Click here to switch to the map view.

The map label for this gene is xthA [H]

Identifier: 157961464

GI number: 157961464

Start: 1984185

End: 1984991

Strand: Reverse

Name: xthA [H]

Synonym: Spea_1638

Alternate gene names: 157961464

Gene position: 1984991-1984185 (Counterclockwise)

Preceding gene: 157961465

Following gene: 157961460

Centisome position: 38.36

GC content: 47.71

Gene sequence:

>807_bases
GTGAAGATTGTTTCATTTAATATCAATGGATTGCGAGCAAGACTGCACCAACTTCAAGCGCTGATTGATAGCCATCAACC
AGACATTATCGGCCTGCAAGAGACTAAGGTGCATGACGAAGCGTTTCCTTTCGCTGACGTTGAAGCCATGGGCTACAAGG
TGCATTACCATGGCGGTAAGGCCCATTATGGCGTTGCCATGCTGTCTAAGCTTGAGCCTATAAAGGTACAGAAAGGCTTT
CCGACCGATGAAGAGGATGCACAGCGCCGGATGATCATGGGCACCTTTACCCAAGAAAACGGCCGTCCATTAACCGTACT
CAATGGTTACTTCCCTCAAGGCGATAACATCAGCCATGAAACCAAGTACCCGGCTAAGCGTAAGTTCTACAAAGACTTAA
TGCTTTATCTGAATGAATATCACAGCAGTGACGAAGATATCGCTATTATTGGCGACATCAACATCTCTCCTGTGGACTTA
GATATCGGTATTGGCGAGCCTAACGCTAAACGCTGGCTTAAAACTGGCAAGTGTAGCTTCCAACCGGAAGAGCGTGAGTG
GCTAAAAACCTTGATGGATTGGGGTTTAGTCGATACCTTCCGTGAACTCCACCCAGAGCGTAGTGAACGTTACTCTTGGT
TTGACTATCGCAGTAAAGGCTTTGTCGATAATCGCGGCCTACGCATCGACGTTGTCTTGGCTACCAAGACATTGGCTGAG
CGCCTCACTGAGTCGGATGTTGATTACGATCTACGCGGCATCGACAAGCCATCTGATCATGCGCCGATTTGGAGCACTTT
TAGTTAA

Upstream 100 bases:

>100_bases
CTGGGCCGATGCCGACCCTTATATCGCTGCAGGCGTATATGAAAGCGTTATCGTTAAGCCTTACAAACTTGTGCTACCGT
AAATTTAAAAGGACCATAAA

Downstream 100 bases:

>100_bases
TTAAGCTTTAAGCTGATTGCTATAAGCTAGCTCATTCAAATCTGATCAACATAAAAAATGCCAGCAATAGCTGGCATTTT
TTTAACATATTCAAAAGCTT

Product: exonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKIVSFNINGLRARLHQLQALIDSHQPDIIGLQETKVHDEAFPFADVEAMGYKVHYHGGKAHYGVAMLSKLEPIKVQKGF
PTDEEDAQRRMIMGTFTQENGRPLTVLNGYFPQGDNISHETKYPAKRKFYKDLMLYLNEYHSSDEDIAIIGDINISPVDL
DIGIGEPNAKRWLKTGKCSFQPEEREWLKTLMDWGLVDTFRELHPERSERYSWFDYRSKGFVDNRGLRIDVVLATKTLAE
RLTESDVDYDLRGIDKPSDHAPIWSTFS

Sequences:

>Translated_268_residues
MKIVSFNINGLRARLHQLQALIDSHQPDIIGLQETKVHDEAFPFADVEAMGYKVHYHGGKAHYGVAMLSKLEPIKVQKGF
PTDEEDAQRRMIMGTFTQENGRPLTVLNGYFPQGDNISHETKYPAKRKFYKDLMLYLNEYHSSDEDIAIIGDINISPVDL
DIGIGEPNAKRWLKTGKCSFQPEEREWLKTLMDWGLVDTFRELHPERSERYSWFDYRSKGFVDNRGLRIDVVLATKTLAE
RLTESDVDYDLRGIDKPSDHAPIWSTFS
>Mature_268_residues
MKIVSFNINGLRARLHQLQALIDSHQPDIIGLQETKVHDEAFPFADVEAMGYKVHYHGGKAHYGVAMLSKLEPIKVQKGF
PTDEEDAQRRMIMGTFTQENGRPLTVLNGYFPQGDNISHETKYPAKRKFYKDLMLYLNEYHSSDEDIAIIGDINISPVDL
DIGIGEPNAKRWLKTGKCSFQPEEREWLKTLMDWGLVDTFRELHPERSERYSWFDYRSKGFVDNRGLRIDVVLATKTLAE
RLTESDVDYDLRGIDKPSDHAPIWSTFS

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=275, Percent_Identity=28, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI18375503, Length=275, Percent_Identity=28, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI18375501, Length=275, Percent_Identity=28, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=61.4232209737828, Blast_Score=360, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71989536, Length=271, Percent_Identity=26.9372693726937, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI221330655, Length=279, Percent_Identity=31.8996415770609, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17136678, Length=279, Percent_Identity=31.8996415770609, Blast_Score=106, Evalue=1e-23,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30880; Mature: 30880

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVSFNINGLRARLHQLQALIDSHQPDIIGLQETKVHDEAFPFADVEAMGYKVHYHGGK
CEEEEECCHHHHHHHHHHHHHHHCCCCCEECCHHCCCCCCCCCCCCHHHEEEEEEECCCC
AHYGVAMLSKLEPIKVQKGFPTDEEDAQRRMIMGTFTQENGRPLTVLNGYFPQGDNISHE
HHHHHHHHHCCCCEEEECCCCCCHHHHHHEEEEEEEECCCCCEEEEEECCCCCCCCCCCC
TKYPAKRKFYKDLMLYLNEYHSSDEDIAIIGDINISPVDLDIGIGEPNAKRWLKTGKCSF
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEECCCCCHHHHHHCCCCCC
QPEEREWLKTLMDWGLVDTFRELHPERSERYSWFDYRSKGFVDNRGLRIDVVLATKTLAE
CCHHHHHHHHHHHCCHHHHHHHHCCCHHCCCCEECCCCCCCCCCCCCEEEEEEHHHHHHH
RLTESDVDYDLRGIDKPSDHAPIWSTFS
HHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIVSFNINGLRARLHQLQALIDSHQPDIIGLQETKVHDEAFPFADVEAMGYKVHYHGGK
CEEEEECCHHHHHHHHHHHHHHHCCCCCEECCHHCCCCCCCCCCCCHHHEEEEEEECCCC
AHYGVAMLSKLEPIKVQKGFPTDEEDAQRRMIMGTFTQENGRPLTVLNGYFPQGDNISHE
HHHHHHHHHCCCCEEEECCCCCCHHHHHHEEEEEEEECCCCCEEEEEECCCCCCCCCCCC
TKYPAKRKFYKDLMLYLNEYHSSDEDIAIIGDINISPVDLDIGIGEPNAKRWLKTGKCSF
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEECCCCCHHHHHHCCCCCC
QPEEREWLKTLMDWGLVDTFRELHPERSERYSWFDYRSKGFVDNRGLRIDVVLATKTLAE
CCHHHHHHHHHHHCCHHHHHHHHCCCHHCCCCEECCCCCCCCCCCCCEEEEEEHHHHHHH
RLTESDVDYDLRGIDKPSDHAPIWSTFS
HHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]