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The map label for this gene is rep [H]

Identifier: 157960189

GI number: 157960189

Start: 411859

End: 413874

Strand: Direct

Name: rep [H]

Synonym: Spea_0360

Alternate gene names: 157960189

Gene position: 411859-413874 (Clockwise)

Preceding gene: 157960184

Following gene: 157960203

Centisome position: 7.96

GC content: 46.97

Gene sequence:

>2016_bases
ATGAAGCTCAATCCCGGACAAAATGATGCCGTACACTATGTTAGTGGCCCTTGCTTAGTCCTAGCAGGCGCTGGCAGTGG
CAAGACCAGAGTGATCATTAATAAGATCGCCTATCTGGTGGAAAAGTGTGGCTATAAAGCACGCAATATTGCTGCGGTTA
CTTTTACAAATAAAGCGGCACGCGAAATGAAAGAGCGTGTATCACAGTCTATGGGGCGTAAAGAGGCGCGAGGATTATGG
ATCTCAACTTTCCATACTTTAGGCTTAGAGATTATTAAACGCGAGCATAAAGTAGTTGGCTTAAAGGCTGGCTTTTCGCT
GTTTGATGATCAAGATACGTTAGCGCTATTAAAGGAACTGACTCAGGATGAGTTAGACGAAGATAAAGATCTGCTGAGGC
TATTAGCCACCACCATATCCAACTGGAAAGGTGATTTAGTTATCCCTGAGCAGGCGCGTAAGATTGCCAAAGATGAACAG
CAGCAGCTGTTTGCGCTACTGTATCAGCGTTATGCTCAGCACATGAAGGCCTATAACGCGCTCGACTTCGATGATTTGAT
TCTATTACCGACTCTGTTATTGAGACAGAACGAAGAGGTAAGAACTCGCTGGCAGACTCGCATTCAATATATGCTGGTGG
ATGAGTATCAAGACACTAACACCAGCCAATATGAGATGGTTAAGTTGCTGGTGGGCGAGCGAGCTCGCTTTACCGTGGTA
GGCGATGATGATCAGTCTATCTATTCATGGCGTGGTGCTAAGCCACAAAACTTGGTGCTCTTGGGTAAAGACTTCCCGCG
ATTAAAATTGATTAAGCTGGAGCAAAACTATCGCTCTAGTCAGCGCATCTTGCGCGCAGCCAACATTCTTATTGCTAACA
ATCCGCACGTATATGAAAAGGCTCTATTCAGTGAGCTCGCTTATGGCGAACCTATCCGGGTGTTGATTGCTGCCAACGAG
GAGCAGGAAGCGGAGCGAGTCGTCGCCGAGATGATCCGTCACAAGTTCGTTGGTAGGACCTCATATGGGGATTATGCGAT
TCTGTATCGCGGTAACCATCAGTCGCGTCTACTCGAGCGTGCATTGATGACTAACCGTATTCCTTACAAACTCAGTGGTG
GCACCTCTTTCTTTGGTCGTGCCGAAATCAAAGACATCATGGCTTACCTGCGTTTGGTGGTTAACCCCGATGATGATAAT
GCTTTCTTGCGCATTGTTAACTTGCCTAAGCGAGGGATTGGTCCAGCAACGCTTGAGCGTCTAGGTAATTTTGCCAATAA
GAAGCAGATCTCGATGTTCGCTGCTATTTTTGAGGCCGACTTAAATCATGAATTACCGCCTGCTGCCATGTCGTCTCTGT
ATCAGTTTGGTCGTTTCGTGGTTGAAATGGGTGACTGTGCCGAACGTGGCGATCCCGTCGAAGCTGTTAAGCAGTTAATT
CGAAAGATTAATTACGAAGACTACCTATACGAGACCAGTACTAGCGCAAAAGCGGCTGAAATGCGCATGAAGAACATTTC
TGAGCTTTACCGCTGGGTAACAGAGATGCTGCAAGGCGACGATTTAGATGAAGGTATGACATTACCTGAGGTGGTTACTC
GTCTTACCCTGCGCGACATGATGGAGCGTAATAACGAAGATGAGGGCGGCGATCAGGTTCAACTTATGACCTTACACGCC
TCTAAGGGACTGGAATTTCCTTATGTGTTTATGGTGGGCGCCGAAGAGCGAATTTTGCCTCACCAAAGCAGTATCGATGA
AGATAACGTCGATGAAGAGCGTCGTCTGGCATATGTGGGGATCACCCGTGCACAAAGAGAGTTGTGGTTTGTGATTTGTC
GGGAGCGTCGCCAGTTTGGTGAAACCATTCGTTGTGAGCCGAGTCGCTTCTTGATGGAACTTCCTCAAGATGATCTCATT
TGGGAAAACCGTAAACCGGTGCAAACTGTAGAGCAGCGTCAGCAGTCTGGGAAAACGAATATCGCCAACATAAGAGATAT
GTTCAAGAATAAGTAG

Upstream 100 bases:

>100_bases
TAAGGAGGAGATATTGTCGATAACTTTTCCGCTTGCTGAGGCCTTTCTGCTATCATTCCTTTTTTAATGCCTTCCTTAAT
ACCTTGGTTATAACGTATCC

Downstream 100 bases:

>100_bases
TGCTTGTTTCTTAAAGCTGGGTATTGGCTTAAATTAGGCACATACCCTGTTTTTGTTTGTCTCTAACTCAATCACATTAC
CTAATAGGCTACCTTGGCCT

Product: ATP-dependent DNA helicase Rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 671

Protein sequence:

>671_residues
MKLNPGQNDAVHYVSGPCLVLAGAGSGKTRVIINKIAYLVEKCGYKARNIAAVTFTNKAAREMKERVSQSMGRKEARGLW
ISTFHTLGLEIIKREHKVVGLKAGFSLFDDQDTLALLKELTQDELDEDKDLLRLLATTISNWKGDLVIPEQARKIAKDEQ
QQLFALLYQRYAQHMKAYNALDFDDLILLPTLLLRQNEEVRTRWQTRIQYMLVDEYQDTNTSQYEMVKLLVGERARFTVV
GDDDQSIYSWRGAKPQNLVLLGKDFPRLKLIKLEQNYRSSQRILRAANILIANNPHVYEKALFSELAYGEPIRVLIAANE
EQEAERVVAEMIRHKFVGRTSYGDYAILYRGNHQSRLLERALMTNRIPYKLSGGTSFFGRAEIKDIMAYLRLVVNPDDDN
AFLRIVNLPKRGIGPATLERLGNFANKKQISMFAAIFEADLNHELPPAAMSSLYQFGRFVVEMGDCAERGDPVEAVKQLI
RKINYEDYLYETSTSAKAAEMRMKNISELYRWVTEMLQGDDLDEGMTLPEVVTRLTLRDMMERNNEDEGGDQVQLMTLHA
SKGLEFPYVFMVGAEERILPHQSSIDEDNVDEERRLAYVGITRAQRELWFVICRERRQFGETIRCEPSRFLMELPQDDLI
WENRKPVQTVEQRQQSGKTNIANIRDMFKNK

Sequences:

>Translated_671_residues
MKLNPGQNDAVHYVSGPCLVLAGAGSGKTRVIINKIAYLVEKCGYKARNIAAVTFTNKAAREMKERVSQSMGRKEARGLW
ISTFHTLGLEIIKREHKVVGLKAGFSLFDDQDTLALLKELTQDELDEDKDLLRLLATTISNWKGDLVIPEQARKIAKDEQ
QQLFALLYQRYAQHMKAYNALDFDDLILLPTLLLRQNEEVRTRWQTRIQYMLVDEYQDTNTSQYEMVKLLVGERARFTVV
GDDDQSIYSWRGAKPQNLVLLGKDFPRLKLIKLEQNYRSSQRILRAANILIANNPHVYEKALFSELAYGEPIRVLIAANE
EQEAERVVAEMIRHKFVGRTSYGDYAILYRGNHQSRLLERALMTNRIPYKLSGGTSFFGRAEIKDIMAYLRLVVNPDDDN
AFLRIVNLPKRGIGPATLERLGNFANKKQISMFAAIFEADLNHELPPAAMSSLYQFGRFVVEMGDCAERGDPVEAVKQLI
RKINYEDYLYETSTSAKAAEMRMKNISELYRWVTEMLQGDDLDEGMTLPEVVTRLTLRDMMERNNEDEGGDQVQLMTLHA
SKGLEFPYVFMVGAEERILPHQSSIDEDNVDEERRLAYVGITRAQRELWFVICRERRQFGETIRCEPSRFLMELPQDDLI
WENRKPVQTVEQRQQSGKTNIANIRDMFKNK
>Mature_671_residues
MKLNPGQNDAVHYVSGPCLVLAGAGSGKTRVIINKIAYLVEKCGYKARNIAAVTFTNKAAREMKERVSQSMGRKEARGLW
ISTFHTLGLEIIKREHKVVGLKAGFSLFDDQDTLALLKELTQDELDEDKDLLRLLATTISNWKGDLVIPEQARKIAKDEQ
QQLFALLYQRYAQHMKAYNALDFDDLILLPTLLLRQNEEVRTRWQTRIQYMLVDEYQDTNTSQYEMVKLLVGERARFTVV
GDDDQSIYSWRGAKPQNLVLLGKDFPRLKLIKLEQNYRSSQRILRAANILIANNPHVYEKALFSELAYGEPIRVLIAANE
EQEAERVVAEMIRHKFVGRTSYGDYAILYRGNHQSRLLERALMTNRIPYKLSGGTSFFGRAEIKDIMAYLRLVVNPDDDN
AFLRIVNLPKRGIGPATLERLGNFANKKQISMFAAIFEADLNHELPPAAMSSLYQFGRFVVEMGDCAERGDPVEAVKQLI
RKINYEDYLYETSTSAKAAEMRMKNISELYRWVTEMLQGDDLDEGMTLPEVVTRLTLRDMMERNNEDEGGDQVQLMTLHA
SKGLEFPYVFMVGAEERILPHQSSIDEDNVDEERRLAYVGITRAQRELWFVICRERRQFGETIRCEPSRFLMELPQDDLI
WENRKPVQTVEQRQQSGKTNIANIRDMFKNK

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=671, Percent_Identity=68.2563338301043, Blast_Score=931, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=641, Percent_Identity=38.8455538221529, Blast_Score=397, Evalue=1e-111,
Organism=Escherichia coli, GI1787196, Length=346, Percent_Identity=28.0346820809249, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322369, Length=438, Percent_Identity=32.4200913242009, Blast_Score=204, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77266; Mature: 77266

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNPGQNDAVHYVSGPCLVLAGAGSGKTRVIINKIAYLVEKCGYKARNIAAVTFTNKAA
CCCCCCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHH
REMKERVSQSMGRKEARGLWISTFHTLGLEIIKREHKVVGLKAGFSLFDDQDTLALLKEL
HHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHH
TQDELDEDKDLLRLLATTISNWKGDLVIPEQARKIAKDEQQQLFALLYQRYAQHMKAYNA
HHHHCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LDFDDLILLPTLLLRQNEEVRTRWQTRIQYMLVDEYQDTNTSQYEMVKLLVGERARFTVV
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCCEEEEE
GDDDQSIYSWRGAKPQNLVLLGKDFPRLKLIKLEQNYRSSQRILRAANILIANNPHVYEK
CCCCCHHHHCCCCCCCCEEEECCCCCCEEEEEEHHHHHHHHHHHHHHCEEEECCCCHHHH
ALFSELAYGEPIRVLIAANEEQEAERVVAEMIRHKFVGRTSYGDYAILYRGNHQSRLLER
HHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHH
ALMTNRIPYKLSGGTSFFGRAEIKDIMAYLRLVVNPDDDNAFLRIVNLPKRGIGPATLER
HHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHEECCCCCCEEEEEEECCCCCCCHHHHHH
LGNFANKKQISMFAAIFEADLNHELPPAAMSSLYQFGRFVVEMGDCAERGDPVEAVKQLI
HHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHH
RKINYEDYLYETSTSAKAAEMRMKNISELYRWVTEMLQGDDLDEGMTLPEVVTRLTLRDM
HHCCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
MERNNEDEGGDQVQLMTLHASKGLEFPYVFMVGAEERILPHQSSIDEDNVDEERRLAYVG
HHCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCCHHCCCCCCCCCCCCCCHHHEEEEEE
ITRAQRELWFVICRERRQFGETIRCEPSRFLMELPQDDLIWENRKPVQTVEQRQQSGKTN
HHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCC
IANIRDMFKNK
HHHHHHHHCCC
>Mature Secondary Structure
MKLNPGQNDAVHYVSGPCLVLAGAGSGKTRVIINKIAYLVEKCGYKARNIAAVTFTNKAA
CCCCCCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHH
REMKERVSQSMGRKEARGLWISTFHTLGLEIIKREHKVVGLKAGFSLFDDQDTLALLKEL
HHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHH
TQDELDEDKDLLRLLATTISNWKGDLVIPEQARKIAKDEQQQLFALLYQRYAQHMKAYNA
HHHHCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LDFDDLILLPTLLLRQNEEVRTRWQTRIQYMLVDEYQDTNTSQYEMVKLLVGERARFTVV
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCCEEEEE
GDDDQSIYSWRGAKPQNLVLLGKDFPRLKLIKLEQNYRSSQRILRAANILIANNPHVYEK
CCCCCHHHHCCCCCCCCEEEECCCCCCEEEEEEHHHHHHHHHHHHHHCEEEECCCCHHHH
ALFSELAYGEPIRVLIAANEEQEAERVVAEMIRHKFVGRTSYGDYAILYRGNHQSRLLER
HHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHH
ALMTNRIPYKLSGGTSFFGRAEIKDIMAYLRLVVNPDDDNAFLRIVNLPKRGIGPATLER
HHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHEECCCCCCEEEEEEECCCCCCCHHHHHH
LGNFANKKQISMFAAIFEADLNHELPPAAMSSLYQFGRFVVEMGDCAERGDPVEAVKQLI
HHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHH
RKINYEDYLYETSTSAKAAEMRMKNISELYRWVTEMLQGDDLDEGMTLPEVVTRLTLRDM
HHCCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
MERNNEDEGGDQVQLMTLHASKGLEFPYVFMVGAEERILPHQSSIDEDNVDEERRLAYVG
HHCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCCHHCCCCCCCCCCCCCCHHHEEEEEE
ITRAQRELWFVICRERRQFGETIRCEPSRFLMELPQDDLIWENRKPVQTVEQRQQSGKTN
HHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCC
IANIRDMFKNK
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]