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The map label for this gene is mpl [H]

Identifier: 157960514

GI number: 157960514

Start: 847864

End: 849249

Strand: Direct

Name: mpl [H]

Synonym: Spea_0686

Alternate gene names: 157960514

Gene position: 847864-849249 (Clockwise)

Preceding gene: 157960508

Following gene: 157960515

Centisome position: 16.39

GC content: 48.41

Gene sequence:

>1386_bases
ATGCACGTACATATTTTAGGAATTTGTGGCACTTTCATGGGTGGCTTAGCCTTATTAGCTAGAGCAGAAGGGCACAAGGT
CACAGGCAGCGATGCCAATGTTTATCCTCCGATGAGCACGCAACTCGAAGAGCAAGGGATTGAGCTTATTCAGGGCTTCG
ATCCATCACAACTCGGAAGCAGTGAGGATGATGCGCCAGATCTTGTGGTAATTGGCAATGCCATGAGCCGTGGCAATCCT
TGTGTCGAGGCGGTATTGAACCGCGGCCTTAAATACACCTCTGGGCCACAGTTTCTTGCCGAGCATATCTTGCCTCAGCG
CTGGGTATTGGCAGTAGCGGGCACCCACGGCAAGACATCGACATCGAGTATGTTGGCGTGGATCTTAGAAGATTGCGGCT
ATGAGCCAGGCTTTTTGATTGGCGGCGTGCCACAAAACTTCGGTGTGTCTGCTCGCTTAGGTGGCTCGCCTTTCTTTGTA
GTGGAAGCCGACGAGTATGACAGCGCGTTTTTCGATAAGCGTTCTAAGTTTGTGCACTACCAGCCACGCACCTTAGTGAT
TAATAACTTAGAGTTTGATCATGCGGATATTTTTGATGATTTAAAGGCGATTCAGCGTCAGTTTAACCATGTCATACGTA
CCGTGCCGGGTGAGGGTAAGATTATCTGGCCTGCGGATGCTAATAGTGTGCAACAAGTTATCGATATGGGCTGCTGGAGT
GAGCAAGAGCAGTATCACTTGAGTGGCAACTCACAGGGCTGGTATGCGGTTAACTTGACTGAAGACGGTCATAAATTTGA
GGTGTTCTTCGATGGTGAGTCGCAAGGCATTTTGGACTGGCAGTTAATCGGTCAGCACAATATTGAAAATGCGGTGATGG
CTATTGCTGCAGCAAGGCATGTGGGGGTTAAACCAGAGGCGGCAATTGCCGCCTTAACCCAGTTTGCACCACCAAAGCGC
AGAATGGAGCTACTAGATACAGTCAATGGTATCGAAGTCTATGATGACTTTGCTCATCACCCGACAGCCATTACCACTTC
GCTCAATGGCATGCGAGCCCGTGTTGGGAGCGGCAAGATCACTGTGATTTTAGAGCCGCGCTCTAACACCATGAAGAGCG
GAGTACATAAAGATACTCTAGCTAACTCACTGAGCTTGGCGAGCAATGCATACTTGTATCAAGCGGGTAATATCGATTGG
GATATTAGCGCTGCGATGAGCTGCGCCTCGATCCCGGTTGAGGTCTTATATGATATTGATGAGATTGTTGAGCGGGTATC
TGCCCAAGCTAATAGCACAGATACTATTATTGTTATGAGTAATGGTGGCTTTGGTGGGTTACATCAAAAGCTGCTTGCTA
AGCTTCGTCATCAAACAGGTAAATAA

Upstream 100 bases:

>100_bases
CCGATACAGACAGGAGCAATTTTAAACTTATAGTTAACTGACAAAACTCGTTGCAATTGGTTAGAATGAGGCAGATTTTT
CATTCTTCAGAGTGGCAGGC

Downstream 100 bases:

>100_bases
ATAGCCATGAGTGGGACATATCGTAAAGCAGACAAATCGATTAGTTTAGCCTGGACCGGCGCATCAGGCGCTCCCTATGG
CTTAAGGCTGCTTGAAATTT

Product: UDP-N-acetylmuramate

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MHVHILGICGTFMGGLALLARAEGHKVTGSDANVYPPMSTQLEEQGIELIQGFDPSQLGSSEDDAPDLVVIGNAMSRGNP
CVEAVLNRGLKYTSGPQFLAEHILPQRWVLAVAGTHGKTSTSSMLAWILEDCGYEPGFLIGGVPQNFGVSARLGGSPFFV
VEADEYDSAFFDKRSKFVHYQPRTLVINNLEFDHADIFDDLKAIQRQFNHVIRTVPGEGKIIWPADANSVQQVIDMGCWS
EQEQYHLSGNSQGWYAVNLTEDGHKFEVFFDGESQGILDWQLIGQHNIENAVMAIAAARHVGVKPEAAIAALTQFAPPKR
RMELLDTVNGIEVYDDFAHHPTAITTSLNGMRARVGSGKITVILEPRSNTMKSGVHKDTLANSLSLASNAYLYQAGNIDW
DISAAMSCASIPVEVLYDIDEIVERVSAQANSTDTIIVMSNGGFGGLHQKLLAKLRHQTGK

Sequences:

>Translated_461_residues
MHVHILGICGTFMGGLALLARAEGHKVTGSDANVYPPMSTQLEEQGIELIQGFDPSQLGSSEDDAPDLVVIGNAMSRGNP
CVEAVLNRGLKYTSGPQFLAEHILPQRWVLAVAGTHGKTSTSSMLAWILEDCGYEPGFLIGGVPQNFGVSARLGGSPFFV
VEADEYDSAFFDKRSKFVHYQPRTLVINNLEFDHADIFDDLKAIQRQFNHVIRTVPGEGKIIWPADANSVQQVIDMGCWS
EQEQYHLSGNSQGWYAVNLTEDGHKFEVFFDGESQGILDWQLIGQHNIENAVMAIAAARHVGVKPEAAIAALTQFAPPKR
RMELLDTVNGIEVYDDFAHHPTAITTSLNGMRARVGSGKITVILEPRSNTMKSGVHKDTLANSLSLASNAYLYQAGNIDW
DISAAMSCASIPVEVLYDIDEIVERVSAQANSTDTIIVMSNGGFGGLHQKLLAKLRHQTGK
>Mature_461_residues
MHVHILGICGTFMGGLALLARAEGHKVTGSDANVYPPMSTQLEEQGIELIQGFDPSQLGSSEDDAPDLVVIGNAMSRGNP
CVEAVLNRGLKYTSGPQFLAEHILPQRWVLAVAGTHGKTSTSSMLAWILEDCGYEPGFLIGGVPQNFGVSARLGGSPFFV
VEADEYDSAFFDKRSKFVHYQPRTLVINNLEFDHADIFDDLKAIQRQFNHVIRTVPGEGKIIWPADANSVQQVIDMGCWS
EQEQYHLSGNSQGWYAVNLTEDGHKFEVFFDGESQGILDWQLIGQHNIENAVMAIAAARHVGVKPEAAIAALTQFAPPKR
RMELLDTVNGIEVYDDFAHHPTAITTSLNGMRARVGSGKITVILEPRSNTMKSGVHKDTLANSLSLASNAYLYQAGNIDW
DISAAMSCASIPVEVLYDIDEIVERVSAQANSTDTIIVMSNGGFGGLHQKLLAKLRHQTGK

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=456, Percent_Identity=60.7456140350877, Blast_Score=570, Evalue=1e-164,
Organism=Escherichia coli, GI1786279, Length=461, Percent_Identity=26.0303687635575, Blast_Score=113, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 50220; Mature: 50220

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVHILGICGTFMGGLALLARAEGHKVTGSDANVYPPMSTQLEEQGIELIQGFDPSQLGS
CEEEEEEHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCC
SEDDAPDLVVIGNAMSRGNPCVEAVLNRGLKYTSGPQFLAEHILPQRWVLAVAGTHGKTS
CCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCC
TSSMLAWILEDCGYEPGFLIGGVPQNFGVSARLGGSPFFVVEADEYDSAFFDKRSKFVHY
HHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEECCCEEEEEECCCCCHHHHHCCCCEEEE
QPRTLVINNLEFDHADIFDDLKAIQRQFNHVIRTVPGEGKIIWPADANSVQQVIDMGCWS
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCC
EQEQYHLSGNSQGWYAVNLTEDGHKFEVFFDGESQGILDWQLIGQHNIENAVMAIAAARH
CCCEEEECCCCCCEEEEEEECCCCEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHH
VGVKPEAAIAALTQFAPPKRRMELLDTVNGIEVYDDFAHHPTAITTSLNGMRARVGSGKI
CCCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCEEEECCCCCEEECCCCEE
TVILEPRSNTMKSGVHKDTLANSLSLASNAYLYQAGNIDWDISAAMSCASIPVEVLYDID
EEEECCCCCHHHCCCCHHHHHHHHHHCCCEEEEECCCCCEEHHHHHHHHCCCHHHHHHHH
EIVERVSAQANSTDTIIVMSNGGFGGLHQKLLAKLRHQTGK
HHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHVHILGICGTFMGGLALLARAEGHKVTGSDANVYPPMSTQLEEQGIELIQGFDPSQLGS
CEEEEEEHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCC
SEDDAPDLVVIGNAMSRGNPCVEAVLNRGLKYTSGPQFLAEHILPQRWVLAVAGTHGKTS
CCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCC
TSSMLAWILEDCGYEPGFLIGGVPQNFGVSARLGGSPFFVVEADEYDSAFFDKRSKFVHY
HHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEECCCEEEEEECCCCCHHHHHCCCCEEEE
QPRTLVINNLEFDHADIFDDLKAIQRQFNHVIRTVPGEGKIIWPADANSVQQVIDMGCWS
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCC
EQEQYHLSGNSQGWYAVNLTEDGHKFEVFFDGESQGILDWQLIGQHNIENAVMAIAAARH
CCCEEEECCCCCCEEEEEEECCCCEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHH
VGVKPEAAIAALTQFAPPKRRMELLDTVNGIEVYDDFAHHPTAITTSLNGMRARVGSGKI
CCCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCEEEECCCCCEEECCCCEE
TVILEPRSNTMKSGVHKDTLANSLSLASNAYLYQAGNIDWDISAAMSCASIPVEVLYDID
EEEECCCCCHHHCCCCHHHHHHHHHHCCCEEEEECCCCCEEHHHHHHHHCCCHHHHHHHH
EIVERVSAQANSTDTIIVMSNGGFGGLHQKLLAKLRHQTGK
HHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]