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The map label for this gene is mnmC

Identifier: 157961431

GI number: 157961431

Start: 1943701

End: 1945626

Strand: Reverse

Name: mnmC

Synonym: Spea_1605

Alternate gene names: 157961431

Gene position: 1945626-1943701 (Counterclockwise)

Preceding gene: 157961434

Following gene: 157961426

Centisome position: 37.6

GC content: 47.56

Gene sequence:

>1926_bases
ATGACAGTAAGTAAAATTCTCAAGCAGATCATCGACAGTCAAACCACTAAAAATGTGGTCATTGGCCAGTTAGGTTTAGG
TAGCATTACCAAACTTAAACAGCTGATAACTCTACTCGCTCACCATAAAGACAAGCAGCTTACGCTGAAGTTGTTTGTCG
ATATAAGCGATACCTCAGGTATCTTACTTGCTAATGAAGAGTTAACGACACTGCTATGTCCTCAGGGGAGCATTTCTTTA
ACCGCAATAGAAGGCTGTCAGCGAGTCATCGTTAGTAATTGCAAACTCACTATCGATTTTTATCTCGGCCAATACCTCAC
CCAACTGCAGGCTTTGCCTATGGTTAACGATGGTTTTGTCGACGGCTGGCACGTTACAAGTGATGCAGACCAAACTCAGT
TAAGGCAAATACTGTTTTGGCAGCTAGCAAAATTAAGTAAAAATGATGCCCAGTTCAGCCTTGATGATACATTTACCGAA
GTTTCTCAGCTAGAACTCTATACTCAGGCCGAGCAAGTGGGGCTTTATCGCTATGCTGACAACATTCCAGGAAATATACA
GAGCGGCGATGAGATCTGTTTTCAAGAACGAGCCGCGATGCGTGCGCAGAGCCGAGCGTTGCAAGCCCCCTACCCAATCT
GTTCTGCTGCTGTTACAACCCATGCAACATGCAGCAACCTAGGCATCGCCATTATTGGCGGCGGCGTCGCCAGCGCGTGT
TTGGCTTTATCCTTAGCCGAGCGAGGACAGCAAGTCACACTTTTTTGTGAAGACCATGCCCTAGCGCAAGCCGCCTCTGG
CAACAAGCAAGGGGCGATCTATCCGTTACTCACGCCAGATAACAACACCTTAAGCCAGTATTTTCAGCAGGCCTACCTGT
TTAGCTTACAAAGACTCAAGAGCCTAGCAGCGCAAGGACACCCAATTGATTTTGACCTGTGCGGCGTAGTGCACACCGGG
CATGATGAACGCAGCCGCAAGCGAGTGGCTAAGATTATTAATGGTCAAAACTGGCAGCCGAGCATCGCCCGGGCAATTAC
AGCCGAGCAGGCCTCTAGCATTGCTGGATTGAAGATTGATGATGGAGGTATTTTCTACCCTATGGGTGGCTGGGTGTCTC
CACAAGACTTTACCCGAGCGGCATTTAATCAGGCTAAGGCGATTGCTGGTGCATCACTTAAACTCAATACACAAATAACC
GATATTCACTACAAGGATGGTGGCTGGGAGCTAACTAGTAACACCGAACGCTTTGGCTCCTTTAAGGCGCTTATCCTTGC
TAACGGTAAGTCGATAACTCAATTCCCGCAAACCCAATACCTACAGGCTACGGGATTTAGAGGTCAAGTCAGTCATGTGC
CTTCTCGAGCTAAACTTTCAAAACTTAGCAGTGTTTTGTGCGCCCATGGCTATATGACACCGAGCAACAATACATTACAT
TGCCTAGGCGCAAGCTACGTTAAAAATGCGCCAAATACTGATTATTGCCCGAATGAGCAGGTCGAAAACTTACATAAAAT
TCAACATAGTTATGTTGGCCAAGAGTGGGTGGAAGACATTGATGTATCTGGCCATAGCGCTCGTGTTGGCGTACGTATGG
TAACCCGCGATCATGCTCCTATGATGGGTCCCGCCCCCGATATCGACTCAATTATGACTCTATATCAAGATCATCAGTTA
ACACCGCAGAGCCGTAAATACTGGCAAAGCCATAATGCGCCAGTTCATCAAGGTCTGTATGTGCTGGGAGGATTAGGCTC
GCGAGGCTTAAGCTCTGGGCCACTCGCCGCAGAATCGCTCGCAGCGCAGATTTGTGGCGATTTAATGCCTATTAGTCGTG
ATTTTGTGGCACTACTCAACCCTAATAGAATGTGGATGCGAAAACTACTCAAAGGCAAAGCGCTAGAGGTGGGTGTCGAG
GTTTAG

Upstream 100 bases:

>100_bases
TCTTTGCCAAAATATGCCCATAAAAATGTACAATTTCCGCAAGTAAAACCGATTATAAAGCATATTTGTGAGTGCTTAAT
TTAACATTAAAGACGTAACC

Downstream 100 bases:

>100_bases
ATCACAGCTTGTCTGCCTTCACACTCAAGTCTATAAACCCGAGTTATAAACCCCAAAAGCGCCTAATTGGCGCTTTGTTT
TTCCCTTACGAGTTCTATTC

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 641; Mature: 640

Protein sequence:

>641_residues
MTVSKILKQIIDSQTTKNVVIGQLGLGSITKLKQLITLLAHHKDKQLTLKLFVDISDTSGILLANEELTTLLCPQGSISL
TAIEGCQRVIVSNCKLTIDFYLGQYLTQLQALPMVNDGFVDGWHVTSDADQTQLRQILFWQLAKLSKNDAQFSLDDTFTE
VSQLELYTQAEQVGLYRYADNIPGNIQSGDEICFQERAAMRAQSRALQAPYPICSAAVTTHATCSNLGIAIIGGGVASAC
LALSLAERGQQVTLFCEDHALAQAASGNKQGAIYPLLTPDNNTLSQYFQQAYLFSLQRLKSLAAQGHPIDFDLCGVVHTG
HDERSRKRVAKIINGQNWQPSIARAITAEQASSIAGLKIDDGGIFYPMGGWVSPQDFTRAAFNQAKAIAGASLKLNTQIT
DIHYKDGGWELTSNTERFGSFKALILANGKSITQFPQTQYLQATGFRGQVSHVPSRAKLSKLSSVLCAHGYMTPSNNTLH
CLGASYVKNAPNTDYCPNEQVENLHKIQHSYVGQEWVEDIDVSGHSARVGVRMVTRDHAPMMGPAPDIDSIMTLYQDHQL
TPQSRKYWQSHNAPVHQGLYVLGGLGSRGLSSGPLAAESLAAQICGDLMPISRDFVALLNPNRMWMRKLLKGKALEVGVE
V

Sequences:

>Translated_641_residues
MTVSKILKQIIDSQTTKNVVIGQLGLGSITKLKQLITLLAHHKDKQLTLKLFVDISDTSGILLANEELTTLLCPQGSISL
TAIEGCQRVIVSNCKLTIDFYLGQYLTQLQALPMVNDGFVDGWHVTSDADQTQLRQILFWQLAKLSKNDAQFSLDDTFTE
VSQLELYTQAEQVGLYRYADNIPGNIQSGDEICFQERAAMRAQSRALQAPYPICSAAVTTHATCSNLGIAIIGGGVASAC
LALSLAERGQQVTLFCEDHALAQAASGNKQGAIYPLLTPDNNTLSQYFQQAYLFSLQRLKSLAAQGHPIDFDLCGVVHTG
HDERSRKRVAKIINGQNWQPSIARAITAEQASSIAGLKIDDGGIFYPMGGWVSPQDFTRAAFNQAKAIAGASLKLNTQIT
DIHYKDGGWELTSNTERFGSFKALILANGKSITQFPQTQYLQATGFRGQVSHVPSRAKLSKLSSVLCAHGYMTPSNNTLH
CLGASYVKNAPNTDYCPNEQVENLHKIQHSYVGQEWVEDIDVSGHSARVGVRMVTRDHAPMMGPAPDIDSIMTLYQDHQL
TPQSRKYWQSHNAPVHQGLYVLGGLGSRGLSSGPLAAESLAAQICGDLMPISRDFVALLNPNRMWMRKLLKGKALEVGVE
V
>Mature_640_residues
TVSKILKQIIDSQTTKNVVIGQLGLGSITKLKQLITLLAHHKDKQLTLKLFVDISDTSGILLANEELTTLLCPQGSISLT
AIEGCQRVIVSNCKLTIDFYLGQYLTQLQALPMVNDGFVDGWHVTSDADQTQLRQILFWQLAKLSKNDAQFSLDDTFTEV
SQLELYTQAEQVGLYRYADNIPGNIQSGDEICFQERAAMRAQSRALQAPYPICSAAVTTHATCSNLGIAIIGGGVASACL
ALSLAERGQQVTLFCEDHALAQAASGNKQGAIYPLLTPDNNTLSQYFQQAYLFSLQRLKSLAAQGHPIDFDLCGVVHTGH
DERSRKRVAKIINGQNWQPSIARAITAEQASSIAGLKIDDGGIFYPMGGWVSPQDFTRAAFNQAKAIAGASLKLNTQITD
IHYKDGGWELTSNTERFGSFKALILANGKSITQFPQTQYLQATGFRGQVSHVPSRAKLSKLSSVLCAHGYMTPSNNTLHC
LGASYVKNAPNTDYCPNEQVENLHKIQHSYVGQEWVEDIDVSGHSARVGVRMVTRDHAPMMGPAPDIDSIMTLYQDHQLT
PQSRKYWQSHNAPVHQGLYVLGGLGSRGLSSGPLAAESLAAQICGDLMPISRDFVALLNPNRMWMRKLLKGKALEVGVEV

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=569, Percent_Identity=34.2706502636204, Blast_Score=303, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_SHEPA (A8H2Z3)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001501465.1
- ProteinModelPortal:   A8H2Z3
- STRING:   A8H2Z3
- GeneID:   5662002
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_1605
- NMPDR:   fig|398579.3.peg.1385
- eggNOG:   COG0665
- HOGENOM:   HBG677640
- OMA:   WLILPHL
- ProtClustDB:   CLSK2516783
- BioCyc:   SPEA398579:SPEA_1605-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO

EC number: =2.1.1.61

Molecular weight: Translated: 70020; Mature: 69888

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSKILKQIIDSQTTKNVVIGQLGLGSITKLKQLITLLAHHKDKQLTLKLFVDISDTSG
CCHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCC
ILLANEELTTLLCPQGSISLTAIEGCQRVIVSNCKLTIDFYLGQYLTQLQALPMVNDGFV
EEEECCCEEEEECCCCCEEEEHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCCCC
DGWHVTSDADQTQLRQILFWQLAKLSKNDAQFSLDDTFTEVSQLELYTQAEQVGLYRYAD
CCEEECCCCCHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHEECC
NIPGNIQSGDEICFQERAAMRAQSRALQAPYPICSAAVTTHATCSNLGIAIIGGGVASAC
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHCCCEEEEECHHHHHH
LALSLAERGQQVTLFCEDHALAQAASGNKQGAIYPLLTPDNNTLSQYFQQAYLFSLQRLK
HHHHHHHCCCEEEEEECCCHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
SLAAQGHPIDFDLCGVVHTGHDERSRKRVAKIINGQNWQPSIARAITAEQASSIAGLKID
HHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEC
DGGIFYPMGGWVSPQDFTRAAFNQAKAIAGASLKLNTQITDIHYKDGGWELTSNTERFGS
CCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCEEECCCCHHHCC
FKALILANGKSITQFPQTQYLQATGFRGQVSHVPSRAKLSKLSSVLCAHGYMTPSNNTLH
EEEEEEECCCCCCCCCCHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHCCEECCCCCEEE
CLGASYVKNAPNTDYCPNEQVENLHKIQHSYVGQEWVEDIDVSGHSARVGVRMVTRDHAP
EECHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEEEECCCCC
MMGPAPDIDSIMTLYQDHQLTPQSRKYWQSHNAPVHQGLYVLGGLGSRGLSSGPLAAESL
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCHHCCEEEEECCCCCCCCCCCCHHHHH
AAQICGDLMPISRDFVALLNPNRMWMRKLLKGKALEVGVEV
HHHHHHCCCCCCCCEEEEECCCHHHHHHHHCCCEEEEECCC
>Mature Secondary Structure 
TVSKILKQIIDSQTTKNVVIGQLGLGSITKLKQLITLLAHHKDKQLTLKLFVDISDTSG
CHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCC
ILLANEELTTLLCPQGSISLTAIEGCQRVIVSNCKLTIDFYLGQYLTQLQALPMVNDGFV
EEEECCCEEEEECCCCCEEEEHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCCCC
DGWHVTSDADQTQLRQILFWQLAKLSKNDAQFSLDDTFTEVSQLELYTQAEQVGLYRYAD
CCEEECCCCCHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHEECC
NIPGNIQSGDEICFQERAAMRAQSRALQAPYPICSAAVTTHATCSNLGIAIIGGGVASAC
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHCCCEEEEECHHHHHH
LALSLAERGQQVTLFCEDHALAQAASGNKQGAIYPLLTPDNNTLSQYFQQAYLFSLQRLK
HHHHHHHCCCEEEEEECCCHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
SLAAQGHPIDFDLCGVVHTGHDERSRKRVAKIINGQNWQPSIARAITAEQASSIAGLKID
HHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEC
DGGIFYPMGGWVSPQDFTRAAFNQAKAIAGASLKLNTQITDIHYKDGGWELTSNTERFGS
CCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCEEECCCCHHHCC
FKALILANGKSITQFPQTQYLQATGFRGQVSHVPSRAKLSKLSSVLCAHGYMTPSNNTLH
EEEEEEECCCCCCCCCCHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHCCEECCCCCEEE
CLGASYVKNAPNTDYCPNEQVENLHKIQHSYVGQEWVEDIDVSGHSARVGVRMVTRDHAP
EECHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEEEECCCCC
MMGPAPDIDSIMTLYQDHQLTPQSRKYWQSHNAPVHQGLYVLGGLGSRGLSSGPLAAESL
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCHHCCEEEEECCCCCCCCCCCCHHHHH
AAQICGDLMPISRDFVALLNPNRMWMRKLLKGKALEVGVEV
HHHHHHCCCCCCCCEEEEECCCHHHHHHHHCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA