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The map label for this gene is gltT [H]

Identifier: 157960603

GI number: 157960603

Start: 960429

End: 961727

Strand: Direct

Name: gltT [H]

Synonym: Spea_0775

Alternate gene names: 157960603

Gene position: 960429-961727 (Clockwise)

Preceding gene: 157960602

Following gene: 157960605

Centisome position: 18.56

GC content: 46.11

Gene sequence:

>1299_bases
ATGTCGAATCAACAACCTTCACTTCTGAAAAAAGTGTGGTCTAGCTGGATGTCTGTGCCACTTTGGCTACAGATCATGAT
TGGTATGCTGTTAGGTATTTTAACCGGGGTTAGCTTGGGTGAACAGGCGACCTACTTGAAGCCAATTGGTACTCTATTTG
TTAATACTATCAAAATGTTGATTGTACCATTAGTGTTCTGCTCCCTAATTGTCGGCGTAACTTCTATGCAAGATACCGCC
AAGATGGGACGCATTGGTTTCAAGTCGTTCGCTTTCTACTTAGGCACAACCTCTATCGCAATCACGCTAGGTTTAGCCGT
TGGCCACATTATGCAGCCGGGCACGGGCCTTGACATGGTAGCTCATGAAACAGCTGAAGTGGTCAAGCAAGCGCCGTCAA
TCATGCAGACCTTGATTGATATCGTGCCAACTAACCCGATTGCAGCGCTTGCTAGTGGCCAGATTTTACAGGTGATTGTG
TTTGCAGTTGCGCTGGGTATTGCTTTAGTACTGATTGGTGAACATGGTAAGCCTGCTATCAAGGTGTTCGAAAGCCTAGC
CGAAGCCATGTATAAGCTGACTGACTTAGTAATGAAGTTTGCACCATACGGCGTATTTGGCCTGATGGCATGGGTTGCTG
GCGAGTATGGTGTCGACATGTTGATGCCGTTAATAAAGGTTATTATCGCTGTTTATATTGGCTGTTTCCTGCATGTTGTT
GGCTTCTATAGCTTCGTTTTAGTGGTATTCGCTAAGCTTAACCCTATTCAATTCTTTAAAGGCATTAGTAATGCGATTGC
GGTGGCTTATACTACTTCGAGCTCTGCGGGTACGCTGCCAGCGAGTATGAAGTGTGCCAGTGAGTACTTAGGCGTTAATA
AGAAGATCTCAAGCTTCGTATTGCCATTAGGTACCACCATCAACATGGATGGTACCGCGCTTTATCAAGGTGTTACGGCA
TTGTTTGTGGCACAAGCTTTCGGTATCGATCTGACTTGGGTCGATTACATCACTATCATCTTGACTGCAACGCTAGCCTC
TATTGGTACAGCGGGTGTGCCGGGTGCGGGTCTTGTGATGTTGACGCTAGTATTGACCACTGTGGGTCTACCACTTGAAG
GCGTTGCCCTTATTGCCGGTATCGACCGAATTTTGGATATGGCGCGTACCGTAGTAAACGTCTCTGGTGACTTAGTAGCC
ACCACAGTTATTGCTAAGTCTGAAGGTGAGATTGACGTTGAGCACTACAATGCTAACGCAGAAGACAGTGCAGAAATGGC
TGAAAAGCTAAACTCTTAA

Upstream 100 bases:

>100_bases
GATCATCTCTCACTATTGCAGCATATGCTAAACTGCCGCGTTATTTTTTAGGCTGGCGACTTGCTGGCTGTCCTTCCTCC
CACTATTTCAAGGTCTAAAT

Downstream 100 bases:

>100_bases
GCCAAGTGAGCTAAGTTTATGTCCACCGCTAATGGTGGGCCAATAAAAAACCGCCGAAAGGCGGTTTTTTATTTCTGGAA
CATTTATGCCGATTTACCAT

Product: sodium:dicarboxylate symporter

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate-aspartate carrier protein [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSNQQPSLLKKVWSSWMSVPLWLQIMIGMLLGILTGVSLGEQATYLKPIGTLFVNTIKMLIVPLVFCSLIVGVTSMQDTA
KMGRIGFKSFAFYLGTTSIAITLGLAVGHIMQPGTGLDMVAHETAEVVKQAPSIMQTLIDIVPTNPIAALASGQILQVIV
FAVALGIALVLIGEHGKPAIKVFESLAEAMYKLTDLVMKFAPYGVFGLMAWVAGEYGVDMLMPLIKVIIAVYIGCFLHVV
GFYSFVLVVFAKLNPIQFFKGISNAIAVAYTTSSSAGTLPASMKCASEYLGVNKKISSFVLPLGTTINMDGTALYQGVTA
LFVAQAFGIDLTWVDYITIILTATLASIGTAGVPGAGLVMLTLVLTTVGLPLEGVALIAGIDRILDMARTVVNVSGDLVA
TTVIAKSEGEIDVEHYNANAEDSAEMAEKLNS

Sequences:

>Translated_432_residues
MSNQQPSLLKKVWSSWMSVPLWLQIMIGMLLGILTGVSLGEQATYLKPIGTLFVNTIKMLIVPLVFCSLIVGVTSMQDTA
KMGRIGFKSFAFYLGTTSIAITLGLAVGHIMQPGTGLDMVAHETAEVVKQAPSIMQTLIDIVPTNPIAALASGQILQVIV
FAVALGIALVLIGEHGKPAIKVFESLAEAMYKLTDLVMKFAPYGVFGLMAWVAGEYGVDMLMPLIKVIIAVYIGCFLHVV
GFYSFVLVVFAKLNPIQFFKGISNAIAVAYTTSSSAGTLPASMKCASEYLGVNKKISSFVLPLGTTINMDGTALYQGVTA
LFVAQAFGIDLTWVDYITIILTATLASIGTAGVPGAGLVMLTLVLTTVGLPLEGVALIAGIDRILDMARTVVNVSGDLVA
TTVIAKSEGEIDVEHYNANAEDSAEMAEKLNS
>Mature_431_residues
SNQQPSLLKKVWSSWMSVPLWLQIMIGMLLGILTGVSLGEQATYLKPIGTLFVNTIKMLIVPLVFCSLIVGVTSMQDTAK
MGRIGFKSFAFYLGTTSIAITLGLAVGHIMQPGTGLDMVAHETAEVVKQAPSIMQTLIDIVPTNPIAALASGQILQVIVF
AVALGIALVLIGEHGKPAIKVFESLAEAMYKLTDLVMKFAPYGVFGLMAWVAGEYGVDMLMPLIKVIIAVYIGCFLHVVG
FYSFVLVVFAKLNPIQFFKGISNAIAVAYTTSSSAGTLPASMKCASEYLGVNKKISSFVLPLGTTINMDGTALYQGVTAL
FVAQAFGIDLTWVDYITIILTATLASIGTAGVPGAGLVMLTLVLTTVGLPLEGVALIAGIDRILDMARTVVNVSGDLVAT
TVIAKSEGEIDVEHYNANAEDSAEMAEKLNS

Specific function: This carrier protein is part of the Na(+)-dependent, binding-protein-independent glutamate-aspartate transport system [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI21314632, Length=430, Percent_Identity=31.8604651162791, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI40254478, Length=446, Percent_Identity=31.8385650224215, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI169790839, Length=444, Percent_Identity=30.8558558558559, Blast_Score=192, Evalue=7e-49,
Organism=Homo sapiens, GI66773030, Length=475, Percent_Identity=32, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI5032093, Length=375, Percent_Identity=35.7333333333333, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI4827012, Length=302, Percent_Identity=36.7549668874172, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI223468564, Length=250, Percent_Identity=40.4, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI223468566, Length=250, Percent_Identity=40.4, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI194239697, Length=255, Percent_Identity=39.2156862745098, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI262359914, Length=378, Percent_Identity=27.7777777777778, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI301601644, Length=197, Percent_Identity=36.0406091370558, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1790514, Length=411, Percent_Identity=38.9294403892944, Blast_Score=275, Evalue=5e-75,
Organism=Escherichia coli, GI1789947, Length=415, Percent_Identity=35.9036144578313, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1788024, Length=412, Percent_Identity=28.1553398058252, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1789473, Length=376, Percent_Identity=26.8617021276596, Blast_Score=99, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71996953, Length=469, Percent_Identity=35.181236673774, Blast_Score=232, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI71983099, Length=474, Percent_Identity=33.1223628691983, Blast_Score=215, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI71983106, Length=468, Percent_Identity=33.3333333333333, Blast_Score=212, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17537407, Length=458, Percent_Identity=32.3144104803493, Blast_Score=200, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI193206505, Length=460, Percent_Identity=31.0869565217391, Blast_Score=194, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI193206654, Length=427, Percent_Identity=30.4449648711944, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17541374, Length=392, Percent_Identity=29.3367346938776, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24583025, Length=446, Percent_Identity=30.4932735426009, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI17137668, Length=446, Percent_Identity=30.4932735426009, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24583023, Length=446, Percent_Identity=30.4932735426009, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI281360481, Length=461, Percent_Identity=29.0672451193059, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI281360483, Length=461, Percent_Identity=29.0672451193059, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI17137666, Length=461, Percent_Identity=29.0672451193059, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 45742; Mature: 45611

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHHHCC
>Mature Secondary Structure 
SNQQPSLLKKVWSSWMSVPLWLQIMIGMLLGILTGVSLGEQATYLKPIGTLFVNTIKML
CCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH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CHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]