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The map label for this gene is mepA [H]

Identifier: 157960372

GI number: 157960372

Start: 667460

End: 668344

Strand: Direct

Name: mepA [H]

Synonym: Spea_0543

Alternate gene names: 157960372

Gene position: 667460-668344 (Clockwise)

Preceding gene: 157960370

Following gene: 157960376

Centisome position: 12.9

GC content: 49.6

Gene sequence:

>885_bases
ATGATAGTGAGTCAGTTGCGAGCCTTGGGCTGGCAAGCCATGCTCCTGAGTCTGCTTGTTTGCTTAATCCTGTTTGTGGC
TCCTAAGCTACAGGCTAATGAGTGGGAGTCGGTAGAGACGCCTTATGCGAGTCACGCCAGTGCCATTGGCGGTTATGCTA
ATGGCTGTCTAACAGGCGCTCAGACTTTACCTCTAGATGGAGAAGGCTATCAAGTTATCCGCCCTCAGCGACAGAGATAC
TACGGCCATCCGGCGCTTATCGAATTTATTCAGCAGTTTGCCGCGAGTCTCAATCGGCAGGGGAGTGATGATATTTTAAT
TGCTGATATGTCTATGCCAAGGGGAGGCAACTTCACTTACGGCCACAGCAGCCATCAGATAGGGTTAGATGTCGACGTTT
GGTTCAAGCAAGCGAATCAGCCGCTAACTTCAGAGGAGCGAGCACAACCAATAGCGCTAAAACTCGTTGATAAGAAGCGG
TTTGCACTCGACACTCAGTTATGGTCGAAGCTGCAGACGCAGATGATTAAGATAGCGGCACTGGATGAGCGAGTGGCACG
AATTTTTGTCAGTCCAGTGATAAAGCAGCACCTGTGCGATTTAAGGCTAGAGAATGATACCTGGCTTAATAAGGTAAGGC
CTTGGTGGGGGCATACTTATCATATGCATGTCAGGCTGAGTTGCCCTAAAGACAATCCGCTCTGTCGGGATCAAGCTAGC
ATACCCGTTGGCAATGGTTGTCATGAGCTTGGCTGGTGGAAGCGTCAGTTGACGCAAACTCAGACTACTCAAGCTGCCAA
GGCTAACAAGCCCGCCAGCAAGCCTAAAAAGAAAAAAGCCAAGGTTAAACCTGAGCAGTGCTCGCGAATGATTGCAGCCA
AATAA

Upstream 100 bases:

>100_bases
CCCCCGTCTCTAGTTACGCCCTATAGCAATCTCAACTATCTTTAATGCAGTGCTCAATTCGAATGGGCAAGAGGTTATAG
CTAAGCTTTGGAGGAAAGGC

Downstream 100 bases:

>100_bases
AGGCCCGTAAAAACAAGCCGCCAACAGTGACTCTTCGGCGGCTTGTGATTTCAACACTTGATGAGCGCTACTGGCTTTAT
TAAAGGTACGCTAATGGCTT

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: D-alanyl-D-alanine-endopeptidase; DD-endopeptidase [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MIVSQLRALGWQAMLLSLLVCLILFVAPKLQANEWESVETPYASHASAIGGYANGCLTGAQTLPLDGEGYQVIRPQRQRY
YGHPALIEFIQQFAASLNRQGSDDILIADMSMPRGGNFTYGHSSHQIGLDVDVWFKQANQPLTSEERAQPIALKLVDKKR
FALDTQLWSKLQTQMIKIAALDERVARIFVSPVIKQHLCDLRLENDTWLNKVRPWWGHTYHMHVRLSCPKDNPLCRDQAS
IPVGNGCHELGWWKRQLTQTQTTQAAKANKPASKPKKKKAKVKPEQCSRMIAAK

Sequences:

>Translated_294_residues
MIVSQLRALGWQAMLLSLLVCLILFVAPKLQANEWESVETPYASHASAIGGYANGCLTGAQTLPLDGEGYQVIRPQRQRY
YGHPALIEFIQQFAASLNRQGSDDILIADMSMPRGGNFTYGHSSHQIGLDVDVWFKQANQPLTSEERAQPIALKLVDKKR
FALDTQLWSKLQTQMIKIAALDERVARIFVSPVIKQHLCDLRLENDTWLNKVRPWWGHTYHMHVRLSCPKDNPLCRDQAS
IPVGNGCHELGWWKRQLTQTQTTQAAKANKPASKPKKKKAKVKPEQCSRMIAAK
>Mature_294_residues
MIVSQLRALGWQAMLLSLLVCLILFVAPKLQANEWESVETPYASHASAIGGYANGCLTGAQTLPLDGEGYQVIRPQRQRY
YGHPALIEFIQQFAASLNRQGSDDILIADMSMPRGGNFTYGHSSHQIGLDVDVWFKQANQPLTSEERAQPIALKLVDKKR
FALDTQLWSKLQTQMIKIAALDERVARIFVSPVIKQHLCDLRLENDTWLNKVRPWWGHTYHMHVRLSCPKDNPLCRDQAS
IPVGNGCHELGWWKRQLTQTQTTQAAKANKPASKPKKKKAKVKPEQCSRMIAAK

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=259, Percent_Identity=41.3127413127413, Blast_Score=205, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 33179; Mature: 33179

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVSQLRALGWQAMLLSLLVCLILFVAPKLQANEWESVETPYASHASAIGGYANGCLTGA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHCCCC
QTLPLDGEGYQVIRPQRQRYYGHPALIEFIQQFAASLNRQGSDDILIADMSMPRGGNFTY
CCCCCCCCCCEEECCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCC
GHSSHQIGLDVDVWFKQANQPLTSEERAQPIALKLVDKKRFALDTQLWSKLQTQMIKIAA
CCCCCEECCCHHHHHHHCCCCCCCHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
LDERVARIFVSPVIKQHLCDLRLENDTWLNKVRPWWGHTYHMHVRLSCPKDNPLCRDQAS
HHHHHHHHHHHHHHHHHHHHCEECCCHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCC
IPVGNGCHELGWWKRQLTQTQTTQAAKANKPASKPKKKKAKVKPEQCSRMIAAK
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure
MIVSQLRALGWQAMLLSLLVCLILFVAPKLQANEWESVETPYASHASAIGGYANGCLTGA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHCCCC
QTLPLDGEGYQVIRPQRQRYYGHPALIEFIQQFAASLNRQGSDDILIADMSMPRGGNFTY
CCCCCCCCCCEEECCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCC
GHSSHQIGLDVDVWFKQANQPLTSEERAQPIALKLVDKKRFALDTQLWSKLQTQMIKIAA
CCCCCEECCCHHHHHHHCCCCCCCHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
LDERVARIFVSPVIKQHLCDLRLENDTWLNKVRPWWGHTYHMHVRLSCPKDNPLCRDQAS
HHHHHHHHHHHHHHHHHHHHCEECCCHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCC
IPVGNGCHELGWWKRQLTQTQTTQAAKANKPASKPKKKKAKVKPEQCSRMIAAK
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA