Click here to switch to the map view.

The map label for this gene is yneF [H]

Identifier: 157960507

GI number: 157960507

Start: 839735

End: 841789

Strand: Direct

Name: yneF [H]

Synonym: Spea_0679

Alternate gene names: 157960507

Gene position: 839735-841789 (Clockwise)

Preceding gene: 157960506

Following gene: 157960508

Centisome position: 16.23

GC content: 44.53

Gene sequence:

>2055_bases
ATGATATTGTTTTTTCCGGCTCTACTCGGCTATTACCGTCTTTGCTTTTCCTTGCAACTAGCACCTTCTCTTAAACCTAG
CACCTTCTGTAAAATCCCCTCTGTAAATCTGGTAATTCAATGTGTTAAGCTTGTTGGCGTTGATTGTAAACAAACTGGAT
TCCGGGTGTACATTGCAAAAAAATGGCTAAAGATCATTACGCTCTTTATGGTGGCAAGTTCTGCCTCCGTTATGAGTCTT
CAGGTGCACGCAGCCAGCCCCTTTCTTAAGTTAGAGCAACAGCTTTCTGACTCATCTCCAAGTGATGCCCTGACACTGCT
CAACGAGTTTCAACTCGAGCCTATTAGCACCACGGCATACAACAGGGCTAAGTTACATTTTCTCTACGGCAAGCAATACG
CACGTAACCGCCTGCTTGAGCAATCGATTGCAAGCTACGACATGGCAATAGTGCTGGCTAAGCCACTCATGGGCTCAGAA
TTGCTAATTGAGAGCTATCTAGGGCGTTCTTTTGCACGCTATTTACAGACGAACGATCCTGAAGTTTATTGTAGCGACAG
GGCAAAGGCGTTGGTGCTAGCTAGGCGGCAAAGCAACAGAGAGTTATTAGCTAAGAGCTTAACTCAGAATGCTTTTTGCT
TTAATAAGGTCACGAATGTGCATCATGGCATCGCTCTGCTAGATGAAGCCATGTTGTTAATCGATACTGAAGACAAAGCG
AATTATAACCGTAAGGCAATGATCTTTAATGCCACCGGTTCACTCTATCGTACGGTAGGTCTACATAAACGTGGTTATGT
GAACTTTGAGAAAGCCTATCAAAATTGGAGCGAGATAGACGATACCCAAGACATGTTTAATATGCTGCATAACATGATCA
GCGAAGCGGTTAAGCTGGGAGATTGGGAAAAGGCGCGAGATAATATTGAACAGCAGTTTTTACTGGCGGTTAAAGCTTCT
GAATTTAACGACTTTGGTTTTTTCGCTCACCTAAATGCGGGGCGCGTAGAATTAGCCACCCATAATTATGTCGATGCGAT
TGAGCACTTAGAGCAAGCCATCTCCCTCAAGGACACCACCCGAGAACAATATTTTATCACGAGTAGTTACCTGTTTCTGG
CCCAGGCATATTTACGAAGTGGTGATGTAGAGCAAGCGGCGGCCATGGCACGCATTTTTAAGCAAGACAGCACATTCCCT
AAGAATATGAGCAGTATGACGCTCAAGGCTGATGCCATTATAGCCTTGGAGCAGCAGCAGTATTTATCTGCGATAAACGC
CTTGCTAGAGGTGATTGATAAGGAACGGCAAAAGAATAAACAGATCATAGATAATGAGGTGATCGACTTGGCGTTAGAGC
ATAACTCTACACTCGCCGAATTTGAGAATCAGTTACTGGCGAACAAATTGGCCATTAACGAATTGAGCCTAGCGGCCGTG
GCAGATAAACAGCGGATCTATGACTTAAGGGTGAGCCTCTTCTTCTTGGTAGCTACGGTACTTCTGATTGCAATTTTGTT
TCTGTGGCAATCTCGTAAAGCCTTTAAACACAGTGCACAAACTGACTTCTTAACTGGTATCGCTAATCGCGCTCATGTGT
TCAACCGAGGCCAGAGAATGATCGACAGGGCTATCAAGAAGAAGCATCCCTTAGCGGTGATCATTTTTGATATCGATAAC
TTTAAGTCTATTAACGATCAGTATGGCCATCAGGTGGGCGATCTGGCGATAAAAGCTGTTGCCAAGCGCGGAGAGCGTTG
GCTCAGAGATTGCGATCTTATTGGCCGAATCGGCGGCGAAGAATTTTTAATTGTACTGCCAGATGTCACTGAGGCTGACG
CGATTCTTATCTCTGAACGTTTACGTGAAGGCATAGCCTCTCAGGCATTTAAGTTCGATGATGTGGTTATCGAACTGACG
GTAAGTATAGGTGTGGCTGCATTAAAACCGCCTCAAACTCGCTTAACCGATCTGGTTAATCTGGCAGATAAGGGACTCTA
TAAGGCCAAGTTCTCTGGTAAGAATAGAGTCTATCTTATTGAACAGTTTGCTTAA

