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The map label for this gene is pbp [H]

Identifier: 157961889

GI number: 157961889

Start: 2506347

End: 2507504

Strand: Reverse

Name: pbp [H]

Synonym: Spea_2068

Alternate gene names: 157961889

Gene position: 2507504-2506347 (Counterclockwise)

Preceding gene: 157961891

Following gene: 157961887

Centisome position: 48.46

GC content: 43.01

Gene sequence:

>1158_bases
ATGCTTTTAAAACCTTTCCTCCTTATCGGCTCCTTATCTATAGGGTCTTTGCTTACGCTTGTGCAAGTATCAGCTGCAGA
GTCTCAATATTCCGATCTCTCAAATAGTTTCAAAAAACGCTTCAACCATGAATTAAGAGAAAACAAACTTCCCGGCGGCG
CTTTCGTAATCGTTGAAGCAGATAATGTTTTAAAGTTAGGTACCTATGGAAAAAGAAGTAAAGGCAGTAATCCAAATATT
AATGCTGACACAGTCTTTAGACTCGCCTCAGTCTCTAAAACCTTTGCAGGTACCCTGGCGTCCATGTTGGTCGAAGAGGG
AAAGCTGGATTGGCAACAACCTATTGCTAGTTATTTACCGGAATTTACTTTAGCTGATCAGCAAAATGCAAAACTCATTA
ATTTAGGCCATATCATTGGTCATAGTACTGGCTTAATGCCCAATAGTTACGACAATTTGATCAACGCGAACGTCAATCTT
ACTAAAATTGTCAGCAAGTTTGATGAGCTTAGCCCTATTTGCCAACCGGGGATCTGCTATGGCTATCAAAATGTGGCGTT
TTCTTTTATCAAAGAAGCCATAGAACAACAATCGACTTTCAGCTACGCCAACTTTATACAGAAACGGATCTTTGCTCCAT
TAAGTATGAGCTCTGCCTCTGTGGGCTACGACGCGTTTCTAAATGAAAGTAACCGAGCGGAACCCCACGTCAAAACCAAA
TCAGGATTTCAGCAGGTTAAAGTTAAACCCAACTATTATCAGCTCGCCCCCGCAGCTGGTGTTAATGCGAGTATTACCGA
TCTTTCAAAATGGCTAATGGCGAATTTAGGCCAAAACCCCGAAGTAATCTCAGCAAGTATGATAGAGGATATAACCACGC
CAGGGGTTAAGACCACCAAAGAGTTACGCCGCCGAGATTGGCGCCCTTTCCTCGATAGTGCCCATTACGGTAAAGGTTGG
CGCATCTATGAGTTCAACGGACGGCCGCTTATCTATCATGCAGGATGGGTTTCAGGTTATGTCGCAGAAATATCTTACTC
ACCGGAGCTCAATATTGGTATGGCGATGTTATTGAATGGCGAATCCAGAGTCATCGCTAAGTTAAGTGCTGAGTTTTGGC
AGGATGCGTTTAAACGAGCCGACAAGCTTGGAATATAA

Upstream 100 bases:

>100_bases
GCATCAATAGAGATGTTTTTAAGTGACCATCTATTGTTAGTAGCTTAAGTCACAACAAATATACTAAACAGACCGTTATA
ACGGCACATTGGACTTGTAC

Downstream 100 bases:

>100_bases
GCGGCTGTAGCAGCCGCTATTGGTCGTTTGTTCATACCAAAGCTTAAATCATCTGAAAGTAAAAGATCCACCTTCTGAAG
AAGGTGGCTTTGCCCATCTG

Product: beta-lactamase

Products: A Substituted Beta- Amino Acid. [C]

Alternate protein names: PBP-4 [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MLLKPFLLIGSLSIGSLLTLVQVSAAESQYSDLSNSFKKRFNHELRENKLPGGAFVIVEADNVLKLGTYGKRSKGSNPNI
NADTVFRLASVSKTFAGTLASMLVEEGKLDWQQPIASYLPEFTLADQQNAKLINLGHIIGHSTGLMPNSYDNLINANVNL
TKIVSKFDELSPICQPGICYGYQNVAFSFIKEAIEQQSTFSYANFIQKRIFAPLSMSSASVGYDAFLNESNRAEPHVKTK
SGFQQVKVKPNYYQLAPAAGVNASITDLSKWLMANLGQNPEVISASMIEDITTPGVKTTKELRRRDWRPFLDSAHYGKGW
RIYEFNGRPLIYHAGWVSGYVAEISYSPELNIGMAMLLNGESRVIAKLSAEFWQDAFKRADKLGI

Sequences:

>Translated_385_residues
MLLKPFLLIGSLSIGSLLTLVQVSAAESQYSDLSNSFKKRFNHELRENKLPGGAFVIVEADNVLKLGTYGKRSKGSNPNI
NADTVFRLASVSKTFAGTLASMLVEEGKLDWQQPIASYLPEFTLADQQNAKLINLGHIIGHSTGLMPNSYDNLINANVNL
TKIVSKFDELSPICQPGICYGYQNVAFSFIKEAIEQQSTFSYANFIQKRIFAPLSMSSASVGYDAFLNESNRAEPHVKTK
SGFQQVKVKPNYYQLAPAAGVNASITDLSKWLMANLGQNPEVISASMIEDITTPGVKTTKELRRRDWRPFLDSAHYGKGW
RIYEFNGRPLIYHAGWVSGYVAEISYSPELNIGMAMLLNGESRVIAKLSAEFWQDAFKRADKLGI
>Mature_385_residues
MLLKPFLLIGSLSIGSLLTLVQVSAAESQYSDLSNSFKKRFNHELRENKLPGGAFVIVEADNVLKLGTYGKRSKGSNPNI
NADTVFRLASVSKTFAGTLASMLVEEGKLDWQQPIASYLPEFTLADQQNAKLINLGHIIGHSTGLMPNSYDNLINANVNL
TKIVSKFDELSPICQPGICYGYQNVAFSFIKEAIEQQSTFSYANFIQKRIFAPLSMSSASVGYDAFLNESNRAEPHVKTK
SGFQQVKVKPNYYQLAPAAGVNASITDLSKWLMANLGQNPEVISASMIEDITTPGVKTTKELRRRDWRPFLDSAHYGKGW
RIYEFNGRPLIYHAGWVSGYVAEISYSPELNIGMAMLLNGESRVIAKLSAEFWQDAFKRADKLGI

Specific function: Involved in cell wall biosynthesis and may also act as a sensor of external penicillins [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Escherichia coli, GI1790593, Length=381, Percent_Identity=23.0971128608924, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: 3.5.2.6 [C]

Molecular weight: Translated: 42658; Mature: 42658

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKPFLLIGSLSIGSLLTLVQVSAAESQYSDLSNSFKKRFNHELRENKLPGGAFVIVEA
CCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
DNVLKLGTYGKRSKGSNPNINADTVFRLASVSKTFAGTLASMLVEEGKLDWQQPIASYLP
CCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
EFTLADQQNAKLINLGHIIGHSTGLMPNSYDNLINANVNLTKIVSKFDELSPICQPGICY
CCEECCCCCCEEEEEHHHHHCCCCCCCCCCHHHEECCCHHHHHHHHHHHHCCCCCCCCHH
GYQNVAFSFIKEAIEQQSTFSYANFIQKRIFAPLSMSSASVGYDAFLNESNRAEPHVKTK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCC
SGFQQVKVKPNYYQLAPAAGVNASITDLSKWLMANLGQNPEVISASMIEDITTPGVKTTK
CCCEEEEECCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHH
ELRRRDWRPFLDSAHYGKGWRIYEFNGRPLIYHAGWVSGYVAEISYSPELNIGMAMLLNG
HHHHCCCCHHHCCCCCCCCEEEEEECCCEEEEECCCHHCEEEEEECCCCCCCCEEEEECC
ESRVIAKLSAEFWQDAFKRADKLGI
CCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLLKPFLLIGSLSIGSLLTLVQVSAAESQYSDLSNSFKKRFNHELRENKLPGGAFVIVEA
CCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
DNVLKLGTYGKRSKGSNPNINADTVFRLASVSKTFAGTLASMLVEEGKLDWQQPIASYLP
CCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
EFTLADQQNAKLINLGHIIGHSTGLMPNSYDNLINANVNLTKIVSKFDELSPICQPGICY
CCEECCCCCCEEEEEHHHHHCCCCCCCCCCHHHEECCCHHHHHHHHHHHHCCCCCCCCHH
GYQNVAFSFIKEAIEQQSTFSYANFIQKRIFAPLSMSSASVGYDAFLNESNRAEPHVKTK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCC
SGFQQVKVKPNYYQLAPAAGVNASITDLSKWLMANLGQNPEVISASMIEDITTPGVKTTK
CCCEEEEECCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHH
ELRRRDWRPFLDSAHYGKGWRIYEFNGRPLIYHAGWVSGYVAEISYSPELNIGMAMLLNG
HHHHCCCCHHHCCCCCCCCEEEEEECCCEEEEECCCHHCEEEEEECCCCCCCCEEEEECC
ESRVIAKLSAEFWQDAFKRADKLGI
CCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Beta-Lactam; H2O [C]

Specific reaction: A Beta-Lactam + H2O = A Substituted Beta- Amino Acid. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8440253 [H]