Click here to switch to the map view.

The map label for this gene is btuB [H]

Identifier: 157961325

GI number: 157961325

Start: 1819107

End: 1821791

Strand: Direct

Name: btuB [H]

Synonym: Spea_1499

Alternate gene names: 157961325

Gene position: 1819107-1821791 (Clockwise)

Preceding gene: 157961324

Following gene: 157961328

Centisome position: 35.15

GC content: 43.17

Gene sequence:

>2685_bases
ATGAGCTCAGTAACCTTAACCGCAAAAGCAGTACGTCGTAGCTTTATAGCCGCTGCAGCGACAAGTGTTGCTTTATCTGG
ATTTGCATTTGCAGAAGAAGCTGCAGATGAAAAAGTAGAACGTATCGAAGTAACCGGTTCGCGTATTAAGCAAGTCGATA
TGGAAACTGTTTCTCCTGTTACAGTTATCAGTGCTGCTGATATCGCTATGACAGGTGAGAAAACAGTTGCAGATGTTTTA
AACAACTCTGCAGTAAATAGCTTTGGTTCTTGGCGTGGCATGTCTGGCTACGGCTCAGGCGGTAGCGCAACAAGTAGTGT
TAACCTGCGTGGTCTAGGTTCTTCTGCAACACTTGTTTTGCTAGACGGCCGTCGTATGCCGGGTACTAGCTCAAGTTCTG
GAGCGGTAGCCGATACATCTCAGATCCCAATGGCGATTGTTGAACGTATTGAAATTCTACGTGATGGTGCATCGGCTGTT
TATGGTTCTGATGCTGTTGCTGGTGTAATTAACATTATCACTAAGAAAGATTTCGATGGTGTTCAATTAGATTTTAGTAC
TGAGCAGCCAAGTATTGAAGGCGGTGATGCTAACCGTTTCTCATTAGCGACGGGTTTTAACACCGACAAAGGTAACATCA
CTTTTACTTATGAGTATTATGATACTAAGAACGTGATGGACCGTGATGTTTGGGACATGAATGACCCAACTTACAGCGAT
TATAGCTCTTTCAGCTCAGTACCTAACGGTTATTACAACACTGGCGAGAAGAATGATGATGGTAAAGACATCTATGCATT
CTATTCAAACTCAGATATGTGTGAGCAGACTGAAAACGTATCTAACGGTACCGATGGTGAGAATAACGGTCGTTGTTACT
ACAGCTATGGTGAAGTGACTAAGTTATTTGGTGATGTGACTCGTAACTCATTTATGACTAATTTTAGTTACGAAGTTGCA
GAAGATATTCAGTTCCGTGGTCGTGGTTCTGCATCATTTACTGAAACCCATACCCGTTATGCTGGTACGCCTGTATCGAC
AAACTACCCTGTGATGGACGCAGAAAACCAGTATAACCCTACTGGTGAAGATATGACTATCTACATGCGCTCAGTACAGA
TTGGTGAGCGTGACACACTGACTGAAACAAATAACTTCGATATCTTAGGCGGACTCGTTGGATTCAGTGATGTTGCTAAT
GGTGTTGATTGGGAAGTAAACCTACAGCATTCAGCTTCTACCACTAATTCATTTAACTATAATCTTATCAATGACAATAT
CATCCAGAGTGAAATTGACACAGGTGAGTACGACATCTTTAACACTCAAGGCTTAGCTTATGATGAGTGGGAACAGCAGA
TGGGAGCGCTATATGGCGCCGCTGCTCACACAGGTGTTTACCAGGGTAAATTTGAAAGTACACAAATTGACGCACTTGCT
TCAACATTAATTTTCGAAAATGATGCAGTATCACTAGCTATGGTTGGTGGTGTTGAATATGAAATGATTAGCTTTGAGCA
AACCAGTGATCCAGAGTCTGCTGCGGGTATAATCTCAGGTGGTTCAGGTGGTGATGATGTTGATGCAACACGTGACCGTA
CAGCAGGTTATGTAGAATTCCAGATGGGGCTGCCTGCCAATGTTGAGCTTTCAGCAGCTGTTCGTTATGAACGTTATGAG
CAAGAAGGTAACCTAACTGGTGCAACTGGTGAAGTGACTGAGTCATCTACATTTGATGCAGTAGTTCCTAAGTTTGGTTT
GAGCTGGCGCCCAGTAGATTCACTATTACTACGTGCGAGCTACGGTGATTCATTCCGCGCACCAAACATGGGCGAGATGT
TCTCTTCACAGTCACTAAGTTTCGAAAGCGGCTATGATCCAGTTTGGTGTGGTGCCAACGGCGACGATGCTGACGCAGAT
TACTGTTCACCAACCAACCAATATAAAACTTGGTACGGTGGTAACCCAGATCTAGAAGCTGAAGAAGGTAACTCGTTAAC
ACTTGGTGGTGTGTGGAACGTAACTGATGAGTGGAACATTGAGCTTTCTTACTATTCAATCGTTTATGATAATAAGATTG
AATCAGTCGGTATTGATGATCTTTTATATGATGAGCAGATCAATGGTGGTTCAGATAAGATTCATCGTGGAGCAGACGGT
AAGATTGAGTACATTGAAGCGGGTGTAGAAAACATCGCCTCAGTTGAAACATCAGGTCTAGATTTTGTGACAGCCTATAA
CCTAGAGACAGGTTTTGGTGACTTCAACTTCAAGCTAGATGTGTCACATGTAATGGCATTCGATAAGCAAGCCGATGCAA
CATCAGAAATGGTTGAGTATGCAGGTACTCAGGATTACCCAGATTGGCGTGGTAACTTCGCTGCTAGTTGGTACTTAAAC
GACTTCAGCGCTGCTTGGACAACTGTTTATATCGGTAGTCAGTCAGGTCAAGATCTTCGTGATGAAGGCTATGACTACAT
CGTTGACATACCTAGTTACATGAAGCACAACTTCCAGGTGGGTTACACCCATGATTGGAACGGCAGCATCACAGTTGGTG
TGAACAATATCTTTGATGAAGAAGCGCCAAGCATGTATGACTTCGCGGGTTACCGTGACACAAATACGGGTTTATATGAT
GTCTTAGGACGCACTTATTACCTAAGATTGAACCAGAAGTTCTAA

Upstream 100 bases:

>100_bases
TTTAAACAGTATTTATTACGAAGTGTTTAACTTCCTTTTAAAAATAATAACTAAATACAACTGTTCCGACGTTATAATAT
TGGAATATGAGGGGAAAAAT

Downstream 100 bases:

>100_bases
TCTTTTTGTAAATCAATAGATGAGTTAGCTAGATAGTAAAAAACCTCCGTCATGCGGAGGTTTTTTTATGGCTAAGTCAG
CAACATTGGCTGCTGGCGAG

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 894; Mature: 893

Protein sequence:

>894_residues
MSSVTLTAKAVRRSFIAAAATSVALSGFAFAEEAADEKVERIEVTGSRIKQVDMETVSPVTVISAADIAMTGEKTVADVL
NNSAVNSFGSWRGMSGYGSGGSATSSVNLRGLGSSATLVLLDGRRMPGTSSSSGAVADTSQIPMAIVERIEILRDGASAV
YGSDAVAGVINIITKKDFDGVQLDFSTEQPSIEGGDANRFSLATGFNTDKGNITFTYEYYDTKNVMDRDVWDMNDPTYSD
YSSFSSVPNGYYNTGEKNDDGKDIYAFYSNSDMCEQTENVSNGTDGENNGRCYYSYGEVTKLFGDVTRNSFMTNFSYEVA
EDIQFRGRGSASFTETHTRYAGTPVSTNYPVMDAENQYNPTGEDMTIYMRSVQIGERDTLTETNNFDILGGLVGFSDVAN
GVDWEVNLQHSASTTNSFNYNLINDNIIQSEIDTGEYDIFNTQGLAYDEWEQQMGALYGAAAHTGVYQGKFESTQIDALA
STLIFENDAVSLAMVGGVEYEMISFEQTSDPESAAGIISGGSGGDDVDATRDRTAGYVEFQMGLPANVELSAAVRYERYE
QEGNLTGATGEVTESSTFDAVVPKFGLSWRPVDSLLLRASYGDSFRAPNMGEMFSSQSLSFESGYDPVWCGANGDDADAD
YCSPTNQYKTWYGGNPDLEAEEGNSLTLGGVWNVTDEWNIELSYYSIVYDNKIESVGIDDLLYDEQINGGSDKIHRGADG
KIEYIEAGVENIASVETSGLDFVTAYNLETGFGDFNFKLDVSHVMAFDKQADATSEMVEYAGTQDYPDWRGNFAASWYLN
DFSAAWTTVYIGSQSGQDLRDEGYDYIVDIPSYMKHNFQVGYTHDWNGSITVGVNNIFDEEAPSMYDFAGYRDTNTGLYD
VLGRTYYLRLNQKF

Sequences:

>Translated_894_residues
MSSVTLTAKAVRRSFIAAAATSVALSGFAFAEEAADEKVERIEVTGSRIKQVDMETVSPVTVISAADIAMTGEKTVADVL
NNSAVNSFGSWRGMSGYGSGGSATSSVNLRGLGSSATLVLLDGRRMPGTSSSSGAVADTSQIPMAIVERIEILRDGASAV
YGSDAVAGVINIITKKDFDGVQLDFSTEQPSIEGGDANRFSLATGFNTDKGNITFTYEYYDTKNVMDRDVWDMNDPTYSD
YSSFSSVPNGYYNTGEKNDDGKDIYAFYSNSDMCEQTENVSNGTDGENNGRCYYSYGEVTKLFGDVTRNSFMTNFSYEVA
EDIQFRGRGSASFTETHTRYAGTPVSTNYPVMDAENQYNPTGEDMTIYMRSVQIGERDTLTETNNFDILGGLVGFSDVAN
GVDWEVNLQHSASTTNSFNYNLINDNIIQSEIDTGEYDIFNTQGLAYDEWEQQMGALYGAAAHTGVYQGKFESTQIDALA
STLIFENDAVSLAMVGGVEYEMISFEQTSDPESAAGIISGGSGGDDVDATRDRTAGYVEFQMGLPANVELSAAVRYERYE
QEGNLTGATGEVTESSTFDAVVPKFGLSWRPVDSLLLRASYGDSFRAPNMGEMFSSQSLSFESGYDPVWCGANGDDADAD
YCSPTNQYKTWYGGNPDLEAEEGNSLTLGGVWNVTDEWNIELSYYSIVYDNKIESVGIDDLLYDEQINGGSDKIHRGADG
KIEYIEAGVENIASVETSGLDFVTAYNLETGFGDFNFKLDVSHVMAFDKQADATSEMVEYAGTQDYPDWRGNFAASWYLN
DFSAAWTTVYIGSQSGQDLRDEGYDYIVDIPSYMKHNFQVGYTHDWNGSITVGVNNIFDEEAPSMYDFAGYRDTNTGLYD
VLGRTYYLRLNQKF
>Mature_893_residues
SSVTLTAKAVRRSFIAAAATSVALSGFAFAEEAADEKVERIEVTGSRIKQVDMETVSPVTVISAADIAMTGEKTVADVLN
NSAVNSFGSWRGMSGYGSGGSATSSVNLRGLGSSATLVLLDGRRMPGTSSSSGAVADTSQIPMAIVERIEILRDGASAVY
GSDAVAGVINIITKKDFDGVQLDFSTEQPSIEGGDANRFSLATGFNTDKGNITFTYEYYDTKNVMDRDVWDMNDPTYSDY
SSFSSVPNGYYNTGEKNDDGKDIYAFYSNSDMCEQTENVSNGTDGENNGRCYYSYGEVTKLFGDVTRNSFMTNFSYEVAE
DIQFRGRGSASFTETHTRYAGTPVSTNYPVMDAENQYNPTGEDMTIYMRSVQIGERDTLTETNNFDILGGLVGFSDVANG
VDWEVNLQHSASTTNSFNYNLINDNIIQSEIDTGEYDIFNTQGLAYDEWEQQMGALYGAAAHTGVYQGKFESTQIDALAS
TLIFENDAVSLAMVGGVEYEMISFEQTSDPESAAGIISGGSGGDDVDATRDRTAGYVEFQMGLPANVELSAAVRYERYEQ
EGNLTGATGEVTESSTFDAVVPKFGLSWRPVDSLLLRASYGDSFRAPNMGEMFSSQSLSFESGYDPVWCGANGDDADADY
CSPTNQYKTWYGGNPDLEAEEGNSLTLGGVWNVTDEWNIELSYYSIVYDNKIESVGIDDLLYDEQINGGSDKIHRGADGK
IEYIEAGVENIASVETSGLDFVTAYNLETGFGDFNFKLDVSHVMAFDKQADATSEMVEYAGTQDYPDWRGNFAASWYLND
FSAAWTTVYIGSQSGQDLRDEGYDYIVDIPSYMKHNFQVGYTHDWNGSITVGVNNIFDEEAPSMYDFAGYRDTNTGLYDV
LGRTYYLRLNQKF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=203, Percent_Identity=35.9605911330049, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1788478, Length=167, Percent_Identity=33.5329341317365, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI1786798, Length=162, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 97722; Mature: 97591

Theoretical pI: Translated: 3.88; Mature: 3.88

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVTLTAKAVRRSFIAAAATSVALSGFAFAEEAADEKVERIEVTGSRIKQVDMETVSPV
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHEECHHHCCCE
TVISAADIAMTGEKTVADVLNNSAVNSFGSWRGMSGYGSGGSATSSVNLRGLGSSATLVL
EEEEECCEEECCCHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEE
LDGRRMPGTSSSSGAVADTSQIPMAIVERIEILRDGASAVYGSDAVAGVINIITKKDFDG
EECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCC
VQLDFSTEQPSIEGGDANRFSLATGFNTDKGNITFTYEYYDTKNVMDRDVWDMNDPTYSD
EEEEECCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCC
YSSFSSVPNGYYNTGEKNDDGKDIYAFYSNSDMCEQTENVSNGTDGENNGRCYYSYGEVT
HHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCEEEEEHHHHH
KLFGDVTRNSFMTNFSYEVAEDIQFRGRGSASFTETHTRYAGTPVSTNYPVMDAENQYNP
HHHHHHHHHHEEECCCHHHHHCCEECCCCCCCCCCCCCEECCCCCCCCCCEECCCCCCCC
TGEDMTIYMRSVQIGERDTLTETNNFDILGGLVGFSDVANGVDWEVNLQHSASTTNSFNY
CCCCEEEEEEEEECCCCCCCCCCCCCEEEEHHHCHHHHCCCCCEEEEEECCCCCCCCEEE
NLINDNIIQSEIDTGEYDIFNTQGLAYDEWEQQMGALYGAAAHTGVYQGKFESTQIDALA
EEECCCHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHH
STLIFENDAVSLAMVGGVEYEMISFEQTSDPESAAGIISGGSGGDDVDATRDRTAGYVEF
HHHEECCCCEEEEEECCCEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEE
QMGLPANVELSAAVRYERYEQEGNLTGATGEVTESSTFDAVVPKFGLSWRPVDSLLLRAS
EECCCCCCEEHEEHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHEEEEC
YGDSFRAPNMGEMFSSQSLSFESGYDPVWCGANGDDADADYCSPTNQYKTWYGGNPDLEA
CCCCCCCCCHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCC
EEGNSLTLGGVWNVTDEWNIELSYYSIVYDNKIESVGIDDLLYDEQINGGSDKIHRGADG
CCCCEEEECEEEECCCCEEEEEEEEEEEECCCCCCCCCHHHHCCCCCCCCCCCEECCCCC
KIEYIEAGVENIASVETSGLDFVTAYNLETGFGDFNFKLDVSHVMAFDKQADATSEMVEY
CEEEEHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEEHHHHHHHCCCCCHHHHHHHH
AGTQDYPDWRGNFAASWYLNDFSAAWTTVYIGSQSGQDLRDEGYDYIVDIPSYMKHNFQV
CCCCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCHHHCCCEEEEECHHHHCCCEEE
GYTHDWNGSITVGVNNIFDEEAPSMYDFAGYRDTNTGLYDVLGRTYYLRLNQKF
EEEECCCCEEEEEECHHCCCCCCCCCCCCCCCCCCCCHHHHCCCEEEEEEECCC
>Mature Secondary Structure 
SSVTLTAKAVRRSFIAAAATSVALSGFAFAEEAADEKVERIEVTGSRIKQVDMETVSPV
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHEECHHHCCCE
TVISAADIAMTGEKTVADVLNNSAVNSFGSWRGMSGYGSGGSATSSVNLRGLGSSATLVL
EEEEECCEEECCCHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEE
LDGRRMPGTSSSSGAVADTSQIPMAIVERIEILRDGASAVYGSDAVAGVINIITKKDFDG
EECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCC
VQLDFSTEQPSIEGGDANRFSLATGFNTDKGNITFTYEYYDTKNVMDRDVWDMNDPTYSD
EEEEECCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCC
YSSFSSVPNGYYNTGEKNDDGKDIYAFYSNSDMCEQTENVSNGTDGENNGRCYYSYGEVT
HHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCEEEEEHHHHH
KLFGDVTRNSFMTNFSYEVAEDIQFRGRGSASFTETHTRYAGTPVSTNYPVMDAENQYNP
HHHHHHHHHHEEECCCHHHHHCCEECCCCCCCCCCCCCEECCCCCCCCCCEECCCCCCCC
TGEDMTIYMRSVQIGERDTLTETNNFDILGGLVGFSDVANGVDWEVNLQHSASTTNSFNY
CCCCEEEEEEEEECCCCCCCCCCCCCEEEEHHHCHHHHCCCCCEEEEEECCCCCCCCEEE
NLINDNIIQSEIDTGEYDIFNTQGLAYDEWEQQMGALYGAAAHTGVYQGKFESTQIDALA
EEECCCHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHH
STLIFENDAVSLAMVGGVEYEMISFEQTSDPESAAGIISGGSGGDDVDATRDRTAGYVEF
HHHEECCCCEEEEEECCCEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEE
QMGLPANVELSAAVRYERYEQEGNLTGATGEVTESSTFDAVVPKFGLSWRPVDSLLLRAS
EECCCCCCEEHEEHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHEEEEC
YGDSFRAPNMGEMFSSQSLSFESGYDPVWCGANGDDADADYCSPTNQYKTWYGGNPDLEA
CCCCCCCCCHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCC
EEGNSLTLGGVWNVTDEWNIELSYYSIVYDNKIESVGIDDLLYDEQINGGSDKIHRGADG
CCCCEEEECEEEECCCCEEEEEEEEEEEECCCCCCCCCHHHHCCCCCCCCCCCEECCCCC
KIEYIEAGVENIASVETSGLDFVTAYNLETGFGDFNFKLDVSHVMAFDKQADATSEMVEY
CEEEEHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEEHHHHHHHCCCCCHHHHHHHH
AGTQDYPDWRGNFAASWYLNDFSAAWTTVYIGSQSGQDLRDEGYDYIVDIPSYMKHNFQV
CCCCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCHHHCCCEEEEECHHHHCCCEEE
GYTHDWNGSITVGVNNIFDEEAPSMYDFAGYRDTNTGLYDVLGRTYYLRLNQKF
EEEECCCCEEEEEECHHCCCCCCCCCCCCCCCCCCCCHHHHCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA