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The map label for this gene is tgt [H]

Identifier: 157961296

GI number: 157961296

Start: 1793152

End: 1794285

Strand: Direct

Name: tgt [H]

Synonym: Spea_1470

Alternate gene names: 157961296

Gene position: 1793152-1794285 (Clockwise)

Preceding gene: 157961295

Following gene: 157961297

Centisome position: 34.65

GC content: 45.15

Gene sequence:

>1134_bases
ATGAAATTTGAATTAGATACAACTCAAGGCCGCGCACGTCGTGGTCGTTTGATTTTTGAACGTGGTACGGTTGAAACGCC
AGCGTTTATGCCTGTCGGTACTTACGGTACCGTTAAAGGTATGACGCCTGAAGAAGTGCGCGAAACTGGCGCAGACATTC
TGCTTGGTAATACTTTCCACCTTTGGTTACGTCCAGGTGAAGAGATTATGCGTAAGCATGGCGACTTGCATGACTTCATG
AATTGGCAACGTCCTATTTTGACCGACTCGGGTGGATTCCAGGTTTTTAGCTTGGGTGATATTCGTAAGATCACTGAAGA
AGGCGTTCATTTCCGCTCACCAATTAACGGTGAAAAGATTTTCTTGGACCCAGAAAAGTCGATGCAAATTCAAGACTCAT
TAGGCAGCGATGTAGTAATGATTTTTGACGAATGCACCCCGTACCCGGCAACTGAGGACGAAGCTCGTAAATCGATGCAG
ATGTCACTTCGTTGGGCGCAGCGTTCACGCGATGAGTTTGACAGACTTGAAAACCCGAACTCTTTGTTCGGTATTATTCA
AGGCGGTGTTTATGAAGACCTTCGTGACGAAAGCCTAAATGGCTTAGTCGACATTGGTTTTGATGGATACGCTGTTGGTG
GTTTAGCAGTTGGCGAGCCAAAGGAAGACATGCACCGCATTCTTGAGCATGTTTGTCCAAAAATTCCAGCTGATAAACCT
CGTTATTTGATGGGGGTCGGTAAGCCAGAAGATTTAGTTGAAGGCGTACGTCGCGGCGTGGACATGTTCGATTGTGTTAT
GCCAACACGAAATGCTCGTAACGGTCATCTGTTTACCAGTGAAGGTGTTATTAAGATCCGTAATGCACGTCATCGCGATG
ATACTTCACCACTCGATGATAAGTGTGATTGTTATACCTGTAAGAACTACTCTCGGGCATACCTTTACCACTTAGATCGT
TGTAATGAGATTCTAGGTGCTCGATTAAACACCATTCATAACCTTCGCTATTACCAAAGATTGATGGAAGGTTTGCGTGG
CGCTATCGAGACAGGTACATTAGACGCCTTTGTTACGGAATTCTATACCAGCCAAGGTCGAGAAGTACCTGAAGTTCCTG
AATTATCCGATTAA

Upstream 100 bases:

>100_bases
TAGGGTTGATACTGAATTGTTTGCCCTTATGTTTGTCATAATCTTAGACTCAAATACTTGAGTTAACTCAGGTCAGACTG
TTTATCTGGCGAGGTGAAAA

Downstream 100 bases:

>100_bases
TTTTTACTCAGACAATTAGAAGAGAATACTATGTTTATTTCAAACGCATATGCAGCTGATGCACCTGTTGGTGGCGCTGG
TGGTACGATGGAACTGATTT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MKFELDTTQGRARRGRLIFERGTVETPAFMPVGTYGTVKGMTPEEVRETGADILLGNTFHLWLRPGEEIMRKHGDLHDFM
NWQRPILTDSGGFQVFSLGDIRKITEEGVHFRSPINGEKIFLDPEKSMQIQDSLGSDVVMIFDECTPYPATEDEARKSMQ
MSLRWAQRSRDEFDRLENPNSLFGIIQGGVYEDLRDESLNGLVDIGFDGYAVGGLAVGEPKEDMHRILEHVCPKIPADKP
RYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFTSEGVIKIRNARHRDDTSPLDDKCDCYTCKNYSRAYLYHLDR
CNEILGARLNTIHNLRYYQRLMEGLRGAIETGTLDAFVTEFYTSQGREVPEVPELSD

Sequences:

