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The map label for this gene is dusC [H]

Identifier: 157961327

GI number: 157961327

Start: 1822390

End: 1823349

Strand: Reverse

Name: dusC [H]

Synonym: Spea_1501

Alternate gene names: 157961327

Gene position: 1823349-1822390 (Counterclockwise)

Preceding gene: 157961336

Following gene: 157961326

Centisome position: 35.24

GC content: 48.02

Gene sequence:

>960_bases
ATGTTGGACTGTTCAGTGCGAATAATTTTGGCGCCTATGGAAGGCGTTGTAGATGATTTAATGCGAGAGATCCTCTCTGC
CATCAACCCTTATGATCTCATGGTCACCGAGTTTGTTCGTGTGGTGGATCAACTGCTGCCTGAAAAAGTGTTCCATCGCC
TTTGCCCTGAGCTGCAGACCGGTGGTCAAACATTGTCGGGCACGCCTGTTCGAGTGCAGCTACTGGGCCAAAATCCTGAC
TGGATGGCAGAAAACGCTGTAAGGGCAATTGAACTGGGCTCAAATGGTGTGGATGTGAATTTTGGCTGCCCCGCAAAGAT
GGTTAATCGTAGCAATGGCGGCGCCGTCATGCTGCAATACCCTGAGCAACTCTATAACGTTGTTAAAGCGATGCGTGATG
CAGTGCCAAGCGAGCAGCCTGTTACGGCAAAGATACGTCTAGGCTTTAACGATAAGTCGTTATATATGGAGAACGCCCTT
GCCATTTATGAAGCAGGCGCAAGTGAACTTGCAGTCCATGCTCGCAGCAAGGTCGATGGCTATAAGCCTCCTGCTTATTG
GGAATATATCACCGACATCAATGCCAAGTTACCCATCCCCGTCATTGCCAATGGCGAGATCTGGAATCGAGATGATGCCG
AGCGCTGTATGAGCGTAACTGGGTGTGACAATATCATGGTCGGGCGCGGAGCCATTTCACTGCCAAACCTTGCCGCGCAC
ATTAAAGGCGATGACACTTACACTTGGGAACAATCACTCAATCTCATGCTTGAATATACTAATCGCGAATTAGAGGGAAG
AAAGAGTACTTATTACCCGGCTCGGATCAAACAGTGGTTCGGTTATCTGAATCGTCAATATAGCGAAGCTGATGAACTAT
TTAGAGAGCTAAGAGTGTTTAAAACGGCGGAAGAGATTATCGACGTGCTACAACGTGCACAAGACAATTTAGCCAAATAA

Upstream 100 bases:

>100_bases
CACAATAAAGTTGTCATTGATGGGCTGTAGATAAAATGATTAAGTCTATTTGGCCGGCTGTTTAGGATGAGGTAGAATGC
AGCGCCTTTAAAATACCGAT

Downstream 100 bases:

>100_bases
CTAGCATTTTATTGCGCTATATCGGCAAAATATAGCGCTGAATTGATCTTAGTCATAACGCATCACGCCTGCAGGTTTAG
ACTAACAAGCCGTAAAGTCA

Product: dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MLDCSVRIILAPMEGVVDDLMREILSAINPYDLMVTEFVRVVDQLLPEKVFHRLCPELQTGGQTLSGTPVRVQLLGQNPD
WMAENAVRAIELGSNGVDVNFGCPAKMVNRSNGGAVMLQYPEQLYNVVKAMRDAVPSEQPVTAKIRLGFNDKSLYMENAL
AIYEAGASELAVHARSKVDGYKPPAYWEYITDINAKLPIPVIANGEIWNRDDAERCMSVTGCDNIMVGRGAISLPNLAAH
IKGDDTYTWEQSLNLMLEYTNRELEGRKSTYYPARIKQWFGYLNRQYSEADELFRELRVFKTAEEIIDVLQRAQDNLAK

Sequences:

>Translated_319_residues
MLDCSVRIILAPMEGVVDDLMREILSAINPYDLMVTEFVRVVDQLLPEKVFHRLCPELQTGGQTLSGTPVRVQLLGQNPD
WMAENAVRAIELGSNGVDVNFGCPAKMVNRSNGGAVMLQYPEQLYNVVKAMRDAVPSEQPVTAKIRLGFNDKSLYMENAL
AIYEAGASELAVHARSKVDGYKPPAYWEYITDINAKLPIPVIANGEIWNRDDAERCMSVTGCDNIMVGRGAISLPNLAAH
IKGDDTYTWEQSLNLMLEYTNRELEGRKSTYYPARIKQWFGYLNRQYSEADELFRELRVFKTAEEIIDVLQRAQDNLAK
>Mature_319_residues
MLDCSVRIILAPMEGVVDDLMREILSAINPYDLMVTEFVRVVDQLLPEKVFHRLCPELQTGGQTLSGTPVRVQLLGQNPD
WMAENAVRAIELGSNGVDVNFGCPAKMVNRSNGGAVMLQYPEQLYNVVKAMRDAVPSEQPVTAKIRLGFNDKSLYMENAL
AIYEAGASELAVHARSKVDGYKPPAYWEYITDINAKLPIPVIANGEIWNRDDAERCMSVTGCDNIMVGRGAISLPNLAAH
IKGDDTYTWEQSLNLMLEYTNRELEGRKSTYYPARIKQWFGYLNRQYSEADELFRELRVFKTAEEIIDVLQRAQDNLAK

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=201, Percent_Identity=32.3383084577114, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI239788462, Length=201, Percent_Identity=32.3383084577114, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI40807366, Length=332, Percent_Identity=28.6144578313253, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI31742496, Length=174, Percent_Identity=31.6091954022989, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI8923374, Length=241, Percent_Identity=27.8008298755187, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1788462, Length=308, Percent_Identity=52.9220779220779, Blast_Score=340, Evalue=9e-95,
Organism=Escherichia coli, GI1789660, Length=323, Percent_Identity=30.3405572755418, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI145693211, Length=250, Percent_Identity=26.8, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17543114, Length=218, Percent_Identity=27.5229357798165, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17507177, Length=171, Percent_Identity=32.7485380116959, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25144369, Length=224, Percent_Identity=27.2321428571429, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=173, Percent_Identity=30.0578034682081, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323437, Length=160, Percent_Identity=28.75, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24585320, Length=233, Percent_Identity=29.1845493562232, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24580595, Length=186, Percent_Identity=30.1075268817204, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19920448, Length=186, Percent_Identity=30.1075268817204, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19921524, Length=172, Percent_Identity=28.4883720930233, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI45549423, Length=156, Percent_Identity=27.5641025641026, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35915; Mature: 35915

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDCSVRIILAPMEGVVDDLMREILSAINPYDLMVTEFVRVVDQLLPEKVFHRLCPELQT
CCCCEEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHC
GGQTLSGTPVRVQLLGQNPDWMAENAVRAIELGSNGVDVNFGCPAKMVNRSNGGAVMLQY
CCCCCCCCCEEEEEECCCCCHHHHHCEEEEEECCCCEEEECCCCHHHHCCCCCCEEEEEC
PEQLYNVVKAMRDAVPSEQPVTAKIRLGFNDKSLYMENALAIYEAGASELAVHARSKVDG
HHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCC
YKPPAYWEYITDINAKLPIPVIANGEIWNRDDAERCMSVTGCDNIMVGRGAISLPNLAAH
CCCCHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCEEE
IKGDDTYTWEQSLNLMLEYTNRELEGRKSTYYPARIKQWFGYLNRQYSEADELFRELRVF
ECCCCCEEHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTAEEIIDVLQRAQDNLAK
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDCSVRIILAPMEGVVDDLMREILSAINPYDLMVTEFVRVVDQLLPEKVFHRLCPELQT
CCCCEEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHC
GGQTLSGTPVRVQLLGQNPDWMAENAVRAIELGSNGVDVNFGCPAKMVNRSNGGAVMLQY
CCCCCCCCCEEEEEECCCCCHHHHHCEEEEEECCCCEEEECCCCHHHHCCCCCCEEEEEC
PEQLYNVVKAMRDAVPSEQPVTAKIRLGFNDKSLYMENALAIYEAGASELAVHARSKVDG
HHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCC
YKPPAYWEYITDINAKLPIPVIANGEIWNRDDAERCMSVTGCDNIMVGRGAISLPNLAAH
CCCCHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCEEE
IKGDDTYTWEQSLNLMLEYTNRELEGRKSTYYPARIKQWFGYLNRQYSEADELFRELRVF
ECCCCCEEHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTAEEIIDVLQRAQDNLAK
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]