGATTAA

Upstream 100 bases:

>100_bases
GGTGAGATAAACGCACTTAAACTGCTGCTAAAGCAGATCATTAAACGCACAGATCCCGGTCTACCCGATCTGTGGTCAAA
AGTGTCTTAGGAGTCAGGTA

Downstream 100 bases:

>100_bases
TGCAATCTCAACTCTCAGCACGGAAATAATACAAAGGAAAACCGACATGACAAGTAAAATAAAATGTGCGCTGATTGGCT
CTGGCAATATTGGCACCGAC

Product: 4-oxalocrotonate decarboxylase

Products: 2-oxopent-4-enoate; H2O

Alternate protein names: HPH; 2-oxopent-4-enoate hydratase [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MIDKVAETPNSSVIAMHAKELLLALSKGTTIAPLSERTASLTIDDAYKISRLCLQQREDDGERLIGKKIGVTSLAVQQML
NVHQPDFGFLTNSMHFNNGDCISLSKHKLIQPKAEGEIAFCLKNDLQGPGVTAEDVLDATLWVAPCFEIVDSRIEDWRIN
IVDTVADNASCGVFVIGETHTPPREIDLALAQMQIQKNGQFAGSGLGSAVQGHPAQAVAWLANTLGEYGIPFKKGELILS
GALAPLIVAKSGDLFTMEIEGLGSCSISFCD

Sequences:

>Translated_271_residues
MIDKVAETPNSSVIAMHAKELLLALSKGTTIAPLSERTASLTIDDAYKISRLCLQQREDDGERLIGKKIGVTSLAVQQML
NVHQPDFGFLTNSMHFNNGDCISLSKHKLIQPKAEGEIAFCLKNDLQGPGVTAEDVLDATLWVAPCFEIVDSRIEDWRIN
IVDTVADNASCGVFVIGETHTPPREIDLALAQMQIQKNGQFAGSGLGSAVQGHPAQAVAWLANTLGEYGIPFKKGELILS
GALAPLIVAKSGDLFTMEIEGLGSCSISFCD
>Mature_271_residues
MIDKVAETPNSSVIAMHAKELLLALSKGTTIAPLSERTASLTIDDAYKISRLCLQQREDDGERLIGKKIGVTSLAVQQML
NVHQPDFGFLTNSMHFNNGDCISLSKHKLIQPKAEGEIAFCLKNDLQGPGVTAEDVLDATLWVAPCFEIVDSRIEDWRIN
IVDTVADNASCGVFVIGETHTPPREIDLALAQMQIQKNGQFAGSGLGSAVQGHPAQAVAWLANTLGEYGIPFKKGELILS
GALAPLIVAKSGDLFTMEIEGLGSCSISFCD

Specific function: Conversion of 2-hydroxypent-2,4-dienoate into 4-hydroxy- 2-oxopentanoate [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=252, Percent_Identity=40.8730158730159, Blast_Score=202, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: 4.2.1.80

Molecular weight: Translated: 29080; Mature: 29080

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDKVAETPNSSVIAMHAKELLLALSKGTTIAPLSERTASLTIDDAYKISRLCLQQREDD
CCCCCCCCCCCCEEEEEHHHHHHHHCCCCEECCCCCCCEEEEECHHHHHHHHHHHHCCCC
GERLIGKKIGVTSLAVQQMLNVHQPDFGFLTNSMHFNNGDCISLSKHKLIQPKAEGEIAF
CHHHHHHHCCHHHHHHHHHHHCCCCCCCEEECCEEECCCCEEEECCCCEECCCCCCCEEE
CLKNDLQGPGVTAEDVLDATLWVAPCFEIVDSRIEDWRINIVDTVADNASCGVFVIGETH
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCEEEEECCC
TPPREIDLALAQMQIQKNGQFAGSGLGSAVQGHPAQAVAWLANTLGEYGIPFKKGELILS
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEE
GALAPLIVAKSGDLFTMEIEGLGSCSISFCD
CCCEEEEEECCCCEEEEEECCCCCEEEEECC
>Mature Secondary Structure
MIDKVAETPNSSVIAMHAKELLLALSKGTTIAPLSERTASLTIDDAYKISRLCLQQREDD
CCCCCCCCCCCCEEEEEHHHHHHHHCCCCEECCCCCCCEEEEECHHHHHHHHHHHHCCCC
GERLIGKKIGVTSLAVQQMLNVHQPDFGFLTNSMHFNNGDCISLSKHKLIQPKAEGEIAF
CHHHHHHHCCHHHHHHHHHHHCCCCCCCEEECCEEECCCCEEEECCCCEECCCCCCCEEE
CLKNDLQGPGVTAEDVLDATLWVAPCFEIVDSRIEDWRINIVDTVADNASCGVFVIGETH
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCEEEEECCC
TPPREIDLALAQMQIQKNGQFAGSGLGSAVQGHPAQAVAWLANTLGEYGIPFKKGELILS
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEE
GALAPLIVAKSGDLFTMEIEGLGSCSISFCD
CCCEEEEEECCCCEEEEEECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxy-2-oxopentanoate

Specific reaction: 4-hydroxy-2-oxopentanoate = 2-oxopent-4-enoate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1791759 [H]