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The map label for this gene is rmlA1 [C]

Identifier: 157961249

GI number: 157961249

Start: 1728315

End: 1729742

Strand: Reverse

Name: rmlA1 [C]

Synonym: Spea_1423

Alternate gene names: 157961249

Gene position: 1729742-1728315 (Counterclockwise)

Preceding gene: 157961250

Following gene: 157961248

Centisome position: 33.43

GC content: 44.19

Gene sequence:

>1428_bases
ATGCAAGCGATTATATTTGCAAACCGTGATGGTAAAGAACTGGCGCCACTCGATAGCTATTACTGCCCGGCTTTATTGCC
TATTAGTAATAAAAGCATTATCGAATACACATTAGAAGATCTTGCCGAGGCAGGAATAGAACGAGTCAAGCTAGTTGTTT
CTACACAAGTTAGCGAAATCAAAGTATTAGTCGGTAACGGTGAGCGTTGGGGATTAGAGGTCGAGTATTTTCTGAGTAAG
CAGCAGGAAGATATTTCAAAGATCATTCCAAGGCTCGCACTCGATAAAACCGAGTCGATTTTACTTGCCCGAGGAGACAT
GTTGAGGAGTCCTTGCATCAAAAATTTTGTGCAGTTTAGTCAGCAGATGAGCCAAAGCTTTGTCATTGCAAAGTTAGATG
AGCAAAATGCAGGCTTGATGATGCTACCCGCTGCGGTCTCCTACACAGAGGAGCTTAACTGGCCTCTGTGCAATGACGCC
GAGCATACAGGTAACAAACACAACATCGTTACTCAAGTACTGCATGGCGATTGCTTTATGCTAACCACCCTTGATGAATA
TATGCGAGCCAATCAAGCGATGGCGCTGAGAAAAGTGATAGGAGCCGAGCCCAAAGGGCGTTTCTGCAGCAGCGCCGATG
AAGCAAATGGCTTCTATGTTGGTGCCCAAACAAAAACCGGTCAACTGCGGATGCAAAATGCCTGGGGGATCATAGGCGAC
AACACTTGGATAGATGAAAGCGTTAACATGCAGCAAAGTGTTATTGTGGGTAAAAACTGCTTAATCGATGCTAGATCTAG
CCTCAAAAACTGCATTATTCTGGATGACTCTTATGTAGGCCAAGGCCTTAATGTGAGTAATGCCATTATCTGCAATAACA
TGCTACTCACGCCGAGTACTGGCGGTGCTATAAGACTAAACGATGACAGTATCATAGCAAAAAATCACAACAAGGCTGCT
AATCGCAAGGTCAAGCTGCTCGATAAACTCTGTGCTTTTTTGCTGTTTATCTTAGGGATTTTAATCTCCCCGGTTCTATT
AGTTGCCAGCCTTGCAAGCTCGAATAAAAAAACATTTATAAAAGATATCATTACCCTGAATAACACAAATTTTGCCTGCT
GGCGCTGGAACCTCAACTCACTCTTTCTTAGCAGATTGCCACAACTCTATCTTGTGGTGTTGGGGAGGTTGCACTTATTT
GGCCGTACTCCTTTTTTAAAGCAAGACCAGGGCTTTAACGATAACGAATTCGGACTATATGGGCCGTTGCAGCTCCGCCG
CGATGTCGTTCTTGAGGAGGAAAGACAGCTAGTCGAAACTGAGTTCATGCTGAATAAGCAAAAGAGTCAGCGCTGGAAAC
TCATCAAGCAATCATTTAGCTATTCACCACCAGAGCCACAAGCAACTTCCGAAGCGACAAAAAACTAG

Upstream 100 bases:

>100_bases
GATTATATCTACCAACAGTCATTTTCTGCCGATCTGTTACTGCTACTGAAAACAATCCCCGCAGTTATTTTTGCCCGTGG
CGCGTATTAAGGAGCGAGAT

Downstream 100 bases:

>100_bases
GAACGACCATACCTTAACAAGATCCATCCGATTCGATAGCACAGAGTCATTAGTAAGTGGAGAGTCATTATGAAAACAAT
TATCACCTACGGCACCTTCG

Product: nucleotidyl transferase

Products: dTDPglucose; Diphosphate [C]

Alternate protein names: Transferase Hexapeptide Repeat Protein; Nucleotidyl Transferase Domain-Containing Protein

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MQAIIFANRDGKELAPLDSYYCPALLPISNKSIIEYTLEDLAEAGIERVKLVVSTQVSEIKVLVGNGERWGLEVEYFLSK
QQEDISKIIPRLALDKTESILLARGDMLRSPCIKNFVQFSQQMSQSFVIAKLDEQNAGLMMLPAAVSYTEELNWPLCNDA
EHTGNKHNIVTQVLHGDCFMLTTLDEYMRANQAMALRKVIGAEPKGRFCSSADEANGFYVGAQTKTGQLRMQNAWGIIGD
NTWIDESVNMQQSVIVGKNCLIDARSSLKNCIILDDSYVGQGLNVSNAIICNNMLLTPSTGGAIRLNDDSIIAKNHNKAA
NRKVKLLDKLCAFLLFILGILISPVLLVASLASSNKKTFIKDIITLNNTNFACWRWNLNSLFLSRLPQLYLVVLGRLHLF
GRTPFLKQDQGFNDNEFGLYGPLQLRRDVVLEEERQLVETEFMLNKQKSQRWKLIKQSFSYSPPEPQATSEATKN

Sequences:

>Translated_475_residues
MQAIIFANRDGKELAPLDSYYCPALLPISNKSIIEYTLEDLAEAGIERVKLVVSTQVSEIKVLVGNGERWGLEVEYFLSK
QQEDISKIIPRLALDKTESILLARGDMLRSPCIKNFVQFSQQMSQSFVIAKLDEQNAGLMMLPAAVSYTEELNWPLCNDA
EHTGNKHNIVTQVLHGDCFMLTTLDEYMRANQAMALRKVIGAEPKGRFCSSADEANGFYVGAQTKTGQLRMQNAWGIIGD
NTWIDESVNMQQSVIVGKNCLIDARSSLKNCIILDDSYVGQGLNVSNAIICNNMLLTPSTGGAIRLNDDSIIAKNHNKAA
NRKVKLLDKLCAFLLFILGILISPVLLVASLASSNKKTFIKDIITLNNTNFACWRWNLNSLFLSRLPQLYLVVLGRLHLF
GRTPFLKQDQGFNDNEFGLYGPLQLRRDVVLEEERQLVETEFMLNKQKSQRWKLIKQSFSYSPPEPQATSEATKN
>Mature_475_residues
MQAIIFANRDGKELAPLDSYYCPALLPISNKSIIEYTLEDLAEAGIERVKLVVSTQVSEIKVLVGNGERWGLEVEYFLSK
QQEDISKIIPRLALDKTESILLARGDMLRSPCIKNFVQFSQQMSQSFVIAKLDEQNAGLMMLPAAVSYTEELNWPLCNDA
EHTGNKHNIVTQVLHGDCFMLTTLDEYMRANQAMALRKVIGAEPKGRFCSSADEANGFYVGAQTKTGQLRMQNAWGIIGD
NTWIDESVNMQQSVIVGKNCLIDARSSLKNCIILDDSYVGQGLNVSNAIICNNMLLTPSTGGAIRLNDDSIIAKNHNKAA
NRKVKLLDKLCAFLLFILGILISPVLLVASLASSNKKTFIKDIITLNNTNFACWRWNLNSLFLSRLPQLYLVVLGRLHLF
GRTPFLKQDQGFNDNEFGLYGPLQLRRDVVLEEERQLVETEFMLNKQKSQRWKLIKQSFSYSPPEPQATSEATKN

Specific function: Catalyzes The Formation Of Dtdp-Glucose, From Dttp And Glucose 1-Phosphate, As Well As Its Pyrophosphorolysis. [C]

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.24 [C]

Molecular weight: Translated: 53282; Mature: 53282

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAIIFANRDGKELAPLDSYYCPALLPISNKSIIEYTLEDLAEAGIERVKLVVSTQVSEI
CCEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHEEHHEEECCEEE
KVLVGNGERWGLEVEYFLSKQQEDISKIIPRLALDKTESILLARGDMLRSPCIKNFVQFS
EEEEECCCCCCEEEHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHH
QQMSQSFVIAKLDEQNAGLMMLPAAVSYTEELNWPLCNDAEHTGNKHNIVTQVLHGDCFM
HHHHCCEEEEEECCCCCCEEEECCHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHCCCEEE
LTTLDEYMRANQAMALRKVIGAEPKGRFCSSADEANGFYVGAQTKTGQLRMQNAWGIIGD
EEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCEEECC
NTWIDESVNMQQSVIVGKNCLIDARSSLKNCIILDDSYVGQGLNVSNAIICNNMLLTPST
CCCCCCCCCCHHHEEECCCCEEECHHCCCCEEEEECCCCCCCCCCCCEEEECCEEEECCC
GGAIRLNDDSIIAKNHNKAANRKVKLLDKLCAFLLFILGILISPVLLVASLASSNKKTFI
CCEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
KDIITLNNTNFACWRWNLNSLFLSRLPQLYLVVLGRLHLFGRTPFLKQDQGFNDNEFGLY
HHHHEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
GPLQLRRDVVLEEERQLVETEFMLNKQKSQRWKLIKQSFSYSPPEPQATSEATKN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQAIIFANRDGKELAPLDSYYCPALLPISNKSIIEYTLEDLAEAGIERVKLVVSTQVSEI
CCEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHEEHHEEECCEEE
KVLVGNGERWGLEVEYFLSKQQEDISKIIPRLALDKTESILLARGDMLRSPCIKNFVQFS
EEEEECCCCCCEEEHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHH
QQMSQSFVIAKLDEQNAGLMMLPAAVSYTEELNWPLCNDAEHTGNKHNIVTQVLHGDCFM
HHHHCCEEEEEECCCCCCEEEECCHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHCCCEEE
LTTLDEYMRANQAMALRKVIGAEPKGRFCSSADEANGFYVGAQTKTGQLRMQNAWGIIGD
EEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCEEECC
NTWIDESVNMQQSVIVGKNCLIDARSSLKNCIILDDSYVGQGLNVSNAIICNNMLLTPST
CCCCCCCCCCHHHEEECCCCEEECHHCCCCEEEEECCCCCCCCCCCCEEEECCEEEECCC
GGAIRLNDDSIIAKNHNKAANRKVKLLDKLCAFLLFILGILISPVLLVASLASSNKKTFI
CCEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
KDIITLNNTNFACWRWNLNSLFLSRLPQLYLVVLGRLHLFGRTPFLKQDQGFNDNEFGLY
HHHHEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
GPLQLRRDVVLEEERQLVETEFMLNKQKSQRWKLIKQSFSYSPPEPQATSEATKN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTTP; D-Glucose 1-phosphate; H+ [C]

Specific reaction: dTTP + D-Glucose 1-phosphate + H+ --> dTDPglucose + Diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA