Click here to switch to the map view.

The map label for this gene is dapA

Identifier: 157961803

GI number: 157961803

Start: 2404254

End: 2405138

Strand: Direct

Name: dapA

Synonym: Spea_1980

Alternate gene names: 157961803

Gene position: 2404254-2405138 (Clockwise)

Preceding gene: 157961800

Following gene: 157961804

Centisome position: 46.46

GC content: 44.75

Gene sequence:

>885_bases
ATGATATACGGAAGCATCGTTGCCTTAGTCACGCCAATGAATAGTGACGGCACAGTTGATTATAAAAGTCTTGAAAGTTT
AGTTGAGTACCATATCGCCCAAGGTACAGATGCCATCGTTGCTGTAGGCACAACTGGTGAATCAGCTACATTACCAGCTA
AAGAACATATTGCTGTTGTTGAGCAAACGGTAAAGTTTGCTGCGGGCAGAATTCCAATTATTGGTGGTAATGGTGCAAAT
GCCACCGCCGAAGCAGTCGAGTTAACTAAAGGTCTATCTAATGTCGGTGTAGCTGCCATGTTAGGTGTGACACCTTATTA
CAATAAGCCAACGCCAAAGGGGCTTATTGCACATTATACTGCGGTTGCGGCCAGTACCGATGTACCGCAAATCCTTTATA
ACGTTCCTGGCAGAACCGCTGTCGATATGCGCCCTGAAACCGTCGCTCAGCTTGTTGGTATTAAAAACATCATAGGTGTT
AAAGAGGCTACGGGTGATCTTTCCCGCGTAAAAGAGCTACGTAACCTATGTGGTGACGACTTCCTTCTTTATAGCGGTGA
TGATGCGACTGCAAGAGAGTTTCTCTCATTAGGTGGTAATGGCGTTATTTCTGTAGCGAACAATATCGTACCAAAACAGT
TTAAAGCCATGTGCGATGCGGCTCTTAGCGGGGATGTCTCTGCGGCACTTTCGGCTGAAGAGTCAATTAAAGAGTTGTTT
AGTGTCTTGTTTTGCGAAGCTAACCCAATCCCTGTAAAATGGGCCGTCCATCGTATGGGACTGATTAGCAACGGAACAAT
TCGTTTACCTTTGACTGAACTTTCTACCGAGTTTCATGGTCTGTTGCTAGAAGCGATGAAAAATGCCCGAATAGAGGTTA
AATAA

Upstream 100 bases:

>100_bases
TCAACTTGATGATGAATATGCTTAAGTCTTGCGTCGTAGTGCTTGTTTTTAGTGGATTGCATCAGTACCATAGCCTGTCC
TGAACCCTTGGGGAATACAC

Downstream 100 bases:

>100_bases
TAAATGCTAAAGCAAATATCACCACTGATTTTAGTTGCAGCCGTTACGGCATGTAGTACGCCAATTGACAGAAGGCAAGC
TAACGACACTGATCAGAATT

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MIYGSIVALVTPMNSDGTVDYKSLESLVEYHIAQGTDAIVAVGTTGESATLPAKEHIAVVEQTVKFAAGRIPIIGGNGAN
ATAEAVELTKGLSNVGVAAMLGVTPYYNKPTPKGLIAHYTAVAASTDVPQILYNVPGRTAVDMRPETVAQLVGIKNIIGV
KEATGDLSRVKELRNLCGDDFLLYSGDDATAREFLSLGGNGVISVANNIVPKQFKAMCDAALSGDVSAALSAEESIKELF
SVLFCEANPIPVKWAVHRMGLISNGTIRLPLTELSTEFHGLLLEAMKNARIEVK

Sequences:

>Translated_294_residues
MIYGSIVALVTPMNSDGTVDYKSLESLVEYHIAQGTDAIVAVGTTGESATLPAKEHIAVVEQTVKFAAGRIPIIGGNGAN
ATAEAVELTKGLSNVGVAAMLGVTPYYNKPTPKGLIAHYTAVAASTDVPQILYNVPGRTAVDMRPETVAQLVGIKNIIGV
KEATGDLSRVKELRNLCGDDFLLYSGDDATAREFLSLGGNGVISVANNIVPKQFKAMCDAALSGDVSAALSAEESIKELF
SVLFCEANPIPVKWAVHRMGLISNGTIRLPLTELSTEFHGLLLEAMKNARIEVK
>Mature_294_residues
MIYGSIVALVTPMNSDGTVDYKSLESLVEYHIAQGTDAIVAVGTTGESATLPAKEHIAVVEQTVKFAAGRIPIIGGNGAN
ATAEAVELTKGLSNVGVAAMLGVTPYYNKPTPKGLIAHYTAVAASTDVPQILYNVPGRTAVDMRPETVAQLVGIKNIIGV
KEATGDLSRVKELRNLCGDDFLLYSGDDATAREFLSLGGNGVISVANNIVPKQFKAMCDAALSGDVSAALSAEESIKELF
SVLFCEANPIPVKWAVHRMGLISNGTIRLPLTELSTEFHGLLLEAMKNARIEVK

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=280, Percent_Identity=26.7857142857143, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI13540533, Length=291, Percent_Identity=26.1168384879725, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1788823, Length=289, Percent_Identity=51.2110726643599, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI87082415, Length=279, Percent_Identity=27.9569892473118, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789620, Length=276, Percent_Identity=27.1739130434783, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1786463, Length=243, Percent_Identity=27.1604938271605, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_SHEPA (A8H415)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001501837.1
- ProteinModelPortal:   A8H415
- SMR:   A8H415
- STRING:   A8H415
- GeneID:   5662374
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_1980
- NMPDR:   fig|398579.3.peg.1699
- eggNOG:   COG0329
- HOGENOM:   HBG358848
- OMA:   GMYRHFK
- ProtClustDB:   PRK03170
- BioCyc:   SPEA398579:SPEA_1980-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 30984; Mature: 30984

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 161-161 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYGSIVALVTPMNSDGTVDYKSLESLVEYHIAQGTDAIVAVGTTGESATLPAKEHIAVV
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHH
EQTVKFAAGRIPIIGGNGANATAEAVELTKGLSNVGVAAMLGVTPYYNKPTPKGLIAHYT
HHHHHHHCCCCEEEECCCCCCHHHHHHHHHCHHHCCHHHHHCCCCCCCCCCCCHHHHHHH
AVAASTDVPQILYNVPGRTAVDMRPETVAQLVGIKNIIGVKEATGDLSRVKELRNLCGDD
HHHCCCCHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHCCCC
FLLYSGDDATAREFLSLGGNGVISVANNIVPKQFKAMCDAALSGDVSAALSAEESIKELF
EEEECCCCHHHHHHHHCCCCEEEEHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
SVLFCEANPIPVKWAVHRMGLISNGTIRLPLTELSTEFHGLLLEAMKNARIEVK
HHHHCCCCCCCHHHHHHHHCEECCCEEEEEHHHHHHHHHHHHHHHHHCCCEECC
>Mature Secondary Structure
MIYGSIVALVTPMNSDGTVDYKSLESLVEYHIAQGTDAIVAVGTTGESATLPAKEHIAVV
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHH
EQTVKFAAGRIPIIGGNGANATAEAVELTKGLSNVGVAAMLGVTPYYNKPTPKGLIAHYT
HHHHHHHCCCCEEEECCCCCCHHHHHHHHHCHHHCCHHHHHCCCCCCCCCCCCHHHHHHH
AVAASTDVPQILYNVPGRTAVDMRPETVAQLVGIKNIIGVKEATGDLSRVKELRNLCGDD
HHHCCCCHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHCCCC
FLLYSGDDATAREFLSLGGNGVISVANNIVPKQFKAMCDAALSGDVSAALSAEESIKELF
EEEECCCCHHHHHHHHCCCCEEEEHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
SVLFCEANPIPVKWAVHRMGLISNGTIRLPLTELSTEFHGLLLEAMKNARIEVK
HHHHCCCCCCCHHHHHHHHCEECCCEEEEEHHHHHHHHHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA