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The map label for this gene is alkR [H]

Identifier: 157961077

GI number: 157961077

Start: 1522829

End: 1523641

Strand: Reverse

Name: alkR [H]

Synonym: Spea_1249

Alternate gene names: 157961077

Gene position: 1523641-1522829 (Counterclockwise)

Preceding gene: 157961080

Following gene: 157961072

Centisome position: 29.44

GC content: 41.57

Gene sequence:

>813_bases
ATGGATACCTTATCCGCATTAATATCTCATGCAGAACCCAAAGCGAACTTATTCTTCTCAGGTAATTTATGTGGAAGCTC
TTCAAGTACTGAGCACCCTGCTGGTGGAATATTGCATTTAGTTAGAACCGGTGAGATGCTTCTATTTGAAGAGCATGGCT
TAACGCATAGAATCACTGAACCAACACTCATTATTTATCCCAAAGGGAAAGCGCATCGTCTCCAACCGCAAACCTTAGAA
GGTTGTGATGTTGTATGTGCCAACTTAACCTTTAAAGATTCGCCACTATACCGAGCTCTACCCGATGCTATAGTTCTCCC
CCTTAGCGAATTAGATAGTTTATCAGACACAATCAACATGTTATTTGTTGAGGCTTTTACATCGCGTTTTGCTCAAGTTG
CCATCATCTCAACTTTACTTGATCTGCTGCTGTTATTGGTACTTCGTCATTTAGTTGAAAACAGAATATGTCAAGAAGGT
GTTTTATCAGCTTTATCAGATCCCAAGCTCTCAAAAGCCATCGAAATAATCCACAAACAAATAGATTACCCATGGACCAT
TGATGAGTTAGCAAATAAAGTTGGGATGTCACGTGCTAGGTTTGCAGCATTATTTAATCAAACCATTGGACAACCACCTC
TCAGCTACGTCACAGAGGCTCGGCTGCTGTTAGCTAAGAAGTTGCTGCTGAAGGGAGAACCAATCAAATCAGTAAGTTTA
GAAGTAGGGTACAGCGGAAGCGTTGCATTTTCTCGCGCATTCCAACGTCAATTCGGTGAAACTCCACGAGCCTGGTATCT
GGAACAAACTTAA

Upstream 100 bases:

>100_bases
CTGAGCTCCTTTGATTAAGTGAATTTACGATATTGATATTGACACTATCAGTAATACGATTTATCACTATTAATCTCGAT
ATACATACCCAGAGTCTCAA

Downstream 100 bases:

>100_bases
ATAAGCATGTCTGTCCACTTCGTTTTCAGCTTTATCGTTTAAGCGACATAAAATATTTTTAAATTATTTAAAAGTGACTA
GACTTTGATGTAACACCAAA

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MDTLSALISHAEPKANLFFSGNLCGSSSSTEHPAGGILHLVRTGEMLLFEEHGLTHRITEPTLIIYPKGKAHRLQPQTLE
GCDVVCANLTFKDSPLYRALPDAIVLPLSELDSLSDTINMLFVEAFTSRFAQVAIISTLLDLLLLLVLRHLVENRICQEG
VLSALSDPKLSKAIEIIHKQIDYPWTIDELANKVGMSRARFAALFNQTIGQPPLSYVTEARLLLAKKLLLKGEPIKSVSL
EVGYSGSVAFSRAFQRQFGETPRAWYLEQT

Sequences:

>Translated_270_residues
MDTLSALISHAEPKANLFFSGNLCGSSSSTEHPAGGILHLVRTGEMLLFEEHGLTHRITEPTLIIYPKGKAHRLQPQTLE
GCDVVCANLTFKDSPLYRALPDAIVLPLSELDSLSDTINMLFVEAFTSRFAQVAIISTLLDLLLLLVLRHLVENRICQEG
VLSALSDPKLSKAIEIIHKQIDYPWTIDELANKVGMSRARFAALFNQTIGQPPLSYVTEARLLLAKKLLLKGEPIKSVSL
EVGYSGSVAFSRAFQRQFGETPRAWYLEQT
>Mature_270_residues
MDTLSALISHAEPKANLFFSGNLCGSSSSTEHPAGGILHLVRTGEMLLFEEHGLTHRITEPTLIIYPKGKAHRLQPQTLE
GCDVVCANLTFKDSPLYRALPDAIVLPLSELDSLSDTINMLFVEAFTSRFAQVAIISTLLDLLLLLVLRHLVENRICQEG
VLSALSDPKLSKAIEIIHKQIDYPWTIDELANKVGMSRARFAALFNQTIGQPPLSYVTEARLLLAKKLLLKGEPIKSVSL
EVGYSGSVAFSRAFQRQFGETPRAWYLEQT

Specific function: This protein activates the expression of the alkane 1- monooxygenase AlkM [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786496, Length=282, Percent_Identity=27.6595744680851, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 29871; Mature: 29871

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTLSALISHAEPKANLFFSGNLCGSSSSTEHPAGGILHLVRTGEMLLFEEHGLTHRITE
CCHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCEEECCC
PTLIIYPKGKAHRLQPQTLEGCDVVCANLTFKDSPLYRALPDAIVLPLSELDSLSDTINM
CEEEEEECCCCCCCCCCCCCCCCEEEECCEECCCCHHHHCCHHHCCCHHHHHHHHHHHHH
LFVEAFTSRFAQVAIISTLLDLLLLLVLRHLVENRICQEGVLSALSDPKLSKAIEIIHKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
IDYPWTIDELANKVGMSRARFAALFNQTIGQPPLSYVTEARLLLAKKLLLKGEPIKSVSL
CCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEE
EVGYSGSVAFSRAFQRQFGETPRAWYLEQT
EECCCCCHHHHHHHHHHHCCCCCCEEECCC
>Mature Secondary Structure
MDTLSALISHAEPKANLFFSGNLCGSSSSTEHPAGGILHLVRTGEMLLFEEHGLTHRITE
CCHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCEEECCC
PTLIIYPKGKAHRLQPQTLEGCDVVCANLTFKDSPLYRALPDAIVLPLSELDSLSDTINM
CEEEEEECCCCCCCCCCCCCCCCEEEECCEECCCCHHHHCCHHHCCCHHHHHHHHHHHHH
LFVEAFTSRFAQVAIISTLLDLLLLLVLRHLVENRICQEGVLSALSDPKLSKAIEIIHKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
IDYPWTIDELANKVGMSRARFAALFNQTIGQPPLSYVTEARLLLAKKLLLKGEPIKSVSL
CCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEE
EVGYSGSVAFSRAFQRQFGETPRAWYLEQT
EECCCCCHHHHHHHHHHHCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9546151 [H]