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The map label for this gene is nfo [H]

Identifier: 157960898

GI number: 157960898

Start: 1294467

End: 1295321

Strand: Reverse

Name: nfo [H]

Synonym: Spea_1070

Alternate gene names: 157960898

Gene position: 1295321-1294467 (Counterclockwise)

Preceding gene: 157960900

Following gene: 157960893

Centisome position: 25.03

GC content: 46.78

Gene sequence:

>855_bases
ATGCAAGCTACTCACCTTATTGGAGCACATGTTAGCGCAGCTGGAGGCATAGCCAATGCCCCCCTTAACGCAGCAGCCAT
TGGCGCAAATGCTTTTGCCCTATTCACCAAGAACCAACGTCGATGGCAAGCAGCCCCATTAGATCCTAAGCAGGTAGCAC
TATTTCAGCAGCACTGCGTCGAAGCTGACATTGCATTTGAGGCGATTTTGCCCCATGACAGCTACCTCATAAATCTTGGC
CACCCCGATCCTGAGTTACTCAATAAGTCACGAGAAGCCTTTTATGATGAAATGCAGCGCTGTGAACAGATAGGATTAAA
GCTACTCAATTTTCATCCGGGTAGCCATCTTCGAAAAATGCCTATCGATGACTGCTTAACCAATATTAGCGAGTCGATTA
ATTTAGCCTTGGAACGTACATCTAATGTCACTGCAGTAATCGAAAATACCGCAGGTCAGGGCTCAAACCTTGGCCACAGC
TTCGAGCAACTAGCACAGATTATCGATGGAGTTGAGGATAAAACTAGAGTTGGTATCTGTATCGATACCTGCCACCTATT
TGCTGCAGGCTACGATATTCGAACTAAAGCCACCTGCGAGCAGACTTTTGAACTATTCGATAAAATTGTCGGCAACCGAT
ACCTAAAGGCCATGCATCTGAATGACAGCAAGACGCCGCTCAACAGTCGGGTCGACCGTCACGAGTCTTTAGGTCAAGGT
GAAATCGGCCAAGAGGCATTTGCCAGCTTAATGAAGTTAGATGCCGTAAAGGGGATCCCGCTAATTTTAGAGACGGTACG
AGCAGAGATCTGGCAAGAGGAGATTAATTGGTTACGCTACCTTGCAAAACAGTAA

Upstream 100 bases:

>100_bases
GCTATTGGACTGACTAAAAATAAATATGCTCAGTACAGGGTTTATCTTGCATGGCATAATAGCCAAAGAACAAACCTAGG
CTCAACAAGGATACATAAAG

Downstream 100 bases:

>100_bases
GGCAGCGCTGAAGGACTAGATCAAACACTTATAGATCACAGCGACCACTTTTGTTGGCTCACCCAAGTCAGATTGCAACC
ATACGATATGAGTGCCTTTA

Product: apurinic endonuclease Apn1

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MQATHLIGAHVSAAGGIANAPLNAAAIGANAFALFTKNQRRWQAAPLDPKQVALFQQHCVEADIAFEAILPHDSYLINLG
HPDPELLNKSREAFYDEMQRCEQIGLKLLNFHPGSHLRKMPIDDCLTNISESINLALERTSNVTAVIENTAGQGSNLGHS
FEQLAQIIDGVEDKTRVGICIDTCHLFAAGYDIRTKATCEQTFELFDKIVGNRYLKAMHLNDSKTPLNSRVDRHESLGQG
EIGQEAFASLMKLDAVKGIPLILETVRAEIWQEEINWLRYLAKQ

Sequences:

>Translated_284_residues
MQATHLIGAHVSAAGGIANAPLNAAAIGANAFALFTKNQRRWQAAPLDPKQVALFQQHCVEADIAFEAILPHDSYLINLG
HPDPELLNKSREAFYDEMQRCEQIGLKLLNFHPGSHLRKMPIDDCLTNISESINLALERTSNVTAVIENTAGQGSNLGHS
FEQLAQIIDGVEDKTRVGICIDTCHLFAAGYDIRTKATCEQTFELFDKIVGNRYLKAMHLNDSKTPLNSRVDRHESLGQG
EIGQEAFASLMKLDAVKGIPLILETVRAEIWQEEINWLRYLAKQ
>Mature_284_residues
MQATHLIGAHVSAAGGIANAPLNAAAIGANAFALFTKNQRRWQAAPLDPKQVALFQQHCVEADIAFEAILPHDSYLINLG
HPDPELLNKSREAFYDEMQRCEQIGLKLLNFHPGSHLRKMPIDDCLTNISESINLALERTSNVTAVIENTAGQGSNLGHS
FEQLAQIIDGVEDKTRVGICIDTCHLFAAGYDIRTKATCEQTFELFDKIVGNRYLKAMHLNDSKTPLNSRVDRHESLGQG
EIGQEAFASLMKLDAVKGIPLILETVRAEIWQEEINWLRYLAKQ

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=273, Percent_Identity=65.2014652014652, Blast_Score=375, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17531193, Length=263, Percent_Identity=47.5285171102662, Blast_Score=272, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6322735, Length=282, Percent_Identity=41.1347517730496, Blast_Score=230, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 31483; Mature: 31483

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQATHLIGAHVSAAGGIANAPLNAAAIGANAFALFTKNQRRWQAAPLDPKQVALFQQHCV
CCCCHHHHHHHHHCCCCCCCCCCHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
EADIAFEAILPHDSYLINLGHPDPELLNKSREAFYDEMQRCEQIGLKLLNFHPGSHLRKM
HHHHHHHEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCC
PIDDCLTNISESINLALERTSNVTAVIENTAGQGSNLGHSFEQLAQIIDGVEDKTRVGIC
CHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEE
IDTCHLFAAGYDIRTKATCEQTFELFDKIVGNRYLKAMHLNDSKTPLNSRVDRHESLGQG
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCHHHHHHHHHCCCCC
EIGQEAFASLMKLDAVKGIPLILETVRAEIWQEEINWLRYLAKQ
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQATHLIGAHVSAAGGIANAPLNAAAIGANAFALFTKNQRRWQAAPLDPKQVALFQQHCV
CCCCHHHHHHHHHCCCCCCCCCCHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
EADIAFEAILPHDSYLINLGHPDPELLNKSREAFYDEMQRCEQIGLKLLNFHPGSHLRKM
HHHHHHHEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCC
PIDDCLTNISESINLALERTSNVTAVIENTAGQGSNLGHSFEQLAQIIDGVEDKTRVGIC
CHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEE
IDTCHLFAAGYDIRTKATCEQTFELFDKIVGNRYLKAMHLNDSKTPLNSRVDRHESLGQG
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCHHHHHHHHHCCCCC
EIGQEAFASLMKLDAVKGIPLILETVRAEIWQEEINWLRYLAKQ
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA