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The map label for this gene is dapE [H]

Identifier: 157961812

GI number: 157961812

Start: 2415285

End: 2416430

Strand: Reverse

Name: dapE [H]

Synonym: Spea_1991

Alternate gene names: 157961812

Gene position: 2416430-2415285 (Counterclockwise)

Preceding gene: 157961813

Following gene: 157961811

Centisome position: 46.7

GC content: 47.12

Gene sequence:

>1146_bases
ATGAGCCAACTCCAAGAATCTGCGGTACTTAGCCTAGCAAAAGATCTTATTTCTCGTCCTTCCGTGACACCTTTAGATGA
AGGCTGTCAATCGCTTATGGCTGAGCGACTGAGTCAAGCGGGTTTTGAAATCGAATCTATGGTATTTGATGATACCACCA
ATATGTGGGCTCGAAGGGGCACTCAGTCTCCAGTCTTTTGTTTTGCAGGGCATACTGATGTCGTTCCAGTTGGAGATCTC
AACCGCTGGCACACTCCGCCATTTGAGCCTGTGGTGATTGATGATTACCTTCATGGTCGCGGCGCTGCCGATATGAAAGG
CTCCTTAGCTGCAATGCTTGTTGCAACTGAGCGCTTCGTTAACAAACATCCAGACCACCAAGGTTCTATCGCCTTTCTTA
TTACCAGTGATGAAGAAGGGCCTTTCATTAATGGCACCACACGCGTTATCGATACCCTCGAAGCGCGTAATGAAAAGATC
ACTTGGTCATTAGTTGGCGAGCCTTCGTCGACCCATAAACTGGGTGATATTGTAAAAAATGGCCGCCGAGGCAGCCTCAC
CGGTAACTTAACGGTTAAAGGGATCCAAGGTCATGTTGCCTACCCTCACCTAGCTGATAATCCCATTCATAAGGCTGCTC
CTGCATTAGACGAGTTAGCTCGCATGAAATGGGATAACGGTAATGAATTCTTCCCGCCAACCAGTTTTCAAATAGCCAAT
ATCAATGGCGGTACGGGTGCCTCGAATGTGATCCCTGGTGCGTTAGAAGTGATGTTCAATTTCCGTTACTCTACCGAAGT
CACGGCTGAGATCTTGATAGAGCGCGTTTTAAACATTCTCGATGCGCACGGCTTAGACTACGATATTAGCTGGGTTTATA
ACGGATTACCTTTCTTAACCGGAGAAGGTCCATTATTGGAAGCCACAAAAGCTGCCATCAAGAAGGTAACAGGTACAGAT
ACCGATCCACAGACATCGGGTGGCACCTCTGATGGTCGATTTATCGCACCAACTGGGGCCCAAGTCATCGAGGTCGGTCC
GGTGAATGCTACGATTCATAAAGTTAACGAATGCGTTAAGGTCTCCGATCTTGAGCTACTCACCGACTGTTATGAAGCCA
TTTTGGAAAATTTACTTTGCAAGTAA

Upstream 100 bases:

>100_bases
TGGCGCTTTATCCTACCTTGATCAAGCGCCCTGTTTTGGTGTCAAACGGTCAGGTCCAAGTTGGATTCAAAGAAGCTGAC
TATCGTGTGTGGTTTAATCT

Downstream 100 bases:

>100_bases
CCCATAGCCATCTATATGGACTCAAACATCGAGGCTTAGTCGAGTGTCTAGGTCATTTTGTAGAGTCCAACACTGCCGCT
GCATTTTTGAAAATGCAGCA

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MSQLQESAVLSLAKDLISRPSVTPLDEGCQSLMAERLSQAGFEIESMVFDDTTNMWARRGTQSPVFCFAGHTDVVPVGDL
NRWHTPPFEPVVIDDYLHGRGAADMKGSLAAMLVATERFVNKHPDHQGSIAFLITSDEEGPFINGTTRVIDTLEARNEKI
TWSLVGEPSSTHKLGDIVKNGRRGSLTGNLTVKGIQGHVAYPHLADNPIHKAAPALDELARMKWDNGNEFFPPTSFQIAN
INGGTGASNVIPGALEVMFNFRYSTEVTAEILIERVLNILDAHGLDYDISWVYNGLPFLTGEGPLLEATKAAIKKVTGTD
TDPQTSGGTSDGRFIAPTGAQVIEVGPVNATIHKVNECVKVSDLELLTDCYEAILENLLCK

Sequences:

>Translated_381_residues
MSQLQESAVLSLAKDLISRPSVTPLDEGCQSLMAERLSQAGFEIESMVFDDTTNMWARRGTQSPVFCFAGHTDVVPVGDL
NRWHTPPFEPVVIDDYLHGRGAADMKGSLAAMLVATERFVNKHPDHQGSIAFLITSDEEGPFINGTTRVIDTLEARNEKI
TWSLVGEPSSTHKLGDIVKNGRRGSLTGNLTVKGIQGHVAYPHLADNPIHKAAPALDELARMKWDNGNEFFPPTSFQIAN
INGGTGASNVIPGALEVMFNFRYSTEVTAEILIERVLNILDAHGLDYDISWVYNGLPFLTGEGPLLEATKAAIKKVTGTD
TDPQTSGGTSDGRFIAPTGAQVIEVGPVNATIHKVNECVKVSDLELLTDCYEAILENLLCK
>Mature_380_residues
SQLQESAVLSLAKDLISRPSVTPLDEGCQSLMAERLSQAGFEIESMVFDDTTNMWARRGTQSPVFCFAGHTDVVPVGDLN
RWHTPPFEPVVIDDYLHGRGAADMKGSLAAMLVATERFVNKHPDHQGSIAFLITSDEEGPFINGTTRVIDTLEARNEKIT
WSLVGEPSSTHKLGDIVKNGRRGSLTGNLTVKGIQGHVAYPHLADNPIHKAAPALDELARMKWDNGNEFFPPTSFQIANI
NGGTGASNVIPGALEVMFNFRYSTEVTAEILIERVLNILDAHGLDYDISWVYNGLPFLTGEGPLLEATKAAIKKVTGTDT
DPQTSGGTSDGRFIAPTGAQVIEVGPVNATIHKVNECVKVSDLELLTDCYEAILENLLCK

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=372, Percent_Identity=59.4086021505376, Blast_Score=463, Evalue=1e-132,
Organism=Escherichia coli, GI1790395, Length=271, Percent_Identity=27.3062730627306, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789236, Length=237, Percent_Identity=24.0506329113924, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17551016, Length=264, Percent_Identity=28.030303030303, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 41314; Mature: 41183

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLQESAVLSLAKDLISRPSVTPLDEGCQSLMAERLSQAGFEIESMVFDDTTNMWARRG
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEHEEECCCHHHHHHCC
TQSPVFCFAGHTDVVPVGDLNRWHTPPFEPVVIDDYLHGRGAADMKGSLAAMLVATERFV
CCCCEEEEECCCCEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
NKHPDHQGSIAFLITSDEEGPFINGTTRVIDTLEARNEKITWSLVGEPSSTHKLGDIVKN
HCCCCCCCCEEEEEECCCCCCEECCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHC
GRRGSLTGNLTVKGIQGHVAYPHLADNPIHKAAPALDELARMKWDNGNEFFPPTSFQIAN
CCCCCEEECEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
INGGTGASNVIPGALEVMFNFRYSTEVTAEILIERVLNILDAHGLDYDISWVYNGLPFLT
CCCCCCCCCCCCHHHHHHEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEHEEECCCCEEC
GEGPLLEATKAAIKKVTGTDTDPQTSGGTSDGRFIAPTGAQVIEVGPVNATIHKVNECVK
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCEEEEECCCCHHHHHHHHHHH
VSDLELLTDCYEAILENLLCK
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQLQESAVLSLAKDLISRPSVTPLDEGCQSLMAERLSQAGFEIESMVFDDTTNMWARRG
CHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEHEEECCCHHHHHHCC
TQSPVFCFAGHTDVVPVGDLNRWHTPPFEPVVIDDYLHGRGAADMKGSLAAMLVATERFV
CCCCEEEEECCCCEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
NKHPDHQGSIAFLITSDEEGPFINGTTRVIDTLEARNEKITWSLVGEPSSTHKLGDIVKN
HCCCCCCCCEEEEEECCCCCCEECCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHC
GRRGSLTGNLTVKGIQGHVAYPHLADNPIHKAAPALDELARMKWDNGNEFFPPTSFQIAN
CCCCCEEECEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
INGGTGASNVIPGALEVMFNFRYSTEVTAEILIERVLNILDAHGLDYDISWVYNGLPFLT
CCCCCCCCCCCCHHHHHHEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEHEEECCCCEEC
GEGPLLEATKAAIKKVTGTDTDPQTSGGTSDGRFIAPTGAQVIEVGPVNATIHKVNECVK
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCEEEEECCCCHHHHHHHHHHH
VSDLELLTDCYEAILENLLCK
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA