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The map label for this gene is ybfF [C]

Identifier: 157961875

GI number: 157961875

Start: 2485965

End: 2486723

Strand: Reverse

Name: ybfF [C]

Synonym: Spea_2054

Alternate gene names: 157961875

Gene position: 2486723-2485965 (Counterclockwise)

Preceding gene: 157961878

Following gene: 157961874

Centisome position: 48.06

GC content: 44.01

Gene sequence:

>759_bases
ATGCATCATGTGATCAGTGGCCAAGGTCCAGTAGTTATCCTCATTCATGGTTTATTTGGGGATCTTGATAACCTCAAGGC
TTTAGGCAAAGAACTCGAGGAGCAATTTACTGTTGTTCGCATTGATGTACTCAATCATGGCTGTAGCCCTCAGGTTGCCA
GCATGTGCTATGAATCACTCGCTGATGCAATGGCGAAATTGATTAGAGAACTTGACTGTAAAGAAGCTATTTTGATTGGT
CACTCCATGGGCGGCAAGATAGCCATGGCTACTGCACTCAATTATCCCCACTTGGTCAGTAAACTGGTTGTAGCCGATAT
TGCGCCAGTTGCCTACCAAAGTCGTCACGACAAAGTTTTTGCCGCGTTAGAGTCTATGCCATTAAACGATCTAAAAGATC
GCCGCCAAGCTCTTGCTCATATGCAATCATGTGGTGTTGATGACGGTACTGCACAGTTTTTATTAAAGTCTCTGACACGT
GCAGAGCAAGGTTTCAGCTGGAAAATGAACCTAGAGGGACTTAAAGCAAGCTACGCAGATATTATTGATTGGCCGACCTT
TTCAGGTAATTACCAAGGCCCCTGTCTATTTGTTCGTGGTGGTGATTCCGATTATGTTACCGCAGAGCATAGGCAAGCTA
TCATGATTCAGTTTCCAACGGTTAAAGCAAAAACTATCGAGGGAACAGGTCATTGGTTGCATGCGCAAAAACCAGCCATA
TTCAACCGAATAGTTAAAGATTTTATAACCGCCGATTGA

Upstream 100 bases:

>100_bases
ACTTTTTCAGCAGTAAACACCGATTTACCCGCCCATTGTTGGAATTTATCCTAATCCGAGGCACAATCTGCGCCAATCGA
CTCAAGTAATCGGAAAGACA

Downstream 100 bases:

>100_bases
ACCACATTTCACGCCAGAAAGGCATAAAGCATATAGCCGCTCGAAAACTTATATGGTATATTCGCGCAACTTTTTTGGCC
TAGAAAGGAATGGCAAATGC

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MHHVISGQGPVVILIHGLFGDLDNLKALGKELEEQFTVVRIDVLNHGCSPQVASMCYESLADAMAKLIRELDCKEAILIG
HSMGGKIAMATALNYPHLVSKLVVADIAPVAYQSRHDKVFAALESMPLNDLKDRRQALAHMQSCGVDDGTAQFLLKSLTR
AEQGFSWKMNLEGLKASYADIIDWPTFSGNYQGPCLFVRGGDSDYVTAEHRQAIMIQFPTVKAKTIEGTGHWLHAQKPAI
FNRIVKDFITAD

Sequences:

>Translated_252_residues
MHHVISGQGPVVILIHGLFGDLDNLKALGKELEEQFTVVRIDVLNHGCSPQVASMCYESLADAMAKLIRELDCKEAILIG
HSMGGKIAMATALNYPHLVSKLVVADIAPVAYQSRHDKVFAALESMPLNDLKDRRQALAHMQSCGVDDGTAQFLLKSLTR
AEQGFSWKMNLEGLKASYADIIDWPTFSGNYQGPCLFVRGGDSDYVTAEHRQAIMIQFPTVKAKTIEGTGHWLHAQKPAI
FNRIVKDFITAD
>Mature_252_residues
MHHVISGQGPVVILIHGLFGDLDNLKALGKELEEQFTVVRIDVLNHGCSPQVASMCYESLADAMAKLIRELDCKEAILIG
HSMGGKIAMATALNYPHLVSKLVVADIAPVAYQSRHDKVFAALESMPLNDLKDRRQALAHMQSCGVDDGTAQFLLKSLTR
AEQGFSWKMNLEGLKASYADIIDWPTFSGNYQGPCLFVRGGDSDYVTAEHRQAIMIQFPTVKAKTIEGTGHWLHAQKPAI
FNRIVKDFITAD

Specific function: Unknown

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200012, Length=249, Percent_Identity=34.5381526104418, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI23200008, Length=249, Percent_Identity=34.5381526104418, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI223941839, Length=243, Percent_Identity=28.3950617283951, Blast_Score=117, Evalue=7e-27,
Organism=Escherichia coli, GI1786902, Length=240, Percent_Identity=37.5, Blast_Score=177, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17508535, Length=254, Percent_Identity=33.4645669291339, Blast_Score=148, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17507063, Length=260, Percent_Identity=27.6923076923077, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321468, Length=265, Percent_Identity=30.9433962264151, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24640348, Length=258, Percent_Identity=27.5193798449612, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24646055, Length=257, Percent_Identity=24.124513618677, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR012908 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07819 PGAP1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 27748; Mature: 27748

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHHVISGQGPVVILIHGLFGDLDNLKALGKELEEQFTVVRIDVLNHGCSPQVASMCYESL
CCCCCCCCCCEEEEEECHHCCHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHH
ADAMAKLIRELDCKEAILIGHSMGGKIAMATALNYPHLVSKLVVADIAPVAYQSRHDKVF
HHHHHHHHHHCCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AALESMPLNDLKDRRQALAHMQSCGVDDGTAQFLLKSLTRAEQGFSWKMNLEGLKASYAD
HHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHH
IIDWPTFSGNYQGPCLFVRGGDSDYVTAEHRQAIMIQFPTVKAKTIEGTGHWLHAQKPAI
HHCCCCCCCCCCCCEEEEECCCCCCEECCCCCEEEEEECCCEEEEECCCCCEEECCCCHH
FNRIVKDFITAD
HHHHHHHHHCCC
>Mature Secondary Structure
MHHVISGQGPVVILIHGLFGDLDNLKALGKELEEQFTVVRIDVLNHGCSPQVASMCYESL
CCCCCCCCCCEEEEEECHHCCHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHH
ADAMAKLIRELDCKEAILIGHSMGGKIAMATALNYPHLVSKLVVADIAPVAYQSRHDKVF
HHHHHHHHHHCCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AALESMPLNDLKDRRQALAHMQSCGVDDGTAQFLLKSLTRAEQGFSWKMNLEGLKASYAD
HHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHH
IIDWPTFSGNYQGPCLFVRGGDSDYVTAEHRQAIMIQFPTVKAKTIEGTGHWLHAQKPAI
HHCCCCCCCCCCCCEEEEECCCCCCEECCCCCEEEEEECCCEEEEECCCCCEEECCCCHH
FNRIVKDFITAD
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]