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The map label for this gene is hisS [H]

Identifier: 157961134

GI number: 157961134

Start: 1590407

End: 1591681

Strand: Direct

Name: hisS [H]

Synonym: Spea_1306

Alternate gene names: 157961134

Gene position: 1590407-1591681 (Clockwise)

Preceding gene: 157961133

Following gene: 157961135

Centisome position: 30.73

GC content: 46.9

Gene sequence:

>1275_bases
GTGGCAAAACAGATCCAAGCGATTCGCGGAATGAATGACATTCTGCCTACCCAAAGTCCTTTATGGCAAAAACTTGAAAC
CGTATTACGTGACACTGTTGGGTCATATGGTTACAGCGAAATTCGTACTCCTATCGTTGAAAGTACAGATCTCTTTAAGC
GCTCTATCGGTGAAGTAACTGATATCGTTGAAAAAGAGATGTATACCTTTGAAGATCGAAACGGTGACAGCTTAACCCTA
CGTCCAGAAGGCACTGCCTCTACGGTACGTGCGGGTAACGAACACGGTTTGCTATACAACCAAGAGCAGCGTCTTTGGTA
TATGGGACCAATGTTCCGCCACGAACGCCCACAAAAAGGCCGTTACCGTCAATTCCACCAATTTGGTGTTGAAGTTTACG
GCATTCCTACTGCCGATATCGACGCTGAAGTCTTGATGCTATCAGCCAAGCTTTGGGAAAAACTCGGTATTACTGAGCAT
GTGACCCTAGAGCTAAATACTTTAGGTGATACTGAAGAGCGCGCCGCATACCGTGATGCCTTGATTGCTTTCTTAGAGCA
ACACAAAGAGGTGTTAGACGAAGATAGCCAGCGCCGCATGTACAGTAACCCACTGCGCGTTTTGGACTCTAAGAATGCCG
ATGTACAGGCACTTCTAGCCGATGCGCCAGTGCTAATGGATTATTTCGGTGAAGACACGCGTTCACATTTTTCACATTTA
TGTGAACTCTTAGAGGCTGTCGGCATCCAATACACAATCAATCCTCGCCTAGTGCGCGGTTTAGATTATTATAATCGCAC
GGTTTTTGAGTGGGTGACATCGAGCCTAGGTTCGCAAGGTACAGTATTGGCCGGTGGTCGTTACGATGGTCTTGTTGGCC
AGCTAGGTGGTAAGCCAACACCGGCAGTCGGTTTTGCAATGGGCCTTGAGCGCATCGTATTACTACTGCAAACACTTGAG
CTCGACAAAGATATCGGTCCAGCTGTTGACGTATACGTGACAGCGATGGGTGATAACTGCAAAGTTGAAGCGATTAAGAT
TGCGCAAGAACTAAGAGCTAGCTTGCCAACTGCTAAAGTGATGAGCCATTGTGGTGGCGGTAACTTTAAGAAGCAGATGA
AGCGAGCCGATAAGAGCGGTGCCTGCGTTGCACTGGTGATTGGTGAAGATGAACTTGCCAATAATCAAGTTGCAGTGAAA
CACCTTCGCGAAGATAAAGCACAAGAATTAGTTGCCCGTGATGCGTTGGCGACATATATTGCAGAGCTGATTTAA

Upstream 100 bases:

>100_bases
TTTTTTATGGCTGAATGCACCTGTTGTACGTTTAGTCTCGAAACGCCTATAATGCGAGGCGTTTTTTAATTTTTATATAG
CAAACTTGACGAGTCGAATA

Downstream 100 bases:

>100_bases
AAGGGGAAGAGTAAGTGGAAATTTATAGCACAGAAGATCAACAAGTAGATGCTATCAAACAGTTCTGGAAAGATTACGGT
ACCTCAATTGTTGTTGGCGC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MAKQIQAIRGMNDILPTQSPLWQKLETVLRDTVGSYGYSEIRTPIVESTDLFKRSIGEVTDIVEKEMYTFEDRNGDSLTL
RPEGTASTVRAGNEHGLLYNQEQRLWYMGPMFRHERPQKGRYRQFHQFGVEVYGIPTADIDAEVLMLSAKLWEKLGITEH
VTLELNTLGDTEERAAYRDALIAFLEQHKEVLDEDSQRRMYSNPLRVLDSKNADVQALLADAPVLMDYFGEDTRSHFSHL
CELLEAVGIQYTINPRLVRGLDYYNRTVFEWVTSSLGSQGTVLAGGRYDGLVGQLGGKPTPAVGFAMGLERIVLLLQTLE
LDKDIGPAVDVYVTAMGDNCKVEAIKIAQELRASLPTAKVMSHCGGGNFKKQMKRADKSGACVALVIGEDELANNQVAVK
HLREDKAQELVARDALATYIAELI

Sequences:

>Translated_424_residues
MAKQIQAIRGMNDILPTQSPLWQKLETVLRDTVGSYGYSEIRTPIVESTDLFKRSIGEVTDIVEKEMYTFEDRNGDSLTL
RPEGTASTVRAGNEHGLLYNQEQRLWYMGPMFRHERPQKGRYRQFHQFGVEVYGIPTADIDAEVLMLSAKLWEKLGITEH
VTLELNTLGDTEERAAYRDALIAFLEQHKEVLDEDSQRRMYSNPLRVLDSKNADVQALLADAPVLMDYFGEDTRSHFSHL
CELLEAVGIQYTINPRLVRGLDYYNRTVFEWVTSSLGSQGTVLAGGRYDGLVGQLGGKPTPAVGFAMGLERIVLLLQTLE
LDKDIGPAVDVYVTAMGDNCKVEAIKIAQELRASLPTAKVMSHCGGGNFKKQMKRADKSGACVALVIGEDELANNQVAVK
HLREDKAQELVARDALATYIAELI
>Mature_423_residues
AKQIQAIRGMNDILPTQSPLWQKLETVLRDTVGSYGYSEIRTPIVESTDLFKRSIGEVTDIVEKEMYTFEDRNGDSLTLR
PEGTASTVRAGNEHGLLYNQEQRLWYMGPMFRHERPQKGRYRQFHQFGVEVYGIPTADIDAEVLMLSAKLWEKLGITEHV
TLELNTLGDTEERAAYRDALIAFLEQHKEVLDEDSQRRMYSNPLRVLDSKNADVQALLADAPVLMDYFGEDTRSHFSHLC
ELLEAVGIQYTINPRLVRGLDYYNRTVFEWVTSSLGSQGTVLAGGRYDGLVGQLGGKPTPAVGFAMGLERIVLLLQTLEL
DKDIGPAVDVYVTAMGDNCKVEAIKIAQELRASLPTAKVMSHCGGGNFKKQMKRADKSGACVALVIGEDELANNQVAVKH
LREDKAQELVARDALATYIAELI

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=458, Percent_Identity=24.8908296943231, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1788861, Length=424, Percent_Identity=67.9245283018868, Blast_Score=603, Evalue=1e-174,
Organism=Saccharomyces cerevisiae, GI6325290, Length=462, Percent_Identity=24.4588744588745, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 47375; Mature: 47243

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQIQAIRGMNDILPTQSPLWQKLETVLRDTVGSYGYSEIRTPIVESTDLFKRSIGEVT
CCCHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHH
DIVEKEMYTFEDRNGDSLTLRPEGTASTVRAGNEHGLLYNQEQRLWYMGPMFRHERPQKG
HHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCCCCCEECCCCCEEEECCHHHCCCCCCC
RYRQFHQFGVEVYGIPTADIDAEVLMLSAKLWEKLGITEHVTLELNTLGDTEERAAYRDA
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHH
LIAFLEQHKEVLDEDSQRRMYSNPLRVLDSKNADVQALLADAPVLMDYFGEDTRSHFSHL
HHHHHHHHHHHHCCHHHHHHHHCCHHHHCCCCCCHHHHHHCCHHHHHHHCCHHHHHHHHH
CELLEAVGIQYTINPRLVRGLDYYNRTVFEWVTSSLGSQGTVLAGGRYDGLVGQLGGKPT
HHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHCCCCCC
PAVGFAMGLERIVLLLQTLELDKDIGPAVDVYVTAMGDNCKVEAIKIAQELRASLPTAKV
CHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCHHHH
MSHCGGGNFKKQMKRADKSGACVALVIGEDELANNQVAVKHLREDKAQELVARDALATYI
HHHCCCCCHHHHHHHCCCCCCEEEEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AELI
HHHC
>Mature Secondary Structure 
AKQIQAIRGMNDILPTQSPLWQKLETVLRDTVGSYGYSEIRTPIVESTDLFKRSIGEVT
CCHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHH
DIVEKEMYTFEDRNGDSLTLRPEGTASTVRAGNEHGLLYNQEQRLWYMGPMFRHERPQKG
HHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCCCCCEECCCCCEEEECCHHHCCCCCCC
RYRQFHQFGVEVYGIPTADIDAEVLMLSAKLWEKLGITEHVTLELNTLGDTEERAAYRDA
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHH
LIAFLEQHKEVLDEDSQRRMYSNPLRVLDSKNADVQALLADAPVLMDYFGEDTRSHFSHL
HHHHHHHHHHHHCCHHHHHHHHCCHHHHCCCCCCHHHHHHCCHHHHHHHCCHHHHHHHHH
CELLEAVGIQYTINPRLVRGLDYYNRTVFEWVTSSLGSQGTVLAGGRYDGLVGQLGGKPT
HHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHCCCCCC
PAVGFAMGLERIVLLLQTLELDKDIGPAVDVYVTAMGDNCKVEAIKIAQELRASLPTAKV
CHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCHHHH
MSHCGGGNFKKQMKRADKSGACVALVIGEDELANNQVAVKHLREDKAQELVARDALATYI
HHHCCCCCHHHHHHHCCCCCCEEEEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AELI
HHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA