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The map label for this gene is rlmL [H]

Identifier: 157961769

GI number: 157961769

Start: 2366975

End: 2369110

Strand: Direct

Name: rlmL [H]

Synonym: Spea_1946

Alternate gene names: 157961769

Gene position: 2366975-2369110 (Clockwise)

Preceding gene: 157961767

Following gene: 157961770

Centisome position: 45.74

GC content: 42.04

Gene sequence:

>2136_bases
ATGTTAAATTTTTTTGCTGCAGCTCCGCGGGGCTATGAATACGCGTTATCACTTGAGCTAGCCGAGCTAGGCGCTTCTGA
AATTAAAGAAAGTGTTGCCGGGGTTTATTTTTCCGCTCCGTTGGAGCTTGGATATCGTATAACGCTGTGGTCGCGTTTAG
CGAGCAGAATCATTTTTGTCATTTACAAGGGACCTTGTGAATCTCCTGAGCAACTGTATAACGCAGCCTATGGTATTGAC
TGGCAGATGCAGTTTTCGAACCGCAGTACATTTAGCATCGATTTCCACGGTCTAGGTGGCTTCATAAAGAACACCCAATT
TGGTGCGTTAAAAATCAAAGATGCGATTGTTGATCGTTTTAGAGATGATGACTTTTCTCGTCCAAATGTTGAGAGAGTGG
ATGCAGATTTTAGAATCGATGCGCACTTTAGACGTGGCGAGATCACTATTGGTATCAATTTCTCTGGCCCAGCACTGCAT
AAGCGTGGTTATCGCTCTACAACGGGTGAGGCGCCACTAAAAGAAAACTTAGCAGCTAATATGCTTGTTCGTAGTGGTTG
GCAGGCTGATAAAGTTGATTTGCTTGATCCTTTCTGTGGTAGCGGAACTATTTTGATTGAAGCGGCCATGATGGCTTGTG
ATATCGCGCCAGGTATTCACCGTGAACGCTTTGGTTTTGACCATTGGTTACCTCACAACAAGAAAGTATGGCAAGAGCTA
TTAAATGAGGCTCAAGCCCGTGCATCTATCGGTAAAACCCGTTGCGAAATTAAGTTCTTTGGCTCAGATATTGACTCTCG
CTTAGTTGCGTTAGCAAAGCGTAATGCAGAAAATGCGGGCGTACTTGAGTTGATTGACTTCAGCGTTGGCAATGCATTAA
CTATTGATGTGCCGGTAGAGTCTGGCTTCTTGATCTCTAACCCGCCATACGGTGAGCGCTTAGGCAACGTAACCTCATTG
TTGCAACTGTATTATCAGTTAGGTGATAAGTTTAAGGCTGAGTTTGGTGGTTGGAGTATTGCTATTTTAAATAGTGATAT
TGAACTGTTATCAGCATTGAAACTAAAAGCTGACAAGCAGATGAAGATGAATAACGGTGCACTTGAGTGTGCGTTTAACC
TTTATACGGTTCATGCTGAAAATACACGCCGCGTCGATCCAGCAAATATCAAAATTGAAGGCGATGTCAGCGATATCGCC
GTGCCGTTTGCTAACCGTATTAAAAAGAACTTTAAACAGTTAGAAAAGTGGGCTAAGAAAGAGGGCATCGATAGTTACCG
ACTATATGATGCCGACTTACCGGAATACAATGTGGCAGTAGATAAGTATCTTGATTACGTTGTGATTCAAGAGTACTCCG
CGCCATCGCAAATTCCAGAAGCAGTGACTAAGCGTCGTCTTACAGACGTTCTAATTTCTTTACCTAGCGCAATTGGTATA
GACCCGAACCATATTATTTTGAAAACCCGTGAACGCAAGAAGGGTACCAATCAGTATGAGAAGTTAGTTGCCAACAAACT
TGAACTTATTACCACGGAATACGGTGCTAAGTTTAAGTTAAACCTAAAAGAATACTTAGATACTGGACTGTTTCTCGATC
ACCGTTTAACACGTAAGCTTGTTGGTGAAAAATCAAAAGGTCGAAGTGTTTGTAATCTATTCTCTTATACGGGTTCTGCG
TCAGTACATGCAGCACTTGGTGGGGCGACTTCAGTAACAACTGTTGATATGTCAAATACTTATATCGATTGGGCAAAAGA
AAACTTTGCATTAAATGGCTTAAATAGCGATAAGTATCAGTTCGTTCAAGCTAACTGCCTACAGTGGATGAAAAGGACTC
ATGATAGATTTGACCTTATTTTCATCGATCCACCGACTTTCTCTAACTCAAAGCGTATGGAAGACTCGTTTGACGTAGAA
CGCGATCATGTCGCTATGTTAAGTGATGTGTTTAAGCTGCTAAATCCTGGCGGAGAAATCATTTTCTCTAACAACAAACG
TAAATTCAAGATGGAAATTGCGGCTTTGGAAGCACAAGGTATGTCGATTAAAAACATCGATAACCAAACACTGCCACTGG
ATTTCAAGCGCAACCCTCACATACATAATACTTGGTTAATTACCCATGCAGGCTAA

Upstream 100 bases:

>100_bases
ACTGGCTTTTAATTAGGCTTTAACCAATAACTTTTATCTTTTGGCGGCGCTAGCAGGTATAATGCCTAACTAATTTTTAC
TGTGTGTCGACTATCTATCA

Downstream 100 bases:

>100_bases
GGCCTCGGTTATTTTATATCACACAGATGCGTGTCATCTGTGTGAGTTAGCAGCTGAGTTGCTCATACAGATTGGTGTTA
AATATGATGCCTTGGATATC

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 711; Mature: 711

Protein sequence:

>711_residues
MLNFFAAAPRGYEYALSLELAELGASEIKESVAGVYFSAPLELGYRITLWSRLASRIIFVIYKGPCESPEQLYNAAYGID
WQMQFSNRSTFSIDFHGLGGFIKNTQFGALKIKDAIVDRFRDDDFSRPNVERVDADFRIDAHFRRGEITIGINFSGPALH
KRGYRSTTGEAPLKENLAANMLVRSGWQADKVDLLDPFCGSGTILIEAAMMACDIAPGIHRERFGFDHWLPHNKKVWQEL
LNEAQARASIGKTRCEIKFFGSDIDSRLVALAKRNAENAGVLELIDFSVGNALTIDVPVESGFLISNPPYGERLGNVTSL
LQLYYQLGDKFKAEFGGWSIAILNSDIELLSALKLKADKQMKMNNGALECAFNLYTVHAENTRRVDPANIKIEGDVSDIA
VPFANRIKKNFKQLEKWAKKEGIDSYRLYDADLPEYNVAVDKYLDYVVIQEYSAPSQIPEAVTKRRLTDVLISLPSAIGI
DPNHIILKTRERKKGTNQYEKLVANKLELITTEYGAKFKLNLKEYLDTGLFLDHRLTRKLVGEKSKGRSVCNLFSYTGSA
SVHAALGGATSVTTVDMSNTYIDWAKENFALNGLNSDKYQFVQANCLQWMKRTHDRFDLIFIDPPTFSNSKRMEDSFDVE
RDHVAMLSDVFKLLNPGGEIIFSNNKRKFKMEIAALEAQGMSIKNIDNQTLPLDFKRNPHIHNTWLITHAG

Sequences:

>Translated_711_residues
MLNFFAAAPRGYEYALSLELAELGASEIKESVAGVYFSAPLELGYRITLWSRLASRIIFVIYKGPCESPEQLYNAAYGID
WQMQFSNRSTFSIDFHGLGGFIKNTQFGALKIKDAIVDRFRDDDFSRPNVERVDADFRIDAHFRRGEITIGINFSGPALH
KRGYRSTTGEAPLKENLAANMLVRSGWQADKVDLLDPFCGSGTILIEAAMMACDIAPGIHRERFGFDHWLPHNKKVWQEL
LNEAQARASIGKTRCEIKFFGSDIDSRLVALAKRNAENAGVLELIDFSVGNALTIDVPVESGFLISNPPYGERLGNVTSL
LQLYYQLGDKFKAEFGGWSIAILNSDIELLSALKLKADKQMKMNNGALECAFNLYTVHAENTRRVDPANIKIEGDVSDIA
VPFANRIKKNFKQLEKWAKKEGIDSYRLYDADLPEYNVAVDKYLDYVVIQEYSAPSQIPEAVTKRRLTDVLISLPSAIGI
DPNHIILKTRERKKGTNQYEKLVANKLELITTEYGAKFKLNLKEYLDTGLFLDHRLTRKLVGEKSKGRSVCNLFSYTGSA
SVHAALGGATSVTTVDMSNTYIDWAKENFALNGLNSDKYQFVQANCLQWMKRTHDRFDLIFIDPPTFSNSKRMEDSFDVE
RDHVAMLSDVFKLLNPGGEIIFSNNKRKFKMEIAALEAQGMSIKNIDNQTLPLDFKRNPHIHNTWLITHAG
>Mature_711_residues
MLNFFAAAPRGYEYALSLELAELGASEIKESVAGVYFSAPLELGYRITLWSRLASRIIFVIYKGPCESPEQLYNAAYGID
WQMQFSNRSTFSIDFHGLGGFIKNTQFGALKIKDAIVDRFRDDDFSRPNVERVDADFRIDAHFRRGEITIGINFSGPALH
KRGYRSTTGEAPLKENLAANMLVRSGWQADKVDLLDPFCGSGTILIEAAMMACDIAPGIHRERFGFDHWLPHNKKVWQEL
LNEAQARASIGKTRCEIKFFGSDIDSRLVALAKRNAENAGVLELIDFSVGNALTIDVPVESGFLISNPPYGERLGNVTSL
LQLYYQLGDKFKAEFGGWSIAILNSDIELLSALKLKADKQMKMNNGALECAFNLYTVHAENTRRVDPANIKIEGDVSDIA
VPFANRIKKNFKQLEKWAKKEGIDSYRLYDADLPEYNVAVDKYLDYVVIQEYSAPSQIPEAVTKRRLTDVLISLPSAIGI
DPNHIILKTRERKKGTNQYEKLVANKLELITTEYGAKFKLNLKEYLDTGLFLDHRLTRKLVGEKSKGRSVCNLFSYTGSA
SVHAALGGATSVTTVDMSNTYIDWAKENFALNGLNSDKYQFVQANCLQWMKRTHDRFDLIFIDPPTFSNSKRMEDSFDVE
RDHVAMLSDVFKLLNPGGEIIFSNNKRKFKMEIAALEAQGMSIKNIDNQTLPLDFKRNPHIHNTWLITHAG

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=713, Percent_Identity=48.8078541374474, Blast_Score=691, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=267, Percent_Identity=29.5880149812734, Blast_Score=105, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 80096; Mature: 80096

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNFFAAAPRGYEYALSLELAELGASEIKESVAGVYFSAPLELGYRITLWSRLASRIIFV
CCCCEECCCCCCEEEEEEEHHHCCHHHHHHHHCCEEEECCHHCCEEEEHHHHHHCCEEEE
IYKGPCESPEQLYNAAYGIDWQMQFSNRSTFSIDFHGLGGFIKNTQFGALKIKDAIVDRF
EECCCCCCHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCHHCCCCCCEEEEHHHHHHHH
RDDDFSRPNVERVDADFRIDAHFRRGEITIGINFSGPALHKRGYRSTTGEAPLKENLAAN
CCCCCCCCCHHHCCCCEEEEEEEECCEEEEEEECCCCHHHCCCCCCCCCCCCHHHHHHHH
MLVRSGWQADKVDLLDPFCGSGTILIEAAMMACDIAPGIHRERFGFDHWLPHNKKVWQEL
HHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHH
LNEAQARASIGKTRCEIKFFGSDIDSRLVALAKRNAENAGVLELIDFSVGNALTIDVPVE
HHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEECC
SGFLISNPPYGERLGNVTSLLQLYYQLGDKFKAEFGGWSIAILNSDIELLSALKLKADKQ
CCEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCCHHHHHHHHHCCCCC
MKMNNGALECAFNLYTVHAENTRRVDPANIKIEGDVSDIAVPFANRIKKNFKQLEKWAKK
EECCCCCEEEEEEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
EGIDSYRLYDADLPEYNVAVDKYLDYVVIQEYSAPSQIPEAVTKRRLTDVLISLPSAIGI
CCCCCEEEEECCCCCCCHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHCCC
DPNHIILKTRERKKGTNQYEKLVANKLELITTEYGAKFKLNLKEYLDTGLFLDHRLTRKL
CCCEEEEEECHHCCCCHHHHHHHHHHHHEEEECCCCEEEEEHHHHHCCCCHHHHHHHHHH
VGEKSKGRSVCNLFSYTGSASVHAALGGATSVTTVDMSNTYIDWAKENFALNGLNSDKYQ
HCCCCCCCHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCEEEECCCCEEEECCCCCHHH
FVQANCLQWMKRTHDRFDLIFIDPPTFSNSKRMEDSFDVERDHVAMLSDVFKLLNPGGEI
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCE
IFSNNKRKFKMEIAALEAQGMSIKNIDNQTLPLDFKRNPHIHNTWLITHAG
EECCCCEEEEEEEEEEECCCCEEECCCCCCCCEECCCCCCCEEEEEEEECC
>Mature Secondary Structure
MLNFFAAAPRGYEYALSLELAELGASEIKESVAGVYFSAPLELGYRITLWSRLASRIIFV
CCCCEECCCCCCEEEEEEEHHHCCHHHHHHHHCCEEEECCHHCCEEEEHHHHHHCCEEEE
IYKGPCESPEQLYNAAYGIDWQMQFSNRSTFSIDFHGLGGFIKNTQFGALKIKDAIVDRF
EECCCCCCHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCHHCCCCCCEEEEHHHHHHHH
RDDDFSRPNVERVDADFRIDAHFRRGEITIGINFSGPALHKRGYRSTTGEAPLKENLAAN
CCCCCCCCCHHHCCCCEEEEEEEECCEEEEEEECCCCHHHCCCCCCCCCCCCHHHHHHHH
MLVRSGWQADKVDLLDPFCGSGTILIEAAMMACDIAPGIHRERFGFDHWLPHNKKVWQEL
HHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHH
LNEAQARASIGKTRCEIKFFGSDIDSRLVALAKRNAENAGVLELIDFSVGNALTIDVPVE
HHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEECC
SGFLISNPPYGERLGNVTSLLQLYYQLGDKFKAEFGGWSIAILNSDIELLSALKLKADKQ
CCEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCCHHHHHHHHHCCCCC
MKMNNGALECAFNLYTVHAENTRRVDPANIKIEGDVSDIAVPFANRIKKNFKQLEKWAKK
EECCCCCEEEEEEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
EGIDSYRLYDADLPEYNVAVDKYLDYVVIQEYSAPSQIPEAVTKRRLTDVLISLPSAIGI
CCCCCEEEEECCCCCCCHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHCCC
DPNHIILKTRERKKGTNQYEKLVANKLELITTEYGAKFKLNLKEYLDTGLFLDHRLTRKL
CCCEEEEEECHHCCCCHHHHHHHHHHHHEEEECCCCEEEEEHHHHHCCCCHHHHHHHHHH
VGEKSKGRSVCNLFSYTGSASVHAALGGATSVTTVDMSNTYIDWAKENFALNGLNSDKYQ
HCCCCCCCHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCEEEECCCCEEEECCCCCHHH
FVQANCLQWMKRTHDRFDLIFIDPPTFSNSKRMEDSFDVERDHVAMLSDVFKLLNPGGEI
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCE
IFSNNKRKFKMEIAALEAQGMSIKNIDNQTLPLDFKRNPHIHNTWLITHAG
EECCCCEEEEEEEEEEECCCCEEECCCCCCCCEECCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA