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The map label for this gene is rlmI [H]

Identifier: 157961491

GI number: 157961491

Start: 2018179

End: 2019090

Strand: Direct

Name: rlmI [H]

Synonym: Spea_1666

Alternate gene names: 157961491

Gene position: 2018179-2019090 (Clockwise)

Preceding gene: 157961490

Following gene: 157961495

Centisome position: 39.0

GC content: 43.53

Gene sequence:

>912_bases
ATGCAGTTATTTATTGAAGCAGTAAAGCAGGTTGATTTTCCTGAAGACGTGACTCGTTTATTTCATGGGCGAGGAGGGCG
TTTTTCTGGCTGTGAACACTTGTGTTTGGATTGGTATTCACCTGTGGTGCTATTGACCAGTTTTAAGGTATTAGAAAGTG
CAGAGCGTGATGCGTTATGTGCGGCTATTGAGCAGCGTTGGTTTGAGCTTAGAGGCGATGAGCCGCTCAATCTGGTTTTT
CAATATCGTCGTGGCGGTGAAACTCACACCGAAATACTTAAAGGTGAAGTCCCAGTAAAGCATATCGTAACTGAAAATGG
TGCTTGTTTTCAGGTGCATTTGTTACGCGGTCAAAACCACGGCTTATTTCTAGATATGGCAAACGGTCGTCAATGGGTAC
GTGAACACAGTGCCCAGAAGAAAGTACTGAACCTATTTGCCTATACTTGTGGTTTTTCAGTTGCAGCACTGCAAGGTAAA
GCTGATGAAGTTGTGAATATCGACATGAGTAAAGGGGCGCTGTCTATTGGAAAGCAAAACCATATACTCAACGATTTCAA
AGCTGGTGCACGCTTTCTTGGTCATGACATATTTAAATCATGGGGTAAGCTGACAAAGTTAGGGCCTTATGATTTGATTA
TTGCCGATCCACCAAGCAATCAAAAAGGCAGCTTTGTTGCGACTAAAGATTATGCTCGTTTGCTCAAGAGACTTCCTGAG
TTGTTAAGTGATGACGGCGATATGCTGCTTTGCTTAAATGCGCCAGAGTTGAGTATCGAGTTCTTAATGGATCAGGTAAA
AGAGTACAGCCCAAGCCTAGTGTTTTGTGAGCAGCTTGAAAATCCAGCTGTGTTTGCAGACGTGGATCCTAACAAAGCCC
TTAAAGTGCTGCGATATCGAAAGCAGTGCTAG

Upstream 100 bases:

>100_bases
AGTGGAGAGTAAGCAGCTAGAAAACTCTCAATTAGAGCAATCTCGAGTAGAGAGCGAGCAGCAAGACAGTAATTCGTAAA
AATAGTGAAAAGTGAGTTTG

Downstream 100 bases:

>100_bases
CTAGTTAAAGTGAGTTTCTTGAAGGCCAAACATGATGTTTGGCCTTTTTTATGGCTTATCTTGTTGGTATATTTTTTCGA
CGTTAAAAAGCCCCTGTTAA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MQLFIEAVKQVDFPEDVTRLFHGRGGRFSGCEHLCLDWYSPVVLLTSFKVLESAERDALCAAIEQRWFELRGDEPLNLVF
QYRRGGETHTEILKGEVPVKHIVTENGACFQVHLLRGQNHGLFLDMANGRQWVREHSAQKKVLNLFAYTCGFSVAALQGK
ADEVVNIDMSKGALSIGKQNHILNDFKAGARFLGHDIFKSWGKLTKLGPYDLIIADPPSNQKGSFVATKDYARLLKRLPE
LLSDDGDMLLCLNAPELSIEFLMDQVKEYSPSLVFCEQLENPAVFADVDPNKALKVLRYRKQC

Sequences:

>Translated_303_residues
MQLFIEAVKQVDFPEDVTRLFHGRGGRFSGCEHLCLDWYSPVVLLTSFKVLESAERDALCAAIEQRWFELRGDEPLNLVF
QYRRGGETHTEILKGEVPVKHIVTENGACFQVHLLRGQNHGLFLDMANGRQWVREHSAQKKVLNLFAYTCGFSVAALQGK
ADEVVNIDMSKGALSIGKQNHILNDFKAGARFLGHDIFKSWGKLTKLGPYDLIIADPPSNQKGSFVATKDYARLLKRLPE
LLSDDGDMLLCLNAPELSIEFLMDQVKEYSPSLVFCEQLENPAVFADVDPNKALKVLRYRKQC
>Mature_303_residues
MQLFIEAVKQVDFPEDVTRLFHGRGGRFSGCEHLCLDWYSPVVLLTSFKVLESAERDALCAAIEQRWFELRGDEPLNLVF
QYRRGGETHTEILKGEVPVKHIVTENGACFQVHLLRGQNHGLFLDMANGRQWVREHSAQKKVLNLFAYTCGFSVAALQGK
ADEVVNIDMSKGALSIGKQNHILNDFKAGARFLGHDIFKSWGKLTKLGPYDLIIADPPSNQKGSFVATKDYARLLKRLPE
LLSDDGDMLLCLNAPELSIEFLMDQVKEYSPSLVFCEQLENPAVFADVDPNKALKVLRYRKQC

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=269, Percent_Identity=27.1375464684015, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 34203; Mature: 34203

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLFIEAVKQVDFPEDVTRLFHGRGGRFSGCEHLCLDWYSPVVLLTSFKVLESAERDALC
CHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
AAIEQRWFELRGDEPLNLVFQYRRGGETHTEILKGEVPVKHIVTENGACFQVHLLRGQNH
HHHHHHHHHCCCCCCHHHHEEECCCCCHHHHHHCCCCCHHHEEECCCCEEEEEEEECCCC
GLFLDMANGRQWVREHSAQKKVLNLFAYTCGFSVAALQGKADEVVNIDMSKGALSIGKQN
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEECCCCCHHCCCCC
HILNDFKAGARFLGHDIFKSWGKLTKLGPYDLIIADPPSNQKGSFVATKDYARLLKRLPE
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHH
LLSDDGDMLLCLNAPELSIEFLMDQVKEYSPSLVFCEQLENPAVFADVDPNKALKVLRYR
HHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEHHHCCCCEEEEECCHHHHHHHHHHH
KQC
HCC
>Mature Secondary Structure
MQLFIEAVKQVDFPEDVTRLFHGRGGRFSGCEHLCLDWYSPVVLLTSFKVLESAERDALC
CHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
AAIEQRWFELRGDEPLNLVFQYRRGGETHTEILKGEVPVKHIVTENGACFQVHLLRGQNH
HHHHHHHHHCCCCCCHHHHEEECCCCCHHHHHHCCCCCHHHEEECCCCEEEEEEEECCCC
GLFLDMANGRQWVREHSAQKKVLNLFAYTCGFSVAALQGKADEVVNIDMSKGALSIGKQN
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEECCCCCHHCCCCC
HILNDFKAGARFLGHDIFKSWGKLTKLGPYDLIIADPPSNQKGSFVATKDYARLLKRLPE
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHH
LLSDDGDMLLCLNAPELSIEFLMDQVKEYSPSLVFCEQLENPAVFADVDPNKALKVLRYR
HHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEHHHCCCCEEEEECCHHHHHHHHHHH
KQC
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA