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The map label for this gene is mepA [H]

Identifier: 157961554

GI number: 157961554

Start: 2104951

End: 2106057

Strand: Reverse

Name: mepA [H]

Synonym: Spea_1730

Alternate gene names: 157961554

Gene position: 2106057-2104951 (Counterclockwise)

Preceding gene: 157961555

Following gene: 157961553

Centisome position: 40.7

GC content: 49.41

Gene sequence:

>1107_bases
ATGAAACACCTTTCTAAACCTTGGTTATTAACCACGACACTAGTATTAACGACACCCGTTCTCTTTGGCTGTGGCAACGA
GCCGGAAGAGGAAGCCAAAAACGATGTTGTCAGACCGGCAAAAATAGCCACTGTAGTTAGCGCAGCTGGCAGTGTACAGC
GTAGTTTTCCCGCTCAGGTATCGGCCAACGCCAGTACCTCTTTGGCATTTAGAGTGCCAGGACAGATCACTAAGCGTTAT
GTGACCGAAGGAAAACGTGTTGAAGCAGGTACGCTCATTGCAGAACTCGATCCCACCGATTTCAATATCCATCTCGATGA
TGCCCGTGCTAAGCACGAACTCGCTGTGGCTCAGCATGATCGTAATACAACATTAGTGCCTAAGGGGCTAGCAACAAAGG
CCGAATTCGATACCTCTCGCGCCGAGATGTTGATGGCTAAAGCCAACCTTGATAGAGCCAGCCAGAACCTTAAGTACACA
AAGATCTACGCTCCCTATGATGGCGTCGTTGCACAAATTCACAGTGAAGATCACGACCATGTTGCGGCAACTCAACCTAT
CGTTGAGTTTCAAAATGACAAGGTTAGTGATATCCAATTCGATCTGCCAGAAAAGCTACTAAAGCAGTTTGATCCAGATA
AGTTTAGTGAACTCTCCACCCAGGTCATCTTAGATAGCTACCCAGAAAAACCGCTCACCGCCACGTTTAAAGAGATGCGC
AAATCCACCAGCTCAGGCGCCTTGAGTTTTAGAGTCACGCTCTCAGTCATGGCCAAAGACGGTATGCGAGTGCTACCGGG
GATGAGCGCGAAGGTTCAAACAGTACTTGCTGGCGATCAACTGGGCAATAGCGAACTTGTTATTGCACCAGTCGGGGCAA
TCTTCAGCCAAGAGACCAGCAGCCTCAGTGACGGCAAGGAGTTTGTCTGGGTACTCGATAGTGAAAACAGAACTCACCTT
AGAGAGGTCAACGTCACCCGTCTGCTGACATCGGGGGCGATAATCAATTCCGGCCTAGAGGTCGGTGACAGGGTCATCGC
TGCAGGCACCAACCATATCGTCTCTAACCAGCAGGTCAAAGAACTGCAACGTGAGCGAGGCTTATAA

Upstream 100 bases:

>100_bases
TGCTGATGGCGACTTCCGTCTAGAGTACAACCAGCAGTTTTAGACAATACGACTACGCGTAACTTATGCCCTCCCCCTAA
TAAAAGCTTGTGGAGTATCT

Downstream 100 bases:

>100_bases
TGGCTAATCACACACAAACAACCGAGAAACCTGATGATATCAATGCCGAGTGCGAGGGCGGGGGCATCGCCGGTTACTCG
ATGCGTAACTCTGTGGTTAG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKHLSKPWLLTTTLVLTTPVLFGCGNEPEEEAKNDVVRPAKIATVVSAAGSVQRSFPAQVSANASTSLAFRVPGQITKRY
VTEGKRVEAGTLIAELDPTDFNIHLDDARAKHELAVAQHDRNTTLVPKGLATKAEFDTSRAEMLMAKANLDRASQNLKYT
KIYAPYDGVVAQIHSEDHDHVAATQPIVEFQNDKVSDIQFDLPEKLLKQFDPDKFSELSTQVILDSYPEKPLTATFKEMR
KSTSSGALSFRVTLSVMAKDGMRVLPGMSAKVQTVLAGDQLGNSELVIAPVGAIFSQETSSLSDGKEFVWVLDSENRTHL
REVNVTRLLTSGAIINSGLEVGDRVIAAGTNHIVSNQQVKELQRERGL

Sequences:

>Translated_368_residues
MKHLSKPWLLTTTLVLTTPVLFGCGNEPEEEAKNDVVRPAKIATVVSAAGSVQRSFPAQVSANASTSLAFRVPGQITKRY
VTEGKRVEAGTLIAELDPTDFNIHLDDARAKHELAVAQHDRNTTLVPKGLATKAEFDTSRAEMLMAKANLDRASQNLKYT
KIYAPYDGVVAQIHSEDHDHVAATQPIVEFQNDKVSDIQFDLPEKLLKQFDPDKFSELSTQVILDSYPEKPLTATFKEMR
KSTSSGALSFRVTLSVMAKDGMRVLPGMSAKVQTVLAGDQLGNSELVIAPVGAIFSQETSSLSDGKEFVWVLDSENRTHL
REVNVTRLLTSGAIINSGLEVGDRVIAAGTNHIVSNQQVKELQRERGL
>Mature_368_residues
MKHLSKPWLLTTTLVLTTPVLFGCGNEPEEEAKNDVVRPAKIATVVSAAGSVQRSFPAQVSANASTSLAFRVPGQITKRY
VTEGKRVEAGTLIAELDPTDFNIHLDDARAKHELAVAQHDRNTTLVPKGLATKAEFDTSRAEMLMAKANLDRASQNLKYT
KIYAPYDGVVAQIHSEDHDHVAATQPIVEFQNDKVSDIQFDLPEKLLKQFDPDKFSELSTQVILDSYPEKPLTATFKEMR
KSTSSGALSFRVTLSVMAKDGMRVLPGMSAKVQTVLAGDQLGNSELVIAPVGAIFSQETSSLSDGKEFVWVLDSENRTHL
REVNVTRLLTSGAIINSGLEVGDRVIAAGTNHIVSNQQVKELQRERGL

Specific function: The periplasmic linker protein component of an organic solvent and antibiotic efflux pump; confers resistance to toluene, hexane, p-xylene, ampicillin, penicillin G, erythromycin, novobiocin and tetracycline [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=343, Percent_Identity=26.530612244898, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789929, Length=374, Percent_Identity=23.5294117647059, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1789665, Length=398, Percent_Identity=22.8643216080402, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40222; Mature: 40222

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLSKPWLLTTTLVLTTPVLFGCGNEPEEEAKNDVVRPAKIATVVSAAGSVQRSFPAQV
CCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEE
SANASTSLAFRVPGQITKRYVTEGKRVEAGTLIAELDPTDFNIHLDDARAKHELAVAQHD
CCCCCCEEEEECCHHHHHHHHCCCCEEECCEEEEEECCCCEEEEECCCCCHHHHHHEECC
RNTTLVPKGLATKAEFDTSRAEMLMAKANLDRASQNLKYTKIYAPYDGVVAQIHSEDHDH
CCCEECCCCCCCCCCCCCHHHHHHHHHHCHHHHHCCCEEEEEECCCCCEEEEECCCCCCC
VAATQPIVEFQNDKVSDIQFDLPEKLLKQFDPDKFSELSTQVILDSYPEKPLTATFKEMR
CHHCCCHHHCCCCCCCCEEECCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
KSTSSGALSFRVTLSVMAKDGMRVLPGMSAKVQTVLAGDQLGNSELVIAPVGAIFSQETS
CCCCCCCEEEEEEEEEEECCCCEECCCCCCEEEEEEECCCCCCCCEEEEEHHHHHHCCCC
SLSDGKEFVWVLDSENRTHLREVNVTRLLTSGAIINSGLEVGDRVIAAGTNHIVSNQQVK
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCHHHCCCHHCCEEEECCCCCCCCCHHHH
ELQRERGL
HHHHHCCC
>Mature Secondary Structure
MKHLSKPWLLTTTLVLTTPVLFGCGNEPEEEAKNDVVRPAKIATVVSAAGSVQRSFPAQV
CCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEE
SANASTSLAFRVPGQITKRYVTEGKRVEAGTLIAELDPTDFNIHLDDARAKHELAVAQHD
CCCCCCEEEEECCHHHHHHHHCCCCEEECCEEEEEECCCCEEEEECCCCCHHHHHHEECC
RNTTLVPKGLATKAEFDTSRAEMLMAKANLDRASQNLKYTKIYAPYDGVVAQIHSEDHDH
CCCEECCCCCCCCCCCCCHHHHHHHHHHCHHHHHCCCEEEEEECCCCCEEEEECCCCCCC
VAATQPIVEFQNDKVSDIQFDLPEKLLKQFDPDKFSELSTQVILDSYPEKPLTATFKEMR
CHHCCCHHHCCCCCCCCEEECCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
KSTSSGALSFRVTLSVMAKDGMRVLPGMSAKVQTVLAGDQLGNSELVIAPVGAIFSQETS
CCCCCCCEEEEEEEEEEECCCCEECCCCCCEEEEEEECCCCCCCCEEEEEHHHHHHCCCC
SLSDGKEFVWVLDSENRTHLREVNVTRLLTSGAIINSGLEVGDRVIAAGTNHIVSNQQVK
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCHHHCCCHHCCEEEECCCCCCCCCHHHH
ELQRERGL
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9827328 [H]