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The map label for this gene is luxO [H]

Identifier: 157961261

GI number: 157961261

Start: 1746558

End: 1748054

Strand: Direct

Name: luxO [H]

Synonym: Spea_1435

Alternate gene names: 157961261

Gene position: 1746558-1748054 (Clockwise)

Preceding gene: 157961260

Following gene: 157961262

Centisome position: 33.75

GC content: 46.16

Gene sequence:

>1497_bases
ATGGATATAGCAAGCTCATTTACCACCTTGCTCGTCGAAGACAGTATGTCTCTCGGGGCGCTTTATACAGAATACCTTCG
CACGGAGGGGGCGCGAGTCACTCATGTTAATCATGGGAGTGATGCGTTGAATGAGCTTAAACGCTGGCAACCGGATCTAT
TAGTGCTCGATATTCAGCTACCAGATATGTCTGGGATGGATATTTTGGAGATCGTACAGTCAGAGTACCCAGATGTTACC
GTCATAATGATCACTGCCCACGGCAGCATTGATATCGCTGTCGATGCCATGCGCTCGGGGGCGTTTGATTTTCTTATTAA
ACCTTTCGATGCAAAACGTTTATCCATTACCGTACGTAATGCATTAAAACAGCGTCAGCTTGTTAATCTTGTCGCCAAGT
ACGAGAGTAGCCTGCCTAAGCCACATTATATGGGGTTCATTGGAGAGTCGTTGGCGATGCAAACCGTATATAAAACTATC
GATTGTGTAGCGAGCAGCAAAGCTTCGGCCTTTATCATTGGTGAAAGTGGTACGGGTAAAGAGGTGTGTGCTCATGCGAT
ACACAATGCGGGTAACCGTAGCGATGGGCCATTTGTGGCTTTAAACTGTGCGTCAATCCCAAAGGATTTGATTGAAAGTG
AGATTTTTGGTCACACCAAAGGGGCGTTTACTGGCGCGATAGCCAATAGAGATGGCGCGGCGACACGGGCGCATAAGGGC
ACGCTTTTTCTTGATGAGATCTGCGAGATGGATTTGGAGCTGCAGAGTAAGCTACTGCGCTTTATTCAAACCGGTGTGTT
TCAACGTGTTGGGGCTACAAAAGAAGAAAAGGTCGATGTACGCTTTGTCAGTGCGACTAACCGTATGCCTTGGGATGAAG
TTAAAGCGGGGCGTTTTAGAGAGGATCTATTCTACCGTTTACATGTGATCCCAATTGAGCTGCCGCCGCTGAGAATGCGC
GGCAAAGATATTTTGCTGCTGGCATCCTCTTTACTCAAAGAGTACAACAAAGAAGAGGGTAAGACGTTTAAGGGATTTAG
CCCTGAAGCTAAGCAATGCTTAAAGTCTTACCCATGGCCCGGTAATGTTAGGCAATTACAGAACGTTATTAGGCAGATAG
TGGTATTGAATGACAGCGAAACGATTGAGATTGATATGTTACCAATACAGCTGACATCGACTTCAGCGATTAAATCTGAA
GTCGCTAAGCCACAGGCGGCGGTTGTTAAGTCTCAGTCTCTCCGAGGTGACTCTGACGCCTCCTCTTTTCTTGATGGAAT
AGAGCGCGCGGCTTTTTCTGGTTCCGAGCAATATGCTAAATCTGCCAATGAAAAAAGTGATGATATTGTACCGCTATGGA
AGACAGAGAAGCAGACCATTGAAAATGCTATAGCACGTTGTGATGGCAATGTGCCTAAGGCCGCAGCATTATTGGATATC
AGTGCATCGACTATCTATCGAAAACGCCAGTCATGGGATGAGTTAGAAAAAGCTTAA

Upstream 100 bases:

>100_bases
GCTAGACTTAGCAATATGCTCATGCGAGTCATGCTAGTTTAATTCTTTGATATTAGGCAAGTTATCGCGATAGTTGTTCT
TATTAATAGGCTGACCTTAT

Downstream 100 bases:

>100_bases
GTCACTAAAACCTTAACTAATGTTTTAGTATTAAGTAATCAAGCTTATCGATAGAGCTCAGCAAATGAAAAGAGTAGACT
GAGGGTCTACTCTTTTTTGT

Product: Fis family two component sigma54 specific transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MDIASSFTTLLVEDSMSLGALYTEYLRTEGARVTHVNHGSDALNELKRWQPDLLVLDIQLPDMSGMDILEIVQSEYPDVT
VIMITAHGSIDIAVDAMRSGAFDFLIKPFDAKRLSITVRNALKQRQLVNLVAKYESSLPKPHYMGFIGESLAMQTVYKTI
DCVASSKASAFIIGESGTGKEVCAHAIHNAGNRSDGPFVALNCASIPKDLIESEIFGHTKGAFTGAIANRDGAATRAHKG
TLFLDEICEMDLELQSKLLRFIQTGVFQRVGATKEEKVDVRFVSATNRMPWDEVKAGRFREDLFYRLHVIPIELPPLRMR
GKDILLLASSLLKEYNKEEGKTFKGFSPEAKQCLKSYPWPGNVRQLQNVIRQIVVLNDSETIEIDMLPIQLTSTSAIKSE
VAKPQAAVVKSQSLRGDSDASSFLDGIERAAFSGSEQYAKSANEKSDDIVPLWKTEKQTIENAIARCDGNVPKAAALLDI
SASTIYRKRQSWDELEKA

Sequences:

>Translated_498_residues
MDIASSFTTLLVEDSMSLGALYTEYLRTEGARVTHVNHGSDALNELKRWQPDLLVLDIQLPDMSGMDILEIVQSEYPDVT
VIMITAHGSIDIAVDAMRSGAFDFLIKPFDAKRLSITVRNALKQRQLVNLVAKYESSLPKPHYMGFIGESLAMQTVYKTI
DCVASSKASAFIIGESGTGKEVCAHAIHNAGNRSDGPFVALNCASIPKDLIESEIFGHTKGAFTGAIANRDGAATRAHKG
TLFLDEICEMDLELQSKLLRFIQTGVFQRVGATKEEKVDVRFVSATNRMPWDEVKAGRFREDLFYRLHVIPIELPPLRMR
GKDILLLASSLLKEYNKEEGKTFKGFSPEAKQCLKSYPWPGNVRQLQNVIRQIVVLNDSETIEIDMLPIQLTSTSAIKSE
VAKPQAAVVKSQSLRGDSDASSFLDGIERAAFSGSEQYAKSANEKSDDIVPLWKTEKQTIENAIARCDGNVPKAAALLDI
SASTIYRKRQSWDELEKA
>Mature_498_residues
MDIASSFTTLLVEDSMSLGALYTEYLRTEGARVTHVNHGSDALNELKRWQPDLLVLDIQLPDMSGMDILEIVQSEYPDVT
VIMITAHGSIDIAVDAMRSGAFDFLIKPFDAKRLSITVRNALKQRQLVNLVAKYESSLPKPHYMGFIGESLAMQTVYKTI
DCVASSKASAFIIGESGTGKEVCAHAIHNAGNRSDGPFVALNCASIPKDLIESEIFGHTKGAFTGAIANRDGAATRAHKG
TLFLDEICEMDLELQSKLLRFIQTGVFQRVGATKEEKVDVRFVSATNRMPWDEVKAGRFREDLFYRLHVIPIELPPLRMR
GKDILLLASSLLKEYNKEEGKTFKGFSPEAKQCLKSYPWPGNVRQLQNVIRQIVVLNDSETIEIDMLPIQLTSTSAIKSE
VAKPQAAVVKSQSLRGDSDASSFLDGIERAAFSGSEQYAKSANEKSDDIVPLWKTEKQTIENAIARCDGNVPKAAALLDI
SASTIYRKRQSWDELEKA

Specific function: Acts negatively to control the expression of luminescence. At low cell density, luxO is phosphorylated, and together with sigma-54, causes repression of the luxCDABEGH operon. This repression could be indirect, luxO could activate a negative regulator of

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=478, Percent_Identity=38.0753138075314, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI1790437, Length=479, Percent_Identity=37.3695198329854, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI1788550, Length=484, Percent_Identity=36.7768595041322, Blast_Score=305, Evalue=5e-84,
Organism=Escherichia coli, GI1788905, Length=373, Percent_Identity=40.7506702412869, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1789233, Length=358, Percent_Identity=38.8268156424581, Blast_Score=235, Evalue=6e-63,
Organism=Escherichia coli, GI87082117, Length=311, Percent_Identity=42.1221864951768, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI87082152, Length=315, Percent_Identity=42.2222222222222, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI1789087, Length=308, Percent_Identity=40.5844155844156, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1786524, Length=376, Percent_Identity=35.1063829787234, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI87081872, Length=241, Percent_Identity=40.2489626556017, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1787583, Length=274, Percent_Identity=38.3211678832117, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI87081858, Length=328, Percent_Identity=29.2682926829268, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1789828, Length=231, Percent_Identity=32.034632034632, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1786838, Length=127, Percent_Identity=33.0708661417323, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1786784, Length=126, Percent_Identity=30.952380952381, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1788394, Length=122, Percent_Identity=29.5081967213115, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI87082012, Length=120, Percent_Identity=35, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1789809, Length=123, Percent_Identity=30.0813008130081, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 55083; Mature: 55083

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIASSFTTLLVEDSMSLGALYTEYLRTEGARVTHVNHGSDALNELKRWQPDLLVLDIQL
CCCHHHHHHHHEECCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEEC
PDMSGMDILEIVQSEYPDVTVIMITAHGSIDIAVDAMRSGAFDFLIKPFDAKRLSITVRN
CCCCCCHHHHHHHHCCCCEEEEEEEECCCEEEEEEHHHCCCCEEEECCCCCCEEEEHHHH
ALKQRQLVNLVAKYESSLPKPHYMGFIGESLAMQTVYKTIDCVASSKASAFIIGESGTGK
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCH
EVCAHAIHNAGNRSDGPFVALNCASIPKDLIESEIFGHTKGAFTGAIANRDGAATRAHKG
HHHHHHHHCCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCC
TLFLDEICEMDLELQSKLLRFIQTGVFQRVGATKEEKVDVRFVSATNRMPWDEVKAGRFR
CEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHCCHHH
EDLFYRLHVIPIELPPLRMRGKDILLLASSLLKEYNKEEGKTFKGFSPEAKQCLKSYPWP
HHHEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCHHCCCEECCCCHHHHHHHHCCCCC
GNVRQLQNVIRQIVVLNDSETIEIDMLPIQLTSTSAIKSEVAKPQAAVVKSQSLRGDSDA
CCHHHHHHHHHHHEEECCCCEEEEEEEEEEECCHHHHHHHHCCCHHHHHHHHCCCCCCHH
SSFLDGIERAAFSGSEQYAKSANEKSDDIVPLWKTEKQTIENAIARCDGNVPKAAALLDI
HHHHHHHHHHHCCCCHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCHHHEEEC
SASTIYRKRQSWDELEKA
HHHHHHHHHHCHHHHHCC
>Mature Secondary Structure
MDIASSFTTLLVEDSMSLGALYTEYLRTEGARVTHVNHGSDALNELKRWQPDLLVLDIQL
CCCHHHHHHHHEECCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEEC
PDMSGMDILEIVQSEYPDVTVIMITAHGSIDIAVDAMRSGAFDFLIKPFDAKRLSITVRN
CCCCCCHHHHHHHHCCCCEEEEEEEECCCEEEEEEHHHCCCCEEEECCCCCCEEEEHHHH
ALKQRQLVNLVAKYESSLPKPHYMGFIGESLAMQTVYKTIDCVASSKASAFIIGESGTGK
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCH
EVCAHAIHNAGNRSDGPFVALNCASIPKDLIESEIFGHTKGAFTGAIANRDGAATRAHKG
HHHHHHHHCCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCC
TLFLDEICEMDLELQSKLLRFIQTGVFQRVGATKEEKVDVRFVSATNRMPWDEVKAGRFR
CEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHCCHHH
EDLFYRLHVIPIELPPLRMRGKDILLLASSLLKEYNKEEGKTFKGFSPEAKQCLKSYPWP
HHHEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCHHCCCEECCCCHHHHHHHHCCCCC
GNVRQLQNVIRQIVVLNDSETIEIDMLPIQLTSTSAIKSEVAKPQAAVVKSQSLRGDSDA
CCHHHHHHHHHHHEEECCCCEEEEEEEEEEECCHHHHHHHHCCCHHHHHHHHCCCCCCHH
SSFLDGIERAAFSGSEQYAKSANEKSDDIVPLWKTEKQTIENAIARCDGNVPKAAALLDI
HHHHHHHHHHHCCCCHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCHHHEEEC
SASTIYRKRQSWDELEKA
HHHHHHHHHHCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8065259 [H]