>Translated_377_residues
MKFELDTTQGRARRGRLIFERGTVETPAFMPVGTYGTVKGMTPEEVRETGADILLGNTFHLWLRPGEEIMRKHGDLHDFM
NWQRPILTDSGGFQVFSLGDIRKITEEGVHFRSPINGEKIFLDPEKSMQIQDSLGSDVVMIFDECTPYPATEDEARKSMQ
MSLRWAQRSRDEFDRLENPNSLFGIIQGGVYEDLRDESLNGLVDIGFDGYAVGGLAVGEPKEDMHRILEHVCPKIPADKP
RYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFTSEGVIKIRNARHRDDTSPLDDKCDCYTCKNYSRAYLYHLDR
CNEILGARLNTIHNLRYYQRLMEGLRGAIETGTLDAFVTEFYTSQGREVPEVPELSD
>Mature_377_residues
MKFELDTTQGRARRGRLIFERGTVETPAFMPVGTYGTVKGMTPEEVRETGADILLGNTFHLWLRPGEEIMRKHGDLHDFM
NWQRPILTDSGGFQVFSLGDIRKITEEGVHFRSPINGEKIFLDPEKSMQIQDSLGSDVVMIFDECTPYPATEDEARKSMQ
MSLRWAQRSRDEFDRLENPNSLFGIIQGGVYEDLRDESLNGLVDIGFDGYAVGGLAVGEPKEDMHRILEHVCPKIPADKP
RYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFTSEGVIKIRNARHRDDTSPLDDKCDCYTCKNYSRAYLYHLDR
CNEILGARLNTIHNLRYYQRLMEGLRGAIETGTLDAFVTEFYTSQGREVPEVPELSD

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=361, Percent_Identity=43.4903047091413, Blast_Score=307, Evalue=1e-83,
Organism=Escherichia coli, GI1786607, Length=370, Percent_Identity=82.4324324324324, Blast_Score=650, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544680, Length=379, Percent_Identity=41.1609498680739, Blast_Score=297, Evalue=6e-81,
Organism=Caenorhabditis elegans, GI25146502, Length=165, Percent_Identity=31.5151515151515, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19920510, Length=369, Percent_Identity=42.0054200542005, Blast_Score=310, Evalue=7e-85,
Organism=Drosophila melanogaster, GI21355311, Length=255, Percent_Identity=25.8823529411765, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42913; Mature: 42913

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFELDTTQGRARRGRLIFERGTVETPAFMPVGTYGTVKGMTPEEVRETGADILLGNTFH
CEEECCCCCCHHHCCCEEEECCCCCCCCCEECCCCCCCCCCCHHHHHHCCCCEEECCEEE
LWLRPGEEIMRKHGDLHDFMNWQRPILTDSGGFQVFSLGDIRKITEEGVHFRSPINGEKI
EEECCCHHHHHHCCCHHHHHCCCCCEEECCCCEEEEECCHHHHHHHCCCEECCCCCCCEE
FLDPEKSMQIQDSLGSDVVMIFDECTPYPATEDEARKSMQMSLRWAQRSRDEFDRLENPN
EECCCCCCHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SLFGIIQGGVYEDLRDESLNGLVDIGFDGYAVGGLAVGEPKEDMHRILEHVCPKIPADKP
CEEEECCCCHHHHHHHHCCCCEEEECCCCEEECCEECCCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFTSEGVIKIRNARHRDDTSPLDD
CEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEECCCEEEECCCCCCCCCCCCCC
KCDCYTCKNYSRAYLYHLDRCNEILGARLNTIHNLRYYQRLMEGLRGAIETGTLDAFVTE
CCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
FYTSQGREVPEVPELSD
HHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKFELDTTQGRARRGRLIFERGTVETPAFMPVGTYGTVKGMTPEEVRETGADILLGNTFH
CEEECCCCCCHHHCCCEEEECCCCCCCCCEECCCCCCCCCCCHHHHHHCCCCEEECCEEE
LWLRPGEEIMRKHGDLHDFMNWQRPILTDSGGFQVFSLGDIRKITEEGVHFRSPINGEKI
EEECCCHHHHHHCCCHHHHHCCCCCEEECCCCEEEEECCHHHHHHHCCCEECCCCCCCEE
FLDPEKSMQIQDSLGSDVVMIFDECTPYPATEDEARKSMQMSLRWAQRSRDEFDRLENPN
EECCCCCCHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SLFGIIQGGVYEDLRDESLNGLVDIGFDGYAVGGLAVGEPKEDMHRILEHVCPKIPADKP
CEEEECCCCHHHHHHHHCCCCEEEECCCCEEECCEECCCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFTSEGVIKIRNARHRDDTSPLDD
CEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEECCCEEEECCCCCCCCCCCCCC
KCDCYTCKNYSRAYLYHLDRCNEILGARLNTIHNLRYYQRLMEGLRGAIETGTLDAFVTE
CCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
FYTSQGREVPEVPELSD
HHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA