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The map label for this gene is dacB [H]

Identifier: 157961903

GI number: 157961903

Start: 2522377

End: 2523774

Strand: Reverse

Name: dacB [H]

Synonym: Spea_2082

Alternate gene names: 157961903

Gene position: 2523774-2522377 (Counterclockwise)

Preceding gene: 157961904

Following gene: 157961901

Centisome position: 48.77

GC content: 42.06

Gene sequence:

>1398_bases
ATGCTGTTTGTAACCCCAGTGCTGGCTAATGATTACTTAACTAACATAGTCAAAATAATTGAACCTAAGACTTCTCAAAC
AGCCATAATTGTCAGCCCTCTTGATAACAATGCCAAAGATTTAAAGGCTGATGTAGGATTTCGTAAGGACGCAGAGCGTC
TATTTGTCCCTGCTAGTACAATGAAGTTGTTAACAGCCGTTGCAGCAACCAAGGCATTAGGCAGGGACTTTAGCTTTACA
ACACAGATAGATTCTTTTGTCGGGATTAAAAACAATCGTATTGAGGGAGATTTATTCCTCAGGTTCGATGGCGATCCAAC
ACTTACAACAAACGATCTAAGACAGTTATTTAAGCTGCTTCAGAAACAAGGCTTAAAACATATCGAGGGGAATCTCTATT
TAGTTGGCGATCAGCAAGAGACTCTACAGGCACCTGGTTGGGTTTGGGATGACTTGGGGATCTGTTATGCTGCGCCAGTT
TCCCGCTATATCCTTAATCAAAACTGTGTAAAAGCCAAGTTAGCACCAACCCTTGCCGATAATCAGTCAAAATTGACATT
TAGCCAATTCGAACCCATTTCCATCACAAATACTGCTGTTTTCGACAAAGTCGATGACATTCCTTTTTGCGAATTAAGCT
TACAACGTCACTCTAATAATCAGTTTGCGTTATCGGGTTGCTACGCTGGCCAAAAACCGTTAAAACTCTCGATAGCCATC
TCTGACCCAGCCCTTTATGCGCAAAATAGCCTAACGAATATGCTGGAGAACTTAGGGATCAGTATCAACGGCAATGTACT
GCTAACCAGTAAAAGACCTGCCAATACATTGTTGCTTGCGCAGCACAAATCTAAGCCCCTTTCCGATCTGACTGATACCA
TGTTGTTAAAATCAGATAACTTAATCGCAGACAGCCTGCTAAAGCGTCTAGGGCAGCATATTTACGGAGTGCCGGGCTCC
TTTATTAACGGAAGCACGGCAATGAAGCAGATCTTAACCGATCAAGGCATCGATCTATCCAGTGCCAATATCGTCGATGG
TTCTGGTTTATCTCGTTATAACCTACTCAGCGCCCAGCAACTTAGCCAAGTACTGCTACTTATCAAACAAGATAAACAAC
TTAACTTCTTAATCGATCAACTGCCTATCGCAGGTAAAACTGGGACACTAGCTTATCGTAGTGGCTACACAAGCTTGCCT
CTTAAAGGAATTGTGTTGGCAAAAACAGGCTCAATGATGGGAGTGGCGAATTTAGCGGGCTTTATTAAACAAGATGGACA
ATTAACCCAAGCGTTTGTGATCTTAGAAAATGGACATAGTCCTTCGGTCAAAAAAACCGAAGTCGCACCTTTTAACGTGT
TATTTTTACAGTCTATGCTTCAAAAAAGTCAACCCTAG

Upstream 100 bases:

>100_bases
GCTCGTTCAGCTCTCTTTATTTGCTGGCCACACATATCTTTACACATAGAACAAGGGCTCACTTTTGCATAAACGCGTTA
TTGCCTCCACTCTTAGCCTG

Downstream 100 bases:

>100_bases
TTAAACGGACATACAAACTAGACCAAATTATCTTCAAGTTTTAATAACAAATAAAAACGCCACTTTAAAAGTGGCGTTTT
TATTTGCAGGACTAGCTTAT

Product: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MLFVTPVLANDYLTNIVKIIEPKTSQTAIIVSPLDNNAKDLKADVGFRKDAERLFVPASTMKLLTAVAATKALGRDFSFT
TQIDSFVGIKNNRIEGDLFLRFDGDPTLTTNDLRQLFKLLQKQGLKHIEGNLYLVGDQQETLQAPGWVWDDLGICYAAPV
SRYILNQNCVKAKLAPTLADNQSKLTFSQFEPISITNTAVFDKVDDIPFCELSLQRHSNNQFALSGCYAGQKPLKLSIAI
SDPALYAQNSLTNMLENLGISINGNVLLTSKRPANTLLLAQHKSKPLSDLTDTMLLKSDNLIADSLLKRLGQHIYGVPGS
FINGSTAMKQILTDQGIDLSSANIVDGSGLSRYNLLSAQQLSQVLLLIKQDKQLNFLIDQLPIAGKTGTLAYRSGYTSLP
LKGIVLAKTGSMMGVANLAGFIKQDGQLTQAFVILENGHSPSVKKTEVAPFNVLFLQSMLQKSQP

Sequences:

>Translated_465_residues
MLFVTPVLANDYLTNIVKIIEPKTSQTAIIVSPLDNNAKDLKADVGFRKDAERLFVPASTMKLLTAVAATKALGRDFSFT
TQIDSFVGIKNNRIEGDLFLRFDGDPTLTTNDLRQLFKLLQKQGLKHIEGNLYLVGDQQETLQAPGWVWDDLGICYAAPV
SRYILNQNCVKAKLAPTLADNQSKLTFSQFEPISITNTAVFDKVDDIPFCELSLQRHSNNQFALSGCYAGQKPLKLSIAI
SDPALYAQNSLTNMLENLGISINGNVLLTSKRPANTLLLAQHKSKPLSDLTDTMLLKSDNLIADSLLKRLGQHIYGVPGS
FINGSTAMKQILTDQGIDLSSANIVDGSGLSRYNLLSAQQLSQVLLLIKQDKQLNFLIDQLPIAGKTGTLAYRSGYTSLP
LKGIVLAKTGSMMGVANLAGFIKQDGQLTQAFVILENGHSPSVKKTEVAPFNVLFLQSMLQKSQP
>Mature_465_residues
MLFVTPVLANDYLTNIVKIIEPKTSQTAIIVSPLDNNAKDLKADVGFRKDAERLFVPASTMKLLTAVAATKALGRDFSFT
TQIDSFVGIKNNRIEGDLFLRFDGDPTLTTNDLRQLFKLLQKQGLKHIEGNLYLVGDQQETLQAPGWVWDDLGICYAAPV
SRYILNQNCVKAKLAPTLADNQSKLTFSQFEPISITNTAVFDKVDDIPFCELSLQRHSNNQFALSGCYAGQKPLKLSIAI
SDPALYAQNSLTNMLENLGISINGNVLLTSKRPANTLLLAQHKSKPLSDLTDTMLLKSDNLIADSLLKRLGQHIYGVPGS
FINGSTAMKQILTDQGIDLSSANIVDGSGLSRYNLLSAQQLSQVLLLIKQDKQLNFLIDQLPIAGKTGTLAYRSGYTSLP
LKGIVLAKTGSMMGVANLAGFIKQDGQLTQAFVILENGHSPSVKKTEVAPFNVLFLQSMLQKSQP

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=403, Percent_Identity=37.7171215880893, Blast_Score=254, Evalue=9e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 50777; Mature: 50777

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFVTPVLANDYLTNIVKIIEPKTSQTAIIVSPLDNNAKDLKADVGFRKDAERLFVPAST
CEEEECHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCEEECHHH
MKLLTAVAATKALGRDFSFTTQIDSFVGIKNNRIEGDLFLRFDGDPTLTTNDLRQLFKLL
HHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCEECCEEEEEECCCCCEEHHHHHHHHHHH
QKQGLKHIEGNLYLVGDQQETLQAPGWVWDDLGICYAAPVSRYILNQNCVKAKLAPTLAD
HHCCCHHHCCCEEEECCCHHHHCCCCCEECCCCHHHHHHHHHHHHCCCCHHHHCCCCCCC
NQSKLTFSQFEPISITNTAVFDKVDDIPFCELSLQRHSNNQFALSGCYAGQKPLKLSIAI
CCCCEECCCCCCEEEECHHHHCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCEEEEEEE
SDPALYAQNSLTNMLENLGISINGNVLLTSKRPANTLLLAQHKSKPLSDLTDTMLLKSDN
CCCCHHHHHHHHHHHHHCCEEECCCEEEECCCCCCEEEEEECCCCCHHHHHHHHHCCCCC
LIADSLLKRLGQHIYGVPGSFINGSTAMKQILTDQGIDLSSANIVDGSGLSRYNLLSAQQ
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHH
LSQVLLLIKQDKQLNFLIDQLPIAGKTGTLAYRSGYTSLPLKGIVLAKTGSMMGVANLAG
HHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCHHHHHHHH
FIKQDGQLTQAFVILENGHSPSVKKTEVAPFNVLFLQSMLQKSQP
HHHCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLFVTPVLANDYLTNIVKIIEPKTSQTAIIVSPLDNNAKDLKADVGFRKDAERLFVPAST
CEEEECHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCEEECHHH
MKLLTAVAATKALGRDFSFTTQIDSFVGIKNNRIEGDLFLRFDGDPTLTTNDLRQLFKLL
HHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCEECCEEEEEECCCCCEEHHHHHHHHHHH
QKQGLKHIEGNLYLVGDQQETLQAPGWVWDDLGICYAAPVSRYILNQNCVKAKLAPTLAD
HHCCCHHHCCCEEEECCCHHHHCCCCCEECCCCHHHHHHHHHHHHCCCCHHHHCCCCCCC
NQSKLTFSQFEPISITNTAVFDKVDDIPFCELSLQRHSNNQFALSGCYAGQKPLKLSIAI
CCCCEECCCCCCEEEECHHHHCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCEEEEEEE
SDPALYAQNSLTNMLENLGISINGNVLLTSKRPANTLLLAQHKSKPLSDLTDTMLLKSDN
CCCCHHHHHHHHHHHHHCCEEECCCEEEECCCCCCEEEEEECCCCCHHHHHHHHHCCCCC
LIADSLLKRLGQHIYGVPGSFINGSTAMKQILTDQGIDLSSANIVDGSGLSRYNLLSAQQ
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHH
LSQVLLLIKQDKQLNFLIDQLPIAGKTGTLAYRSGYTSLPLKGIVLAKTGSMMGVANLAG
HHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCHHHHHHHH
FIKQDGQLTQAFVILENGHSPSVKKTEVAPFNVLFLQSMLQKSQP
HHHCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]