Upstream 100 bases:

>100_bases
CGGCTGAGCAATTTGAGCCGAAAATGCTTGATGAGATATAACTTGTCCCTAGGATCTAGAACCTACTTTTAATCTCTGAC
GGCATAAATGCCGAGCTAGA

Downstream 100 bases:

>100_bases
GCCTAACCCGCCTAACGCTTAGTCTTGTGCTAGGCTTTTTTGCTTCCCCCTATTCGGCCTAATGGCCCTTCTTTTAATGA
TTTACTGGGATTCAAACTTG

Product: TPR repeat-containing diguanylate cyclase and serine/threonine protein kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 684; Mature: 684

Protein sequence:

>684_residues
MILFFPALLGYYRLCFSLQLAPSLKPSTFCKIPSVNLVIQCVKLVGVDCKQTGFRVYIAKKWLKIITLFMVASSASVMSL
QVHAASPFLKLEQQLSDSSPSDALTLLNEFQLEPISTTAYNRAKLHFLYGKQYARNRLLEQSIASYDMAIVLAKPLMGSE
LLIESYLGRSFARYLQTNDPEVYCSDRAKALVLARRQSNRELLAKSLTQNAFCFNKVTNVHHGIALLDEAMLLIDTEDKA
NYNRKAMIFNATGSLYRTVGLHKRGYVNFEKAYQNWSEIDDTQDMFNMLHNMISEAVKLGDWEKARDNIEQQFLLAVKAS
EFNDFGFFAHLNAGRVELATHNYVDAIEHLEQAISLKDTTREQYFITSSYLFLAQAYLRSGDVEQAAAMARIFKQDSTFP
KNMSSMTLKADAIIALEQQQYLSAINALLEVIDKERQKNKQIIDNEVIDLALEHNSTLAEFENQLLANKLAINELSLAAV
ADKQRIYDLRVSLFFLVATVLLIAILFLWQSRKAFKHSAQTDFLTGIANRAHVFNRGQRMIDRAIKKKHPLAVIIFDIDN
FKSINDQYGHQVGDLAIKAVAKRGERWLRDCDLIGRIGGEEFLIVLPDVTEADAILISERLREGIASQAFKFDDVVIELT
VSIGVAALKPPQTRLTDLVNLADKGLYKAKFSGKNRVYLIEQFA

Sequences:

>Translated_684_residues
MILFFPALLGYYRLCFSLQLAPSLKPSTFCKIPSVNLVIQCVKLVGVDCKQTGFRVYIAKKWLKIITLFMVASSASVMSL
QVHAASPFLKLEQQLSDSSPSDALTLLNEFQLEPISTTAYNRAKLHFLYGKQYARNRLLEQSIASYDMAIVLAKPLMGSE
LLIESYLGRSFARYLQTNDPEVYCSDRAKALVLARRQSNRELLAKSLTQNAFCFNKVTNVHHGIALLDEAMLLIDTEDKA
NYNRKAMIFNATGSLYRTVGLHKRGYVNFEKAYQNWSEIDDTQDMFNMLHNMISEAVKLGDWEKARDNIEQQFLLAVKAS
EFNDFGFFAHLNAGRVELATHNYVDAIEHLEQAISLKDTTREQYFITSSYLFLAQAYLRSGDVEQAAAMARIFKQDSTFP
KNMSSMTLKADAIIALEQQQYLSAINALLEVIDKERQKNKQIIDNEVIDLALEHNSTLAEFENQLLANKLAINELSLAAV
ADKQRIYDLRVSLFFLVATVLLIAILFLWQSRKAFKHSAQTDFLTGIANRAHVFNRGQRMIDRAIKKKHPLAVIIFDIDN
FKSINDQYGHQVGDLAIKAVAKRGERWLRDCDLIGRIGGEEFLIVLPDVTEADAILISERLREGIASQAFKFDDVVIELT
VSIGVAALKPPQTRLTDLVNLADKGLYKAKFSGKNRVYLIEQFA
>Mature_684_residues
MILFFPALLGYYRLCFSLQLAPSLKPSTFCKIPSVNLVIQCVKLVGVDCKQTGFRVYIAKKWLKIITLFMVASSASVMSL
QVHAASPFLKLEQQLSDSSPSDALTLLNEFQLEPISTTAYNRAKLHFLYGKQYARNRLLEQSIASYDMAIVLAKPLMGSE
LLIESYLGRSFARYLQTNDPEVYCSDRAKALVLARRQSNRELLAKSLTQNAFCFNKVTNVHHGIALLDEAMLLIDTEDKA
NYNRKAMIFNATGSLYRTVGLHKRGYVNFEKAYQNWSEIDDTQDMFNMLHNMISEAVKLGDWEKARDNIEQQFLLAVKAS
EFNDFGFFAHLNAGRVELATHNYVDAIEHLEQAISLKDTTREQYFITSSYLFLAQAYLRSGDVEQAAAMARIFKQDSTFP
KNMSSMTLKADAIIALEQQQYLSAINALLEVIDKERQKNKQIIDNEVIDLALEHNSTLAEFENQLLANKLAINELSLAAV
ADKQRIYDLRVSLFFLVATVLLIAILFLWQSRKAFKHSAQTDFLTGIANRAHVFNRGQRMIDRAIKKKHPLAVIIFDIDN
FKSINDQYGHQVGDLAIKAVAKRGERWLRDCDLIGRIGGEEFLIVLPDVTEADAILISERLREGIASQAFKFDDVVIELT
VSIGVAALKPPQTRLTDLVNLADKGLYKAKFSGKNRVYLIEQFA

Specific function: Unknown

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=162, Percent_Identity=37.037037037037, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI1787262, Length=181, Percent_Identity=35.3591160220994, Blast_Score=112, Evalue=9e-26,
Organism=Escherichia coli, GI1786584, Length=187, Percent_Identity=32.620320855615, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI87082007, Length=197, Percent_Identity=32.48730964467, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI145693134, Length=167, Percent_Identity=33.5329341317365, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI87081881, Length=166, Percent_Identity=37.3493975903614, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787816, Length=170, Percent_Identity=29.4117647058824, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1788381, Length=176, Percent_Identity=30.1136363636364, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI87081977, Length=150, Percent_Identity=32, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1788085, Length=162, Percent_Identity=32.0987654320988, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1787541, Length=164, Percent_Identity=31.7073170731707, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI87081974, Length=189, Percent_Identity=29.6296296296296, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1788956, Length=169, Percent_Identity=33.7278106508876, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 77374; Mature: 77374

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILFFPALLGYYRLCFSLQLAPSLKPSTFCKIPSVNLVIQCVKLVGVDCKQTGFRVYIAK
CEEEHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCCEEEHHH
KWLKIITLFMVASSASVMSLQVHAASPFLKLEQQLSDSSPSDALTLLNEFQLEPISTTAY
HHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCC
NRAKLHFLYGKQYARNRLLEQSIASYDMAIVLAKPLMGSELLIESYLGRSFARYLQTNDP
CCEEEEEEECHHHHHHHHHHHHHHHHCHHEEEECCCCCHHHHHHHHHHHHHHHHHHCCCC
EVYCSDRAKALVLARRQSNRELLAKSLTQNAFCFNKVTNVHHGIALLDEAMLLIDTEDKA
CEEECCCHHEEHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCC
NYNRKAMIFNATGSLYRTVGLHKRGYVNFEKAYQNWSEIDDTQDMFNMLHNMISEAVKLG
CCCCEEEEEECCCCHHHHHCCCCCCCCCHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHCC
DWEKARDNIEQQFLLAVKASEFNDFGFFAHLNAGRVELATHNYVDAIEHLEQAISLKDTT
CHHHHHHHHHHHHHHEEECCCCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHCCC
REQYFITSSYLFLAQAYLRSGDVEQAAAMARIFKQDSTFPKNMSSMTLKADAIIALEQQQ
CCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEHHHH
YLSAINALLEVIDKERQKNKQIIDNEVIDLALEHNSTLAEFENQLLANKLAINELSLAAV
HHHHHHHHHHHHHHHHHHHHHHHCHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
ADKQRIYDLRVSLFFLVATVLLIAILFLWQSRKAFKHSAQTDFLTGIANRAHVFNRGQRM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IDRAIKKKHPLAVIIFDIDNFKSINDQYGHQVGDLAIKAVAKRGERWLRDCDLIGRIGGE
HHHHHHCCCCEEEEEEECCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EFLIVLPDVTEADAILISERLREGIASQAFKFDDVVIELTVSIGVAALKPPQTRLTDLVN
EEEEEECCCCCHHHHHHHHHHHHHHHHHCEECCCEEEEEEEHHCEEECCCCHHHHHHHHH
LADKGLYKAKFSGKNRVYLIEQFA
HHHCCCEEEEECCCCEEEEEEECC
>Mature Secondary Structure
MILFFPALLGYYRLCFSLQLAPSLKPSTFCKIPSVNLVIQCVKLVGVDCKQTGFRVYIAK
CEEEHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCCEEEHHH
KWLKIITLFMVASSASVMSLQVHAASPFLKLEQQLSDSSPSDALTLLNEFQLEPISTTAY
HHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCC
NRAKLHFLYGKQYARNRLLEQSIASYDMAIVLAKPLMGSELLIESYLGRSFARYLQTNDP
CCEEEEEEECHHHHHHHHHHHHHHHHCHHEEEECCCCCHHHHHHHHHHHHHHHHHHCCCC
EVYCSDRAKALVLARRQSNRELLAKSLTQNAFCFNKVTNVHHGIALLDEAMLLIDTEDKA
CEEECCCHHEEHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCC
NYNRKAMIFNATGSLYRTVGLHKRGYVNFEKAYQNWSEIDDTQDMFNMLHNMISEAVKLG
CCCCEEEEEECCCCHHHHHCCCCCCCCCHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHCC
DWEKARDNIEQQFLLAVKASEFNDFGFFAHLNAGRVELATHNYVDAIEHLEQAISLKDTT
CHHHHHHHHHHHHHHEEECCCCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHCCC
REQYFITSSYLFLAQAYLRSGDVEQAAAMARIFKQDSTFPKNMSSMTLKADAIIALEQQQ
CCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEHHHH
YLSAINALLEVIDKERQKNKQIIDNEVIDLALEHNSTLAEFENQLLANKLAINELSLAAV
HHHHHHHHHHHHHHHHHHHHHHHCHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
ADKQRIYDLRVSLFFLVATVLLIAILFLWQSRKAFKHSAQTDFLTGIANRAHVFNRGQRM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IDRAIKKKHPLAVIIFDIDNFKSINDQYGHQVGDLAIKAVAKRGERWLRDCDLIGRIGGE
HHHHHHCCCCEEEEEEECCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EFLIVLPDVTEADAILISERLREGIASQAFKFDDVVIELTVSIGVAALKPPQTRLTDLVN
EEEEEECCCCCHHHHHHHHHHHHHHHHHCEECCCEEEEEEEHHCEEECCCCHHHHHHHHH
LADKGLYKAKFSGKNRVYLIEQFA
HHHCCCEEEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]