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The map label for this gene is tusD [H]

Identifier: 157961931

GI number: 157961931

Start: 2554383

End: 2554772

Strand: Reverse

Name: tusD [H]

Synonym: Spea_2110

Alternate gene names: 157961931

Gene position: 2554772-2554383 (Counterclockwise)

Preceding gene: 157961932

Following gene: 157961930

Centisome position: 49.37

GC content: 44.62

Gene sequence:

>390_bases
ATGAGCAAATTTATTATCCAGGTTAACTCAGCAGCTTACGGTTGTGCATCGAGTTACAGTGCCTACAGATTCGCTAAAGC
CGCTATAGAGAACGGCCACTCCATTGAGAAAGTCTTCTTTTATCAAGATGGCGTATTAAACACCAATCATCTCAATAGCC
CTGCTAGCGATGAGTTCGATCTTAAGCAAGCTTGGATTGATTTACACCAACGTCACGGTGTGGCTCTGGTGAACTGTGTC
TCAGCCGCGCTACGTCGTGGTGTCTTATCTGAATCTGATGCGAAAGAAAACCAATACAGCCATTGGAATATGCAACCTCC
TTTCATCATGGGTGGTTTAGGCGAATTGGTTATTGGAATTGAGACTGCCGACAGACTCGTCAGTTTTTAG

Upstream 100 bases:

>100_bases
CATCGCCCTATTACATTTAATGGGTATTGGCGGCGATGACTAATACCAATTAATTTTAATTGAGTTAAAATGGCCCTATT
ACAGGGCCATTTTTTTTACC

Downstream 100 bases:

>100_bases
GAGCATAAGTGAAAAAACTTTGTATTATTTTTAGACAGGCTCCCCACGGTACAGCACACGGCCGTGAAGGGCTCGATTTG
TCCCTTTTAAGTGCCAGTTT

Product: DsrE family protein

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein D [H]

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MSKFIIQVNSAAYGCASSYSAYRFAKAAIENGHSIEKVFFYQDGVLNTNHLNSPASDEFDLKQAWIDLHQRHGVALVNCV
SAALRRGVLSESDAKENQYSHWNMQPPFIMGGLGELVIGIETADRLVSF

Sequences:

>Translated_129_residues
MSKFIIQVNSAAYGCASSYSAYRFAKAAIENGHSIEKVFFYQDGVLNTNHLNSPASDEFDLKQAWIDLHQRHGVALVNCV
SAALRRGVLSESDAKENQYSHWNMQPPFIMGGLGELVIGIETADRLVSF
>Mature_128_residues
SKFIIQVNSAAYGCASSYSAYRFAKAAIENGHSIEKVFFYQDGVLNTNHLNSPASDEFDLKQAWIDLHQRHGVALVNCVS
AALRRGVLSESDAKENQYSHWNMQPPFIMGGLGELVIGIETADRLVSF

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Accepts sulfur from tusA and transfers it in turn to tusE [H]

COG id: COG1553

COG function: function code P; Uncharacterized conserved protein involved in intracellular sulfur reduction

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrE/tusD family [H]

Homologues:

Organism=Escherichia coli, GI1789743, Length=127, Percent_Identity=49.6062992125984, Blast_Score=127, Evalue=3e-31,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003787
- InterPro:   IPR017463 [H]

Pfam domain/function: PF02635 DrsE [H]

EC number: NA

Molecular weight: Translated: 14283; Mature: 14152

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFIIQVNSAAYGCASSYSAYRFAKAAIENGHSIEKVFFYQDGVLNTNHLNSPASDEFD
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCHH
LKQAWIDLHQRHGVALVNCVSAALRRGVLSESDAKENQYSHWNMQPPFIMGGLGELVIGI
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEE
ETADRLVSF
CCHHHHHCC
>Mature Secondary Structure 
SKFIIQVNSAAYGCASSYSAYRFAKAAIENGHSIEKVFFYQDGVLNTNHLNSPASDEFD
CEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCHH
LKQAWIDLHQRHGVALVNCVSAALRRGVLSESDAKENQYSHWNMQPPFIMGGLGELVIGI
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEE
ETADRLVSF
CCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA