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The map label for this gene is cysK [H]

Identifier: 157961507

GI number: 157961507

Start: 2040338

End: 2041306

Strand: Reverse

Name: cysK [H]

Synonym: Spea_1682

Alternate gene names: 157961507

Gene position: 2041306-2040338 (Counterclockwise)

Preceding gene: 157961510

Following gene: 157961505

Centisome position: 39.45

GC content: 47.16

Gene sequence:

>969_bases
ATGAGCAAAATTTTCGAAGATAATTCACAAACAATTGGTAACACCCCTCTTGTCCGCCTAAACCGCGTAAGTAATGGTAG
AGTGCTTGCAAAAGTAGAAGCGCGTAACCCAAGCTTCAGCGTTAAGTGCCGTATCGGTGCCAATATGATCTGGGATGCTG
AGAAAAAAGGCATTCTCACTCAGGAGCAAGAGCTCATTGAGCCGACATCGGGTAACACGGGTATCGCACTCGCTTATGTT
GCCGCTGCTCGCGGATATAAGTTAACCCTCACTATGCCAAACACCATGAGTTTAGAGCGTAGAAAGTTACTCAAAGCCCT
TGGTGCAAATCTTGTACTCACCGATGGCGCTAAAGGTATGAAAGGCGCGATTGATAAGGCTGAAGAGATCAGACAGACTG
CCCCAGAAAAGTACGTCTTACTGCAGCAGTTCAACAACCCTGCTAACCCAGAAATTCATGAGAAAACGACCGGCCCAGAG
ATCTGGAATGATACCGACGGTGCAGTCGATGTTGTTGTTGCCGGTGTAGGTACAGGTGGCACCATTACCGGTGTTAGCCG
TTACATTAAGAACACTCAAGGCAAGGCGATCACTTCGGTTGCGGTTGAGCCAACAGACTCACCAGTTATTGCGCAGACGC
TTGCGGGCCAAACTGTACAACCTGGACCACACAAAATCCAAGGTATTGGCGCTGGTTTTATTCCTGGCAATTTAGATCTT
AATCTTGTCGATCGTGTTGAAGCCGTAACCAATGAAGAGTCTATCGAGATGGCTCAGCGCTTAATGAAAGAAGAAGGGTT
CCTTGTGGGTATTTCTTCAGGCGCCGCCGTTGTGGCAGCTAACCGTATCGCTGCATTGCCTGAATTTGAAGGGCAAAACA
TTGTGGTTATTTTACCTTCTGCTGCAGAACGCTACTTATCTTCAGTACTGTTTCAAGGTGAATTTAGCGACGCTGAAAAC
GTTCAATAA

Upstream 100 bases:

>100_bases
CCTTTAATAACTAAATAGAATAACGAAGAACAAACTATCACTTCTATTGCTGCCAAAGCCTATTATGATTTAGCTGTGCA
TTTAAAGGAGCAAGCAACCC

Downstream 100 bases:

>100_bases
TTTGCGCCATGTTTAATAAAAAACCCAAGCCTTAGCTTGGGTTTTTTATTGCCATCACAATATGCGAGACCGTTGAGCTG
ACTATCTCTAGCAAAGCATG

Product: cysteine synthase A

Products: NA

Alternate protein names: CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; Sulfate starvation-induced protein 5; SSI5 [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSKIFEDNSQTIGNTPLVRLNRVSNGRVLAKVEARNPSFSVKCRIGANMIWDAEKKGILTQEQELIEPTSGNTGIALAYV
AAARGYKLTLTMPNTMSLERRKLLKALGANLVLTDGAKGMKGAIDKAEEIRQTAPEKYVLLQQFNNPANPEIHEKTTGPE
IWNDTDGAVDVVVAGVGTGGTITGVSRYIKNTQGKAITSVAVEPTDSPVIAQTLAGQTVQPGPHKIQGIGAGFIPGNLDL
NLVDRVEAVTNEESIEMAQRLMKEEGFLVGISSGAAVVAANRIAALPEFEGQNIVVILPSAAERYLSSVLFQGEFSDAEN
VQ

Sequences:

>Translated_322_residues
MSKIFEDNSQTIGNTPLVRLNRVSNGRVLAKVEARNPSFSVKCRIGANMIWDAEKKGILTQEQELIEPTSGNTGIALAYV
AAARGYKLTLTMPNTMSLERRKLLKALGANLVLTDGAKGMKGAIDKAEEIRQTAPEKYVLLQQFNNPANPEIHEKTTGPE
IWNDTDGAVDVVVAGVGTGGTITGVSRYIKNTQGKAITSVAVEPTDSPVIAQTLAGQTVQPGPHKIQGIGAGFIPGNLDL
NLVDRVEAVTNEESIEMAQRLMKEEGFLVGISSGAAVVAANRIAALPEFEGQNIVVILPSAAERYLSSVLFQGEFSDAEN
VQ
>Mature_321_residues
SKIFEDNSQTIGNTPLVRLNRVSNGRVLAKVEARNPSFSVKCRIGANMIWDAEKKGILTQEQELIEPTSGNTGIALAYVA
AARGYKLTLTMPNTMSLERRKLLKALGANLVLTDGAKGMKGAIDKAEEIRQTAPEKYVLLQQFNNPANPEIHEKTTGPEI
WNDTDGAVDVVVAGVGTGGTITGVSRYIKNTQGKAITSVAVEPTDSPVIAQTLAGQTVQPGPHKIQGIGAGFIPGNLDLN
LVDRVEAVTNEESIEMAQRLMKEEGFLVGISSGAAVVAANRIAALPEFEGQNIVVILPSAAERYLSSVLFQGEFSDAENV
Q

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=319, Percent_Identity=39.4984326018809, Blast_Score=194, Evalue=7e-50,
Organism=Homo sapiens, GI295821200, Length=319, Percent_Identity=39.4984326018809, Blast_Score=194, Evalue=7e-50,
Organism=Homo sapiens, GI4557415, Length=319, Percent_Identity=39.4984326018809, Blast_Score=194, Evalue=7e-50,
Organism=Escherichia coli, GI1788754, Length=320, Percent_Identity=76.875, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI2367138, Length=306, Percent_Identity=43.1372549019608, Blast_Score=207, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17535051, Length=323, Percent_Identity=49.2260061919505, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI115535073, Length=314, Percent_Identity=53.1847133757962, Blast_Score=277, Evalue=6e-75,
Organism=Caenorhabditis elegans, GI17562970, Length=312, Percent_Identity=48.0769230769231, Blast_Score=274, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI32566674, Length=307, Percent_Identity=48.8599348534202, Blast_Score=271, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17561720, Length=322, Percent_Identity=46.8944099378882, Blast_Score=245, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI32566672, Length=220, Percent_Identity=47.2727272727273, Blast_Score=197, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI17534315, Length=317, Percent_Identity=41.0094637223975, Blast_Score=158, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI25147552, Length=317, Percent_Identity=36.9085173501577, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17561716, Length=135, Percent_Identity=42.962962962963, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321594, Length=315, Percent_Identity=37.4603174603175, Blast_Score=172, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6321449, Length=324, Percent_Identity=33.3333333333333, Blast_Score=126, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24643623, Length=309, Percent_Identity=37.5404530744337, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI20129101, Length=309, Percent_Identity=37.5404530744337, Blast_Score=159, Evalue=4e-39,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34354; Mature: 34222

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIFEDNSQTIGNTPLVRLNRVSNGRVLAKVEARNPSFSVKCRIGANMIWDAEKKGILT
CCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCEEEEEEECCCEEECCCCCCCCC
QEQELIEPTSGNTGIALAYVAAARGYKLTLTMPNTMSLERRKLLKALGANLVLTDGAKGM
CHHHHCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCC
KGAIDKAEEIRQTAPEKYVLLQQFNNPANPEIHEKTTGPEIWNDTDGAVDVVVAGVGTGG
HHHHHHHHHHHHHCCHHEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
TITGVSRYIKNTQGKAITSVAVEPTDSPVIAQTLAGQTVQPGPHKIQGIGAGFIPGNLDL
HHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCC
NLVDRVEAVTNEESIEMAQRLMKEEGFLVGISSGAAVVAANRIAALPEFEGQNIVVILPS
HHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCEEEEECHHEECCCCCCCEEEEEECC
AAERYLSSVLFQGEFSDAENVQ
HHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SKIFEDNSQTIGNTPLVRLNRVSNGRVLAKVEARNPSFSVKCRIGANMIWDAEKKGILT
CCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCEEEEEEECCCEEECCCCCCCCC
QEQELIEPTSGNTGIALAYVAAARGYKLTLTMPNTMSLERRKLLKALGANLVLTDGAKGM
CHHHHCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCC
KGAIDKAEEIRQTAPEKYVLLQQFNNPANPEIHEKTTGPEIWNDTDGAVDVVVAGVGTGG
HHHHHHHHHHHHHCCHHEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
TITGVSRYIKNTQGKAITSVAVEPTDSPVIAQTLAGQTVQPGPHKIQGIGAGFIPGNLDL
HHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCC
NLVDRVEAVTNEESIEMAQRLMKEEGFLVGISSGAAVVAANRIAALPEFEGQNIVVILPS
HHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCEEEEECHHEECCCCCCCEEEEEECC
AAERYLSSVLFQGEFSDAENVQ
HHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]