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The map label for this gene is 157961243

Identifier: 157961243

GI number: 157961243

Start: 1720905

End: 1722413

Strand: Reverse

Name: 157961243

Synonym: Spea_1417

Alternate gene names: NA

Gene position: 1722413-1720905 (Counterclockwise)

Preceding gene: 157961247

Following gene: 157961220

Centisome position: 33.29

GC content: 44.0

Gene sequence:

>1509_bases
ATGCAAATTATCCATCATGGTGCTGTTAATGGAGTAACAGGCTCATGCCATCAGTTAGATATCAATCCACAATCCAGCAT
TCTAATCGATTGCGGCCTGTTTCAAGGAGCAGAAACAGCTGGTAGAAACGCCGTAGACCAACTTAAGATAGAATTTGACT
TAAGCCATATTACAGCGTTAATTGTTACCCACTGCCATATTGATCACGTTGGCAGGGTCCCATATCTACTTGCCGCAGGC
TTTAATGGCCCTATTTACGCAACAACGGCAACAGCAGCATTACTCCCGTTGGTCATCAAAGATGCGCTTAAGGTCGGCGT
CACCAGAAATCAGCGTTTAATCAGTTTATATCTAAAACGCTTACAGCAGATAATTAAGCCTATTGATTACCAAAAGTGGT
TTAATTTAGAGGTGAATAATAGCGACTGCGGCTACCAACACGCCAAAGCTAGGTTTAACGTAGCAGGACATATTCTAGGC
TCGGCCTATGTTGAAATAGAACTAAGTGACGCATCGCTAACTCACAACTGTACTCACAACTATACTCGCAATAATACTCA
TAAAAGCACTCACCAGAACACTGGTATTCACAGCGACAACAGTCATGACTGTGATGCAGACAAGAAAAAAGACAGCCATA
TGGTTGTATTTTCTGGTGACTTAGGCGCAACTAACACCCCGCTATTGCCAAGCCCTAAAAGCCCATTGCGCGCCGACACA
TTAGTAATAGAGTCGACCTATGGTGATAAAAATCACCATAATCGTGAGCACAGAGCCGAAAGCTTACGCCAAGTGATAGA
GAGAGCAGTAAACGACAACGGTGTTGTTCTTATTCCGGCCTTTAGCATTGGCCGAACCCAAGAGTTACTGTATGAATTAG
AGCAAATTATCTTTAGCCAACAACAAAACCCGATGTGGCAGAGTATAGACATCATTGTCGACTCCCCTATGGCAGCCAAT
TTTACCGAGCAATATATAGATTATAAACACTTGTGGGACACAGAAGCCAAAGCAGTACTAGCGGAGCAACGCCATCCGCT
CGACTTTAGTCAGCTTTACACCATAGATAGCCACCAAGATCACCTAGCGGTAATCAACTACCTAGCCAGCAGACAAAAGC
CAACTGTTGTAATTGCAGCAAGCGGGATGTGCACAGGCGGGCGAATCGTCAACTATCTACAGCGATTTCTATCTGAACCG
ACTGCAGATGTGCTATTTGTGGGATACCAAGCAACTGGAACTATCGGACGAGATATTCAAGAATACGGACCACGCGGAGG
TTATGTATATATTGAAGGGGATAAAATCGATATCAAAGCAGGAATTCACACCATCAGTGGCTATTCCGCCCATGCAGATC
AACAAGATCTGATTGATTTTGTCGAAGGCATCAAACATAAACCAAAACATATCCGCATCGTTCACGGCGACACCGAGGCA
AAACAAACGTTAGCGGATAAATACCGCCAACTCTTACCTAACACAGAGATTGAAATAGGAACAAGCTAG

Upstream 100 bases:

>100_bases
TAAGCTCATATAGAGTCTAGCAGGAGCATAGCGACGCTCTAGCCGTGACGGAGCGACGTTCTAGTCACTACAATTAATAA
CAACGATAAGGAAGTCGTAC

Downstream 100 bases:

>100_bases
AGCTATAAAGCTATTCACCACAGAGGGCACGGAGCGATGCGCTGCGCTACACGGAGAAAGACCGTTGGGAATTAAATTAA
ACACATGATTCGAGAACACT

Product: RNA-metabolising metallo-beta-lactamase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MQIIHHGAVNGVTGSCHQLDINPQSSILIDCGLFQGAETAGRNAVDQLKIEFDLSHITALIVTHCHIDHVGRVPYLLAAG
FNGPIYATTATAALLPLVIKDALKVGVTRNQRLISLYLKRLQQIIKPIDYQKWFNLEVNNSDCGYQHAKARFNVAGHILG
SAYVEIELSDASLTHNCTHNYTRNNTHKSTHQNTGIHSDNSHDCDADKKKDSHMVVFSGDLGATNTPLLPSPKSPLRADT
LVIESTYGDKNHHNREHRAESLRQVIERAVNDNGVVLIPAFSIGRTQELLYELEQIIFSQQQNPMWQSIDIIVDSPMAAN
FTEQYIDYKHLWDTEAKAVLAEQRHPLDFSQLYTIDSHQDHLAVINYLASRQKPTVVIAASGMCTGGRIVNYLQRFLSEP
TADVLFVGYQATGTIGRDIQEYGPRGGYVYIEGDKIDIKAGIHTISGYSAHADQQDLIDFVEGIKHKPKHIRIVHGDTEA
KQTLADKYRQLLPNTEIEIGTS

Sequences:

>Translated_502_residues
MQIIHHGAVNGVTGSCHQLDINPQSSILIDCGLFQGAETAGRNAVDQLKIEFDLSHITALIVTHCHIDHVGRVPYLLAAG
FNGPIYATTATAALLPLVIKDALKVGVTRNQRLISLYLKRLQQIIKPIDYQKWFNLEVNNSDCGYQHAKARFNVAGHILG
SAYVEIELSDASLTHNCTHNYTRNNTHKSTHQNTGIHSDNSHDCDADKKKDSHMVVFSGDLGATNTPLLPSPKSPLRADT
LVIESTYGDKNHHNREHRAESLRQVIERAVNDNGVVLIPAFSIGRTQELLYELEQIIFSQQQNPMWQSIDIIVDSPMAAN
FTEQYIDYKHLWDTEAKAVLAEQRHPLDFSQLYTIDSHQDHLAVINYLASRQKPTVVIAASGMCTGGRIVNYLQRFLSEP
TADVLFVGYQATGTIGRDIQEYGPRGGYVYIEGDKIDIKAGIHTISGYSAHADQQDLIDFVEGIKHKPKHIRIVHGDTEA
KQTLADKYRQLLPNTEIEIGTS
>Mature_502_residues
MQIIHHGAVNGVTGSCHQLDINPQSSILIDCGLFQGAETAGRNAVDQLKIEFDLSHITALIVTHCHIDHVGRVPYLLAAG
FNGPIYATTATAALLPLVIKDALKVGVTRNQRLISLYLKRLQQIIKPIDYQKWFNLEVNNSDCGYQHAKARFNVAGHILG
SAYVEIELSDASLTHNCTHNYTRNNTHKSTHQNTGIHSDNSHDCDADKKKDSHMVVFSGDLGATNTPLLPSPKSPLRADT
LVIESTYGDKNHHNREHRAESLRQVIERAVNDNGVVLIPAFSIGRTQELLYELEQIIFSQQQNPMWQSIDIIVDSPMAAN
FTEQYIDYKHLWDTEAKAVLAEQRHPLDFSQLYTIDSHQDHLAVINYLASRQKPTVVIAASGMCTGGRIVNYLQRFLSEP
TADVLFVGYQATGTIGRDIQEYGPRGGYVYIEGDKIDIKAGIHTISGYSAHADQQDLIDFVEGIKHKPKHIRIVHGDTEA
KQTLADKYRQLLPNTEIEIGTS

Specific function: Has endoribonuclease activity towards 23S and 16S rRNA (in vitro) [H]

COG id: COG1236

COG function: function code J; Predicted exonuclease of the beta-lactamase fold involved in RNA processing

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family [H]

Homologues:

Organism=Homo sapiens, GI7706427, Length=498, Percent_Identity=26.3052208835341, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI33300633, Length=516, Percent_Identity=23.062015503876, Blast_Score=119, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI32566029, Length=483, Percent_Identity=26.0869565217391, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI32564696, Length=507, Percent_Identity=23.6686390532544, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323307, Length=505, Percent_Identity=24.5544554455446, Blast_Score=119, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24648013, Length=485, Percent_Identity=25.5670103092783, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI21358523, Length=504, Percent_Identity=24.4047619047619, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022712
- InterPro:   IPR001279
- InterPro:   IPR011108 [H]

Pfam domain/function: PF10996 Beta-Casp; PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 55890; Mature: 55890

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIIHHGAVNGVTGSCHQLDINPQSSILIDCGLFQGAETAGRNAVDQLKIEFDLSHITAL
CCEEECCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCCCCCCCHHHEEEEECHHHHHHH
IVTHCHIDHVGRVPYLLAAGFNGPIYATTATAALLPLVIKDALKVGVTRNQRLISLYLKR
HHHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LQQIIKPIDYQKWFNLEVNNSDCGYQHAKARFNVAGHILGSAYVEIELSDASLTHNCTHN
HHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHEEEECCEEEEEEECCCCEECCCCCC
YTRNNTHKSTHQNTGIHSDNSHDCDADKKKDSHMVVFSGDLGATNTPLLPSPKSPLRADT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCE
LVIESTYGDKNHHNREHRAESLRQVIERAVNDNGVVLIPAFSIGRTQELLYELEQIIFSQ
EEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHC
QQNPMWQSIDIIVDSPMAANFTEQYIDYKHLWDTEAKAVLAEQRHPLDFSQLYTIDSHQD
CCCCCCEEEEEEEECCCCCCHHHHHCCHHHHCCCHHHHHHHHCCCCCCHHHEEEECCCCC
HLAVINYLASRQKPTVVIAASGMCTGGRIVNYLQRFLSEPTADVLFVGYQATGTIGRDIQ
HHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCHHCCHH
EYGPRGGYVYIEGDKIDIKAGIHTISGYSAHADQQDLIDFVEGIKHKPKHIRIVHGDTEA
HHCCCCCEEEEECCEEEEEECEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHH
KQTLADKYRQLLPNTEIEIGTS
HHHHHHHHHHHCCCCCEEECCC
>Mature Secondary Structure
MQIIHHGAVNGVTGSCHQLDINPQSSILIDCGLFQGAETAGRNAVDQLKIEFDLSHITAL
CCEEECCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCCCCCCCHHHEEEEECHHHHHHH
IVTHCHIDHVGRVPYLLAAGFNGPIYATTATAALLPLVIKDALKVGVTRNQRLISLYLKR
HHHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LQQIIKPIDYQKWFNLEVNNSDCGYQHAKARFNVAGHILGSAYVEIELSDASLTHNCTHN
HHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHEEEECCEEEEEEECCCCEECCCCCC
YTRNNTHKSTHQNTGIHSDNSHDCDADKKKDSHMVVFSGDLGATNTPLLPSPKSPLRADT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCE
LVIESTYGDKNHHNREHRAESLRQVIERAVNDNGVVLIPAFSIGRTQELLYELEQIIFSQ
EEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHC
QQNPMWQSIDIIVDSPMAANFTEQYIDYKHLWDTEAKAVLAEQRHPLDFSQLYTIDSHQD
CCCCCCEEEEEEEECCCCCCHHHHHCCHHHHCCCHHHHHHHHCCCCCCHHHEEEECCCCC
HLAVINYLASRQKPTVVIAASGMCTGGRIVNYLQRFLSEPTADVLFVGYQATGTIGRDIQ
HHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCHHCCHH
EYGPRGGYVYIEGDKIDIKAGIHTISGYSAHADQQDLIDFVEGIKHKPKHIRIVHGDTEA
HHCCCCCEEEEECCEEEEEECEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHH
KQTLADKYRQLLPNTEIEIGTS
HHHHHHHHHHHCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA