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The map label for this gene is hydD [H]

Identifier: 157961851

GI number: 157961851

Start: 2456723

End: 2457280

Strand: Reverse

Name: hydD [H]

Synonym: Spea_2030

Alternate gene names: 157961851

Gene position: 2457280-2456723 (Counterclockwise)

Preceding gene: 157961852

Following gene: 157961850

Centisome position: 47.49

GC content: 45.34

Gene sequence:

>558_bases
ATGAAGATATTGCTACTTGGTATTGGCAATGTCCTGTACGCCGATGAGGGCATCGGCGTACATTTCGTCAATTACATCCA
AGAAAACTACCGCTTCCATCATCAAACCCATCAACTTGATCTGCTTGATGGTGGAACGCTTGCCCAAGGTCTTATTCCCA
CACTTTGCCAATACGACTATCTTATCGTTGTTGACACTGTGAATGCTAACGGTGTCGCTCCAGGTGAAGTGTATTTCTTC
GACTTCGACAAGGCCCCTTCCGAGATTGATTGGCAAGGTAGTGCCCATGAAGTTGAAATGCTACAAACCCTGATTATGAT
GGAAATGGTCGGTGATAGACCCAAAACCTTCGTGCTAGGTGTTACCCCTACGGTTCTTGAGCCTATGACGCTTGGATTAA
CCGAGCAGATCCTGGCCGCCGTGCCTTTAATGGAAAAAACGCTACTGAGCCACATGGACTCTTTAGGCGTTACTCACCAA
CGTATTGCAAACATTGATATTAACAGTCTCATTCCCGACTCTTATAAACGTGGCTTAACTGTAGATGAAGAATATTAG

Upstream 100 bases:

>100_bases
TGTGTATATGGCAGTTTGGTCTGGTATTCGCTTTAAGCATAACTCTGTTGACTCTATCGTCTCTGGTTATGATTACCATA
AGAAAAAATAAAAGGAAAAC

Downstream 100 bases:

>100_bases
ATTTGAATTTAATTGTACCCGTGAAGTCCCTTTTTACGCACACCTTTGCAATCAATACCTGCAAAACGAACAGTATGATG
TAACCATAGGAAAAAACGAT

Product: hydrogenase expression/formation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MKILLLGIGNVLYADEGIGVHFVNYIQENYRFHHQTHQLDLLDGGTLAQGLIPTLCQYDYLIVVDTVNANGVAPGEVYFF
DFDKAPSEIDWQGSAHEVEMLQTLIMMEMVGDRPKTFVLGVTPTVLEPMTLGLTEQILAAVPLMEKTLLSHMDSLGVTHQ
RIANIDINSLIPDSYKRGLTVDEEY

Sequences:

>Translated_185_residues
MKILLLGIGNVLYADEGIGVHFVNYIQENYRFHHQTHQLDLLDGGTLAQGLIPTLCQYDYLIVVDTVNANGVAPGEVYFF
DFDKAPSEIDWQGSAHEVEMLQTLIMMEMVGDRPKTFVLGVTPTVLEPMTLGLTEQILAAVPLMEKTLLSHMDSLGVTHQ
RIANIDINSLIPDSYKRGLTVDEEY
>Mature_185_residues
MKILLLGIGNVLYADEGIGVHFVNYIQENYRFHHQTHQLDLLDGGTLAQGLIPTLCQYDYLIVVDTVNANGVAPGEVYFF
DFDKAPSEIDWQGSAHEVEMLQTLIMMEMVGDRPKTFVLGVTPTVLEPMTLGLTEQILAAVPLMEKTLLSHMDSLGVTHQ
RIANIDINSLIPDSYKRGLTVDEEY

Specific function: Protease Involved In The C-Terminal Processing Of Hybc, The Large Subunit Of Hydrogenase 2. Specifically Cleaves Off A 15 Amino Acid Peptide From The C Terminus Of The Precursor Of Hybc. [C]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1789367, Length=156, Percent_Identity=32.0512820512821, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 20651; Mature: 20651

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILLLGIGNVLYADEGIGVHFVNYIQENYRFHHQTHQLDLLDGGTLAQGLIPTLCQYDY
CEEEEEECCCEEEECCCCCHHHHHHHHHHHHECCCCCEEEEECCCCHHHHHHHHHHCCCE
LIVVDTVNANGVAPGEVYFFDFDKAPSEIDWQGSAHEVEMLQTLIMMEMVGDRPKTFVLG
EEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
VTPTVLEPMTLGLTEQILAAVPLMEKTLLSHMDSLGVTHQRIANIDINSLIPDSYKRGLT
CCHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHCCCCHHHCCCC
VDEEY
CCCCC
>Mature Secondary Structure
MKILLLGIGNVLYADEGIGVHFVNYIQENYRFHHQTHQLDLLDGGTLAQGLIPTLCQYDY
CEEEEEECCCEEEECCCCCHHHHHHHHHHHHECCCCCEEEEECCCCHHHHHHHHHHCCCE
LIVVDTVNANGVAPGEVYFFDFDKAPSEIDWQGSAHEVEMLQTLIMMEMVGDRPKTFVLG
EEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
VTPTVLEPMTLGLTEQILAAVPLMEKTLLSHMDSLGVTHQRIANIDINSLIPDSYKRGLT
CCHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHCCCCHHHCCCC
VDEEY
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1587288; 9639603; 14500908 [H]