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The map label for this gene is pleD [H]

Identifier: 157961509

GI number: 157961509

Start: 2043017

End: 2044219

Strand: Direct

Name: pleD [H]

Synonym: Spea_1684

Alternate gene names: 157961509

Gene position: 2043017-2044219 (Clockwise)

Preceding gene: 157961508

Following gene: 157961512

Centisome position: 39.48

GC content: 43.47

Gene sequence:

>1203_bases
TTGCGGATTCTAGTGGTCGAAGATAGCAAAACGGTTTCGCGGGTGATGCGCCATTTGCTTACTCAAGAGCTGAGTTGTGA
GGTGGATGTTGCGCCAGATATGGGCAGCGCAAAAGAGTTGCTGGCCCAAAATGAGTACTTTGTCGCGATTACAGATCTGA
ACTTACCAGATGCTCAAGAGGGAGAGATTGTTAAGTTTGTATTGGCAAAACAGATCCCCTGCATAGTGTTAACCGGAAGC
TGGGATGCCGAGCAACGAGAGCGTTTACTTCAGCTGGGTATCGTTGATTATGTCTTTAAAGAAAATCGTTTTAGCTATGA
ATATACTGCCAAGCTGGTTAAGCGTTTACGCCGCAATCAAAGCGTAAAGGTACTGGTTGCCGATGACTCGCTTGTGAGCC
GTAAATTTATCCGAAGTTTATTGGAGCAGCACCTTTTTCAAGTCATCGAGGCTGACGATGGTATTTCAGCGTTAGAAACC
CTTAATGATAATCCAGATATCAAACTACTTATCACAGATTACAATATGCCTGGCCTAGATGGATTCGGGCTTATTATTAA
AGTTCGTGAACAGTTTAGTCGTGAAGAGTTAGTGATTATTGGCTTATCGAGTGACAGTGATGAAAGCCTCTCTGCCCGTT
TTATCAAAAATGGCGCAAATGATTTTCTACAGAAGCCTTTTGTCCACGAAGAGTTTCACTGCCGGGTGTTGAACACGTTA
GATGCACTGGACATGGTGACACGGCTTTGGGAGCAGGCAAACCTAGATTATCTGACTAATGTTTATAATCGCCGTTATTT
CTTTAATTTATACCAAGAAAAGCTAACGGAAATTGCGCAGAAACAGGGGACACTGTCTCTGGCATTATTGGATATCGACT
TTTTCAAGAAGGTAAACGATAGCTATGGTCATGACGTGGGTGATGAAGTCTTGATTGAGTTTTCCAATCGATTACAGCAA
TCTTTCTCACAGCACTTTAACGTTGCACGTATTGGCGGCGAAGAGTTTGTGGTCGGATTTAAAGGCCTTAACGTCGAAAA
GGCTTATGCGCTATTAGATAGTTTTCGTATGCAGCTCGAAGCCAACCCCATTGAAACCTCTAAGGGCATGCTGAATATTA
GCGTGAGTAGTGGAGTGGCGGAGTTTACCCATACAGAAAATGTTGATGAGCTGCTTAATCGTGCCGACATAGGCTTACTA
TGA

Upstream 100 bases:

>100_bases
GCAAACTTAGCTCAATTAACTCCGGTTATTTGTTATCGAGAGTCTGATAAGTGGCTCGCGAAGTGAGGTGGAGAAACTAT
ATAGGAACAAGGAGAAAAGT

Downstream 100 bases:

>100_bases
GGCTAAATCGGCTGGACGTAACCTAGTCTGTATTGCTGAGTGTTAATTGGTTTCTGTGCTAGATAAAGAAGATAAAAGCA
GCCAAATGGCTGCTTTTTTC

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MRILVVEDSKTVSRVMRHLLTQELSCEVDVAPDMGSAKELLAQNEYFVAITDLNLPDAQEGEIVKFVLAKQIPCIVLTGS
WDAEQRERLLQLGIVDYVFKENRFSYEYTAKLVKRLRRNQSVKVLVADDSLVSRKFIRSLLEQHLFQVIEADDGISALET
LNDNPDIKLLITDYNMPGLDGFGLIIKVREQFSREELVIIGLSSDSDESLSARFIKNGANDFLQKPFVHEEFHCRVLNTL
DALDMVTRLWEQANLDYLTNVYNRRYFFNLYQEKLTEIAQKQGTLSLALLDIDFFKKVNDSYGHDVGDEVLIEFSNRLQQ
SFSQHFNVARIGGEEFVVGFKGLNVEKAYALLDSFRMQLEANPIETSKGMLNISVSSGVAEFTHTENVDELLNRADIGLL

Sequences:

>Translated_400_residues
MRILVVEDSKTVSRVMRHLLTQELSCEVDVAPDMGSAKELLAQNEYFVAITDLNLPDAQEGEIVKFVLAKQIPCIVLTGS
WDAEQRERLLQLGIVDYVFKENRFSYEYTAKLVKRLRRNQSVKVLVADDSLVSRKFIRSLLEQHLFQVIEADDGISALET
LNDNPDIKLLITDYNMPGLDGFGLIIKVREQFSREELVIIGLSSDSDESLSARFIKNGANDFLQKPFVHEEFHCRVLNTL
DALDMVTRLWEQANLDYLTNVYNRRYFFNLYQEKLTEIAQKQGTLSLALLDIDFFKKVNDSYGHDVGDEVLIEFSNRLQQ
SFSQHFNVARIGGEEFVVGFKGLNVEKAYALLDSFRMQLEANPIETSKGMLNISVSSGVAEFTHTENVDELLNRADIGLL
>Mature_400_residues
MRILVVEDSKTVSRVMRHLLTQELSCEVDVAPDMGSAKELLAQNEYFVAITDLNLPDAQEGEIVKFVLAKQIPCIVLTGS
WDAEQRERLLQLGIVDYVFKENRFSYEYTAKLVKRLRRNQSVKVLVADDSLVSRKFIRSLLEQHLFQVIEADDGISALET
LNDNPDIKLLITDYNMPGLDGFGLIIKVREQFSREELVIIGLSSDSDESLSARFIKNGANDFLQKPFVHEEFHCRVLNTL
DALDMVTRLWEQANLDYLTNVYNRRYFFNLYQEKLTEIAQKQGTLSLALLDIDFFKKVNDSYGHDVGDEVLIEFSNRLQQ
SFSQHFNVARIGGEEFVVGFKGLNVEKAYALLDSFRMQLEANPIETSKGMLNISVSSGVAEFTHTENVDELLNRADIGLL

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=153, Percent_Identity=33.9869281045752, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787262, Length=205, Percent_Identity=32.6829268292683, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87081881, Length=167, Percent_Identity=32.9341317365269, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787816, Length=145, Percent_Identity=35.1724137931034, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786584, Length=146, Percent_Identity=30.1369863013699, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788085, Length=150, Percent_Identity=34, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI145693134, Length=150, Percent_Identity=28, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI87082007, Length=139, Percent_Identity=30.9352517985612, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1790863, Length=121, Percent_Identity=33.0578512396694, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787056, Length=149, Percent_Identity=32.2147651006711, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1786599, Length=114, Percent_Identity=29.8245614035088, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 45596; Mature: 45596

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVVEDSKTVSRVMRHLLTQELSCEVDVAPDMGSAKELLAQNEYFVAITDLNLPDAQE
CEEEEECCCHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHCCCCEEEEEEECCCCCCCC
GEIVKFVLAKQIPCIVLTGSWDAEQRERLLQLGIVDYVFKENRFSYEYTAKLVKRLRRNQ
CCCHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
SVKVLVADDSLVSRKFIRSLLEQHLFQVIEADDGISALETLNDNPDIKLLITDYNMPGLD
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCCCCCC
GFGLIIKVREQFSREELVIIGLSSDSDESLSARFIKNGANDFLQKPFVHEEFHCRVLNTL
CCHHHEEEHHHCCCCCEEEEEECCCCCCHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHH
DALDMVTRLWEQANLDYLTNVYNRRYFFNLYQEKLTEIAQKQGTLSLALLDIDFFKKVND
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHCC
SYGHDVGDEVLIEFSNRLQQSFSQHFNVARIGGEEFVVGFKGLNVEKAYALLDSFRMQLE
CCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHC
ANPIETSKGMLNISVSSGVAEFTHTENVDELLNRADIGLL
CCCCCCCCCEEEEEECCCCHHHHCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MRILVVEDSKTVSRVMRHLLTQELSCEVDVAPDMGSAKELLAQNEYFVAITDLNLPDAQE
CEEEEECCCHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHCCCCEEEEEEECCCCCCCC
GEIVKFVLAKQIPCIVLTGSWDAEQRERLLQLGIVDYVFKENRFSYEYTAKLVKRLRRNQ
CCCHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
SVKVLVADDSLVSRKFIRSLLEQHLFQVIEADDGISALETLNDNPDIKLLITDYNMPGLD
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCCCCCC
GFGLIIKVREQFSREELVIIGLSSDSDESLSARFIKNGANDFLQKPFVHEEFHCRVLNTL
CCHHHEEEHHHCCCCCEEEEEECCCCCCHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHH
DALDMVTRLWEQANLDYLTNVYNRRYFFNLYQEKLTEIAQKQGTLSLALLDIDFFKKVND
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHCC
SYGHDVGDEVLIEFSNRLQQSFSQHFNVARIGGEEFVVGFKGLNVEKAYALLDSFRMQLE
CCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHC
ANPIETSKGMLNISVSSGVAEFTHTENVDELLNRADIGLL
CCCCCCCCCEEEEEECCCCHHHHCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA