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The map label for this gene is pepB [H]

Identifier: 157960528

GI number: 157960528

Start: 860251

End: 861528

Strand: Direct

Name: pepB [H]

Synonym: Spea_0700

Alternate gene names: 157960528

Gene position: 860251-861528 (Clockwise)

Preceding gene: 157960518

Following gene: 157960529

Centisome position: 16.62

GC content: 51.8

Gene sequence:

>1278_bases
ATGACGGATTTTATGAAAGTGACTCTAACTCACGAACCTGCTGCAGCTCACTGGGGCAGAGCAGATGTGACCTTTCAAGG
GGAGTTCGCCTCTATTCATATGACAGCGGGAGATGCCTTGCTGCATGTACAGCAAGCGGCGCGCAAGTTAGAGCTACAAG
GAGTGACTAAGGTCGCCCTAACTGGTGAGCTGTGGACTTTAGATCTACAGTGGGCTTTTGCCCAAGGTTTTGCCACAGCG
CTGACCACCCCGATGATTGAGTGGACTGGTGATGATGCAACTCAGGCGTTGTTAGCTAGTCGTCTAGAAAGCGCAACATT
TGCCCGTCACCTGATTAATGAAACCCCTGAAAACCTATCTCCAGTGGCGCTAGCGACTCAAGCGGCTAAATGGCTTAGCG
ATATCGGCGGTGACAAGGTGAGCTATCGTATTATCGAAGGCGAAGCACTATTAGAAGAGAAATGGATAGGTATTCACGCT
GTAGGCCGTGGTAGTGAGCGACCACCTGCGCTGCTTGAGTTAGACTTTAATCCATTAGCTAGCGACGCCGATGTCTCTGT
TGCCCTAGTGGGTAAAGGGATCACCTTTGACTCTGGCGGCTATAGCATCAAAGGCTCTGAAGGCATGCTTGGTATGAAAT
GTGACATGGGCGGCGCGGCCACAGTTACGGCAGCTCTTGGCCTTGCGATGAAATCAGGCCTAAACAAGCGTGTGAAACTG
TTCTTATGCTGCGCCGAAAACCTCATTAGTGGCCATGCCTATAAGCTGGGTGACATTCTTAAATATAAAAATGGCGTCAC
GGTTGAAGTGGTCAATACCGATGCCGAAGGTCGCTTGGTACTAGCCGATGGTTTACAAGCTGCCTCAGAAACGGGCGCAC
CAGTGATCATCGATGCGGCGACCCTAACGGGCGCCGCGGTTATGGCGGTAGGCACTAACTACAACGCTATTTTCTCGCCT
AAGCCTGAGATGCTAGCGTTAGCTCAGCAAAAAGCATTGGCTGTGGGTGAGCGTGTTTGGCCGTTACCACTGGATCCATG
GCATAAAGATAACTGCCCAAGTGCCTATGCCGATACGGCAAACAGTCGCCCGATGAAAGGTGGTGGAGCCGGTGGAGCAT
CGAACGCAGCCGGCTTCCTATGGCGTTTTGTCGCCCCAGAAGCTAACTGGTTACATATTGATTTAGCCTCTGCGTTTTCT
GACTCGGGTGACAATCTATGGTCAGCAGGCGCGACGACCCACGGCGTATTGACTATTGCCGGTCTTTTAGAGGGCTAA

Upstream 100 bases:

>100_bases
TCCAGTAGACTGCTTGCACTAAAAATAGGCTGGTTGTGGGTAATAGCGGCTGTAAAGCGACTGTTATTGGCAACATCGAT
TTATATAATAAGGACCTAGC

Downstream 100 bases:

>100_bases
TAACTAGTACTAAAGGCTGATATTGATGCTTAGTAAAAGCTAATGTATTGGCTGATATTTAAATTTGATCAAGCAGGCCA
ATATCGCCTCAGTCAGTCTT

Product: aminopeptidase B

Products: NA

Alternate protein names: Aminopeptidase B [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTDFMKVTLTHEPAAAHWGRADVTFQGEFASIHMTAGDALLHVQQAARKLELQGVTKVALTGELWTLDLQWAFAQGFATA
LTTPMIEWTGDDATQALLASRLESATFARHLINETPENLSPVALATQAAKWLSDIGGDKVSYRIIEGEALLEEKWIGIHA
VGRGSERPPALLELDFNPLASDADVSVALVGKGITFDSGGYSIKGSEGMLGMKCDMGGAATVTAALGLAMKSGLNKRVKL
FLCCAENLISGHAYKLGDILKYKNGVTVEVVNTDAEGRLVLADGLQAASETGAPVIIDAATLTGAAVMAVGTNYNAIFSP
KPEMLALAQQKALAVGERVWPLPLDPWHKDNCPSAYADTANSRPMKGGGAGGASNAAGFLWRFVAPEANWLHIDLASAFS
DSGDNLWSAGATTHGVLTIAGLLEG

Sequences:

>Translated_425_residues
MTDFMKVTLTHEPAAAHWGRADVTFQGEFASIHMTAGDALLHVQQAARKLELQGVTKVALTGELWTLDLQWAFAQGFATA
LTTPMIEWTGDDATQALLASRLESATFARHLINETPENLSPVALATQAAKWLSDIGGDKVSYRIIEGEALLEEKWIGIHA
VGRGSERPPALLELDFNPLASDADVSVALVGKGITFDSGGYSIKGSEGMLGMKCDMGGAATVTAALGLAMKSGLNKRVKL
FLCCAENLISGHAYKLGDILKYKNGVTVEVVNTDAEGRLVLADGLQAASETGAPVIIDAATLTGAAVMAVGTNYNAIFSP
KPEMLALAQQKALAVGERVWPLPLDPWHKDNCPSAYADTANSRPMKGGGAGGASNAAGFLWRFVAPEANWLHIDLASAFS
DSGDNLWSAGATTHGVLTIAGLLEG
>Mature_424_residues
TDFMKVTLTHEPAAAHWGRADVTFQGEFASIHMTAGDALLHVQQAARKLELQGVTKVALTGELWTLDLQWAFAQGFATAL
TTPMIEWTGDDATQALLASRLESATFARHLINETPENLSPVALATQAAKWLSDIGGDKVSYRIIEGEALLEEKWIGIHAV
GRGSERPPALLELDFNPLASDADVSVALVGKGITFDSGGYSIKGSEGMLGMKCDMGGAATVTAALGLAMKSGLNKRVKLF
LCCAENLISGHAYKLGDILKYKNGVTVEVVNTDAEGRLVLADGLQAASETGAPVIIDAATLTGAAVMAVGTNYNAIFSPK
PEMLALAQQKALAVGERVWPLPLDPWHKDNCPSAYADTANSRPMKGGGAGGASNAAGFLWRFVAPEANWLHIDLASAFSD
SGDNLWSAGATTHGVLTIAGLLEG

Specific function: Probably plays an important role in intracellular peptide degradation [H]

COG id: COG0260

COG function: function code E; Leucyl aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M17 family [H]

Homologues:

Organism=Homo sapiens, GI41393561, Length=302, Percent_Identity=36.0927152317881, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI47155554, Length=262, Percent_Identity=40.4580152671756, Blast_Score=145, Evalue=9e-35,
Organism=Escherichia coli, GI87082123, Length=429, Percent_Identity=49.6503496503497, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI1790710, Length=315, Percent_Identity=39.0476190476191, Blast_Score=170, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17556903, Length=316, Percent_Identity=36.7088607594937, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17565172, Length=296, Percent_Identity=26.3513513513513, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21357381, Length=277, Percent_Identity=37.1841155234657, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI221379063, Length=276, Percent_Identity=37.6811594202899, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI221379062, Length=276, Percent_Identity=37.6811594202899, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24661038, Length=301, Percent_Identity=31.8936877076412, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21355725, Length=297, Percent_Identity=31.986531986532, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI20129969, Length=368, Percent_Identity=26.6304347826087, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24662227, Length=297, Percent_Identity=28.2828282828283, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI161077148, Length=368, Percent_Identity=28.5326086956522, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI20130057, Length=368, Percent_Identity=28.5326086956522, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21355645, Length=332, Percent_Identity=26.8072289156627, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24662223, Length=332, Percent_Identity=26.8072289156627, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI20129963, Length=366, Percent_Identity=27.8688524590164, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19922386, Length=368, Percent_Identity=26.9021739130435, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24646701, Length=286, Percent_Identity=27.6223776223776, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24646703, Length=286, Percent_Identity=27.6223776223776, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21358201, Length=286, Percent_Identity=27.6223776223776, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008330
- InterPro:   IPR011356
- InterPro:   IPR000819 [H]

Pfam domain/function: PF00883 Peptidase_M17 [H]

EC number: =3.4.11.23 [H]

Molecular weight: Translated: 44685; Mature: 44554

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00631 CYTOSOL_AP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFMKVTLTHEPAAAHWGRADVTFQGEFASIHMTAGDALLHVQQAARKLELQGVTKVAL
CCCEEEEEEECCCCCCCCCCCEEEEECCEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEE
TGELWTLDLQWAFAQGFATALTTPMIEWTGDDATQALLASRLESATFARHLINETPENLS
ECCEEEEEEEEHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PVALATQAAKWLSDIGGDKVSYRIIEGEALLEEKWIGIHAVGRGSERPPALLELDFNPLA
CHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHCCEEEEEECCCCCCCCEEEEECCCCCC
SDADVSVALVGKGITFDSGGYSIKGSEGMLGMKCDMGGAATVTAALGLAMKSGLNKRVKL
CCCCEEEEEEECCEEECCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEE
FLCCAENLISGHAYKLGDILKYKNGVTVEVVNTDAEGRLVLADGLQAASETGAPVIIDAA
EEEEHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCEEEEECCCHHHHCCCCCEEEEEC
TLTGAAVMAVGTNYNAIFSPKPEMLALAQQKALAVGERVWPLPLDPWHKDNCPSAYADTA
HHCCEEEEEECCCCCEEECCCHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCHHHCCC
NSRPMKGGGAGGASNAAGFLWRFVAPEANWLHIDLASAFSDSGDNLWSAGATTHGVLTIA
CCCCCCCCCCCCCCCCHHEEEEEECCCCCEEEEEEHHHCCCCCCCEEECCCCCCCCEEEE
GLLEG
EECCC
>Mature Secondary Structure 
TDFMKVTLTHEPAAAHWGRADVTFQGEFASIHMTAGDALLHVQQAARKLELQGVTKVAL
CCEEEEEEECCCCCCCCCCCEEEEECCEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEE
TGELWTLDLQWAFAQGFATALTTPMIEWTGDDATQALLASRLESATFARHLINETPENLS
ECCEEEEEEEEHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PVALATQAAKWLSDIGGDKVSYRIIEGEALLEEKWIGIHAVGRGSERPPALLELDFNPLA
CHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHCCEEEEEECCCCCCCCEEEEECCCCCC
SDADVSVALVGKGITFDSGGYSIKGSEGMLGMKCDMGGAATVTAALGLAMKSGLNKRVKL
CCCCEEEEEEECCEEECCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEE
FLCCAENLISGHAYKLGDILKYKNGVTVEVVNTDAEGRLVLADGLQAASETGAPVIIDAA
EEEEHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCEEEEECCCHHHHCCCCCEEEEEC
TLTGAAVMAVGTNYNAIFSPKPEMLALAQQKALAVGERVWPLPLDPWHKDNCPSAYADTA
HHCCEEEEEECCCCCEEECCCHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCHHHCCC
NSRPMKGGGAGGASNAAGFLWRFVAPEANWLHIDLASAFSDSGDNLWSAGATTHGVLTIA
CCCCCCCCCCCCCCCCHHEEEEEECCCCCEEEEEEHHHCCCCCCCEEECCCCCCCCEEEE
GLLEG
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA