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The map label for this gene is nagB [H]

Identifier: 157960588

GI number: 157960588

Start: 945297

End: 946088

Strand: Direct

Name: nagB [H]

Synonym: Spea_0760

Alternate gene names: 157960588

Gene position: 945297-946088 (Clockwise)

Preceding gene: 157960586

Following gene: 157960589

Centisome position: 18.27

GC content: 46.09

Gene sequence:

>792_bases
ATGCAAATCGTAATATTAAAAGACAGTGCGTCGGTTGCTGAATATGGCGCAAATATTTTTATTAAGCAGCTGAAGAAGAA
GGCGGATTCTGTGCTTGGCTTGGCGACAGGTTCAACCCCTGTGTCCCTATATCAAAGTTTAATTGCCGCAAATAAGGCTA
AGCAGATCTCCTTTAAAGCCGTTACAACGTTCAATCTAGATGAATACCTTGGCCTTGAAGGCACGCATCCACAAAGTTAT
CGTTACTTTATGAATGAGCAGCTTTTTGAGCATATCGATATCGATAAATCTAAGACTTTTGTGCCGCCGGGAGATGCCGC
TAATCCGCTTGAAGCCTGCCAAGCTTATGAGGAAAAGATAAAATCAGCGGGCGGTATCGATATCCAGTTACTCGGCATTG
GCCGTAACGGTCATATCGGTTTTAACGAGCCCTCATCAGGCTTAATGTCGCGTACTCGTGTGAAGACCTTAACCAAGGCG
ACTATCGAAGATAACGCCCGTTTCTTCAAAGAAGATGAGTATCAACCACATCTGTCGATCACTATGGGCATAGGCACCAT
CTTAGATGCCAAGAAAGTAGTGCTATTGGCCACCGGCGAGAATAAGGCCGATGCCATTCTTGCTACAGTGGAAGGCGCAT
TAACCGCAGCTTGCCCTGCATCGGCACTGCAACTGCATCAAGATGCGGTATTGGTGATCGATGAGGCGGCGGCTTCAAAA
CTGTCTGACAAAGATTTTTATAAGCACATCGAAGCCGAGAACCAAAAGCTGATGGCGAGATTGGGTCTGTAA

Upstream 100 bases:

>100_bases
TTCGGCAACGAATTAAGCAAACCTGTTGAATGAAGAGCTGCTTAGCTAGCCTTTAGCTAAAGACTGGCTAACAACGACGA
ACACATATTGGATCAAGTTT

Downstream 100 bases:

>100_bases
ATATGACGCCTTTGTTTTAATAGGGCTGCTCGGTATCGATTTATTGAAGGACAAGCATTAGCATGTCCTTTTTTGTTTTC
TGCCCTAAACCACGACTTCA

Product: glucosamine-6-phosphate isomerase

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MQIVILKDSASVAEYGANIFIKQLKKKADSVLGLATGSTPVSLYQSLIAANKAKQISFKAVTTFNLDEYLGLEGTHPQSY
RYFMNEQLFEHIDIDKSKTFVPPGDAANPLEACQAYEEKIKSAGGIDIQLLGIGRNGHIGFNEPSSGLMSRTRVKTLTKA
TIEDNARFFKEDEYQPHLSITMGIGTILDAKKVVLLATGENKADAILATVEGALTAACPASALQLHQDAVLVIDEAAASK
LSDKDFYKHIEAENQKLMARLGL

Sequences:

>Translated_263_residues
MQIVILKDSASVAEYGANIFIKQLKKKADSVLGLATGSTPVSLYQSLIAANKAKQISFKAVTTFNLDEYLGLEGTHPQSY
RYFMNEQLFEHIDIDKSKTFVPPGDAANPLEACQAYEEKIKSAGGIDIQLLGIGRNGHIGFNEPSSGLMSRTRVKTLTKA
TIEDNARFFKEDEYQPHLSITMGIGTILDAKKVVLLATGENKADAILATVEGALTAACPASALQLHQDAVLVIDEAAASK
LSDKDFYKHIEAENQKLMARLGL
>Mature_263_residues
MQIVILKDSASVAEYGANIFIKQLKKKADSVLGLATGSTPVSLYQSLIAANKAKQISFKAVTTFNLDEYLGLEGTHPQSY
RYFMNEQLFEHIDIDKSKTFVPPGDAANPLEACQAYEEKIKSAGGIDIQLLGIGRNGHIGFNEPSSGLMSRTRVKTLTKA
TIEDNARFFKEDEYQPHLSITMGIGTILDAKKVVLLATGENKADAILATVEGALTAACPASALQLHQDAVLVIDEAAASK
LSDKDFYKHIEAENQKLMARLGL

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion [H]

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily [H]

Homologues:

Organism=Homo sapiens, GI19923881, Length=269, Percent_Identity=42.0074349442379, Blast_Score=203, Evalue=1e-52,
Organism=Homo sapiens, GI13027378, Length=269, Percent_Identity=42.3791821561338, Blast_Score=201, Evalue=5e-52,
Organism=Escherichia coli, GI1786893, Length=264, Percent_Identity=42.8030303030303, Blast_Score=213, Evalue=9e-57,
Organism=Escherichia coli, GI1789530, Length=229, Percent_Identity=27.9475982532751, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI48994958, Length=244, Percent_Identity=23.7704918032787, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17554876, Length=249, Percent_Identity=41.3654618473896, Blast_Score=203, Evalue=7e-53,
Organism=Drosophila melanogaster, GI24581960, Length=250, Percent_Identity=42.8, Blast_Score=212, Evalue=2e-55,
Organism=Drosophila melanogaster, GI19920764, Length=250, Percent_Identity=42.8, Blast_Score=212, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321 [H]

Pfam domain/function: PF01182 Glucosamine_iso [H]

EC number: =3.5.99.6 [H]

Molecular weight: Translated: 28534; Mature: 28534

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIVILKDSASVAEYGANIFIKQLKKKADSVLGLATGSTPVSLYQSLIAANKAKQISFKA
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHCCCCCEEEEEE
VTTFNLDEYLGLEGTHPQSYRYFMNEQLFEHIDIDKSKTFVPPGDAANPLEACQAYEEKI
EEEECHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KSAGGIDIQLLGIGRNGHIGFNEPSSGLMSRTRVKTLTKATIEDNARFFKEDEYQPHLSI
HHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEEE
TMGIGTILDAKKVVLLATGENKADAILATVEGALTAACPASALQLHQDAVLVIDEAAASK
EECCHHHCCCCEEEEEEECCCCCCEEEEECCCHHHHCCCHHHHHHCCCEEEEEECHHHHC
LSDKDFYKHIEAENQKLMARLGL
CCHHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure
MQIVILKDSASVAEYGANIFIKQLKKKADSVLGLATGSTPVSLYQSLIAANKAKQISFKA
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHCCCCCEEEEEE
VTTFNLDEYLGLEGTHPQSYRYFMNEQLFEHIDIDKSKTFVPPGDAANPLEACQAYEEKI
EEEECHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KSAGGIDIQLLGIGRNGHIGFNEPSSGLMSRTRVKTLTKATIEDNARFFKEDEYQPHLSI
HHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEEE
TMGIGTILDAKKVVLLATGENKADAILATVEGALTAACPASALQLHQDAVLVIDEAAASK
EECCHHHCCCCEEEEEEECCCCCCEEEEECCCHHHHCCCHHHHHHCCCEEEEEECHHHHC
LSDKDFYKHIEAENQKLMARLGL
CCHHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA