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The map label for this gene is focA [H]

Identifier: 157961499

GI number: 157961499

Start: 2030945

End: 2032426

Strand: Direct

Name: focA [H]

Synonym: Spea_1674

Alternate gene names: 157961499

Gene position: 2030945-2032426 (Clockwise)

Preceding gene: 157961495

Following gene: 157961501

Centisome position: 39.25

GC content: 45.55

Gene sequence:

>1482_bases
ATGGCGTCTAATCAAGTGGTTAAAGAACAGGTGCTAGTAAACCCTTTACAGCAAAGAGCTGAGAGCCCCAGAGCCGAACT
ACTAAGTCTATACCAACATGCCGAGTATTATGGCAAGGCAAAGGTGATTAAATCGGCATGGCAGTCCTTTGGTTTAGCTG
CATTTGCTGGTGCTTTTATTGCCTTGGCGTTTGTTTTTTATATCACAGTGACAACGGGAAGTGATGGGTCGTGGGGCTTA
GTGCGTCTAGCGGGGGGACTCGCATTTAGTTTAGGTCTGATGTTAGTGGTTATCTGTGGCGGCGAGCTTTTTACCAGTAC
CGTTCTGAGTAGCGTTGCCTGGGCTCAAAAGCTTGTTACAACACGAGACCTTATTAAATGTTGGGCTCGAGTATACCTAG
GTAACCTCATCGGCGCCGTGATCATGTTGAGTTTGATTATGTCTGCTCGAATGTACGAACTCGACGGGGGCCAGTGGGGG
ATCAATGCACTAAAAATAGCACAGCATAAGCTGCACCATAGCTGGGGGCAGGCCTTCGCTCTCGGTGTGTTGTGCAATAT
GTTGGTTTGCTTGGGCGTGTGGATGACTTTTTCGAGTCGTGACGCACTTACCAAGGCGATCTTACTTATGCTTCCCGTAG
CTATGTTTGTCAGTAGTGGTTTCGAGCACAGCATTGCGAACCTGTTTATGGTGCCTCTTGGGATCACCATCCATCAGTTT
GCCGATCCCGCCTGGTTTGAAGCGCTAAACATTAGTCGCGATACGTTTGCGGATCTGACTCTTAGCAATTTTATTTTTAG
TAATCTTATTCCTGTGACCTTAGGAAATATCGTCGGTGGTGGCGTTTTTGTTGGCTTAGGCTACTGGCTTATCGAAAAGT
CAGATCAAAGCGCGAATGCTTCTTTTACTCATCGTGTACCAAAACTTGAAACATCGGCTCAAATACATCAAATACCAACT
TCTGGAGTACTCATCAAAATGCCTAAAACAATAAAAAAACTATCAGTAAAAGATCTTATGGATCCAACGCCACTGACTCT
CTCTTCTGAGCTGTCTGTCTATGCTGCGTTGAGCTTACTTGCAGAAAATGAGTGTCGTAGTGCACCAGTCCTCGACGAAA
AAAAGCGTTTGCTTGGTTTTATCTCACAGCAAGATCTACTTCGTAGCCTTTGGTCTGAAGAGTTTGCCAGAGGTGTTTCA
TGTAAGGTTAGTGATCTGATGCAAACGGAGGTGATGACGTTATCGCCACAGGACTCAGTTGCTGAGCTGATAGAGTTAAT
GGTTGTCGATAGAAGTAAGTTATTCCCGGTGAACGAGCAGGGGATCTTAACAGGGAATACTTTTAAAAGTTATGAGGAGC
GACTACGCAGTGCGTCGCCAAATAAGCCTAGTGTTTTTCCTGTTTTAGATAATGGCCTACTGTGTGGGGTGATCACGCGT
GAGGCGATAGCCAAAAAAGTGTGCGACATTTATAGAGTCTAA

Upstream 100 bases:

>100_bases
TAAATATACTTGTGAGCACCAAATAACGGTTAGTTGATATAAAGGAGGAATAGAGTAATGAAAATAACAGACTTCTACGT
TTCTAGTTGGAGTGGAATAA

Downstream 100 bases:

>100_bases
ATCTTTAGGCTAAAAAGATAAAACGGGACGATGATTATCGTCCCGTTTTTTTTACTTCTTTATCTGCCGATTACGGCACA
ATCTTTTTGCCTCTGTGATA

Product: formate/nitrite transporter

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: Formate channel 1 [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MASNQVVKEQVLVNPLQQRAESPRAELLSLYQHAEYYGKAKVIKSAWQSFGLAAFAGAFIALAFVFYITVTTGSDGSWGL
VRLAGGLAFSLGLMLVVICGGELFTSTVLSSVAWAQKLVTTRDLIKCWARVYLGNLIGAVIMLSLIMSARMYELDGGQWG
INALKIAQHKLHHSWGQAFALGVLCNMLVCLGVWMTFSSRDALTKAILLMLPVAMFVSSGFEHSIANLFMVPLGITIHQF
ADPAWFEALNISRDTFADLTLSNFIFSNLIPVTLGNIVGGGVFVGLGYWLIEKSDQSANASFTHRVPKLETSAQIHQIPT
SGVLIKMPKTIKKLSVKDLMDPTPLTLSSELSVYAALSLLAENECRSAPVLDEKKRLLGFISQQDLLRSLWSEEFARGVS
CKVSDLMQTEVMTLSPQDSVAELIELMVVDRSKLFPVNEQGILTGNTFKSYEERLRSASPNKPSVFPVLDNGLLCGVITR
EAIAKKVCDIYRV

Sequences:

>Translated_493_residues
MASNQVVKEQVLVNPLQQRAESPRAELLSLYQHAEYYGKAKVIKSAWQSFGLAAFAGAFIALAFVFYITVTTGSDGSWGL
VRLAGGLAFSLGLMLVVICGGELFTSTVLSSVAWAQKLVTTRDLIKCWARVYLGNLIGAVIMLSLIMSARMYELDGGQWG
INALKIAQHKLHHSWGQAFALGVLCNMLVCLGVWMTFSSRDALTKAILLMLPVAMFVSSGFEHSIANLFMVPLGITIHQF
ADPAWFEALNISRDTFADLTLSNFIFSNLIPVTLGNIVGGGVFVGLGYWLIEKSDQSANASFTHRVPKLETSAQIHQIPT
SGVLIKMPKTIKKLSVKDLMDPTPLTLSSELSVYAALSLLAENECRSAPVLDEKKRLLGFISQQDLLRSLWSEEFARGVS
CKVSDLMQTEVMTLSPQDSVAELIELMVVDRSKLFPVNEQGILTGNTFKSYEERLRSASPNKPSVFPVLDNGLLCGVITR
EAIAKKVCDIYRV
>Mature_492_residues
ASNQVVKEQVLVNPLQQRAESPRAELLSLYQHAEYYGKAKVIKSAWQSFGLAAFAGAFIALAFVFYITVTTGSDGSWGLV
RLAGGLAFSLGLMLVVICGGELFTSTVLSSVAWAQKLVTTRDLIKCWARVYLGNLIGAVIMLSLIMSARMYELDGGQWGI
NALKIAQHKLHHSWGQAFALGVLCNMLVCLGVWMTFSSRDALTKAILLMLPVAMFVSSGFEHSIANLFMVPLGITIHQFA
DPAWFEALNISRDTFADLTLSNFIFSNLIPVTLGNIVGGGVFVGLGYWLIEKSDQSANASFTHRVPKLETSAQIHQIPTS
GVLIKMPKTIKKLSVKDLMDPTPLTLSSELSVYAALSLLAENECRSAPVLDEKKRLLGFISQQDLLRSLWSEEFARGVSC
KVSDLMQTEVMTLSPQDSVAELIELMVVDRSKLFPVNEQGILTGNTFKSYEERLRSASPNKPSVFPVLDNGLLCGVITRE
AIAKKVCDIYRV

Specific function: Involved in the bidirectional transport of formate [H]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI1787132, Length=277, Percent_Identity=49.8194945848375, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1788837, Length=255, Percent_Identity=38.8235294117647, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI87082249, Length=272, Percent_Identity=32.3529411764706, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321779, Length=212, Percent_Identity=31.1320754716981, Blast_Score=100, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 54028; Mature: 53897

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: PS01005 FORMATE_NITRITE_TP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCC
>Mature Secondary Structure 
ASNQVVKEQVLVNPLQQRAESPRAELLSLYQHAEYYGKAKVIKSAWQSFGLAAFAGAFI
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH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HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]