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The map label for this gene is rpfG [H]

Identifier: 157961548

GI number: 157961548

Start: 2094730

End: 2095989

Strand: Reverse

Name: rpfG [H]

Synonym: Spea_1724

Alternate gene names: 157961548

Gene position: 2095989-2094730 (Counterclockwise)

Preceding gene: 157961549

Following gene: 157961547

Centisome position: 40.51

GC content: 42.46

Gene sequence:

>1260_bases
ATGACAAATAGTTGTAAAGTCGCTGTAGGACAGCTGCAAGTTGGGAACTTTGTTAGGTTACCCATAGCATGGAAAGACCA
TCCATTCATATTCAATAGCTTCAAAATTAAGCAGCAGACTCAAATTGAACTGATAAAGAATCTCGGGATCGAGTTTGTCT
ACGTCCGTCCAGAGCGTAGCGATACTCAGCCGCTTTCAAGCATCGAAGCGAAACAGCAAAAACCACAAATTATAGATAAC
AACCTCGAACAACTATCTGGTGAATTAAACCAGCAAAAAGCCAAAAGCATTGATGCACAGCAATCCTTAAGACGTCGCTT
ACAAAAAACCGAGAAGCACTTCATTCGTAATGTTGCCATGATGCGTAATCTCACCAGTAAATTACGTAACCGACCACTCG
ATGCCGTTAATGAAGCCAAAGAACTCATTCATGGCGTTTGTAATCAACTACTCACCGATGACAAGTTAGTGCTTCATCTT
ATGGCCGATGCCAAGAGTGATGAAGGAATTTATTACCATTCTCTTAACGTCGCAGTACTTTCTATGCTAATTGCCAAAGA
ACTTGGCTGGAGTCGTAAAGAAATTGAATTGGTCGGCATGGGTGCGCTATTTCACGACATAGGCAAGCTTAAGATCCCAA
CCCAGTTACTGCGTAAAACGACCCCGTTTACTCACTCTGAACAGATATATTTTAACCAGCATCCAGCAATGGGGGCAGAG
CTTATTAATATTACAGATAATTTCCCCCAACAAGCGCTGCCTATCATCTTAAACCACCATGAATTTCTAGATGGTTCAGG
CACACCTAAGGGGCTGTTAGAAAAAGATTTAGATAAGTTAAGCCAATTAGTCTGTGCGATTAATACCTACGACAACCTAT
GCCATTCCGACAGGAGCAGGAAAGCCAGAACACCATACTCTGCACTTGGCTATCTGTATAAAAACTATCAGAGTCGGCTT
AATCAGCAAGTAATAAATCGTTTAGTCAAAATGCTAGGAGTTTACCCTCCAGGCAGCGTGGTTGAGCTATCGTCGGGACA
ATTTGCTATGGTGATGTCGGTTAATTTAAGCAAACTTCTGTTACCAAGAGTGCTAGTTTACGACCCTAACGTTCCGAAGG
AACAAGCGCCGATTGTAGACTTAGCGAGTGAAGGGCTGACTATTATTAGATGTTTACCCTTAGCTGCGCTGCCAGAGAAA
GTGCTGAAATACCTCAACCCAAGAGAAAAGATCAGCTACTTCTTTGGAGCCGAGAAGTAA

Upstream 100 bases:

>100_bases
TTAACGGGTTTGCAACATCTGTAAATTTATCAGTTAAGTGATTGTAAATCATATAGCTTGAGTTAAAATAGCAAATCAGA
AAAAGTTAAAAATAATACCC

Downstream 100 bases:

>100_bases
GCAGCCAATAGAGCAAGTCAGCCCATTTCTTGCTGAACTCCTCCCGACTGACTAAAGTTAATTCTACTAGCTTTTATGTC
GGGAGCGGTATTTATGACTG

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MTNSCKVAVGQLQVGNFVRLPIAWKDHPFIFNSFKIKQQTQIELIKNLGIEFVYVRPERSDTQPLSSIEAKQQKPQIIDN
NLEQLSGELNQQKAKSIDAQQSLRRRLQKTEKHFIRNVAMMRNLTSKLRNRPLDAVNEAKELIHGVCNQLLTDDKLVLHL
MADAKSDEGIYYHSLNVAVLSMLIAKELGWSRKEIELVGMGALFHDIGKLKIPTQLLRKTTPFTHSEQIYFNQHPAMGAE
LINITDNFPQQALPIILNHHEFLDGSGTPKGLLEKDLDKLSQLVCAINTYDNLCHSDRSRKARTPYSALGYLYKNYQSRL
NQQVINRLVKMLGVYPPGSVVELSSGQFAMVMSVNLSKLLLPRVLVYDPNVPKEQAPIVDLASEGLTIIRCLPLAALPEK
VLKYLNPREKISYFFGAEK

Sequences:

>Translated_419_residues
MTNSCKVAVGQLQVGNFVRLPIAWKDHPFIFNSFKIKQQTQIELIKNLGIEFVYVRPERSDTQPLSSIEAKQQKPQIIDN
NLEQLSGELNQQKAKSIDAQQSLRRRLQKTEKHFIRNVAMMRNLTSKLRNRPLDAVNEAKELIHGVCNQLLTDDKLVLHL
MADAKSDEGIYYHSLNVAVLSMLIAKELGWSRKEIELVGMGALFHDIGKLKIPTQLLRKTTPFTHSEQIYFNQHPAMGAE
LINITDNFPQQALPIILNHHEFLDGSGTPKGLLEKDLDKLSQLVCAINTYDNLCHSDRSRKARTPYSALGYLYKNYQSRL
NQQVINRLVKMLGVYPPGSVVELSSGQFAMVMSVNLSKLLLPRVLVYDPNVPKEQAPIVDLASEGLTIIRCLPLAALPEK
VLKYLNPREKISYFFGAEK
>Mature_418_residues
TNSCKVAVGQLQVGNFVRLPIAWKDHPFIFNSFKIKQQTQIELIKNLGIEFVYVRPERSDTQPLSSIEAKQQKPQIIDNN
LEQLSGELNQQKAKSIDAQQSLRRRLQKTEKHFIRNVAMMRNLTSKLRNRPLDAVNEAKELIHGVCNQLLTDDKLVLHLM
ADAKSDEGIYYHSLNVAVLSMLIAKELGWSRKEIELVGMGALFHDIGKLKIPTQLLRKTTPFTHSEQIYFNQHPAMGAEL
INITDNFPQQALPIILNHHEFLDGSGTPKGLLEKDLDKLSQLVCAINTYDNLCHSDRSRKARTPYSALGYLYKNYQSRLN
QQVINRLVKMLGVYPPGSVVELSSGQFAMVMSVNLSKLLLPRVLVYDPNVPKEQAPIVDLASEGLTIIRCLPLAALPEKV
LKYLNPREKISYFFGAEK

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG2206

COG function: function code T; HD-GYP domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 47493; Mature: 47362

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSCKVAVGQLQVGNFVRLPIAWKDHPFIFNSFKIKQQTQIELIKNLGIEFVYVRPERS
CCCCCEEEEEEEEECCEEEEEEEECCCCEEECCEECCHHHHHHHHHHCCCEEEEECCCCC
DTQPLSSIEAKQQKPQIIDNNLEQLSGELNQQKAKSIDAQQSLRRRLQKTEKHFIRNVAM
CCCCHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MRNLTSKLRNRPLDAVNEAKELIHGVCNQLLTDDKLVLHLMADAKSDEGIYYHSLNVAVL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEHHHHHH
SMLIAKELGWSRKEIELVGMGALFHDIGKLKIPTQLLRKTTPFTHSEQIYFNQHPAMGAE
HHHHHHHHCCCCCCEEEEECHHHHHHHCCCCCCHHHHHCCCCCCCCCCEEECCCCCCCCE
LINITDNFPQQALPIILNHHEFLDGSGTPKGLLEKDLDKLSQLVCAINTYDNLCHSDRSR
EEECCCCCCHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KARTPYSALGYLYKNYQSRLNQQVINRLVKMLGVYPPGSVVELSSGQFAMVMSVNLSKLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCHHHHH
LPRVLVYDPNVPKEQAPIVDLASEGLTIIRCLPLAALPEKVLKYLNPREKISYFFGAEK
HCCCEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
>Mature Secondary Structure 
TNSCKVAVGQLQVGNFVRLPIAWKDHPFIFNSFKIKQQTQIELIKNLGIEFVYVRPERS
CCCCEEEEEEEEECCEEEEEEEECCCCEEECCEECCHHHHHHHHHHCCCEEEEECCCCC
DTQPLSSIEAKQQKPQIIDNNLEQLSGELNQQKAKSIDAQQSLRRRLQKTEKHFIRNVAM
CCCCHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MRNLTSKLRNRPLDAVNEAKELIHGVCNQLLTDDKLVLHLMADAKSDEGIYYHSLNVAVL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEHHHHHH
SMLIAKELGWSRKEIELVGMGALFHDIGKLKIPTQLLRKTTPFTHSEQIYFNQHPAMGAE
HHHHHHHHCCCCCCEEEEECHHHHHHHCCCCCCHHHHHCCCCCCCCCCEEECCCCCCCCE
LINITDNFPQQALPIILNHHEFLDGSGTPKGLLEKDLDKLSQLVCAINTYDNLCHSDRSR
EEECCCCCCHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KARTPYSALGYLYKNYQSRLNQQVINRLVKMLGVYPPGSVVELSSGQFAMVMSVNLSKLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCHHHHH
LPRVLVYDPNVPKEQAPIVDLASEGLTIIRCLPLAALPEKVLKYLNPREKISYFFGAEK
HCCCEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]