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The map label for this gene is cysB [H]

Identifier: 157961363

GI number: 157961363

Start: 1870985

End: 1871959

Strand: Direct

Name: cysB [H]

Synonym: Spea_1537

Alternate gene names: 157961363

Gene position: 1870985-1871959 (Clockwise)

Preceding gene: 157961361

Following gene: 157961365

Centisome position: 36.16

GC content: 44.31

Gene sequence:

>975_bases
ATGAAGTTGCAGCAACTTAGATATATTGCTGAGGTTGTAAATTACAATCTCAATGTTTCTGCTACTGCAGAAAATCTGTA
TACATCTCAGCCGGGTATAAGCAAGCAAGTAAGAATGCTTGAAGATGAACTTGGGATCCAGATTTTTGGCCGCAGTGGTA
AACATTTAACCCATGTTACGCCTGCTGGCCAGCAAGTGATCAATATCGCCAACGACATCTTAGGAAAAGTTGAAAGTATT
AAGAAAGTGGCTGAAGAGTATACTAAGCCAGATCAAGGCGAGCTCAACATCGCGACCACAGATACTCAAGCAAGATATGC
TTTGCCGCATATCATCCAAGGTTTTATTGATCGCTATCCAAAAGTTAACTTGCACATGCATCAAGGCACGCCATCACAGA
TCAGTGAGTTGGCCGCGAGAGGTGACGCTGATTTTGCTATAGCGACTGAAGCTATGCACCTCTATAGTGACTTGATCATG
TTGCCTTGTTATCACTGGAATCGTTCGATTGTGGTTACGCGTGATCATCCGCTGGCGGCGAGAAGCAATATTAGTATCGA
AGATCTCGCACGCTTCCCATTGGTCACTTATGTATTTGGCTTTGACCGTGCTTCAGAAATCGAAAAGTCCTTTAATCGCG
CTGGGCTAGAGCCAAGAGTGGTCTTCAGTGCGACCAGTGCCGATGTACTTAAAACCTATGTGAGACTTGGGTTAGGGGTG
GGTGTGATCGCTTCTATGGCGATTGACGAGCATATGGATAAAGATTTAGTGGCAATTGATGCAAGCCATCTATTTGCCCA
TAGCACCACTAAGATTGGCTTCAGAAAGGGTAACTTCCTGCGTACTTATATGTATGACTTTATAGAACATTTTGCGCCAC
ACTTGACTAGACCTGTAGTTGAGCAAGCCGTTGCACTGAGAGACGCACAGCAGATTGAAGCTATGTTTGAAGATATAGAA
CTTCCCGTTAGGTAG

Upstream 100 bases:

>100_bases
TTTAATTATAGCGTTGTACTATAACCAATAATTATACTCGAATGGATCATAACTCATATATTTAGACAGGTTTTTAGCCT
GCCCATCACAAGGATATAAG

Downstream 100 bases:

>100_bases
TTATACTGAGAGCTGTCACTGATAATAAAAAACCTCGCAATAGCGAGGTTTTTGTTTTTAAGTGCTTTGGTAAAAAGTGA
GCCCAAAGACAGTAATTAGC

Product: transcriptional regulator CysB

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKLQQLRYIAEVVNYNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVTPAGQQVINIANDILGKVESI
KKVAEEYTKPDQGELNIATTDTQARYALPHIIQGFIDRYPKVNLHMHQGTPSQISELAARGDADFAIATEAMHLYSDLIM
LPCYHWNRSIVVTRDHPLAARSNISIEDLARFPLVTYVFGFDRASEIEKSFNRAGLEPRVVFSATSADVLKTYVRLGLGV
GVIASMAIDEHMDKDLVAIDASHLFAHSTTKIGFRKGNFLRTYMYDFIEHFAPHLTRPVVEQAVALRDAQQIEAMFEDIE
LPVR

Sequences:

>Translated_324_residues
MKLQQLRYIAEVVNYNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVTPAGQQVINIANDILGKVESI
KKVAEEYTKPDQGELNIATTDTQARYALPHIIQGFIDRYPKVNLHMHQGTPSQISELAARGDADFAIATEAMHLYSDLIM
LPCYHWNRSIVVTRDHPLAARSNISIEDLARFPLVTYVFGFDRASEIEKSFNRAGLEPRVVFSATSADVLKTYVRLGLGV
GVIASMAIDEHMDKDLVAIDASHLFAHSTTKIGFRKGNFLRTYMYDFIEHFAPHLTRPVVEQAVALRDAQQIEAMFEDIE
LPVR
>Mature_324_residues
MKLQQLRYIAEVVNYNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVTPAGQQVINIANDILGKVESI
KKVAEEYTKPDQGELNIATTDTQARYALPHIIQGFIDRYPKVNLHMHQGTPSQISELAARGDADFAIATEAMHLYSDLIM
LPCYHWNRSIVVTRDHPLAARSNISIEDLARFPLVTYVFGFDRASEIEKSFNRAGLEPRVVFSATSADVLKTYVRLGLGV
GVIASMAIDEHMDKDLVAIDASHLFAHSTTKIGFRKGNFLRTYMYDFIEHFAPHLTRPVVEQAVALRDAQQIEAMFEDIE
LPVR

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=322, Percent_Identity=71.7391304347826, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI1788296, Length=289, Percent_Identity=40.4844290657439, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI1790399, Length=286, Percent_Identity=25.5244755244755, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI157672245, Length=242, Percent_Identity=29.3388429752066, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788887, Length=245, Percent_Identity=27.7551020408163, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790262, Length=240, Percent_Identity=24.5833333333333, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787879, Length=249, Percent_Identity=26.9076305220884, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789204, Length=240, Percent_Identity=22.9166666666667, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36368; Mature: 36368

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQQLRYIAEVVNYNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVT
CCHHHHHHHHHHHCCCCCEEEEHHHHCCCCCCHHHHHHHHHHHCCEEEECCCCCEEEEEC
PAGQQVINIANDILGKVESIKKVAEEYTKPDQGELNIATTDTQARYALPHIIQGFIDRYP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCC
KVNLHMHQGTPSQISELAARGDADFAIATEAMHLYSDLIMLPCYHWNRSIVVTRDHPLAA
CCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEECCCEEEEECCCCCCC
RSNISIEDLARFPLVTYVFGFDRASEIEKSFNRAGLEPRVVFSATSADVLKTYVRLGLGV
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHH
GVIASMAIDEHMDKDLVAIDASHLFAHSTTKIGFRKGNFLRTYMYDFIEHFAPHLTRPVV
HHHHHHHHHHHCCCCEEEEEHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
EQAVALRDAQQIEAMFEDIELPVR
HHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLQQLRYIAEVVNYNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVT
CCHHHHHHHHHHHCCCCCEEEEHHHHCCCCCCHHHHHHHHHHHCCEEEECCCCCEEEEEC
PAGQQVINIANDILGKVESIKKVAEEYTKPDQGELNIATTDTQARYALPHIIQGFIDRYP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCC
KVNLHMHQGTPSQISELAARGDADFAIATEAMHLYSDLIMLPCYHWNRSIVVTRDHPLAA
CCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEECCCEEEEECCCCCCC
RSNISIEDLARFPLVTYVFGFDRASEIEKSFNRAGLEPRVVFSATSADVLKTYVRLGLGV
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHH
GVIASMAIDEHMDKDLVAIDASHLFAHSTTKIGFRKGNFLRTYMYDFIEHFAPHLTRPVV
HHHHHHHHHHHCCCCEEEEEHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
EQAVALRDAQQIEAMFEDIELPVR
HHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]